Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 15679154

GI number: 15679154

Start: 1055708

End: 1056739

Strand: Direct

Name: Not Available

Synonym: MTH1143

Alternate gene names: 15679154

Gene position: 1055708-1056739 (Clockwise)

Preceding gene: 15679153

Following gene: 15679155

Centisome position: 60.28

GC content: 50.29

Gene sequence:

>1032_bases
TTGATAGATAAAATACTGAAAAAGGCGGCAGAAGGGTACCAGCTCCAGGATGGAGAGGTTATCCAGTTATTCAGAATAAG
AGAATCCGGGGATCTTGCCAGTCTCATGAAAACAGCCTTCAATATAATGCGTGAAAACCGCGGCTCTGTGAAGCTGACAT
CCACAGTGCACATAACAAACATGTGCCAGGTCAGGCCACGGTGCGGTTACTGTGGATTTGCTGCAGGCACATCAAAAAAT
GGTTACTATAACTCATTCTTCAAAACTGATGATGAGATCCTTGAAGCCGCCCTCATAATAGAGGAATCAGGAATACCCAG
GGTCAGCTGCTCAGGTGCCCATGGATTCAATGGTGAGCATGCCGTGAAGGCTGCCAGGATAGTTAAGGAGAACACATCAC
TTGAACTCCTCATAAACGTTGGATCTGACCTCAACAGGAGTGCCACAGAGAAACTTGCAGACTACGGGACAGACACTGTA
TGCTGCAACCTTGAAACCACCAACAGGGAACTTTTCAGCAGTGTTAAACCCGGTGAGAGAATCGAGGACAGGATCAGGGT
CTGTGAAATGGTTTGTGCGGCAGGTATTGAGCTCTCAAGTGGCCTGCTCATAGGTCTTGGTGAAAGCTACGCTGATCGCC
TGGAGCACCTTAAGTTTCTATCTAAATTTGAAACCCTTGGTGAGATACCCATAATGGGATTCAACCCCTACCCTGGCACT
CCAATGGAGGAACATCCGCCCTGCCCCCTGAGTGAGCAGATGAAGACCATAGCCATTACAAGGATCCTCTACCCTGACAT
AAGGATAACAGTTCCAACACCCACAATAGGGCCCGAGAATGTCAGGTTTTCCCTGATGGCCGGGGCCGATAACCTTGCAA
CTGTTATACCGGACGGCTACCCCCATGACGTTAAGGGTGTGGGGTCCCCCCGCTACGGGAACCTCAGGGACGTCCTGAAG
GTTGTGAAGGAAATGGGACTGAGGGCCCAGTTAAAACCTGCCCCCTCAGCCGAGGGAGTGGCTCTGCTCTGA

Upstream 100 bases:

>100_bases
AGGAAAATCCCAGAAAAATTTATTTATTTTTTTATCCAAGTAATTTTTACACTTCAATTTACTGACTTTTCAATCCCAAG
CAGCTGAAACTGGTGTTAAA

Downstream 100 bases:

>100_bases
TTGGGGGTGTTGAAATGCTTGAGGACCTCATAGGTAAAGCCTACCTTGAATCTGCAGAGGACAGACGCCGCGGGGACAGA
TCTGAAGAGGTTGAGGCCAT

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MIDKILKKAAEGYQLQDGEVIQLFRIRESGDLASLMKTAFNIMRENRGSVKLTSTVHITNMCQVRPRCGYCGFAAGTSKN
GYYNSFFKTDDEILEAALIIEESGIPRVSCSGAHGFNGEHAVKAARIVKENTSLELLINVGSDLNRSATEKLADYGTDTV
CCNLETTNRELFSSVKPGERIEDRIRVCEMVCAAGIELSSGLLIGLGESYADRLEHLKFLSKFETLGEIPIMGFNPYPGT
PMEEHPPCPLSEQMKTIAITRILYPDIRITVPTPTIGPENVRFSLMAGADNLATVIPDGYPHDVKGVGSPRYGNLRDVLK
VVKEMGLRAQLKPAPSAEGVALL

