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The map label for this gene is purD [H]

Identifier: 156936960

GI number: 156936960

Start: 148667

End: 150052

Strand: Direct

Name: purD [H]

Synonym: Igni_0165

Alternate gene names: 156936960

Gene position: 148667-150052 (Clockwise)

Preceding gene: 156936958

Following gene: 156936961

Centisome position: 11.46

GC content: 57.79

Gene sequence:

>1386_bases
TTGGCCGGCGAGAGGGTCCTCTTGGTCGGCGGCGGCGGGAGGGAACACGCTATCGGAGAGCGCTTGGTGAGCCACGGGGC
CGTGCTGGGCGTCGCGGCCTCCAATTTGAACCCCGGACTGTTGTCCCTTACGAAGGACTCCGGAGGCGAGTTCTTTAAAG
CGAATCCGACCAGCCCTCAAGAGGTACTCGAGGTTGCTAAGAAGTTCAAGGCGGAGCTCGTGGTGATAGGACCCGAAGAG
CCGCAGTTCAGGGGGGTCACGGACGCCCTAAGGGCGGCGGGCTTCACGGTCTTCGGGGCTTCCTCGAAGGCGTCAGAAAT
AGAGAAGAGTAAGGTCTTCGCAAGGAGTATAATGAAGAAGTACAACATCCCGGGCAACTTGGACTTCTGGTTCTTCGAGA
GCTGGGAGGAGGCAATAGAATTCTTAGAAAGAGCCGGGAACGTGGCGATAAAGCCGGCCAGGCAAGCCGGGGGCAAAGGA
GTTAAGGTAATCTCCGATGTGCAAGCGTACCTGAGCGAGGAGAAGAAGAAAGTGAAGGAGGAACACGCCAAAAGGATACT
GACCGAGGTTATGGCGAAGTATACAGACATCAAAGAGAAGTTGTTAATAGAGGAGAAGGTGGACGGCGTGGAGTATACTG
TCCAAGCGTTTACCGACGGCGAGCGCGTAGTGCCGTTGCCGGCAGTACAAGACAACCCCCACCTATTCGTCTACGACGTG
GGGCCCGAGACGGGCGGCATGGGAAGCGTTCAAGGCCCCGGTAAGCTACTGCCGTTCATAACCGAGGAGGAGTACAAAGT
AGCCGTTGAGATCCTCGAGAGGGTGGTAGAGGCAATAGCTAAGGAGACTGGTGAGAAATACGTAGGCGCGATCTCCGCAC
AGATGATGTTAACCACAAAGGGCCCGGTCCTAATAGAGTTCTACTCGAGGCTGGGAGACCCAGAGGCTGTCAACGTAATG
AACGTCGTCGAGGGAAACGTGTTGGAAGCGATGTTCGAAGCGGCCCAAGGGAACTTGAAGACAGAGCTCGGGGTTAGGGA
GGCGGCGAGTGCGGTCAAGTGTATAGCGCCCATGGGTTACCCCGAGCGGAGGGATCTCACCAAGGGTAGGGAAGTGCTCG
TCCCGGAGTGTGAGGGGGCGAAGGTGTACTACGCCTCCGTGGACGTCAAGGACGGTAAGCTGGTTAGTGGCGGGTCGAGG
GCCGTGGAGGTGTACGCGGAGGGTAAGAGCATAAGCGAGGCGGCGGAAAAGGCCAACGCGTGCGCGTCCAAGGTGAAGTT
CGTGAACTGGAAGAGCGTGTGGAGGCCCGATATAGGCGGTGAGTGGTACATAAGGAGGAGGAGCGAGGTGGCCGAACTAA
TGAGGTCGGTCTGGGGCTTGAGGTAG

Upstream 100 bases:

>100_bases
ATGCGGGTTTTTTCTTTAACGTTGGCCGGAACGAGCATGATATCCCACCTAGGAGCCTATTTAGGGCCAAAAAAGTTGGA
CGCTTCGAGGGATATAAGGT

Downstream 100 bases:

>100_bases
AGGTTTCGAAAGGAAATTAATTTTGAGAAAGTTTGGCCTTAAGCGGAGCAAGGACAATGGTGGAGACGTCTGTAGAGCTG
AGCAAAGTGGCGTTTTACGT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAGERVLLVGGGGREHAIGERLVSHGAVLGVAASNLNPGLLSLTKDSGGEFFKANPTSPQEVLEVAKKFKAELVVIGPEE
PQFRGVTDALRAAGFTVFGASSKASEIEKSKVFARSIMKKYNIPGNLDFWFFESWEEAIEFLERAGNVAIKPARQAGGKG
VKVISDVQAYLSEEKKKVKEEHAKRILTEVMAKYTDIKEKLLIEEKVDGVEYTVQAFTDGERVVPLPAVQDNPHLFVYDV
GPETGGMGSVQGPGKLLPFITEEEYKVAVEILERVVEAIAKETGEKYVGAISAQMMLTTKGPVLIEFYSRLGDPEAVNVM
NVVEGNVLEAMFEAAQGNLKTELGVREAASAVKCIAPMGYPERRDLTKGREVLVPECEGAKVYYASVDVKDGKLVSGGSR
AVEVYAEGKSISEAAEKANACASKVKFVNWKSVWRPDIGGEWYIRRRSEVAELMRSVWGLR

Sequences:

>Translated_461_residues
MAGERVLLVGGGGREHAIGERLVSHGAVLGVAASNLNPGLLSLTKDSGGEFFKANPTSPQEVLEVAKKFKAELVVIGPEE
PQFRGVTDALRAAGFTVFGASSKASEIEKSKVFARSIMKKYNIPGNLDFWFFESWEEAIEFLERAGNVAIKPARQAGGKG
VKVISDVQAYLSEEKKKVKEEHAKRILTEVMAKYTDIKEKLLIEEKVDGVEYTVQAFTDGERVVPLPAVQDNPHLFVYDV
GPETGGMGSVQGPGKLLPFITEEEYKVAVEILERVVEAIAKETGEKYVGAISAQMMLTTKGPVLIEFYSRLGDPEAVNVM
NVVEGNVLEAMFEAAQGNLKTELGVREAASAVKCIAPMGYPERRDLTKGREVLVPECEGAKVYYASVDVKDGKLVSGGSR
AVEVYAEGKSISEAAEKANACASKVKFVNWKSVWRPDIGGEWYIRRRSEVAELMRSVWGLR
>Mature_460_residues
AGERVLLVGGGGREHAIGERLVSHGAVLGVAASNLNPGLLSLTKDSGGEFFKANPTSPQEVLEVAKKFKAELVVIGPEEP
QFRGVTDALRAAGFTVFGASSKASEIEKSKVFARSIMKKYNIPGNLDFWFFESWEEAIEFLERAGNVAIKPARQAGGKGV
KVISDVQAYLSEEKKKVKEEHAKRILTEVMAKYTDIKEKLLIEEKVDGVEYTVQAFTDGERVVPLPAVQDNPHLFVYDVG
PETGGMGSVQGPGKLLPFITEEEYKVAVEILERVVEAIAKETGEKYVGAISAQMMLTTKGPVLIEFYSRLGDPEAVNVMN
VVEGNVLEAMFEAAQGNLKTELGVREAASAVKCIAPMGYPERRDLTKGREVLVPECEGAKVYYASVDVKDGKLVSGGSRA
VEVYAEGKSISEAAEKANACASKVKFVNWKSVWRPDIGGEWYIRRRSEVAELMRSVWGLR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=448, Percent_Identity=33.2589285714286, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI4503915, Length=445, Percent_Identity=33.2584269662921, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI209869995, Length=445, Percent_Identity=33.2584269662921, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI209869993, Length=445, Percent_Identity=33.2584269662921, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1790438, Length=452, Percent_Identity=29.8672566371681, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17567511, Length=445, Percent_Identity=28.0898876404494, Blast_Score=139, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321203, Length=454, Percent_Identity=26.8722466960352, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI62471619, Length=447, Percent_Identity=31.9910514541387, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24582400, Length=447, Percent_Identity=31.9910514541387, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 50268; Mature: 50137

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGERVLLVGGGGREHAIGERLVSHGAVLGVAASNLNPGLLSLTKDSGGEFFKANPTSPQ
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCCHH
EVLEVAKKFKAELVVIGPEEPQFRGVTDALRAAGFTVFGASSKASEIEKSKVFARSIMKK
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHH
YNIPGNLDFWFFESWEEAIEFLERAGNVAIKPARQAGGKGVKVISDVQAYLSEEKKKVKE
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EHAKRILTEVMAKYTDIKEKLLIEEKVDGVEYTVQAFTDGERVVPLPAVQDNPHLFVYDV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEEC
GPETGGMGSVQGPGKLLPFITEEEYKVAVEILERVVEAIAKETGEKYVGAISAQMMLTTK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEECC
GPVLIEFYSRLGDPEAVNVMNVVEGNVLEAMFEAAQGNLKTELGVREAASAVKCIAPMGY
CCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
PERRDLTKGREVLVPECEGAKVYYASVDVKDGKLVSGGSRAVEVYAEGKSISEAAEKANA
CCCCCCCCCCEEEEECCCCCEEEEEEEECCCCEEECCCCCEEEEEECCCHHHHHHHHHHH
CASKVKFVNWKSVWRPDIGGEWYIRRRSEVAELMRSVWGLR
HHHHHEEECHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGERVLLVGGGGREHAIGERLVSHGAVLGVAASNLNPGLLSLTKDSGGEFFKANPTSPQ
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCCHH
EVLEVAKKFKAELVVIGPEEPQFRGVTDALRAAGFTVFGASSKASEIEKSKVFARSIMKK
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHH
YNIPGNLDFWFFESWEEAIEFLERAGNVAIKPARQAGGKGVKVISDVQAYLSEEKKKVKE
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EHAKRILTEVMAKYTDIKEKLLIEEKVDGVEYTVQAFTDGERVVPLPAVQDNPHLFVYDV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEEC
GPETGGMGSVQGPGKLLPFITEEEYKVAVEILERVVEAIAKETGEKYVGAISAQMMLTTK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEECC
GPVLIEFYSRLGDPEAVNVMNVVEGNVLEAMFEAAQGNLKTELGVREAASAVKCIAPMGY
CCHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
PERRDLTKGREVLVPECEGAKVYYASVDVKDGKLVSGGSRAVEVYAEGKSISEAAEKANA
CCCCCCCCCCEEEEECCCCCEEEEEEEECCCCEEECCCCCEEEEEECCCHHHHHHHHHHH
CASKVKFVNWKSVWRPDIGGEWYIRRRSEVAELMRSVWGLR
HHHHHEEECHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]