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Identifier: 15679679

GI number: 15679679

Start: 1544148

End: 1545317

Strand: Direct

Name: Not Available

Synonym: MTH1685

Alternate gene names: 15679679

Gene position: 1544148-1545317 (Clockwise)

Preceding gene: 15679678

Following gene: 15679680

Centisome position: 88.17

GC content: 51.97

Gene sequence:

>1170_bases
ATGATTCTTGATTATGATGTTATACTTGCACGCTACGGTGAGGTCGCCATCAAGGGCCCCTCAGTGAGGAGAAGATTCGA
AGGAAAACTTCTCCATAACATAAAGTCGGCCTTCAGCTGCAGAGCAGAGCTCAGACATGGAAGGATATTCATTTTTCCTG
AAGACATGGATGAAGCCCTTGATAGACTCTCAAAGATATTTGGTATCGTGTCATTTTCTCCGGCCGTAACAGCTGAGACG
GGTTTTGATAGCATAGAGGATTCACTGAGGGAATACATCCATGAGCTGAGATCTGAAGGTCTCCTCACCAGCAGAACACC
CTTCGCCATAAGGTGCCGCAGGGTTGGTGAGCATGATTTCACGAGCCAGGAGATGGCGGCATTTGCTGGGTCAGTTGTTG
TGGGGGAGATCGGTGCTCCGGTTGACCTCGGAAACCCTGACCTGGAGATTCACCTCGAGATAAGGGAGGATGAGACCTAC
ATCTACCACAGGGTAATACCAGGACCAGGGGGTCTTCCTGCAGGGACTCAGGGAAAGGTTGTGGCACTCCTATCAGGGGG
CATTGACTCGCCGGTGGCCACCTACCTGATGATGAAGAGGGGATGTCAGGTAGTGGCGGTCCATATGGACAATGCGCCCT
TCACAGGTGAGGAAGCAGAGGAGAAGGTTGAAAAGATCGCTGCAAAGCTCGCAGAATACTCTGCAGGTGTTGAATTCAAA
CTGAGGACCTTCAGTTATGGAAGATATCTTGAAAGCTGCCGTAGAGGGGCTCCCGAGAAGATGACTTGCGTGCTCTGCAA
ATTCGGGATGTACCATCTTGCAGAGATGGTTGCAGAGGAGGAGGGGGCCCTTGCAATAGTCGATGGGAGCAGCCTTGGTC
AGGTGGCATCCCAGACCCTCCCGAACATCCTTGCCACAAGGATGGGTGTGAATATTCCCATTCTGAGCCCCCTGATAGGC
ATGGATAAGGTGGAGATAGAGAACCTTGCAAAGAGGATAGGCACCTACGATATCTCCGTGATACCAGATGGGGGCTGTAG
CGCCGTGCCGGCCCACCCATCAACAGCCTCGCCTCCAGAGGCAGTCATGGAGGCATCTGAGAAGATCAATGTTAAGGAAG
AAGTTGCGGAGATCTTCAGGAAGGGCTCAAAGACGAGGATATTCAGCTGA

Upstream 100 bases:

>100_bases
CCGCGAACTATCCCTGGAACTCAAAAAGCTCATAGAAAACGGGGATTTCCTCATGGGATGAGCGAATGGTTTTGGATGAG
GTATAGATGATGGGGGTGTT

Downstream 100 bases:

>100_bases
TCCACGGAGTAGGGGTGGGTGGATAACTTTTTAAATAATCAGATCCACATTTTATATAAGATCCATTTCATCAACTCACT
ATCTTCTGGAGGTGTAATAC

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MILDYDVILARYGEVAIKGPSVRRRFEGKLLHNIKSAFSCRAELRHGRIFIFPEDMDEALDRLSKIFGIVSFSPAVTAET
GFDSIEDSLREYIHELRSEGLLTSRTPFAIRCRRVGEHDFTSQEMAAFAGSVVVGEIGAPVDLGNPDLEIHLEIREDETY
IYHRVIPGPGGLPAGTQGKVVALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKLAEYSAGVEFK
LRTFSYGRYLESCRRGAPEKMTCVLCKFGMYHLAEMVAEEEGALAIVDGSSLGQVASQTLPNILATRMGVNIPILSPLIG
MDKVEIENLAKRIGTYDISVIPDGGCSAVPAHPSTASPPEAVMEASEKINVKEEVAEIFRKGSKTRIFS

