Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 15679525

GI number: 15679525

Start: 1389398

End: 1390645

Strand: Direct

Name: Not Available

Synonym: MTH1529

Alternate gene names: 15679525

Gene position: 1389398-1390645 (Clockwise)

Preceding gene: 15679524

Following gene: 15679526

Centisome position: 79.33

GC content: 52.88

Gene sequence:

>1248_bases
ATGCTGAATGCAGAAACCTATGTCACATCAACAGAGGGGACCGGTGGAAGAATAAGGGTGCATAACAGGGACTTTGAGGT
GGAGGAGATCCCCCTCACAGAGCCCAGTGGCAGCGGCCCAAACACATGGATATGGATAGAGAAGGAGGGCAGGACAACCC
TCGACGTCCTCCTCGACATTGCAAGGGAACTCCACCTTGACAGGAGAAGGATGGGATTCGCTGGCATGAAGGATAAGAGG
GCCGTCACCAGACAGTGGATATGTGTGAGCAACACAGCACCCTCAGAGGTTAAGGCCATAGAGGAAAGGATAAGAAACGT
TAAATTCCTCAGGGTCACAGCAAATGAGAAGAAACTCCGGATGGGCCAGCTGTTGGGCAACCGTTTCAGGATCCTGATAA
GGGACACCGAAATCGATGAACCCCTGGAAACAGCCAAGGCCACCCTCCAGGAACTTGAGGATAAGGGTGTCCCGAATTAC
TATGGCTGGCAGCGCTTTGGGAGTCCAAGGGCAAACACGCACCTTGTTGGACGGGCACTTGTCCATGGCGATGTAAAGGG
CGCGGTTGACAGCTACATAGGTAACCCCCTTGAGGGGGAATCAGAGATTGTTTCAGAGGCCAGGAGGGCCTATGACAGGG
GCGACCTTGAGGAGGCATATGAGCTCATGCCATCATCAATGAGGTATGAGAGGATGATGCTGAGACCGATGCTCAGGGAT
CTACGTAGGGGTGAGTTCTCAGATGAATCATACGTGAGGGCCATCCATGCACTTCCGAAGCCCCTGAAGAGGATGTTCGT
CCACGCCTACCAGTCATACCTCTTCAACCGGGCAGTAAGTGAGAGGGCGGCCCTCGGCATAAACACCCACATCCCCGGCG
ATATAGTCATTGATAATGACCAGCACATAATACATGACGCTGATCCAGATGAACTGGAGGAGCTGATCCTAAACTTCGAG
GCCCATCCGACAGCACCCTTATATGGCAGCAAGGTCCCCCTTGCCAGTGGAAGGCCCGGTGAGATTGAGAGGAGGATACT
TGAGGATGAGGGAGTGTCCCTTGAGGACTTCAACTCAATAAAGGTCCCCAAACTTGGAAGCCATGGCATGAGGAGGGCGA
TACGCTTCAGGATATGGGACGTGGATGCCCATGAGAGCCAGGAGGGCCTTACAGTTGAATTCTCAATACCCAGGGGGTGC
TATGCAACCTCTGTTTTAAGGGAGATCATGAAAAAGGATGTTGTCTAG

Upstream 100 bases:

>100_bases
GGAACTGAGCCTTCCTTCAGTGGATAGCGAATTATATAATTAAAATGGTGCTGAATGCAGAAACATTATCGACTGAGTAC
ATAAATTCTGGAGTAAAACG

Downstream 100 bases:

>100_bases
GTCTTCAGACATGCAGGTGGGTCCATGGATTTTACTGAATATCCGTGACCACTTACTGAATGATTAAAGGTATGGAGATG
ATCCGGTGGAAGTTGACTGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLNAETYVTSTEGTGGRIRVHNRDFEVEEIPLTEPSGSGPNTWIWIEKEGRTTLDVLLDIARELHLDRRRMGFAGMKDKR
AVTRQWICVSNTAPSEVKAIEERIRNVKFLRVTANEKKLRMGQLLGNRFRILIRDTEIDEPLETAKATLQELEDKGVPNY
YGWQRFGSPRANTHLVGRALVHGDVKGAVDSYIGNPLEGESEIVSEARRAYDRGDLEEAYELMPSSMRYERMMLRPMLRD
LRRGEFSDESYVRAIHALPKPLKRMFVHAYQSYLFNRAVSERAALGINTHIPGDIVIDNDQHIIHDADPDELEELILNFE
AHPTAPLYGSKVPLASGRPGEIERRILEDEGVSLEDFNSIKVPKLGSHGMRRAIRFRIWDVDAHESQEGLTVEFSIPRGC
YATSVLREIMKKDVV

Sequences:

