Click here to switch to the map view.

The map label for this gene is gatE [H]

Identifier: 156936872

GI number: 156936872

Start: 72307

End: 74220

Strand: Reverse

Name: gatE [H]

Synonym: Igni_0077

Alternate gene names: 156936872

Gene position: 74220-72307 (Counterclockwise)

Preceding gene: 156936874

Following gene: 156936871

Centisome position: 5.72

GC content: 53.08

Gene sequence:

>1914_bases
TTGAATCTGAACTACGAGGAGCTGGGCTTAAAGGTAGGCCTAGAGATCCACCAGCAGCTGAAGACAGAAAGAAAGCTCTT
CTGTCACTGCAAGCCCGAGCTGGCCGAGGAAGAAGAGGACTTCGACGTAATAGAGAGGAGGCTGAGGCCGGTAAGGAGCG
AGCTGGGCGAGGTAGACGTGGCGGCCCTCTTCGAATGGAAGAAGGGTAGGACTTACGTCTACAGGGCACCGGTGGGGCAC
TCTTGTCTGGTGGAAGCGGACGAAGAGCCGCCCCACGAAGCCGACCGGGATTCAATAATCATTGCGTTGGCCATTGCCAA
GGCATTCAATTCGAAAATAATTGACGAAATTCACGTTATGAGGAAAATGGTAATAGACGGTTCCAACACGTCCGGCTTCC
AAAGGACTATGTTGGTAGCCCTCGGAGGGGAGCTAAACTTAGGGGACAAGAAGATAGGAATAGAAACAATATGCGTAGAG
GAGGACGCGGCGAGGAAGGTGGAAGAGAAGGAAAAGGTTACCGTGTTTAACTTGGACCGCTTGGGAATACCCTTAATAGA
GATAGCCACGGAGCCTACGATAACCTCTCCCCAAGAGGCCAAGAGGGTAGCGTTGAGAATAGGCCAGATGTTGAGGCTCA
CCGGAAGGGTAAAGAGGGGCTTGGGCACAATAAGGCAAGACCTCAACGTCTCCATTAAGGGAGGCACCAAGACAGAGATA
AAGGGCGTTCAGAAGCTCCAGATAATAGACAAGGTCGTAGAGTACGAAGTAATCAGACAGTTGAGGCTGCTCGAGATAAG
GGACGAGCTGAAGAGAAGGGGGGTAAAAGAGGAAGAAATCCTCTCCCAACCAATTAAGGACGTAACTGAAATATTCAAGG
ATACTGAGAGCAAGTTGATCAAGAGGTCGTTATCCAAGGGCAGCAGAGTCTATGCAATTAAACTTCCCAAGTTCAAGGGC
CTCCTCGGGAAAGAGGTCCAGCCGAACCGGAGGTTCGGAACGGAGCTCGCGGACTACGCTAGGTTCTGGGCGGGCGTAGG
GGGGATAATTCACAGCGACGAGCTCCCGGGCTACGGGATCTCCGAAGACGAGGTAGAGGACTTGAAAGAGGCCTTGGGGT
GCGGCGAAGAAGACGCTTTCGTGATGGTAATAGCGCCGCCGCCCGACGCGAAGAAGGCCTTAGAAGCTGTCAAGGAGAGG
GCTGCGTACGCCCTCAAAGGGGTGCCGGAGGAGACTAGGGCCGCGAACCCCGACGGGACGACCCGGTTCTTGAGGCCCAG
GCCGGGCTCCGCGAGGATGTATCCCGAAACCGACGTGCCTCCGATAGTGGTCACTGAAGACATGCTAACTAAAGCGGAGA
AGCTGAAGCCGAAGAACCCTATAGAATTAATCCAGGAATTGATCACTGACTATAAACTTTCAAAGGACATGGCAGAGAAG
GTCATATCCAGCGAACGCTTCGATTTGGCCTACAGACTCATCAAAAAGTACAAAAACAAGCTGAGTGCCAACTTGGTGGC
TAGGACCGTAGTGGACACCTTGAGGGAGTTGGAGAGGGAAGGACTCGAGACTGACAAGATTTCCGACGAAGCGATAGAGA
AGGTCCTAGACGAAGTCGCGGAGGGTAAGGTAGCTAAGGAAGCCATTCCGGAGGTCCTCCGCAAGGTCGCGGGTGGAGCT
AGTGTGGAAGAGGCGTTGAAGGAGTTCGTCACGGCTAGCCAGATCAACGTGGAAGAGGTAGTGAAGAAGGTAATACAGCA
AAACATAGAGGAGATAAAGAAGAGGGGAGTAGAAAGGAGCTTCGGCTTGGTCATGGGGAAAGCGATGGCCGAGCTTAGGG
GCAAGGTCGACGGGAAGGTCGTAGCCGAAACCGTAAAGAGGCTATTGAAAGAACTGGGTGAAGAGGGTGGCTGA

Upstream 100 bases:

>100_bases
AGTTCTAGCTCATCCCCTCGTCATTAAGGCCTACTACGACGGGGCGCGGGCCCCGTGACACACCTTATATCCCGAGGTCG
GGCACGGAGGAGGGGTCTAC

Downstream 100 bases:

>100_bases
CGCGCTACGCGTCACGATAGCTGCAGTCCTTTTGTTAACAGCTATAGGGATAATATCTCAAGTGGGCAACTTCCTAGCCT
TGATTCTGAAGTTCCCTCAA

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MNLNYEELGLKVGLEIHQQLKTERKLFCHCKPELAEEEEDFDVIERRLRPVRSELGEVDVAALFEWKKGRTYVYRAPVGH
SCLVEADEEPPHEADRDSIIIALAIAKAFNSKIIDEIHVMRKMVIDGSNTSGFQRTMLVALGGELNLGDKKIGIETICVE
EDAARKVEEKEKVTVFNLDRLGIPLIEIATEPTITSPQEAKRVALRIGQMLRLTGRVKRGLGTIRQDLNVSIKGGTKTEI
KGVQKLQIIDKVVEYEVIRQLRLLEIRDELKRRGVKEEEILSQPIKDVTEIFKDTESKLIKRSLSKGSRVYAIKLPKFKG
LLGKEVQPNRRFGTELADYARFWAGVGGIIHSDELPGYGISEDEVEDLKEALGCGEEDAFVMVIAPPPDAKKALEAVKER
AAYALKGVPEETRAANPDGTTRFLRPRPGSARMYPETDVPPIVVTEDMLTKAEKLKPKNPIELIQELITDYKLSKDMAEK
VISSERFDLAYRLIKKYKNKLSANLVARTVVDTLRELEREGLETDKISDEAIEKVLDEVAEGKVAKEAIPEVLRKVAGGA
SVEEALKEFVTASQINVEEVVKKVIQQNIEEIKKRGVERSFGLVMGKAMAELRGKVDGKVVAETVKRLLKELGEEGG

Sequences:

>Translated_637_residues
MNLNYEELGLKVGLEIHQQLKTERKLFCHCKPELAEEEEDFDVIERRLRPVRSELGEVDVAALFEWKKGRTYVYRAPVGH
SCLVEADEEPPHEADRDSIIIALAIAKAFNSKIIDEIHVMRKMVIDGSNTSGFQRTMLVALGGELNLGDKKIGIETICVE
EDAARKVEEKEKVTVFNLDRLGIPLIEIATEPTITSPQEAKRVALRIGQMLRLTGRVKRGLGTIRQDLNVSIKGGTKTEI
KGVQKLQIIDKVVEYEVIRQLRLLEIRDELKRRGVKEEEILSQPIKDVTEIFKDTESKLIKRSLSKGSRVYAIKLPKFKG
LLGKEVQPNRRFGTELADYARFWAGVGGIIHSDELPGYGISEDEVEDLKEALGCGEEDAFVMVIAPPPDAKKALEAVKER
AAYALKGVPEETRAANPDGTTRFLRPRPGSARMYPETDVPPIVVTEDMLTKAEKLKPKNPIELIQELITDYKLSKDMAEK
VISSERFDLAYRLIKKYKNKLSANLVARTVVDTLRELEREGLETDKISDEAIEKVLDEVAEGKVAKEAIPEVLRKVAGGA
SVEEALKEFVTASQINVEEVVKKVIQQNIEEIKKRGVERSFGLVMGKAMAELRGKVDGKVVAETVKRLLKELGEEGG
>Mature_637_residues
MNLNYEELGLKVGLEIHQQLKTERKLFCHCKPELAEEEEDFDVIERRLRPVRSELGEVDVAALFEWKKGRTYVYRAPVGH
SCLVEADEEPPHEADRDSIIIALAIAKAFNSKIIDEIHVMRKMVIDGSNTSGFQRTMLVALGGELNLGDKKIGIETICVE
EDAARKVEEKEKVTVFNLDRLGIPLIEIATEPTITSPQEAKRVALRIGQMLRLTGRVKRGLGTIRQDLNVSIKGGTKTEI
KGVQKLQIIDKVVEYEVIRQLRLLEIRDELKRRGVKEEEILSQPIKDVTEIFKDTESKLIKRSLSKGSRVYAIKLPKFKG
LLGKEVQPNRRFGTELADYARFWAGVGGIIHSDELPGYGISEDEVEDLKEALGCGEEDAFVMVIAPPPDAKKALEAVKER
AAYALKGVPEETRAANPDGTTRFLRPRPGSARMYPETDVPPIVVTEDMLTKAEKLKPKNPIELIQELITDYKLSKDMAEK
VISSERFDLAYRLIKKYKNKLSANLVARTVVDTLRELEREGLETDKISDEAIEKVLDEVAEGKVAKEAIPEVLRKVAGGA
SVEEALKEFVTASQINVEEVVKKVIQQNIEEIKKRGVERSFGLVMGKAMAELRGKVDGKVVAETVKRLLKELGEEGG

