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The map label for this gene is gatB

Identifier: 15679284

GI number: 15679284

Start: 1162294

End: 1163643

Strand: Direct

Name: gatB

Synonym: MTH1280

Alternate gene names: 15679284

Gene position: 1162294-1163643 (Clockwise)

Preceding gene: 15679283

Following gene: 15679285

Centisome position: 66.36

GC content: 52.07

Gene sequence:

>1350_bases
ATGAAATGTGGTCTTGAAATTCATGTTCAGCTTGACACCAGATCAAAACTCTTCTGCAGGTGCCCCACAGATTACCAGGA
AGCCCCACCAAACACCAACATATGCCCGGTCTGCCTCAACCAGCCCGGGGCCAAGCCATACCCCCCAAACAGGAGCGCCC
TTGAGGGGGCACTTAAAATTGCACTCATGCTCGATTGCAGGATAAACCCTGAGATCACCTACTTCATGCGTAAGCACTAC
AACTACCCGGACCTCCCATCCGGGTATCAGAGGACATCCATACCCGTGGGACTTGAGGGTGAATTGAACGGTGTGAGGAT
CAGGGAGGTCCACATCGAGGAGGACCCGGGCCAGTACAAGCCCGACCAGGGAACCGTGGACTTCAACAGGTCAGGAATCC
CCCTCATTGAAATAGTCACAGAACCCGACATGACCTCCCCTGAGGAGGCCAGGAACTTCCTCAGGGAACTCATAAGGGTC
CTCGAGTACAGTGGCTGTGCACGTGGGGAGGGTACAATGAGGGCCGACGTCAACATATCCCTTGAGGGTGGTAAAAGGGT
TGAAATAAAGAACATAAACTCAATTAAGGGCGCCTACAAGGCCCTGAAATTTGAGATGATCAGACAGAAGAACCTCCTGA
AGAGGGGTGTTGAGGTCAAGCAGGAAACAAGGGCCTTTCTTGAGTCGCAGATGATAACTGTTTCAATGAGGCTCAAGGAG
GAGGCAGAGGACTACAGATACATACCTGACCCGGACCTTCCGCCAATGAAGTTCACGGATGAAGTGGTGGAGGGTCTGCG
GGAGAGGATACCGGAAGCACCCCACCTGAAGGTGAAGCGTTTCATGGAGGAGTACGGCCTGAGGGAGGAGGATGCAAGGG
TCCTCACATCAGAGCTGGAGCTTGCAGACGCGTTCGAGGAGGTTGCTGGCTCAATAGACCCTGAATTTGCTGCCCTGTGG
ATGAGGGATGAACTGAAAAGGGTCCTTTACTACAACAAGATAAGCTTTGCAGAAAGCATGATAACACCAGGGGATATCAT
CGAGCTTCTCGCCATGATCAGGAAGAAGGAGATAACAAGCAAGGCAGCCAAGAAGATCATAGAGGAGATGCCACTGAATA
AAAAGGGTCCCCGGGAGATAGCGACCGAAATGGGACTCATAGGCATCATAGATGAATCGGAAGTCATAAGGGCCGTTGAA
CAGGCCATAAGAGAGAATCCAGGGGCTGTTGAGGATTACCATGCCGGTAAGGAGGCAGCCATAAATTTCCTGGTTGGTCA
GGTCATGAGGATGACCCGTGGTAAGGCTGAACCCGAACGCACCGTTGAACTGATAAAGGAACGGATCTGA

Upstream 100 bases:

>100_bases
TGAGGGGTGTCTGAAAAATCTTAAATTGCTTTAAGTAAATGATGATAATAATATACTCTGAAGTTCACCGGATCATAAAA
AGGGAGGAATAACCATTAAG

Downstream 100 bases:

>100_bases
GGTGTATGGCCAAGAACGTCCTGGATCTTCTGATGTGGCATCCGAAGTGGGATATTGAAAGATGCAGGATAAGCTACCTC
CACAGGGGTGCCTGCAGGAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKCGLEIHVQLDTRSKLFCRCPTDYQEAPPNTNICPVCLNQPGAKPYPPNRSALEGALKIALMLDCRINPEITYFMRKHY
NYPDLPSGYQRTSIPVGLEGELNGVRIREVHIEEDPGQYKPDQGTVDFNRSGIPLIEIVTEPDMTSPEEARNFLRELIRV
LEYSGCARGEGTMRADVNISLEGGKRVEIKNINSIKGAYKALKFEMIRQKNLLKRGVEVKQETRAFLESQMITVSMRLKE
EAEDYRYIPDPDLPPMKFTDEVVEGLRERIPEAPHLKVKRFMEEYGLREEDARVLTSELELADAFEEVAGSIDPEFAALW
MRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITSKAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVE
QAIRENPGAVEDYHAGKEAAINFLVGQVMRMTRGKAEPERTVELIKERI