>Translated_415_residues
MLNAETYVTSTEGTGGRIRVHNRDFEVEEIPLTEPSGSGPNTWIWIEKEGRTTLDVLLDIARELHLDRRRMGFAGMKDKR
AVTRQWICVSNTAPSEVKAIEERIRNVKFLRVTANEKKLRMGQLLGNRFRILIRDTEIDEPLETAKATLQELEDKGVPNY
YGWQRFGSPRANTHLVGRALVHGDVKGAVDSYIGNPLEGESEIVSEARRAYDRGDLEEAYELMPSSMRYERMMLRPMLRD
LRRGEFSDESYVRAIHALPKPLKRMFVHAYQSYLFNRAVSERAALGINTHIPGDIVIDNDQHIIHDADPDELEELILNFE
AHPTAPLYGSKVPLASGRPGEIERRILEDEGVSLEDFNSIKVPKLGSHGMRRAIRFRIWDVDAHESQEGLTVEFSIPRGC
YATSVLREIMKKDVV
>Mature_415_residues
MLNAETYVTSTEGTGGRIRVHNRDFEVEEIPLTEPSGSGPNTWIWIEKEGRTTLDVLLDIARELHLDRRRMGFAGMKDKR
AVTRQWICVSNTAPSEVKAIEERIRNVKFLRVTANEKKLRMGQLLGNRFRILIRDTEIDEPLETAKATLQELEDKGVPNY
YGWQRFGSPRANTHLVGRALVHGDVKGAVDSYIGNPLEGESEIVSEARRAYDRGDLEEAYELMPSSMRYERMMLRPMLRD
LRRGEFSDESYVRAIHALPKPLKRMFVHAYQSYLFNRAVSERAALGINTHIPGDIVIDNDQHIIHDADPDELEELILNFE
AHPTAPLYGSKVPLASGRPGEIERRILEDEGVSLEDFNSIKVPKLGSHGMRRAIRFRIWDVDAHESQEGLTVEFSIPRGC
YATSVLREIMKKDVV

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI148762985, Length=421, Percent_Identity=28.0285035629454, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI148762961, Length=421, Percent_Identity=28.0285035629454, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI148762948, Length=421, Percent_Identity=28.0285035629454, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI50727002, Length=413, Percent_Identity=24.6973365617433, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1789102, Length=167, Percent_Identity=32.9341317365269, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557232, Length=398, Percent_Identity=24.8743718592965, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324817, Length=290, Percent_Identity=29.3103448275862, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21355799, Length=259, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_METTH (O27572)

Other databases:

- EMBL:   AE000666
- PIR:   H69070
- RefSeq:   NP_276642.1
- ProteinModelPortal:   O27572
- SMR:   O27572
- STRING:   O27572
- GeneID:   1471798
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1529
- NMPDR:   fig|187420.1.peg.1497
- eggNOG:   arNOG05422
- HOGENOM:   HBG541221
- OMA:   WAGTKDK
- ProtClustDB:   CLSK798190
- BioCyc:   MTHE187420:MTH1529-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 47347; Mature: 47347

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAETYVTSTEGTGGRIRVHNRDFEVEEIPLTEPSGSGPNTWIWIEKEGRTTLDVLLDI
CCCCCEEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
ARELHLDRRRMGFAGMKDKRAVTRQWICVSNTAPSEVKAIEERIRNVKFLRVTANEKKLR
HHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCHHHH
MGQLLGNRFRILIRDTEIDEPLETAKATLQELEDKGVPNYYGWQRFGSPRANTHLVGRAL
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHH
VHGDVKGAVDSYIGNPLEGESEIVSEARRAYDRGDLEEAYELMPSSMRYERMMLRPMLRD
HHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
LRRGEFSDESYVRAIHALPKPLKRMFVHAYQSYLFNRAVSERAALGINTHIPGDIVIDND
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC
QHIIHDADPDELEELILNFEAHPTAPLYGSKVPLASGRPGEIERRILEDEGVSLEDFNSI
CEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCCCC
KVPKLGSHGMRRAIRFRIWDVDAHESQEGLTVEFSIPRGCYATSVLREIMKKDVV
CCCCCCCHHHHHEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNAETYVTSTEGTGGRIRVHNRDFEVEEIPLTEPSGSGPNTWIWIEKEGRTTLDVLLDI
CCCCCEEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
ARELHLDRRRMGFAGMKDKRAVTRQWICVSNTAPSEVKAIEERIRNVKFLRVTANEKKLR
HHHHHHHHHHHCCCCCCHHHHHHHEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCHHHH
MGQLLGNRFRILIRDTEIDEPLETAKATLQELEDKGVPNYYGWQRFGSPRANTHLVGRAL
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHH
VHGDVKGAVDSYIGNPLEGESEIVSEARRAYDRGDLEEAYELMPSSMRYERMMLRPMLRD
HHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
LRRGEFSDESYVRAIHALPKPLKRMFVHAYQSYLFNRAVSERAALGINTHIPGDIVIDND
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC
QHIIHDADPDELEELILNFEAHPTAPLYGSKVPLASGRPGEIERRILEDEGVSLEDFNSI
CEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCCCC
KVPKLGSHGMRRAIRFRIWDVDAHESQEGLTVEFSIPRGCYATSVLREIMKKDVV
CCCCCCCHHHHHEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463