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The map label for this gene is oprF [H]

Identifier: 156936614

GI number: 156936614

Start: 57392

End: 59095

Strand: Direct

Name: oprF [H]

Synonym: ESA_pESA3p05495

Alternate gene names: 156936614

Gene position: 57392-59095 (Clockwise)

Preceding gene: 156936613

Following gene: 156936615

Centisome position: 43.75

GC content: 59.68

Gene sequence:

>1704_bases
ATGCGTGAACGAAACCGCCCGCTTTTTACTCTCTTTGCCACAGTGCTGGCGCTATGGCTGGTGCTGGGGTTCTGGCCCTT
TTCTGCGTTGAATAAGCTTTTACTGAGTGTCGGTATCCTTCTGACAGGCGGGGCAGCGCTGTGGTACCTGCGGCGTTATA
GTTTGCGTCGTCAGGCTTCGTGCGCCCAGGTGGCAGCCGCTCTTTTGCCGCCGGAAAATTTCCAGGGGGCGCTGGTGTTG
GTCGGCGGTGACACAGGAGCACTCTTTCCGGCAGGGGGTGATTATCGGGAAAGCCGACAGGGCTGGTATCTTCGCGCGGC
AAATGCAGAGCAGCTCCCTTTACTGGCGCAGCATATTGCGCTCACCCGGCCTGCTCTGGTGTCGCAATTGTCGATTCTGT
TGGCTGTCGTGCCTGAGCAGCAGGTCTGCGATGACACGCTGACGCAGTCGCTGCGGGTATGGCACAGAAGTATTCTGGCG
TGCCGTAACTGGCTTAATGGCTTGCCGCCCGTATGGAGCGTGGTCTGGCTCAACACGCTTGATGCCGGTGGTAACACCCT
GTGGTTTACCCTTACACCGGGGCGTCCGGGTTTACAGGCCCGGCAGGCTGACCATGTGACATTACCGATAGCAGAGTGGC
AGCGGGAGCCCGCTTTCAGCCCATCACGTTTATCTCCGGTACTCTGGCTGGATAGCGTGATGGCTTTTATTCACCGGTAT
ATCTGTCAGCCACTCGCCAGCCCTCAAAATGAGCTTCCTGCGCTGCCTCTCGCTGCAGTGGGTGTTTGCATGACGTCTGT
TGAATCAGTGGTTGATAACCTCTGGCATAAGCAGATTGCCAGCATGACCACACTCCCGCCTTTGACACAGAAATCCAGTG
AACCATTGCCATTGCCCGATATGTTGCTGGCGTCACTGCCTTGCTGGCGAGGCATTAGCTGCAGAATGCGGGATCTCCGC
ATCGCGGGGGTTATCACCTTCTTGTTTATCGGGCTGGCGTTGTGTGCCTCGTTTATGAATAACCAGCGGCTGGTACGTCG
TATTGGCGACCATCTTGCGCGTTATCACCATCTCAGCGGGATGCCACCCGCACCAAAAGCGCAGGCACAGCAGCGCCTGC
GTACTGACAGTCAGCTGCTTGATGACTGGCTGAGGCGCGGCGAACCGCTGCGTTACGGGCTTGGACTCTACCAGGGCGAG
AAACTGATACCGCCATTAGACGCGGCCATCAGTGACTGGTCAGCGCCTGTCTCATCACCTCCGGTTAACAAGAACATCAT
TACCGAACCACAGACGATCCGCCTGGACGCGCTTTCGCTGTTCGACACCGGTCAGTGGCGGCTGAAAACCGGCTCCACGA
AAGTGCTGGTGAACGCGCTGGTGGGCATTAAGGCCAGGCCGGGCTGGCTGATTGTGGTCGCCGGGCATACCGACGATGTG
GGCGACGAACGCGCCAACCAGCAGCTGTCGCTTAAGCGCGCCGAAGCGGTGCGCGACTGGATGCGCGGCACCGGCGACGT
GCCGGAGAGCTGCTTTGCGGTGCAGGGCTACGGCGAAAGCCGCCCGGCGGTGCCCAACACCACGGCAGAAGGGCGGGCGC
TGAACCGTCGCGTTGAAATCAGTCTGGTGCCGCAGGCGGACGCCTGCCGGCTGCCGGACATTCCCCTGCCGCCCTCACAG
GATGAGAGCGGTACTCAACCTTAA

Upstream 100 bases:

>100_bases
GGGGTGGATCGCGGGGATAGTGACGCTGGCGGCGCTGTGGCTTGCGTTTTCCGCTGCGCTGACGCGCCAGGTTGCGCAAA
TAGCAGGGCAGGGATGACGG

Downstream 100 bases:

>100_bases
ATGGAGACAATCCCATGGCAATTCCTGTTTATCTCTGGCTGAAGGACGACGGTGGCGCGGACATCAAGGGTTCCGTTGAT
GTGAAGGACCGCGAGGGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MRERNRPLFTLFATVLALWLVLGFWPFSALNKLLLSVGILLTGGAALWYLRRYSLRRQASCAQVAAALLPPENFQGALVL
VGGDTGALFPAGGDYRESRQGWYLRAANAEQLPLLAQHIALTRPALVSQLSILLAVVPEQQVCDDTLTQSLRVWHRSILA
CRNWLNGLPPVWSVVWLNTLDAGGNTLWFTLTPGRPGLQARQADHVTLPIAEWQREPAFSPSRLSPVLWLDSVMAFIHRY
ICQPLASPQNELPALPLAAVGVCMTSVESVVDNLWHKQIASMTTLPPLTQKSSEPLPLPDMLLASLPCWRGISCRMRDLR
IAGVITFLFIGLALCASFMNNQRLVRRIGDHLARYHHLSGMPPAPKAQAQQRLRTDSQLLDDWLRRGEPLRYGLGLYQGE
KLIPPLDAAISDWSAPVSSPPVNKNIITEPQTIRLDALSLFDTGQWRLKTGSTKVLVNALVGIKARPGWLIVVAGHTDDV
GDERANQQLSLKRAEAVRDWMRGTGDVPESCFAVQGYGESRPAVPNTTAEGRALNRRVEISLVPQADACRLPDIPLPPSQ
DESGTQP

Sequences:

>Translated_567_residues
MRERNRPLFTLFATVLALWLVLGFWPFSALNKLLLSVGILLTGGAALWYLRRYSLRRQASCAQVAAALLPPENFQGALVL
VGGDTGALFPAGGDYRESRQGWYLRAANAEQLPLLAQHIALTRPALVSQLSILLAVVPEQQVCDDTLTQSLRVWHRSILA
CRNWLNGLPPVWSVVWLNTLDAGGNTLWFTLTPGRPGLQARQADHVTLPIAEWQREPAFSPSRLSPVLWLDSVMAFIHRY
ICQPLASPQNELPALPLAAVGVCMTSVESVVDNLWHKQIASMTTLPPLTQKSSEPLPLPDMLLASLPCWRGISCRMRDLR
IAGVITFLFIGLALCASFMNNQRLVRRIGDHLARYHHLSGMPPAPKAQAQQRLRTDSQLLDDWLRRGEPLRYGLGLYQGE
KLIPPLDAAISDWSAPVSSPPVNKNIITEPQTIRLDALSLFDTGQWRLKTGSTKVLVNALVGIKARPGWLIVVAGHTDDV
GDERANQQLSLKRAEAVRDWMRGTGDVPESCFAVQGYGESRPAVPNTTAEGRALNRRVEISLVPQADACRLPDIPLPPSQ
DESGTQP
>Mature_567_residues
MRERNRPLFTLFATVLALWLVLGFWPFSALNKLLLSVGILLTGGAALWYLRRYSLRRQASCAQVAAALLPPENFQGALVL
VGGDTGALFPAGGDYRESRQGWYLRAANAEQLPLLAQHIALTRPALVSQLSILLAVVPEQQVCDDTLTQSLRVWHRSILA
CRNWLNGLPPVWSVVWLNTLDAGGNTLWFTLTPGRPGLQARQADHVTLPIAEWQREPAFSPSRLSPVLWLDSVMAFIHRY
ICQPLASPQNELPALPLAAVGVCMTSVESVVDNLWHKQIASMTTLPPLTQKSSEPLPLPDMLLASLPCWRGISCRMRDLR
IAGVITFLFIGLALCASFMNNQRLVRRIGDHLARYHHLSGMPPAPKAQAQQRLRTDSQLLDDWLRRGEPLRYGLGLYQGE
KLIPPLDAAISDWSAPVSSPPVNKNIITEPQTIRLDALSLFDTGQWRLKTGSTKVLVNALVGIKARPGWLIVVAGHTDDV
GDERANQQLSLKRAEAVRDWMRGTGDVPESCFAVQGYGESRPAVPNTTAEGRALNRRVEISLVPQADACRLPDIPLPPSQ
DESGTQP

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=70, Percent_Identity=47.1428571428571, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI48994946, Length=104, Percent_Identity=37.5, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 62531; Mature: 62531

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERNRPLFTLFATVLALWLVLGFWPFSALNKLLLSVGILLTGGAALWYLRRYSLRRQAS
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
CAQVAAALLPPENFQGALVLVGGDTGALFPAGGDYRESRQGWYLRAANAEQLPLLAQHIA
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
LTRPALVSQLSILLAVVPEQQVCDDTLTQSLRVWHRSILACRNWLNGLPPVWSVVWLNTL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
DAGGNTLWFTLTPGRPGLQARQADHVTLPIAEWQREPAFSPSRLSPVLWLDSVMAFIHRY
CCCCCEEEEEECCCCCCCCCCCCCCEEEEHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHH
ICQPLASPQNELPALPLAAVGVCMTSVESVVDNLWHKQIASMTTLPPLTQKSSEPLPLPD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
MLLASLPCWRGISCRMRDLRIAGVITFLFIGLALCASFMNNQRLVRRIGDHLARYHHLSG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
MPPAPKAQAQQRLRTDSQLLDDWLRRGEPLRYGLGLYQGEKLIPPLDAAISDWSAPVSSP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHCCCCCCCCHHHHHHHCCCCCCCC
PVNKNIITEPQTIRLDALSLFDTGQWRLKTGSTKVLVNALVGIKARPGWLIVVAGHTDDV
CCCCCCCCCCCEEEEEHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCC
GDERANQQLSLKRAEAVRDWMRGTGDVPESCFAVQGYGESRPAVPNTTAEGRALNRRVEI
CHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCCCCCCCCHHCCEEEE
SLVPQADACRLPDIPLPPSQDESGTQP
EECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRERNRPLFTLFATVLALWLVLGFWPFSALNKLLLSVGILLTGGAALWYLRRYSLRRQAS
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
CAQVAAALLPPENFQGALVLVGGDTGALFPAGGDYRESRQGWYLRAANAEQLPLLAQHIA
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
LTRPALVSQLSILLAVVPEQQVCDDTLTQSLRVWHRSILACRNWLNGLPPVWSVVWLNTL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
DAGGNTLWFTLTPGRPGLQARQADHVTLPIAEWQREPAFSPSRLSPVLWLDSVMAFIHRY
CCCCCEEEEEECCCCCCCCCCCCCCEEEEHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHH
ICQPLASPQNELPALPLAAVGVCMTSVESVVDNLWHKQIASMTTLPPLTQKSSEPLPLPD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
MLLASLPCWRGISCRMRDLRIAGVITFLFIGLALCASFMNNQRLVRRIGDHLARYHHLSG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
MPPAPKAQAQQRLRTDSQLLDDWLRRGEPLRYGLGLYQGEKLIPPLDAAISDWSAPVSSP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHCCCCCCCCHHHHHHHCCCCCCCC
PVNKNIITEPQTIRLDALSLFDTGQWRLKTGSTKVLVNALVGIKARPGWLIVVAGHTDDV
CCCCCCCCCCCEEEEEHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCC
GDERANQQLSLKRAEAVRDWMRGTGDVPESCFAVQGYGESRPAVPNTTAEGRALNRRVEI
CHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCCCCCCCCHHCCEEEE
SLVPQADACRLPDIPLPPSQDESGTQP
EECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]