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The map label for this gene is priB [H]

Identifier: 156936873

GI number: 156936873

Start: 74402

End: 75472

Strand: Direct

Name: priB [H]

Synonym: Igni_0078

Alternate gene names: 156936873

Gene position: 74402-75472 (Clockwise)

Preceding gene: 156936866

Following gene: 156936876

Centisome position: 5.73

GC content: 53.5

Gene sequence:

>1071_bases
TTGTTGTACGCGCGCTACCCCTTCGTTGGCAGCGTATCCAAGCTTTCTAAGAGGATGTTTGGCGTCGAGATGGACGTAGA
GGAACTGCTTGACCTATATCCAAATGCCATTGATTTAGCTAAAGCGATGGTAGAGGGGAGGGGTCCAGCCGAAGACGCCG
GTTGTGACGACTTGTGTGCGGTCTTGGCTTACAGAATAGCCTTAGTCCTCGTAGCGGCTGCCAGAAGCAATTGGTTGAGG
AACAACTTTGCAGTAAACGTAGCCAAGCGTGCGAGCGAGTTCTTTAAGAAAGAGGGCATAGAGACCGTCGTAGGGGTGTT
GAAGGCCTGCGGCTTCTGGGCGGAAGTCTTGAGGGAACCCTTGAGGGTGCCCTTGAGGCAACAGAGGGGAGCGGTCTACG
CCGAGGAGTACTTGGTGAGGACGACACTAATGGATTACGTCAGGTTGGCCAAGAGGTTCCTCAGCGACCCCACTTGGAAG
GTGGTTAACAAGGCCTTGTTAGGAGGCTTCGTCTACTTAAAGGAGGAGGAGCTGCGGAGGCTTGCAGAAGAAGCGGTCGT
AATTAAAGTTCTCGAAGACTTGAGGGAACTCGGCGACGTCGACCCCCAAGCCTTGCCAGAGAGGTTCAAAGGGGTGCTGG
AGGAGCTCAAAACGAAATACATGGCGAAGAGAAGTAGCGCGGATATCAAGATAAAGGGGTTCGAGCCGAGGGCGCTTCCT
CCGTGCGTCCGTTCGATTTACGAGAGGGCCCTCCAAGGGGATAACCTCTCGCATCAAGAGAGGTTCACACTAGCTACGTT
CTTATTGAACGTAGGTAAGGACGTAGAAGACGTATTGGAAGTTTTCAAGAACATGCCCGATTACAACGAGCGCATAGCGC
GTTACCAAGTGGAGCACTTGGCGGGCCTCAGAGGCTCACATAAGAAATATTCTCCACCTTCGTGTAAGACTTTAGTCTCG
TGGGGACTGTGCCCAGGAAAAGACGAGTGTAAGGGCGATCACCCGTTGAAGGAGTACTGGAGGAGGCTCCGCAGGCTCAC
CAAGGCTCCCTCTCGGGAACCACGACCTTAA

Upstream 100 bases:

>100_bases
GAGGGGATGAGCTAGAACTTCCGGCAGCGAACGGTGAGCGACTCTGCCAGCGGCGACCCCCTTTAAGCGTCAAAGCCCAT
CTGGCTAACGGAACGTCGAG

Downstream 100 bases:

>100_bases
CCCCCAAGGCCTCGGCCAACTCCTTGAACCTCAGAGCATCATCTATCGAACCCACTATAGTTCCCCAATGCATGGGTATT
GCTACTTGCACTCCCTTTAT

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLYARYPFVGSVSKLSKRMFGVEMDVEELLDLYPNAIDLAKAMVEGRGPAEDAGCDDLCAVLAYRIALVLVAAARSNWLR
NNFAVNVAKRASEFFKKEGIETVVGVLKACGFWAEVLREPLRVPLRQQRGAVYAEEYLVRTTLMDYVRLAKRFLSDPTWK
VVNKALLGGFVYLKEEELRRLAEEAVVIKVLEDLRELGDVDPQALPERFKGVLEELKTKYMAKRSSADIKIKGFEPRALP
PCVRSIYERALQGDNLSHQERFTLATFLLNVGKDVEDVLEVFKNMPDYNERIARYQVEHLAGLRGSHKKYSPPSCKTLVS
WGLCPGKDECKGDHPLKEYWRRLRRLTKAPSREPRP