Sequences:

>Translated_389_residues
MILDYDVILARYGEVAIKGPSVRRRFEGKLLHNIKSAFSCRAELRHGRIFIFPEDMDEALDRLSKIFGIVSFSPAVTAET
GFDSIEDSLREYIHELRSEGLLTSRTPFAIRCRRVGEHDFTSQEMAAFAGSVVVGEIGAPVDLGNPDLEIHLEIREDETY
IYHRVIPGPGGLPAGTQGKVVALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKLAEYSAGVEFK
LRTFSYGRYLESCRRGAPEKMTCVLCKFGMYHLAEMVAEEEGALAIVDGSSLGQVASQTLPNILATRMGVNIPILSPLIG
MDKVEIENLAKRIGTYDISVIPDGGCSAVPAHPSTASPPEAVMEASEKINVKEEVAEIFRKGSKTRIFS
>Mature_389_residues
MILDYDVILARYGEVAIKGPSVRRRFEGKLLHNIKSAFSCRAELRHGRIFIFPEDMDEALDRLSKIFGIVSFSPAVTAET
GFDSIEDSLREYIHELRSEGLLTSRTPFAIRCRRVGEHDFTSQEMAAFAGSVVVGEIGAPVDLGNPDLEIHLEIREDETY
IYHRVIPGPGGLPAGTQGKVVALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKLAEYSAGVEFK
LRTFSYGRYLESCRRGAPEKMTCVLCKFGMYHLAEMVAEEEGALAIVDGSSLGQVASQTLPNILATRMGVNIPILSPLIG
MDKVEIENLAKRIGTYDISVIPDGGCSAVPAHPSTASPPEAVMEASEKINVKEEVAEIFRKGSKTRIFS

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=377, Percent_Identity=32.3607427055703, Blast_Score=147, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_METTH (O27720)

Other databases:

- EMBL:   AE000666
- PIR:   C69092
- RefSeq:   NP_276796.1
- ProteinModelPortal:   O27720
- SMR:   O27720
- STRING:   O27720
- GeneID:   1470770
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1685
- NMPDR:   fig|187420.1.peg.1651
- eggNOG:   arNOG06091
- HOGENOM:   HBG646072
- OMA:   RREWGRI
- ProtClustDB:   CLSK2392172
- BioCyc:   MTHE187420:MTH1685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 42473; Mature: 42473

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS51165 THUMP

Important sites: BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDYDVILARYGEVAIKGPSVRRRFEGKLLHNIKSAFSCRAELRHGRIFIFPEDMDEAL
CEECHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
DRLSKIFGIVSFSPAVTAETGFDSIEDSLREYIHELRSEGLLTSRTPFAIRCRRVGEHDF
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCC
TSQEMAAFAGSVVVGEIGAPVDLGNPDLEIHLEIREDETYIYHRVIPGPGGLPAGTQGKV
CHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCE
VALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKLAEYSAGVEFK
EEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LRTFSYGRYLESCRRGAPEKMTCVLCKFGMYHLAEMVAEEEGALAIVDGSSLGQVASQTL
EEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
PNILATRMGVNIPILSPLIGMDKVEIENLAKRIGTYDISVIPDGGCSAVPAHPSTASPPE
HHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHH
AVMEASEKINVKEEVAEIFRKGSKTRIFS
HHHHHHHCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MILDYDVILARYGEVAIKGPSVRRRFEGKLLHNIKSAFSCRAELRHGRIFIFPEDMDEAL
CEECHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
DRLSKIFGIVSFSPAVTAETGFDSIEDSLREYIHELRSEGLLTSRTPFAIRCRRVGEHDF
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCC
TSQEMAAFAGSVVVGEIGAPVDLGNPDLEIHLEIREDETYIYHRVIPGPGGLPAGTQGKV
CHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCE
VALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKLAEYSAGVEFK
EEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LRTFSYGRYLESCRRGAPEKMTCVLCKFGMYHLAEMVAEEEGALAIVDGSSLGQVASQTL
EEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
PNILATRMGVNIPILSPLIGMDKVEIENLAKRIGTYDISVIPDGGCSAVPAHPSTASPPE
HHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHH
AVMEASEKINVKEEVAEIFRKGSKTRIFS
HHHHHHHCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463