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The map label for this gene is trpA

Identifier: 15679655

GI number: 15679655

Start: 1523724

End: 1524545

Strand: Direct

Name: trpA

Synonym: MTH1660

Alternate gene names: 15679655

Gene position: 1523724-1524545 (Clockwise)

Preceding gene: 15679654

Following gene: 15679656

Centisome position: 87.0

GC content: 53.16

Gene sequence:

>822_bases
GTGATTGTAATGTCAGGAGCTAAATCAATGAGTTATGCTGAGATGTTCAGACGGGCGGATGGCTGTGCCTTCGTCCCCTT
CGTGGTTGCAGGGGACCCTGATATGGAGACATCCCTTGAGATCATAAGGACCCTTGTGGATGCAGGGGCCGATGCCCTTG
AGGTAGGCTTCCCATTCTCTGACCCAATAGCAGATGGTACAAGCGTTCAGGGCGCTGATCTGCGGGCCCTCAGGGCAGGC
ATGACAACCGAGAAGTGCTTCCAGCTTATAGAGAGGGTCCGGGAATTCACTTCCATACCCATAGGCCTGCTGGTATACTA
CAACCTGATCTACAGGATGGGTGTTGATGAGTTCTACCGGAGGGCTGCAGAAGCAGGGGTCACAGGGATACTTGCAGCGG
ATCTCCCCCCTGAAGAGGCCTCAGATGCACTCAGGGCCGCTGAAAAATATGATATAGATCAGATATTCATAGTTGCACCA
ACAACCGGTAGTGAACGCCTCAAAAGGATCTCTGAGGTCTCATCAGGATTTCACTACCTCGTCTCGGTGATGGGGGTCAC
CGGCGCAAGATCAAGGGTTGAGGATGCAACCATCGAACTCATAAAAAGGGTGAAGGCTGAGGGAAGTCTGCCGGTAATGG
TTGGATTCGGTGTATCAAGACCCGAACATGTGAGGATGCTGAGGGATGCAGGAGCCGATGGAGTTATCGTCGGAAGCGCC
ATCATAGACGTGATATCCGGGAACCTCGGCGACAGGGAGCTTATGCTCCAGAGGATCCATGAAATGGCAGGCACTCTGAA
AGCCGCAGGAGGATCAGGATGA

Upstream 100 bases:

>100_bases
CTCTCAGGAAGGGGAGATAAGGACATGTTCATGGTTGCTGGTTTGCTGGGGGTGGGTGTGTGATTGCTGATGTGTTCATG
GTTGCTGGTTTGCTGGGAGT

Downstream 100 bases:

>100_bases
AATTCAGGAGGATGATCTCTGAAATAATGGACTTCAGGAACCTCAGTGAGGATGAGGCCTACAGTCTGATGGAGATGATA
ATGGCTGGTGAACTTGATGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIVMSGAKSMSYAEMFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFSDPIADGTSVQGADLRALRAG
MTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYRRAAEAGVTGILAADLPPEEASDALRAAEKYDIDQIFIVAP
TTGSERLKRISEVSSGFHYLVSVMGVTGARSRVEDATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSA
IIDVISGNLGDRELMLQRIHEMAGTLKAAGGSG

Sequences:

>Translated_273_residues
MIVMSGAKSMSYAEMFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFSDPIADGTSVQGADLRALRAG
MTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYRRAAEAGVTGILAADLPPEEASDALRAAEKYDIDQIFIVAP
TTGSERLKRISEVSSGFHYLVSVMGVTGARSRVEDATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSA
IIDVISGNLGDRELMLQRIHEMAGTLKAAGGSG
>Mature_273_residues
MIVMSGAKSMSYAEMFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFSDPIADGTSVQGADLRALRAG
MTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYRRAAEAGVTGILAADLPPEEASDALRAAEKYDIDQIFIVAP
TTGSERLKRISEVSSGFHYLVSVMGVTGARSRVEDATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSA
IIDVISGNLGDRELMLQRIHEMAGTLKAAGGSG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=262, Percent_Identity=43.1297709923664, Blast_Score=211, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6321412, Length=228, Percent_Identity=39.4736842105263, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29301; Mature: 29301

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVMSGAKSMSYAEMFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFS
CEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCC
DPIADGTSVQGADLRALRAGMTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYR
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHH
RAAEAGVTGILAADLPPEEASDALRAAEKYDIDQIFIVAPTTGSERLKRISEVSSGFHYL
HHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
VSVMGVTGARSRVEDATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSA
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHH
IIDVISGNLGDRELMLQRIHEMAGTLKAAGGSG
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIVMSGAKSMSYAEMFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFS
CEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCC
DPIADGTSVQGADLRALRAGMTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYR
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHH
RAAEAGVTGILAADLPPEEASDALRAAEKYDIDQIFIVAPTTGSERLKRISEVSSGFHYL
HHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
VSVMGVTGARSRVEDATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSA
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHH
IIDVISGNLGDRELMLQRIHEMAGTLKAAGGSG
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]