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The map label for this gene is nuoB2 [H]

Identifier: 156937336

GI number: 156937336

Start: 487878

End: 488501

Strand: Reverse

Name: nuoB2 [H]

Synonym: Igni_0543

Alternate gene names: 156937336

Gene position: 488501-487878 (Counterclockwise)

Preceding gene: 156937337

Following gene: 156937335

Centisome position: 37.65

GC content: 59.62

Gene sequence:

>624_bases
GTGGACGCGATGAGGTTCAAGAAGGTCTCTCGCGAATTGATAAAGAAGAGCGCCCCCGTGAAGGAGGCGCTCAAGTGGAG
CCGCAGGAACAGCTTGTGGTTGCTGCACCTAGGCATAATGTGTTGTGCAATAGAGATGATGCAAGCGGCCTTCGCCTTCC
ACGACTTCGAGAGGCTCGGCGTGCTCGCTAGGGCGACCCCGAGGCAAGTGGACGTGATACTGATCAACGGCCCGGTGAAC
AGAAAACTCGCCGAGAGGCTCCGAAGGCTCTTCATGCAAACCCCGGGCCCGCGCTTCGTCATAGTTATGGGGGAGTGCGC
CATCAACGGCGGCCCGTGGTGGCAGAGCGACTACGTCTTGGACGGCATTCACGAGGTAATAGAGAGCTTGCCGGAGGAGT
ACCGGAAGGACGTCCACGTGGTCAACTTGGCCGGCTGCCCGCCCAGGCCGGAGGGGTTGTTGAGGGCGATACTGAAGATG
GAGGAGTTGATAAACGAAGGGGACCTCGCGAGGGCGCCCCTCACGCGTTATCGCCTACACGACGAGGAGGGCTCGGACAA
GAAGGTCGTGGACCCCATGCTCCGTTATGCCTACAACAACAGAAAGGCGGGTGAGAAGAGATGA

Upstream 100 bases:

>100_bases
CCTCCGGGAGTCCCAAGCCCAACACGTTGATAGGCATAGGATTGTTGTACTTCAGCTTGGTTGTTGGGCTGGTCTGGTTA
AGGAAAATAGGGATAATGAG

Downstream 100 bases:

>100_bases
ACTTCGTGGGTGAGGTCGAGAGGAAGGAGTGGAGCAACGCCCTATACGTCGAGGTAGAGCCCCAGTACTGGTACGAGAAC
GCGGCGAGGCTGAAGGACCA

Product: NADH-quinone oxidoreductase, B subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2 [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MDAMRFKKVSRELIKKSAPVKEALKWSRRNSLWLLHLGIMCCAIEMMQAAFAFHDFERLGVLARATPRQVDVILINGPVN
RKLAERLRRLFMQTPGPRFVIVMGECAINGGPWWQSDYVLDGIHEVIESLPEEYRKDVHVVNLAGCPPRPEGLLRAILKM
EELINEGDLARAPLTRYRLHDEEGSDKKVVDPMLRYAYNNRKAGEKR

Sequences:

>Translated_207_residues
MDAMRFKKVSRELIKKSAPVKEALKWSRRNSLWLLHLGIMCCAIEMMQAAFAFHDFERLGVLARATPRQVDVILINGPVN
RKLAERLRRLFMQTPGPRFVIVMGECAINGGPWWQSDYVLDGIHEVIESLPEEYRKDVHVVNLAGCPPRPEGLLRAILKM
EELINEGDLARAPLTRYRLHDEEGSDKKVVDPMLRYAYNNRKAGEKR
>Mature_207_residues
MDAMRFKKVSRELIKKSAPVKEALKWSRRNSLWLLHLGIMCCAIEMMQAAFAFHDFERLGVLARATPRQVDVILINGPVN
RKLAERLRRLFMQTPGPRFVIVMGECAINGGPWWQSDYVLDGIHEVIESLPEEYRKDVHVVNLAGCPPRPEGLLRAILKM
EELINEGDLARAPLTRYRLHDEEGSDKKVVDPMLRYAYNNRKAGEKR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=148, Percent_Identity=37.8378378378378, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1788624, Length=157, Percent_Identity=33.7579617834395, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=148, Percent_Identity=40.5405405405405, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI18859983, Length=147, Percent_Identity=38.7755102040816, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24642371, Length=147, Percent_Identity=38.7755102040816, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=38.0952380952381, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 23821; Mature: 23821

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAMRFKKVSRELIKKSAPVKEALKWSRRNSLWLLHLGIMCCAIEMMQAAFAFHDFERLG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLARATPRQVDVILINGPVNRKLAERLRRLFMQTPGPRFVIVMGECAINGGPWWQSDYVL
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCHHH
DGIHEVIESLPEEYRKDVHVVNLAGCPPRPEGLLRAILKMEELINEGDLARAPLTRYRLH
HHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
DEEGSDKKVVDPMLRYAYNNRKAGEKR
CCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDAMRFKKVSRELIKKSAPVKEALKWSRRNSLWLLHLGIMCCAIEMMQAAFAFHDFERLG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLARATPRQVDVILINGPVNRKLAERLRRLFMQTPGPRFVIVMGECAINGGPWWQSDYVL
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCHHH
DGIHEVIESLPEEYRKDVHVVNLAGCPPRPEGLLRAILKMEELINEGDLARAPLTRYRLH
HHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
DEEGSDKKVVDPMLRYAYNNRKAGEKR
CCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA