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The map label for this gene is gatB [H]

Identifier: 156936808

GI number: 156936808

Start: 11946

End: 13400

Strand: Reverse

Name: gatB [H]

Synonym: Igni_0013

Alternate gene names: 156936808

Gene position: 13400-11946 (Counterclockwise)

Preceding gene: 156936809

Following gene: 156936805

Centisome position: 1.03

GC content: 52.44

Gene sequence:

>1455_bases
ATGAGGAAAGCGAAGATAGGTCTGGAAGTACACATTCAACTGACCAAACTTAAGACTAAGCTCTTCTGTTCTTGCCCTTC
AGACTATAGGGACAAACCCCCAAACACCGTAGTCTGTCCAGTCTGTCTGGGCCTCCCTGGAGCCCTCCCGAAGCTCAACG
AGGAGGCCGTGAAGATGGCCTTAACTCTCTGTTTGAGCTTAAATGGTAAAGTGAGTCGAAAGGTGGTGTTCGCGAGGAAA
CATTACTTCTACCCGGATTTGCCCAAGGGCTATCAGATTACCCAGTTCACGTCCCTAGGCTCCTCGCCCGTCTGCCAGGG
CGGGCAATTGGTTATGAGGGTTGATGGAAGGGAGAAAGTCGTTCGAATTCGAAGGTTGCAGTTGGAAGAGGACCCCGGAA
GGATAGTTTGGCCTCGTGGGAAGGCGTCGGAGTACGTGCTAGTGGATTACAACAGGTCTGGCATAGCTCTGATAGAGCTG
GTCACCGAGCCAGACATGGAGACGCCCGAGGAGGCGAGAGCTTTCGTAGAGAAGCTGAGGAGCCTCGTGGAACACTTGGG
GATATGTGATTGTGAGCTGGAGGGCGCTATGAGGGTAGACGCCAACGTGAGCGTAGAGGGCGGCGAGAGGGTCGAAATAA
AGAACATAGGCTCTATTAAGGACGTGGAGAAGGCTTTAAAGTTCGAAATAACGCGGCAGAGCACATTGTTAGACCAAGGT
GGGAGGGTGGAGAGGGAGACTAGGCATTGGGACGCGGAGCGGAGGGTGACCGTGGGCGCGCGGAGCAAGGAGTTGGAAGA
AGAATATAGGTATATGCCGGAACCCAACATCCCGCCCTACTTAATTGAGGAGGGACTCCTCGAGCAAGCGAAAAGGGAGC
TCCCCGAGCTCCCAGATCAAAGGATTAAGAGAATGATAAAGGAATACTCCTTGAGCGAGTACCTAGCTTCCGTTCTGGTC
ATAAGGGACAAGAGGTTGGCAGACCTGTTCGAAGAGGCCGCCAAGCTCTACACTAATTACAAGAGGCTAGCGAGCTTGTT
GGTAGTTGACTACCTTAGGTGGGTAGAGGAGCTCGGCCTTCCTTTGGGGAAGTGTGTCAAACCCGAGTGGTTAGCTGAGC
TCCTCTCCTACGTTGACCGAGGAAAGATCACGTTGGAGCAAGCTAGGGAAACGGTGCTGCCTTCCATGCTTAGAGAATGT
AAGGAGCCCTCAAGGGTAATCGAGGAAAAGGGATTAGGTGTAGTTAGGGACGTGGATAAGATACTTAGTATCGTAAGGAA
GGTTTTGAGAGAAAATCCGAAGGCGGTCGAGGATGCGAAGAAGAATCCTAAAGCTATAAACTTCTTAGTCGGACAAGTGA
TGAAGGCCTTGAGAGGGAAGGCGGACCCGCGTACGGTGCGCCAAATGATAGAAAGTGAGCTGGGGATGAGCGCTCGCCAC
GGATCTGACGGATGA

Upstream 100 bases:

>100_bases
TTGGTGCTCCAATACGCTGGCCTACTGGAAAAAGTCATAGAGTGTCTAGAGGGCGAGGGAGAAAAGAGCGAGAAAGGGGA
GAGCGAGGGCGTCGAAGGAC

Downstream 100 bases:

>100_bases
TGACGACCCGTGTTAGCGACCCCACTCATATGTCGACTACGAATTTAACCTTCCAACATTCACCTTCCTTCTTTATCTCC
ATTTCTGCATACGTCATTGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MRKAKIGLEVHIQLTKLKTKLFCSCPSDYRDKPPNTVVCPVCLGLPGALPKLNEEAVKMALTLCLSLNGKVSRKVVFARK
HYFYPDLPKGYQITQFTSLGSSPVCQGGQLVMRVDGREKVVRIRRLQLEEDPGRIVWPRGKASEYVLVDYNRSGIALIEL
VTEPDMETPEEARAFVEKLRSLVEHLGICDCELEGAMRVDANVSVEGGERVEIKNIGSIKDVEKALKFEITRQSTLLDQG
GRVERETRHWDAERRVTVGARSKELEEEYRYMPEPNIPPYLIEEGLLEQAKRELPELPDQRIKRMIKEYSLSEYLASVLV
IRDKRLADLFEEAAKLYTNYKRLASLLVVDYLRWVEELGLPLGKCVKPEWLAELLSYVDRGKITLEQARETVLPSMLREC
KEPSRVIEEKGLGVVRDVDKILSIVRKVLRENPKAVEDAKKNPKAINFLVGQVMKALRGKADPRTVRQMIESELGMSARH
GSDG

