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Identifier: 15679123

GI number: 15679123

Start: 1025384

End: 1026133

Strand: Direct

Name: Not Available

Synonym: MTH1112

Alternate gene names: 15679123

Gene position: 1025384-1026133 (Clockwise)

Preceding gene: 15679122

Following gene: 15679124

Centisome position: 58.55

GC content: 57.6

Gene sequence:

>750_bases
ATGAACGTCACCGGCAAACTCAGGGGACTCATATCCTTCTCAACGGTTCTGCCCCTGAAATCAGAGGCCAGCATTGAGGA
TATAGCTTCACTCACACTCCACTGGCCCCTTGTGGGCGCCCTCATAGGGGTTGCAGTGGGTTCAGCCGGGGTGGCTGCAT
CCCTAGTGTTCCCGGGGGCGGTTGTTGCCTGTGTTGCCTATGGATTCGCCATCTGGTTCACAGGTTTCCACCACCTCGAT
GGCCTCATAGATATGGGTGACGCCCTCATGTCCCATGGAAGCTTTGAGAGGAAGATAGAGATAATGAGGGATCCCAGGAT
AGGCACAGGTGGCCTTGGACTTCTGCTCATCGTATCATCAACCACCATAGCAGCCATCTATTCCCTTCCACAGGGTGTCC
TGTTCCAGGGACTCCTCATAGGGGAGGTCTCGGCCAAGGTGTGCCTCACGGGCTGCGCCCGGGTATCCAGGCCCCTCGAT
GGTGGGACCGGGAGGCACTTCATAATGGCGAGTAGAAGCCCCTTCACCGGCATGGTCTGGATCTTCTGGGCCGTTGCAGC
ATACCTCCTGGCGGGGGTTCCCGGTGCTGTTAGTGTTGCTGTTGCTGTCCTCTCAGGAGTCTTCATTGGTCTTGTTGCCA
GGAGGAACTTCTACTGGAGCACGGGTGACGTGCTGGGGGCCTCGAATGAGGTGGGGAGGATGATGTCACTTCTGGGCCTC
CTTGCGGCCATCAGATTAACGGGGCTGTAG

Upstream 100 bases:

>100_bases
ATGGGGGTAGACCTGTGAGGACAGCAAGATCCGCCAGAACCCTGTTTACACCACTCCCCTCACCACAGACAGAAAGATCC
GCCTGCGAGGGGGGTGCCTG

Downstream 100 bases:

>100_bases
GTAAAGGGGGTGCCCATCCAGCATGTGGAGAGTTTAATGGTATTTAATGATTCATTGGAGGTCAGTATAATGGATGTGAA
GGTTTTGAGGCTTGGTCACA

Product: cobalamin (5'-phosphate) synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNVTGKLRGLISFSTVLPLKSEASIEDIASLTLHWPLVGALIGVAVGSAGVAASLVFPGAVVACVAYGFAIWFTGFHHLD
GLIDMGDALMSHGSFERKIEIMRDPRIGTGGLGLLLIVSSTTIAAIYSLPQGVLFQGLLIGEVSAKVCLTGCARVSRPLD
GGTGRHFIMASRSPFTGMVWIFWAVAAYLLAGVPGAVSVAVAVLSGVFIGLVARRNFYWSTGDVLGASNEVGRMMSLLGL
LAAIRLTGL

Sequences:

>Translated_249_residues
MNVTGKLRGLISFSTVLPLKSEASIEDIASLTLHWPLVGALIGVAVGSAGVAASLVFPGAVVACVAYGFAIWFTGFHHLD
GLIDMGDALMSHGSFERKIEIMRDPRIGTGGLGLLLIVSSTTIAAIYSLPQGVLFQGLLIGEVSAKVCLTGCARVSRPLD
GGTGRHFIMASRSPFTGMVWIFWAVAAYLLAGVPGAVSVAVAVLSGVFIGLVARRNFYWSTGDVLGASNEVGRMMSLLGL
LAAIRLTGL
>Mature_249_residues
MNVTGKLRGLISFSTVLPLKSEASIEDIASLTLHWPLVGALIGVAVGSAGVAASLVFPGAVVACVAYGFAIWFTGFHHLD
GLIDMGDALMSHGSFERKIEIMRDPRIGTGGLGLLLIVSSTTIAAIYSLPQGVLFQGLLIGEVSAKVCLTGCARVSRPLD
GGTGRHFIMASRSPFTGMVWIFWAVAAYLLAGVPGAVSVAVAVLSGVFIGLVARRNFYWSTGDVLGASNEVGRMMSLLGL
LAAIRLTGL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_METTH (O27184)

Other databases:

- EMBL:   AE000666
- PIR:   A69015
- RefSeq:   NP_276240.1
- STRING:   O27184
- GeneID:   1471520
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1112
- NMPDR:   fig|187420.1.peg.1095
- eggNOG:   arNOG07851
- HOGENOM:   HBG551914
- OMA:   KSEASIE
- ProtClustDB:   CLSK2750504
- BioCyc:   MetaCyc:MONOMER-14886
- BioCyc:   MTHE187420:MTH1112-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 25906; Mature: 25906

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xcc4ebac)-; HASH(0xcb0c3d0)-; HASH(0x43973720)-; HASH(0xc4bdb7c)-; HASH(0xc441484)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTGKLRGLISFSTVLPLKSEASIEDIASLTLHWPLVGALIGVAVGSAGVAASLVFPGA
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVACVAYGFAIWFTGFHHLDGLIDMGDALMSHGSFERKIEIMRDPRIGTGGLGLLLIVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEHH
TTIAAIYSLPQGVLFQGLLIGEVSAKVCLTGCARVSRPLDGGTGRHFIMASRSPFTGMVW
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHH
IFWAVAAYLLAGVPGAVSVAVAVLSGVFIGLVARRNFYWSTGDVLGASNEVGRMMSLLGL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHH
LAAIRLTGL
HHHHHHCCH
>Mature Secondary Structure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HHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9371463