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319391, Length=283, Percent_Identity=24.3816254416961, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 71332; Mature: 71332

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNYEELGLKVGLEIHQQLKTERKLFCHCKPELAEEEEDFDVIERRLRPVRSELGEVDV
CCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHCCCCCCHHHHHHHHHHHHHHHCCCHH
AALFEWKKGRTYVYRAPVGHSCLVEADEEPPHEADRDSIIIALAIAKAFNSKIIDEIHVM
HHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
RKMVIDGSNTSGFQRTMLVALGGELNLGDKKIGIETICVEEDAARKVEEKEKVTVFNLDR
HHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCEEEEEEHH
LGIPLIEIATEPTITSPQEAKRVALRIGQMLRLTGRVKRGLGTIRQDLNVSIKGGTKTEI
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
KGVQKLQIIDKVVEYEVIRQLRLLEIRDELKRRGVKEEEILSQPIKDVTEIFKDTESKLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRSLSKGSRVYAIKLPKFKGLLGKEVQPNRRFGTELADYARFWAGVGGIIHSDELPGYGI
HHHHCCCCEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
SEDEVEDLKEALGCGEEDAFVMVIAPPPDAKKALEAVKERAAYALKGVPEETRAANPDGT
CHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
TRFLRPRPGSARMYPETDVPPIVVTEDMLTKAEKLKPKNPIELIQELITDYKLSKDMAEK
CEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VISSERFDLAYRLIKKYKNKLSANLVARTVVDTLRELEREGLETDKISDEAIEKVLDEVA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EGKVAKEAIPEVLRKVAGGASVEEALKEFVTASQINVEEVVKKVIQQNIEEIKKRGVERS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
FGLVMGKAMAELRGKVDGKVVAETVKRLLKELGEEGG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLNYEELGLKVGLEIHQQLKTERKLFCHCKPELAEEEEDFDVIERRLRPVRSELGEVDV
CCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHCCCCCCHHHHHHHHHHHHHHHCCCHH
AALFEWKKGRTYVYRAPVGHSCLVEADEEPPHEADRDSIIIALAIAKAFNSKIIDEIHVM
HHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
RKMVIDGSNTSGFQRTMLVALGGELNLGDKKIGIETICVEEDAARKVEEKEKVTVFNLDR
HHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCEEEEEEHH
LGIPLIEIATEPTITSPQEAKRVALRIGQMLRLTGRVKRGLGTIRQDLNVSIKGGTKTEI
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
KGVQKLQIIDKVVEYEVIRQLRLLEIRDELKRRGVKEEEILSQPIKDVTEIFKDTESKLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRSLSKGSRVYAIKLPKFKGLLGKEVQPNRRFGTELADYARFWAGVGGIIHSDELPGYGI
HHHHCCCCEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
SEDEVEDLKEALGCGEEDAFVMVIAPPPDAKKALEAVKERAAYALKGVPEETRAANPDGT
CHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
TRFLRPRPGSARMYPETDVPPIVVTEDMLTKAEKLKPKNPIELIQELITDYKLSKDMAEK
CEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VISSERFDLAYRLIKKYKNKLSANLVARTVVDTLRELEREGLETDKISDEAIEKVLDEVA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EGKVAKEAIPEVLRKVAGGASVEEALKEFVTASQINVEEVVKKVIQQNIEEIKKRGVERS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
FGLVMGKAMAELRGKVDGKVVAETVKRLLKELGEEGG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]