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Identifier: 15678650

GI number: 15678650

Start: 556730

End: 557590

Strand: Direct

Name: Not Available

Synonym: MTH622

Alternate gene names: 15678650

Gene position: 556730-557590 (Clockwise)

Preceding gene: 15678649

Following gene: 15678655

Centisome position: 31.79

GC content: 54.82

Gene sequence:

>861_bases
ATGGAACCATATGGTAAGGGGGACGGCCGTGTCAGGTGGGTGACGCCTGAATGGCTGATGGAGAATATGGAGGATGTTGC
CATAATAGACTGTCAGCCAAACATCCACGACTACATACTTGAACACATACCCGGAGCAGTTTACCTCAACGAGGGCCTCT
TCAGGGAGCCCCGTGGGAAGGCACCGGCGATGTACATCCCTGAGGGAGCAGTCGAGCTGATATTCCAGCAGGCAGGGATC
GAAAACAGGCCCACCGTTGTATACACAGGTACCGGCGGAGTCAAGGGCTGGGGTGATGGTCTCGAGCAGACCATGGTGGC
CTACAGCCTTGCAAGGTTTGGCCATGAAAACATTCTGGTACTCAATGGCGGACTCGCAGAGTGGAAGAGGGCCGGTGGAG
AATTAACAAAGGTATTCCCTGAGGTTGAGGAATCCGGTTTCTCTGCTGTGACAAAGGAGGACTTCTACATAGAGTACCCT
GAATTCAAAAGGATAAAGGACGATGAAGACGTTCTGCTGCTCGATGCAAGACCGGCAGAGGTCTATGAGGGGCAGGGCCC
CTGGATAAAACCCGGGCACATACCGGGCGCAGTTAACCTCCCCTGGGCTGACCTCATGGACCCAGAAAACAGGACCCTGC
TGAAGCCAGAGGATGAGATACTTGAACTCGTAAATTCAGTGGGTGCAACACCCGACAGGAAGATAATCTGCAGCTGCGGC
ACCGGCAGGGAGGCGACGAACGAGTTCCTCCTCTTCAGGTGGTACCTTGGGTACCCTGATGTCAGGATATATGAGGGATC
ATTCACCGAGTGGACCCAGATAGAGGACAACCCCACAGTCACGGGTCCGGACCCACGCTGA

Upstream 100 bases:

>100_bases
TCAACCGATCTCTTTAATTGTTTCTTATTTTCATGCTTCCATCGAAAAATGTATATCTATCGATGACATACTTATTACAT
AATATGTTAGGAGTGATTCC

Downstream 100 bases:

>100_bases
TGAACCCTCAAATTATTTTTTTATCCATGAAAAATTCAGTGCCCCTGACCACCGAAGTCTGTTTTAAGTTCTGATCCCGC
AAATAACATCACAGCAAGGG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MEPYGKGDGRVRWVTPEWLMENMEDVAIIDCQPNIHDYILEHIPGAVYLNEGLFREPRGKAPAMYIPEGAVELIFQQAGI
ENRPTVVYTGTGGVKGWGDGLEQTMVAYSLARFGHENILVLNGGLAEWKRAGGELTKVFPEVEESGFSAVTKEDFYIEYP
EFKRIKDDEDVLLLDARPAEVYEGQGPWIKPGHIPGAVNLPWADLMDPENRTLLKPEDEILELVNSVGATPDRKIICSCG
TGREATNEFLLFRWYLGYPDVRIYEGSFTEWTQIEDNPTVTGPDPR

Sequences:

>Translated_286_residues
MEPYGKGDGRVRWVTPEWLMENMEDVAIIDCQPNIHDYILEHIPGAVYLNEGLFREPRGKAPAMYIPEGAVELIFQQAGI
ENRPTVVYTGTGGVKGWGDGLEQTMVAYSLARFGHENILVLNGGLAEWKRAGGELTKVFPEVEESGFSAVTKEDFYIEYP
EFKRIKDDEDVLLLDARPAEVYEGQGPWIKPGHIPGAVNLPWADLMDPENRTLLKPEDEILELVNSVGATPDRKIICSCG
TGREATNEFLLFRWYLGYPDVRIYEGSFTEWTQIEDNPTVTGPDPR
>Mature_286_residues
MEPYGKGDGRVRWVTPEWLMENMEDVAIIDCQPNIHDYILEHIPGAVYLNEGLFREPRGKAPAMYIPEGAVELIFQQAGI
ENRPTVVYTGTGGVKGWGDGLEQTMVAYSLARFGHENILVLNGGLAEWKRAGGELTKVFPEVEESGFSAVTKEDFYIEYP
EFKRIKDDEDVLLLDARPAEVYEGQGPWIKPGHIPGAVNLPWADLMDPENRTLLKPEDEILELVNSVGATPDRKIICSCG
TGREATNEFLLFRWYLGYPDVRIYEGSFTEWTQIEDNPTVTGPDPR

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains

Homologues:

Organism=Homo sapiens, GI194473668, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI61835204, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI194473681, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI17402865, Length=241, Percent_Identity=25.7261410788382, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87082121, Length=283, Percent_Identity=27.9151943462898, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081967, Length=261, Percent_Identity=22.6053639846743, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71997283, Length=304, Percent_Identity=25.9868421052632, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=299, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17559150, Length=257, Percent_Identity=26.8482490272374, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=286, Percent_Identity=25.8741258741259, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): THTR_METTH (O26719)

Other databases:

- EMBL:   AE000666
- PIR:   G69182
- RefSeq:   NP_275765.1
- ProteinModelPortal:   O26719
- STRING:   O26719
- GeneID:   1470583
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH622
- NMPDR:   fig|187420.1.peg.620
- eggNOG:   arNOG06368
- HOGENOM:   HBG709927
- OMA:   GHENILV
- PhylomeDB:   O26719
- ProtClustDB:   CLSK700129
- BioCyc:   MTHE187420:MTH622-MONOMER
- BRENDA:   2.8.1.1
- InterPro:   IPR001763
- InterPro:   IPR001307
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 32166; Mature: 32166

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3

Important sites: ACT_SITE 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPYGKGDGRVRWVTPEWLMENMEDVAIIDCQPNIHDYILEHIPGAVYLNEGLFREPRGK
CCCCCCCCCEEEEECHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCC
APAMYIPEGAVELIFQQAGIENRPTVVYTGTGGVKGWGDGLEQTMVAYSLARFGHENILV
CCEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
LNGGLAEWKRAGGELTKVFPEVEESGFSAVTKEDFYIEYPEFKRIKDDEDVLLLDARPAE
EECCHHHHHHCCCHHHHHHHHHHHCCCCEEECCCEEEECCHHCCCCCCCCEEEEECCCHH
VYEGQGPWIKPGHIPGAVNLPWADLMDPENRTLLKPEDEILELVNSVGATPDRKIICSCG
HCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEECC
TGREATNEFLLFRWYLGYPDVRIYEGSFTEWTQIEDNPTVTGPDPR
CCCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MEPYGKGDGRVRWVTPEWLMENMEDVAIIDCQPNIHDYILEHIPGAVYLNEGLFREPRGK
CCCCCCCCCEEEEECHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCC
APAMYIPEGAVELIFQQAGIENRPTVVYTGTGGVKGWGDGLEQTMVAYSLARFGHENILV
CCEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
LNGGLAEWKRAGGELTKVFPEVEESGFSAVTKEDFYIEYPEFKRIKDDEDVLLLDARPAE
EECCHHHHHHCCCHHHHHHHHHHHCCCCEEECCCEEEECCHHCCCCCCCCEEEEECCCHH
VYEGQGPWIKPGHIPGAVNLPWADLMDPENRTLLKPEDEILELVNSVGATPDRKIICSCG
HCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEECC
TGREATNEFLLFRWYLGYPDVRIYEGSFTEWTQIEDNPTVTGPDPR
CCCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463