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The map label for this gene is topA [H]

Identifier: 156936802

GI number: 156936802

Start: 5710

End: 7683

Strand: Reverse

Name: topA [H]

Synonym: Igni_0007

Alternate gene names: 156936802

Gene position: 7683-5710 (Counterclockwise)

Preceding gene: 156936804

Following gene: 156936801

Centisome position: 0.59

GC content: 52.79

Gene sequence:

>1974_bases
ATGAAAGGCCATATACTAGTAATCGCAGAGAAGCCTAAGGCGGCCGAGAAAATAGCGAACGCTCTCGGCAAACCTATAGT
CAAAAGGAAATATGGCGTCCCTTATTGGGAACTAACAAACGGCAAGAAGATAGTCGTGGTACCGGCGGCCGGACACCTGT
TCGGCCTAGCCCCTCTGGGCAAGGGCTTCCCCGTCTTCGACTACGAATGGCGTCCTCTTTACGAAATCGAGAAGGGAGCC
TCCCACACGAAGAAATACATAGCTTTGATCAGCAGCCTCAGTCCCGGTTCGAGCCTCTACGTTAACGCGTGTGACTATGA
CGTGGAAGGTTCCGTTATAGGGTATATAATAATAGAAAGGTTCGGGGACCCCCGCCGCAGCAAGAGGATGGTTTTCCACG
CCTTAACTCCGGACGAGATACGTAGGGCGTGGAACAGCCTGAGGCCGCTCGACTGGGAGATGATAAACGCGGGGTTGGCG
AGACATGAGCTGGACTGGATCTGGGGGATCAACGTCTCTCGAGCTCTGATGCACGCTTACAGGGTGGCCACCGGCAAGAG
CAAGAGTTTGTCAGCCGGAAGGGTTCAGAGCCCCACCTTAAAGGAGGTGGTGGAGAGGGAGCTCGCCGTCCAGTGCTTCG
TCCCGGAGCCCTATTTTTCGGTCTCACTGAGCGCTAAGGGAGTCTCCCTCGAACCCACACTTAAGTTCGAAACAAAGGAC
GAGGCAGACCACTTTATTTCAAACTGCCTCGGAAAAGTAGTTGACGTCATCGTAAATGACGAAAGGCTATCACCTCCACA
CCCCTTCAACTTGCCGGACCTCCAAATGGAGGCCGCGAGGCTCTACGGCTTCTCGCCTTCGAAGACTCAGAGCATCGCCG
AAGAGCTTTACCTCGACGCCTACATTTCCTACCCGAGGACGAATAGCCAAAAATATCCCAAGGATTTGGATTACGCGAAC
ATACTAAAGGGCATTGCCTCAGTTCCTCAATACCGCCCCTTGACCAAGGCCCTCTTCAAGGAAACCAGGGGACGTTTCTC
TCCGAACAACGGACCCAAGGACGACCCGGCCCACCCGGCGATCTACCCTACGGGGAAGCCCCCGAAGAACTTGAGTCCAG
ACCACATGAAGGTATACGACTTAATAGTGAGGAGGTTCATGGCAACCCTCGCTTCCGAAAGCGTTTATGAAGTAACCAAA
GTGGTGATAGAGGTTAACGGTCAGACCTTCAAAGCCGTGGGTAGGAGGGTCAAGTGGAAGGGTTGGACGCTATACTATCC
CTTCTCGGCTCCTCAAGAAAAGAGCTTGCCGAGCTTTACTAAAGGGGAGAGCCTCCAAGTCAAGTGCACCAGGAGGTTGC
AGTACACAAAGCCCCCAGAGCGGTACACCAAGGCGAAGATAGTCAAGTGGATGGAAAGGGTTGGAATAGGGACCGAGGCG
ACTAGGGCCAGAATAGTGGAGACCTTGTTCAAGAGGGGGTATCTAAAGAATGTTGGTTCGAAAGTATACGCGACCGACCT
GGGGATAGGCGTGGCGTTGGTGCTCAGCAAATTCTTTCCCGAAATAGTTAGCGTTGAACTAACTAGGAGGTTCGAAGAAG
ATATGAAAAAGATTATGATGGGCGTGGAACGTAAGGAAAAGATAGTTGAGGAGGCTAAGAAGTTCATGAGAATGATATTG
GCCAAGTATAAACCTCACATGAGAGAGGTGGGCGAATACCTTGCTTACTCTCTGGGTGAAATGGAGCCTCCGGGGAGGTG
TGCGGTTTGTTGGAGGGTGGCCGAGGAGGACGGGCTCTGCGTCGTACACAAGAGAGCTAGGGACGAGCTCTACGCGAAGT
ACCCCGTCTGGAGGAGGAGGTTGGGGTTAAGCTTTGAAGAGTACCTAAAGAAGGTAACGACTTTAAAGCAAGCGGGGACG
GCGGCCAAGGAGGTCGCAAAGGCGTTACTGTCGGAACCTACGTTTAAAGGTTAA