Sequences:

>Translated_356_residues
MLYARYPFVGSVSKLSKRMFGVEMDVEELLDLYPNAIDLAKAMVEGRGPAEDAGCDDLCAVLAYRIALVLVAAARSNWLR
NNFAVNVAKRASEFFKKEGIETVVGVLKACGFWAEVLREPLRVPLRQQRGAVYAEEYLVRTTLMDYVRLAKRFLSDPTWK
VVNKALLGGFVYLKEEELRRLAEEAVVIKVLEDLRELGDVDPQALPERFKGVLEELKTKYMAKRSSADIKIKGFEPRALP
PCVRSIYERALQGDNLSHQERFTLATFLLNVGKDVEDVLEVFKNMPDYNERIARYQVEHLAGLRGSHKKYSPPSCKTLVS
WGLCPGKDECKGDHPLKEYWRRLRRLTKAPSREPRP
>Mature_356_residues
MLYARYPFVGSVSKLSKRMFGVEMDVEELLDLYPNAIDLAKAMVEGRGPAEDAGCDDLCAVLAYRIALVLVAAARSNWLR
NNFAVNVAKRASEFFKKEGIETVVGVLKACGFWAEVLREPLRVPLRQQRGAVYAEEYLVRTTLMDYVRLAKRFLSDPTWK
VVNKALLGGFVYLKEEELRRLAEEAVVIKVLEDLRELGDVDPQALPERFKGVLEELKTKYMAKRSSADIKIKGFEPRALP
PCVRSIYERALQGDNLSHQERFTLATFLLNVGKDVEDVLEVFKNMPDYNERIARYQVEHLAGLRGSHKKYSPPSCKTLVS
WGLCPGKDECKGDHPLKEYWRRLRRLTKAPSREPRP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 40598; Mature: 40598

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYARYPFVGSVSKLSKRMFGVEMDVEELLDLYPNAIDLAKAMVEGRGPAEDAGCDDLCA
CCEECCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
VLAYRIALVLVAAARSNWLRNNFAVNVAKRASEFFKKEGIETVVGVLKACGFWAEVLREP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRVPLRQQRGAVYAEEYLVRTTLMDYVRLAKRFLSDPTWKVVNKALLGGFVYLKEEELRR
HCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECHHHHHH
LAEEAVVIKVLEDLRELGDVDPQALPERFKGVLEELKTKYMAKRSSADIKIKGFEPRALP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PCVRSIYERALQGDNLSHQERFTLATFLLNVGKDVEDVLEVFKNMPDYNERIARYQVEHL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
AGLRGSHKKYSPPSCKTLVSWGLCPGKDECKGDHPLKEYWRRLRRLTKAPSREPRP
HCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLYARYPFVGSVSKLSKRMFGVEMDVEELLDLYPNAIDLAKAMVEGRGPAEDAGCDDLCA
CCEECCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
VLAYRIALVLVAAARSNWLRNNFAVNVAKRASEFFKKEGIETVVGVLKACGFWAEVLREP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRVPLRQQRGAVYAEEYLVRTTLMDYVRLAKRFLSDPTWKVVNKALLGGFVYLKEEELRR
HCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECHHHHHH
LAEEAVVIKVLEDLRELGDVDPQALPERFKGVLEELKTKYMAKRSSADIKIKGFEPRALP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PCVRSIYERALQGDNLSHQERFTLATFLLNVGKDVEDVLEVFKNMPDYNERIARYQVEHL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
AGLRGSHKKYSPPSCKTLVSWGLCPGKDECKGDHPLKEYWRRLRRLTKAPSREPRP
HCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]