Sequences:

>Translated_449_residues
MKCGLEIHVQLDTRSKLFCRCPTDYQEAPPNTNICPVCLNQPGAKPYPPNRSALEGALKIALMLDCRINPEITYFMRKHY
NYPDLPSGYQRTSIPVGLEGELNGVRIREVHIEEDPGQYKPDQGTVDFNRSGIPLIEIVTEPDMTSPEEARNFLRELIRV
LEYSGCARGEGTMRADVNISLEGGKRVEIKNINSIKGAYKALKFEMIRQKNLLKRGVEVKQETRAFLESQMITVSMRLKE
EAEDYRYIPDPDLPPMKFTDEVVEGLRERIPEAPHLKVKRFMEEYGLREEDARVLTSELELADAFEEVAGSIDPEFAALW
MRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITSKAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVE
QAIRENPGAVEDYHAGKEAAINFLVGQVMRMTRGKAEPERTVELIKERI
>Mature_449_residues
MKCGLEIHVQLDTRSKLFCRCPTDYQEAPPNTNICPVCLNQPGAKPYPPNRSALEGALKIALMLDCRINPEITYFMRKHY
NYPDLPSGYQRTSIPVGLEGELNGVRIREVHIEEDPGQYKPDQGTVDFNRSGIPLIEIVTEPDMTSPEEARNFLRELIRV
LEYSGCARGEGTMRADVNISLEGGKRVEIKNINSIKGAYKALKFEMIRQKNLLKRGVEVKQETRAFLESQMITVSMRLKE
EAEDYRYIPDPDLPPMKFTDEVVEGLRERIPEAPHLKVKRFMEEYGLREEDARVLTSELELADAFEEVAGSIDPEFAALW
MRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITSKAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVE
QAIRENPGAVEDYHAGKEAAINFLVGQVMRMTRGKAEPERTVELIKERI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=491, Percent_Identity=32.7902240325866, Blast_Score=243, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI115534094, Length=368, Percent_Identity=35.054347826087, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319391, Length=499, Percent_Identity=28.8577154308617, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI45550808, Length=484, Percent_Identity=31.8181818181818, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_METTH (O27341)

Other databases:

- EMBL:   AE000666
- PIR:   D69037
- RefSeq:   NP_276401.1
- ProteinModelPortal:   O27341
- SMR:   O27341
- STRING:   O27341
- GeneID:   1471688
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1280
- NMPDR:   fig|187420.1.peg.1256
- eggNOG:   arNOG04834
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   MTHE187420:MTH1280-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 51133; Mature: 51133

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCGLEIHVQLDTRSKLFCRCPTDYQEAPPNTNICPVCLNQPGAKPYPPNRSALEGALKI
CCCCEEEEEEECCCCCEEEECCCCHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHEE
ALMLDCRINPEITYFMRKHYNYPDLPSGYQRTSIPVGLEGELNGVRIREVHIEEDPGQYK
EEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
PDQGTVDFNRSGIPLIEIVTEPDMTSPEEARNFLRELIRVLEYSGCARGEGTMRADVNIS
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
LEGGKRVEIKNINSIKGAYKALKFEMIRQKNLLKRGVEVKQETRAFLESQMITVSMRLKE
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEHHHHH
EAEDYRYIPDPDLPPMKFTDEVVEGLRERIPEAPHLKVKRFMEEYGLREEDARVLTSELE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LADAFEEVAGSIDPEFAALWMRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVEQAIRENPGAVEDYHAGKEAA
HHHHHHHHHCCCCCCCHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCHHHCCCHHHH
INFLVGQVMRMTRGKAEPERTVELIKERI
HHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKCGLEIHVQLDTRSKLFCRCPTDYQEAPPNTNICPVCLNQPGAKPYPPNRSALEGALKI
CCCCEEEEEEECCCCCEEEECCCCHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHEE
ALMLDCRINPEITYFMRKHYNYPDLPSGYQRTSIPVGLEGELNGVRIREVHIEEDPGQYK
EEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
PDQGTVDFNRSGIPLIEIVTEPDMTSPEEARNFLRELIRVLEYSGCARGEGTMRADVNIS
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
LEGGKRVEIKNINSIKGAYKALKFEMIRQKNLLKRGVEVKQETRAFLESQMITVSMRLKE
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEHHHHH
EAEDYRYIPDPDLPPMKFTDEVVEGLRERIPEAPHLKVKRFMEEYGLREEDARVLTSELE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LADAFEEVAGSIDPEFAALWMRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVEQAIRENPGAVEDYHAGKEAA
HHHHHHHHHCCCCCCCHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCHHHCCCHHHH
INFLVGQVMRMTRGKAEPERTVELIKERI
HHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 10993083