Sequences:

>Translated_343_residues
MIDKILKKAAEGYQLQDGEVIQLFRIRESGDLASLMKTAFNIMRENRGSVKLTSTVHITNMCQVRPRCGYCGFAAGTSKN
GYYNSFFKTDDEILEAALIIEESGIPRVSCSGAHGFNGEHAVKAARIVKENTSLELLINVGSDLNRSATEKLADYGTDTV
CCNLETTNRELFSSVKPGERIEDRIRVCEMVCAAGIELSSGLLIGLGESYADRLEHLKFLSKFETLGEIPIMGFNPYPGT
PMEEHPPCPLSEQMKTIAITRILYPDIRITVPTPTIGPENVRFSLMAGADNLATVIPDGYPHDVKGVGSPRYGNLRDVLK
VVKEMGLRAQLKPAPSAEGVALL
>Mature_343_residues
MIDKILKKAAEGYQLQDGEVIQLFRIRESGDLASLMKTAFNIMRENRGSVKLTSTVHITNMCQVRPRCGYCGFAAGTSKN
GYYNSFFKTDDEILEAALIIEESGIPRVSCSGAHGFNGEHAVKAARIVKENTSLELLINVGSDLNRSATEKLADYGTDTV
CCNLETTNRELFSSVKPGERIEDRIRVCEMVCAAGIELSSGLLIGLGESYADRLEHLKFLSKFETLGEIPIMGFNPYPGT
PMEEHPPCPLSEQMKTIAITRILYPDIRITVPTPTIGPENVRFSLMAGADNLATVIPDGYPHDVKGVGSPRYGNLRDVLK
VVKEMGLRAQLKPAPSAEGVALL

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786992, Length=236, Percent_Identity=26.6949152542373, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 37502; Mature: 37502

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKILKKAAEGYQLQDGEVIQLFRIRESGDLASLMKTAFNIMRENRGSVKLTSTVHITN
CHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEH
MCQVRPRCGYCGFAAGTSKNGYYNSFFKTDDEILEAALIIEESGIPRVSCSGAHGFNGEH
HEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCEEEECCCCCCCCHH
AVKAARIVKENTSLELLINVGSDLNRSATEKLADYGTDTVCCNLETTNRELFSSVKPGER
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCHH
IEDRIRVCEMVCAAGIELSSGLLIGLGESYADRLEHLKFLSKFETLGEIPIMGFNPYPGT
HHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
PMEEHPPCPLSEQMKTIAITRILYPDIRITVPTPTIGPENVRFSLMAGADNLATVIPDGY
CCCCCCCCCHHHHHHHHHHHHEECCCEEEEECCCCCCCCCEEEEEEECCCCCEEECCCCC
PHDVKGVGSPRYGNLRDVLKVVKEMGLRAQLKPAPSAEGVALL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEC
>Mature Secondary Structure
MIDKILKKAAEGYQLQDGEVIQLFRIRESGDLASLMKTAFNIMRENRGSVKLTSTVHITN
CHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEH
MCQVRPRCGYCGFAAGTSKNGYYNSFFKTDDEILEAALIIEESGIPRVSCSGAHGFNGEH
HEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCEEEECCCCCCCCHH
AVKAARIVKENTSLELLINVGSDLNRSATEKLADYGTDTVCCNLETTNRELFSSVKPGER
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCHH
IEDRIRVCEMVCAAGIELSSGLLIGLGESYADRLEHLKFLSKFETLGEIPIMGFNPYPGT
HHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
PMEEHPPCPLSEQMKTIAITRILYPDIRITVPTPTIGPENVRFSLMAGADNLATVIPDGY
CCCCCCCCCHHHHHHHHHHHHEECCCEEEEECCCCCCCCCEEEEEEECCCCCEEECCCCC
PHDVKGVGSPRYGNLRDVLKVVKEMGLRAQLKPAPSAEGVALL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]