Sequences:

>Translated_484_residues
MRKAKIGLEVHIQLTKLKTKLFCSCPSDYRDKPPNTVVCPVCLGLPGALPKLNEEAVKMALTLCLSLNGKVSRKVVFARK
HYFYPDLPKGYQITQFTSLGSSPVCQGGQLVMRVDGREKVVRIRRLQLEEDPGRIVWPRGKASEYVLVDYNRSGIALIEL
VTEPDMETPEEARAFVEKLRSLVEHLGICDCELEGAMRVDANVSVEGGERVEIKNIGSIKDVEKALKFEITRQSTLLDQG
GRVERETRHWDAERRVTVGARSKELEEEYRYMPEPNIPPYLIEEGLLEQAKRELPELPDQRIKRMIKEYSLSEYLASVLV
IRDKRLADLFEEAAKLYTNYKRLASLLVVDYLRWVEELGLPLGKCVKPEWLAELLSYVDRGKITLEQARETVLPSMLREC
KEPSRVIEEKGLGVVRDVDKILSIVRKVLRENPKAVEDAKKNPKAINFLVGQVMKALRGKADPRTVRQMIESELGMSARH
GSDG
>Mature_484_residues
MRKAKIGLEVHIQLTKLKTKLFCSCPSDYRDKPPNTVVCPVCLGLPGALPKLNEEAVKMALTLCLSLNGKVSRKVVFARK
HYFYPDLPKGYQITQFTSLGSSPVCQGGQLVMRVDGREKVVRIRRLQLEEDPGRIVWPRGKASEYVLVDYNRSGIALIEL
VTEPDMETPEEARAFVEKLRSLVEHLGICDCELEGAMRVDANVSVEGGERVEIKNIGSIKDVEKALKFEITRQSTLLDQG
GRVERETRHWDAERRVTVGARSKELEEEYRYMPEPNIPPYLIEEGLLEQAKRELPELPDQRIKRMIKEYSLSEYLASVLV
IRDKRLADLFEEAAKLYTNYKRLASLLVVDYLRWVEELGLPLGKCVKPEWLAELLSYVDRGKITLEQARETVLPSMLREC
KEPSRVIEEKGLGVVRDVDKILSIVRKVLRENPKAVEDAKKNPKAINFLVGQVMKALRGKADPRTVRQMIESELGMSARH
GSDG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=503, Percent_Identity=33.9960238568588, Blast_Score=258, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI115534094, Length=302, Percent_Identity=44.0397350993377, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=503, Percent_Identity=32.0079522862823, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=34.8088531187123, Blast_Score=251, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54980; Mature: 54980

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAKIGLEVHIQLTKLKTKLFCSCPSDYRDKPPNTVVCPVCLGLPGALPKLNEEAVKMA
CCCCCCCEEEEEEEEHHHHHHEECCCHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHH
LTLCLSLNGKVSRKVVFARKHYFYPDLPKGYQITQFTSLGSSPVCQGGQLVMRVDGREKV
HHHHHHCCCCHHHHEEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHHH
VRIRRLQLEEDPGRIVWPRGKASEYVLVDYNRSGIALIELVTEPDMETPEEARAFVEKLR
HHHHHHHCCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHH
SLVEHLGICDCELEGAMRVDANVSVEGGERVEIKNIGSIKDVEKALKFEITRQSTLLDQG
HHHHHCCCCCEECCCCEEECCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
GRVERETRHWDAERRVTVGARSKELEEEYRYMPEPNIPPYLIEEGLLEQAKRELPELPDQ
CCCCHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
RIKRMIKEYSLSEYLASVLVIRDKRLADLFEEAAKLYTNYKRLASLLVVDYLRWVEELGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLGKCVKPEWLAELLSYVDRGKITLEQARETVLPSMLRECKEPSRVIEEKGLGVVRDVDK
CHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHH
ILSIVRKVLRENPKAVEDAKKNPKAINFLVGQVMKALRGKADPRTVRQMIESELGMSARH
HHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
GSDG
CCCC
>Mature Secondary Structure
MRKAKIGLEVHIQLTKLKTKLFCSCPSDYRDKPPNTVVCPVCLGLPGALPKLNEEAVKMA
CCCCCCCEEEEEEEEHHHHHHEECCCHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHH
LTLCLSLNGKVSRKVVFARKHYFYPDLPKGYQITQFTSLGSSPVCQGGQLVMRVDGREKV
HHHHHHCCCCHHHHEEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHHH
VRIRRLQLEEDPGRIVWPRGKASEYVLVDYNRSGIALIELVTEPDMETPEEARAFVEKLR
HHHHHHHCCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHH
SLVEHLGICDCELEGAMRVDANVSVEGGERVEIKNIGSIKDVEKALKFEITRQSTLLDQG
HHHHHCCCCCEECCCCEEECCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
GRVERETRHWDAERRVTVGARSKELEEEYRYMPEPNIPPYLIEEGLLEQAKRELPELPDQ
CCCCHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
RIKRMIKEYSLSEYLASVLVIRDKRLADLFEEAAKLYTNYKRLASLLVVDYLRWVEELGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLGKCVKPEWLAELLSYVDRGKITLEQARETVLPSMLRECKEPSRVIEEKGLGVVRDVDK
CHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHH
ILSIVRKVLRENPKAVEDAKKNPKAINFLVGQVMKALRGKADPRTVRQMIESELGMSARH
HHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
GSDG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]