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The map label for this gene is purF [C]

Identifier: 156937201

GI number: 156937201

Start: 358802

End: 359692

Strand: Direct

Name: purF [C]

Synonym: Igni_0407

Alternate gene names: 156937201

Gene position: 358802-359692 (Clockwise)

Preceding gene: 156937200

Following gene: 156937202

Centisome position: 27.65

GC content: 56.45

Gene sequence:

>891_bases
TTGTGCGGCATCGCCGGTATAATGTTTAGTGACGGCAAGAAGGAGGTAGGCGAGCAACTTTACGAGATGCTCAAGAGCTT
GCAGCACAGGGGGATGGACTCGGCCGGCGTCATCGTTTACGACGACAAGCCCGGCGACTGGCTCGTGCGCGTGGTGGCGG
AGGACGGCGAGGAGGCGTTGAGCAAGATAAAGTATAGGCTAAACGTCAGCAAGTACGAGAAGCCCAAGAAGCTGAACGAC
GGGACCTACTTGATCGAGTTCACCGTCCCCCGGTCGGTCAAATTGGAGGAAATTAGGGAAGTGATAAGGAAGTTGAAGTA
TCCCATACTTTCGTTGGGCAGATACTCAGTCATCTCTAAGGACGTGGGTTTGGTGGACGACGTGAACAGGGTTTACGGCT
TCAAGGAGCAGCTGGGCACCCACGGCATAGGGCACGTGAGGTTCTCCACGGAGAGCGCGGTGGACGCGATTCACGCCCAC
CCCTTCCAGACTCCGGAGTTCCCGGACATCGCGATAGTCCACAACGGCCAGATAACCAACTACTGGAAGATGAGGGAGCT
GTTGGAGCTGGAGGGCCACGTGTTCCAGACTGACAACGACTCGGAGCTCATTATACACTACATAGTGAATAAGTTGAGGA
AGGGGTACGAGCTGGAAGAAGCTCTCAAGTCTGCCGTAAACGACCTGGACGGCCCGTTCGCCTTCTTGCTCTCCATGCCG
GAGGGCATAGGCATGGCGAGGGACAGGCTGGGGTTAAGGCCTTTAGTGGTCGCTGAGGGCGACGGGGTGCTCGCGGCCGC
CTCGGAGGAGGTGGCCATTAGGAAGATCTTACCCGAGTCCAAGATATACTACATGAGGCCCGGAGAGGTGAGAGTATGGA
TGAGGCAATAA

Upstream 100 bases:

>100_bases
TGGAGGAGTTAAGGGTCGGCCTAGAGACGATTAAGGAAGTCCTCGGGAGTTAAATAGGAGAGGTTAAAGAGGCTCGGTTG
TCAGAAGGCGGGAGCGGCCC

Downstream 100 bases:

>100_bases
TAGACGCCTCCGCGCTGACCCCGAGGGAAATAAACAAGAAGCTGAGGGAGTACGCCAAGCAGTACAAGAGGATCATAATT
AGAAACCCCAACGCCAAGCA

Product: glutamate synthase (NADPH) GltB1 subunit

Products: L-glutamine; 5-phospho-alpha-D-ribose 1-diphosphate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MCGIAGIMFSDGKKEVGEQLYEMLKSLQHRGMDSAGVIVYDDKPGDWLVRVVAEDGEEALSKIKYRLNVSKYEKPKKLND
GTYLIEFTVPRSVKLEEIREVIRKLKYPILSLGRYSVISKDVGLVDDVNRVYGFKEQLGTHGIGHVRFSTESAVDAIHAH
PFQTPEFPDIAIVHNGQITNYWKMRELLELEGHVFQTDNDSELIIHYIVNKLRKGYELEEALKSAVNDLDGPFAFLLSMP
EGIGMARDRLGLRPLVVAEGDGVLAAASEEVAIRKILPESKIYYMRPGEVRVWMRQ

Sequences:

>Translated_296_residues
MCGIAGIMFSDGKKEVGEQLYEMLKSLQHRGMDSAGVIVYDDKPGDWLVRVVAEDGEEALSKIKYRLNVSKYEKPKKLND
GTYLIEFTVPRSVKLEEIREVIRKLKYPILSLGRYSVISKDVGLVDDVNRVYGFKEQLGTHGIGHVRFSTESAVDAIHAH
PFQTPEFPDIAIVHNGQITNYWKMRELLELEGHVFQTDNDSELIIHYIVNKLRKGYELEEALKSAVNDLDGPFAFLLSMP
EGIGMARDRLGLRPLVVAEGDGVLAAASEEVAIRKILPESKIYYMRPGEVRVWMRQ
>Mature_296_residues
MCGIAGIMFSDGKKEVGEQLYEMLKSLQHRGMDSAGVIVYDDKPGDWLVRVVAEDGEEALSKIKYRLNVSKYEKPKKLND
GTYLIEFTVPRSVKLEEIREVIRKLKYPILSLGRYSVISKDVGLVDDVNRVYGFKEQLGTHGIGHVRFSTESAVDAIHAH
PFQTPEFPDIAIVHNGQITNYWKMRELLELEGHVFQTDNDSELIIHYIVNKLRKGYELEEALKSAVNDLDGPFAFLLSMP
EGIGMARDRLGLRPLVVAEGDGVLAAASEEVAIRKILPESKIYYMRPGEVRVWMRQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0067

COG function: function code E; Glutamate synthase domain 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788651, Length=202, Percent_Identity=32.1782178217822, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790167, Length=162, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21357745, Length=115, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: 2.4.2.14

Molecular weight: Translated: 33510; Mature: 33510

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIMFSDGKKEVGEQLYEMLKSLQHRGMDSAGVIVYDDKPGDWLVRVVAEDGEEAL
CCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEHHCCHHHH
SKIKYRLNVSKYEKPKKLNDGTYLIEFTVPRSVKLEEIREVIRKLKYPILSLGRYSVISK
HHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHCCCHHHHHC
DVGLVDDVNRVYGFKEQLGTHGIGHVRFSTESAVDAIHAHPFQTPEFPDIAIVHNGQITN
CCCCHHHHHHHHCHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCEEEEECCCCCH
YWKMRELLELEGHVFQTDNDSELIIHYIVNKLRKGYELEEALKSAVNDLDGPFAFLLSMP
HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCC
EGIGMARDRLGLRPLVVAEGDGVLAAASEEVAIRKILPESKIYYMRPGEVRVWMRQ
CCCCHHHHHCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCEEEECCCCEEEEEEC
>Mature Secondary Structure
MCGIAGIMFSDGKKEVGEQLYEMLKSLQHRGMDSAGVIVYDDKPGDWLVRVVAEDGEEAL
CCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEHHCCHHHH
SKIKYRLNVSKYEKPKKLNDGTYLIEFTVPRSVKLEEIREVIRKLKYPILSLGRYSVISK
HHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHCCCHHHHHC
DVGLVDDVNRVYGFKEQLGTHGIGHVRFSTESAVDAIHAHPFQTPEFPDIAIVHNGQITN
CCCCHHHHHHHHCHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCEEEEECCCCCH
YWKMRELLELEGHVFQTDNDSELIIHYIVNKLRKGYELEEALKSAVNDLDGPFAFLLSMP
HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCC
EGIGMARDRLGLRPLVVAEGDGVLAAASEEVAIRKILPESKIYYMRPGEVRVWMRQ
CCCCHHHHHCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Mg-phosphoribosyldiphosphate Mg-phosphoribosyldiphosphate, [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 8.8 {NH3}} 0.067 {5-phospho-alpha-D-ribose} 1.7 {glutamine}} [C]

Substrates: 5-phospho-beta-D-ribosylamine; diphosphate; L-glutamate

Specific reaction: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O

General reaction: Pentosyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2-Mercaptoethanol; 6-Diazo-5-oxo-L-norleucine analogue [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]