Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 15679571

GI number: 15679571

Start: 1443295

End: 1444569

Strand: Direct

Name: Not Available

Synonym: MTH1576

Alternate gene names: 15679571

Gene position: 1443295-1444569 (Clockwise)

Preceding gene: 15679569

Following gene: 15679572

Centisome position: 82.41

GC content: 52.63

Gene sequence:

>1275_bases
GTGACTCAGATGGAAGAAGCAAGAAAGGGCAATATAACCCCTGAGATGGAGGAGGTTGCCCGGAAAGAAAACCTGGACAT
TCAGATGCTTATTAGGGGTATTGCAAACGGTCGAATTGTGATACCCTCCAATATTAATCGGGAATCATCACCCTGCGGCA
TAGGGGAAAATCTTTCAACAAAGATCAATGCAAATATCGGTTCATCCTCAAAAATGGAGGACATTGAACTGGAGGTTGAT
AAGGCACTTGCAGCTGTCGAATACGGTGCAGATGCTGTCATGGACCTCAGCACAGGCCCCATGCTGAGGGATGTCAGGAA
AGCGGTCCTGGAGGCTGTTGACGTTCCTGTGGGCACGGTACCCATCTACGAGGCCGGTGTCGAGGCCTTCATGTCAGATG
GAGCCGTGGTGGACATGGATGAGGATGACATGTTCAGGGCCATAGAGAACCAGGCCCGGGATGGGGTTGACTTCATGACC
GTGCACTCAGGAATCACCCTGGAAACCGTTGAAAGGGCTCAGAGATCAGGGAGGATAATGGGAATAGTTAGCCGGGGAGG
AGCATTCCTTGCAGCCTGGATAATGCAGAATCAGGAGGAGAATCCTCTCTACAGCAACTATGAGTACCTCCTTGAAGTGG
CCTACGAGTATGATGTCACACTCAGCCTCGGGGATGGCCTCAGGCCGGGGTGCCTGGCAGACGCTTCTGATATACCCCAG
ATCAGTGAGCTCCTCACACTTGCAGAACTCGTTGAGAGGGCACGTGATGCAGATGTTCAGTGCATGGTTGAGGGGCCCGG
TCACATGCCCCTTGACCAGATAGCCGCCAACATGAAGATACAGAAGGAGGTCTGCGACGGCGCACCCTTCTATGTCCTTG
GACCCATAGTCACAGACATGGCACCGGGTTATGACCATATAAGCGCAGCCATAGGCGGGGCCGTCGCTGCAATGAACGGG
GCGGACTTCCTCTGTTACGTCACACCGGCAGAGCACCTGGCGATACCGGGGGTTCAGGATGTTATTGAGGGTGTCATTGC
ATCAAGGATAGCTGCCCAGGCTGCAGACGCCGCCAGAAAATTACCGGGCGCATGGGATTCTGAACTTGAGATGGCTGATG
CAAGAAGGTCCTTTGACTGGGATAAACAGTTCAAACTGGCCTTTGACTCAAAAAAACCATATGAATACAGGATGCAGTGC
CCAATTGAGGATTCAGAGATGTGCTCAATGTGTGGCGAATACTGCGCCCTCAGGATACTGCGTGAAGACAGATGA

Upstream 100 bases:

>100_bases
GAAACAATAACATCACGGAGCATTATATCCCTTGCACGGAGCTTCCTGATCATGAAATTATTTTTATGGATATTCATATA
TTAAGATTGAGGGATCCAAA

Downstream 100 bases:

>100_bases
ATCACAGCCTCGATCCTTAACATGCTGGTAAGGGTTAATAGAGATCAGAACCATAATTAAACTGGTGGTCCTATGGGATT
CGAGCATGTTGAAGGTACTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 2; HMP-P synthase 2; HMP-phosphate synthase 2; HMPP synthase 2; Thiamine biosynthesis protein thiC 2

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTQMEEARKGNITPEMEEVARKENLDIQMLIRGIANGRIVIPSNINRESSPCGIGENLSTKINANIGSSSKMEDIELEVD
KALAAVEYGADAVMDLSTGPMLRDVRKAVLEAVDVPVGTVPIYEAGVEAFMSDGAVVDMDEDDMFRAIENQARDGVDFMT
VHSGITLETVERAQRSGRIMGIVSRGGAFLAAWIMQNQEENPLYSNYEYLLEVAYEYDVTLSLGDGLRPGCLADASDIPQ
ISELLTLAELVERARDADVQCMVEGPGHMPLDQIAANMKIQKEVCDGAPFYVLGPIVTDMAPGYDHISAAIGGAVAAMNG
ADFLCYVTPAEHLAIPGVQDVIEGVIASRIAAQAADAARKLPGAWDSELEMADARRSFDWDKQFKLAFDSKKPYEYRMQC
PIEDSEMCSMCGEYCALRILREDR

Sequences:

>Translated_424_residues
MTQMEEARKGNITPEMEEVARKENLDIQMLIRGIANGRIVIPSNINRESSPCGIGENLSTKINANIGSSSKMEDIELEVD
KALAAVEYGADAVMDLSTGPMLRDVRKAVLEAVDVPVGTVPIYEAGVEAFMSDGAVVDMDEDDMFRAIENQARDGVDFMT
VHSGITLETVERAQRSGRIMGIVSRGGAFLAAWIMQNQEENPLYSNYEYLLEVAYEYDVTLSLGDGLRPGCLADASDIPQ
ISELLTLAELVERARDADVQCMVEGPGHMPLDQIAANMKIQKEVCDGAPFYVLGPIVTDMAPGYDHISAAIGGAVAAMNG
ADFLCYVTPAEHLAIPGVQDVIEGVIASRIAAQAADAARKLPGAWDSELEMADARRSFDWDKQFKLAFDSKKPYEYRMQC
PIEDSEMCSMCGEYCALRILREDR
>Mature_423_residues
TQMEEARKGNITPEMEEVARKENLDIQMLIRGIANGRIVIPSNINRESSPCGIGENLSTKINANIGSSSKMEDIELEVDK
ALAAVEYGADAVMDLSTGPMLRDVRKAVLEAVDVPVGTVPIYEAGVEAFMSDGAVVDMDEDDMFRAIENQARDGVDFMTV
HSGITLETVERAQRSGRIMGIVSRGGAFLAAWIMQNQEENPLYSNYEYLLEVAYEYDVTLSLGDGLRPGCLADASDIPQI
SELLTLAELVERARDADVQCMVEGPGHMPLDQIAANMKIQKEVCDGAPFYVLGPIVTDMAPGYDHISAAIGGAVAAMNGA
DFLCYVTPAEHLAIPGVQDVIEGVIASRIAAQAADAARKLPGAWDSELEMADARRSFDWDKQFKLAFDSKKPYEYRMQCP
IEDSEMCSMCGEYCALRILREDR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=457, Percent_Identity=42.0131291028446, Blast_Score=355, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC2_METTH (O27617)

Other databases:

- EMBL:   AE000666
- PIR:   C69077
- RefSeq:   NP_276688.1
- ProteinModelPortal:   O27617
- STRING:   O27617
- GeneID:   1471845
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1576
- NMPDR:   fig|187420.1.peg.1543
- eggNOG:   arNOG04546
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- PhylomeDB:   O27617
- ProtClustDB:   PRK13352
- BioCyc:   MTHE187420:MTH1576-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 46315; Mature: 46184

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMEEARKGNITPEMEEVARKENLDIQMLIRGIANGRIVIPSNINRESSPCGIGENLST
CCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCE
KINANIGSSSKMEDIELEVDKALAAVEYGADAVMDLSTGPMLRDVRKAVLEAVDVPVGTV
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCC
PIYEAGVEAFMSDGAVVDMDEDDMFRAIENQARDGVDFMTVHSGITLETVERAQRSGRIM
HHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEE
GIVSRGGAFLAAWIMQNQEENPLYSNYEYLLEVAYEYDVTLSLGDGLRPGCLADASDIPQ
EEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHH
ISELLTLAELVERARDADVQCMVEGPGHMPLDQIAANMKIQKEVCDGAPFYVLGPIVTDM
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHCCCCEEEECHHHHHC
APGYDHISAAIGGAVAAMNGADFLCYVTPAEHLAIPGVQDVIEGVIASRIAAQAADAARK
CCCHHHHHHHHHHHHEEECCCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LPGAWDSELEMADARRSFDWDKQFKLAFDSKKPYEYRMQCPIEDSEMCSMCGEYCALRIL
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
REDR
HCCC
>Mature Secondary Structure 
TQMEEARKGNITPEMEEVARKENLDIQMLIRGIANGRIVIPSNINRESSPCGIGENLST
CCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCE
KINANIGSSSKMEDIELEVDKALAAVEYGADAVMDLSTGPMLRDVRKAVLEAVDVPVGTV
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCC
PIYEAGVEAFMSDGAVVDMDEDDMFRAIENQARDGVDFMTVHSGITLETVERAQRSGRIM
HHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEE
GIVSRGGAFLAAWIMQNQEENPLYSNYEYLLEVAYEYDVTLSLGDGLRPGCLADASDIPQ
EEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHH
ISELLTLAELVERARDADVQCMVEGPGHMPLDQIAANMKIQKEVCDGAPFYVLGPIVTDM
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHCCCCEEEECHHHHHC
APGYDHISAAIGGAVAAMNGADFLCYVTPAEHLAIPGVQDVIEGVIASRIAAQAADAARK
CCCHHHHHHHHHHHHEEECCCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LPGAWDSELEMADARRSFDWDKQFKLAFDSKKPYEYRMQCPIEDSEMCSMCGEYCALRIL
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
REDR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463