Upstream 100 bases:

>100_bases
TATCGTATATGTGGCCGGGGAGTTCTACGATCCACTTCCTCTTCAAAGCTTGCGGCCCGTCGAGTTATAATCAAAGGGCT
AATACAGTTGCGGAGAACGC

Downstream 100 bases:

>100_bases
ATAATTGCGATGCGAAGGACGCTTCAGGGAGCGCGCTTTTGAGTAGCGGGTTAACGTTCCAAGGCAGAGCGGTTCCGGAG
GAAGGTCAAGCCAGCGGCGA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MKGHILVIAEKPKAAEKIANALGKPIVKRKYGVPYWELTNGKKIVVVPAAGHLFGLAPLGKGFPVFDYEWRPLYEIEKGA
SHTKKYIALISSLSPGSSLYVNACDYDVEGSVIGYIIIERFGDPRRSKRMVFHALTPDEIRRAWNSLRPLDWEMINAGLA
RHELDWIWGINVSRALMHAYRVATGKSKSLSAGRVQSPTLKEVVERELAVQCFVPEPYFSVSLSAKGVSLEPTLKFETKD
EADHFISNCLGKVVDVIVNDERLSPPHPFNLPDLQMEAARLYGFSPSKTQSIAEELYLDAYISYPRTNSQKYPKDLDYAN
ILKGIASVPQYRPLTKALFKETRGRFSPNNGPKDDPAHPAIYPTGKPPKNLSPDHMKVYDLIVRRFMATLASESVYEVTK
VVIEVNGQTFKAVGRRVKWKGWTLYYPFSAPQEKSLPSFTKGESLQVKCTRRLQYTKPPERYTKAKIVKWMERVGIGTEA
TRARIVETLFKRGYLKNVGSKVYATDLGIGVALVLSKFFPEIVSVELTRRFEEDMKKIMMGVERKEKIVEEAKKFMRMIL
AKYKPHMREVGEYLAYSLGEMEPPGRCAVCWRVAEEDGLCVVHKRARDELYAKYPVWRRRLGLSFEEYLKKVTTLKQAGT
AAKEVAKALLSEPTFKG

Sequences:

>Translated_657_residues
MKGHILVIAEKPKAAEKIANALGKPIVKRKYGVPYWELTNGKKIVVVPAAGHLFGLAPLGKGFPVFDYEWRPLYEIEKGA
SHTKKYIALISSLSPGSSLYVNACDYDVEGSVIGYIIIERFGDPRRSKRMVFHALTPDEIRRAWNSLRPLDWEMINAGLA
RHELDWIWGINVSRALMHAYRVATGKSKSLSAGRVQSPTLKEVVERELAVQCFVPEPYFSVSLSAKGVSLEPTLKFETKD
EADHFISNCLGKVVDVIVNDERLSPPHPFNLPDLQMEAARLYGFSPSKTQSIAEELYLDAYISYPRTNSQKYPKDLDYAN
ILKGIASVPQYRPLTKALFKETRGRFSPNNGPKDDPAHPAIYPTGKPPKNLSPDHMKVYDLIVRRFMATLASESVYEVTK
VVIEVNGQTFKAVGRRVKWKGWTLYYPFSAPQEKSLPSFTKGESLQVKCTRRLQYTKPPERYTKAKIVKWMERVGIGTEA
TRARIVETLFKRGYLKNVGSKVYATDLGIGVALVLSKFFPEIVSVELTRRFEEDMKKIMMGVERKEKIVEEAKKFMRMIL
AKYKPHMREVGEYLAYSLGEMEPPGRCAVCWRVAEEDGLCVVHKRARDELYAKYPVWRRRLGLSFEEYLKKVTTLKQAGT
AAKEVAKALLSEPTFKG
>Mature_657_residues
MKGHILVIAEKPKAAEKIANALGKPIVKRKYGVPYWELTNGKKIVVVPAAGHLFGLAPLGKGFPVFDYEWRPLYEIEKGA
SHTKKYIALISSLSPGSSLYVNACDYDVEGSVIGYIIIERFGDPRRSKRMVFHALTPDEIRRAWNSLRPLDWEMINAGLA
RHELDWIWGINVSRALMHAYRVATGKSKSLSAGRVQSPTLKEVVERELAVQCFVPEPYFSVSLSAKGVSLEPTLKFETKD
EADHFISNCLGKVVDVIVNDERLSPPHPFNLPDLQMEAARLYGFSPSKTQSIAEELYLDAYISYPRTNSQKYPKDLDYAN
ILKGIASVPQYRPLTKALFKETRGRFSPNNGPKDDPAHPAIYPTGKPPKNLSPDHMKVYDLIVRRFMATLASESVYEVTK
VVIEVNGQTFKAVGRRVKWKGWTLYYPFSAPQEKSLPSFTKGESLQVKCTRRLQYTKPPERYTKAKIVKWMERVGIGTEA
TRARIVETLFKRGYLKNVGSKVYATDLGIGVALVLSKFFPEIVSVELTRRFEEDMKKIMMGVERKEKIVEEAKKFMRMIL
AKYKPHMREVGEYLAYSLGEMEPPGRCAVCWRVAEEDGLCVVHKRARDELYAKYPVWRRRLGLSFEEYLKKVTTLKQAGT
AAKEVAKALLSEPTFKG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic type I/III topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=596, Percent_Identity=27.3489932885906, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4507635, Length=642, Percent_Identity=25.0778816199377, Blast_Score=143, Evalue=6e-34,
Organism=Escherichia coli, GI1787529, Length=494, Percent_Identity=29.1497975708502, Blast_Score=185, Evalue=9e-48,
Organism=Escherichia coli, GI1788061, Length=569, Percent_Identity=28.9982425307557, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32563869, Length=639, Percent_Identity=27.3865414710485, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17555378, Length=471, Percent_Identity=27.1762208067941, Blast_Score=140, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323263, Length=636, Percent_Identity=24.685534591195, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24585251, Length=610, Percent_Identity=27.0491803278689, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24640096, Length=631, Percent_Identity=24.405705229794, Blast_Score=144, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74507; Mature: 74507

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGHILVIAEKPKAAEKIANALGKPIVKRKYGVPYWELTNGKKIVVVPAAGHLFGLAPLG
CCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEECCCCCEEECCCC
KGFPVFDYEWRPLYEIEKGASHTKKYIALISSLSPGSSLYVNACDYDVEGSVIGYIIIER
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEEC
FGDPRRSKRMVFHALTPDEIRRAWNSLRPLDWEMINAGLARHELDWIWGINVSRALMHAY
CCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHH
RVATGKSKSLSAGRVQSPTLKEVVERELAVQCFVPEPYFSVSLSAKGVSLEPTLKFETKD
HHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCEEEEEECCCCEECCCEEECCCC
EADHFISNCLGKVVDVIVNDERLSPPHPFNLPDLQMEAARLYGFSPSKTQSIAEELYLDA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
YISYPRTNSQKYPKDLDYANILKGIASVPQYRPLTKALFKETRGRFSPNNGPKDDPAHPA
HCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IYPTGKPPKNLSPDHMKVYDLIVRRFMATLASESVYEVTKVVIEVNGQTFKAVGRRVKWK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHCCEEEEC
GWTLYYPFSAPQEKSLPSFTKGESLQVKCTRRLQYTKPPERYTKAKIVKWMERVGIGTEA
CEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHH
TRARIVETLFKRGYLKNVGSKVYATDLGIGVALVLSKFFPEIVSVELTRRFEEDMKKIMM
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVERKEKIVEEAKKFMRMILAKYKPHMREVGEYLAYSLGEMEPPGRCAVCWRVAEEDGLC
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEE
VVHKRARDELYAKYPVWRRRLGLSFEEYLKKVTTLKQAGTAAKEVAKALLSEPTFKG
EEEHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGHILVIAEKPKAAEKIANALGKPIVKRKYGVPYWELTNGKKIVVVPAAGHLFGLAPLG
CCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEECCCCCEEECCCC
KGFPVFDYEWRPLYEIEKGASHTKKYIALISSLSPGSSLYVNACDYDVEGSVIGYIIIER
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEEC
FGDPRRSKRMVFHALTPDEIRRAWNSLRPLDWEMINAGLARHELDWIWGINVSRALMHAY
CCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHH
RVATGKSKSLSAGRVQSPTLKEVVERELAVQCFVPEPYFSVSLSAKGVSLEPTLKFETKD
HHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCEEEEEECCCCEECCCEEECCCC
EADHFISNCLGKVVDVIVNDERLSPPHPFNLPDLQMEAARLYGFSPSKTQSIAEELYLDA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
YISYPRTNSQKYPKDLDYANILKGIASVPQYRPLTKALFKETRGRFSPNNGPKDDPAHPA
HCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IYPTGKPPKNLSPDHMKVYDLIVRRFMATLASESVYEVTKVVIEVNGQTFKAVGRRVKWK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHCCEEEEC
GWTLYYPFSAPQEKSLPSFTKGESLQVKCTRRLQYTKPPERYTKAKIVKWMERVGIGTEA
CEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHH
TRARIVETLFKRGYLKNVGSKVYATDLGIGVALVLSKFFPEIVSVELTRRFEEDMKKIMM
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVERKEKIVEEAKKFMRMILAKYKPHMREVGEYLAYSLGEMEPPGRCAVCWRVAEEDGLC
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEE
VVHKRARDELYAKYPVWRRRLGLSFEEYLKKVTTLKQAGTAAKEVAKALLSEPTFKG
EEEHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]