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The map label for this gene is pyrG

Identifier: 15678447

GI number: 15678447

Start: 355243

End: 356844

Strand: Direct

Name: pyrG

Synonym: MTH419

Alternate gene names: 15678447

Gene position: 355243-356844 (Clockwise)

Preceding gene: 15678446

Following gene: 15678449

Centisome position: 20.28

GC content: 51.94

Gene sequence:

>1602_bases
TTGGTTCATCTGGCCAAGTACATCGTTGTAACGGGAGGAGTTGTAAGCTCTATTGGAAAGGGAATAACAGCAGCATCAAT
AGGCAGGATACTCAGATCATATGGATTATCTGTGACAGCCATAAAGATAGACCCCTACCTGAACTGGGACTCAGGGACCC
TCAACCCCTACCAGCACGGTGAGGTCTTTGTAACCGATGATGGAATGGAGACAGACCTTGACCTGGGCCACTATGAGAGG
TTCCTGGACTCTGATCTCCCTGGTGAGGCCAACATAACAACCGGAAAGGTCTACATGTCCGTCATAAATAAGGAGCGGTC
AGGGGATTACCTGGGCTCCTGTGTACAGATAATACCCCACATAACCGACGAGATCAAGTCAATGATAAGAAAAATCGCTG
ATAAAAGCGGTGCTGAGGTGGTACTGGTGGAGGTCGGGGGTACGGTGGGTGACATAGAGGGTCAGCCCTTCCTGGAGGCC
CTCAGGCAGCTGAGGAACGAGGAGGGTCATGAGAACGTCATGTTCGTCCATGTGACCTACGTCCCATATCTGAGGGCTGC
AGGGGAATTTAAGACCAAACCGACACAGCACAGTACAAAGGAGCTCAGAAGTACAGGTATAAACCCTGATATGATCATCT
GCCGGAGTGAGATGCCAATAGACTCCTCACTCAAGAGGAAGATAGCCCACTTCTGTGACGTTGAGGAGGAGGCCGTTGTG
AATGCCCCCGACGCATCATCAATCTATGAGGTCCCCCTGGTACTTGACAGTGAGCGGGTGGGGGACTACATAGTCCGGAG
GATAGAACTGGATGTTGATGGTGAAGCAGACCTCAGTGAATGGGCAGGGATAGTTGAGTCACTCATGATAGATGAGCCGG
TGGTTACCGTTGGAATAGTCGGCAAGTACGTGGAGCTTGAGGACTCATACATAAGTATAAGGGAAGCCCTCAAACATGCA
GCGGCACACCTACGGGTAAGGGTTGATATTGAGTGGATAAGTGCCGATGATGCAGTAAATGAGGAGGACCTTTCACGTCT
TGACTCCATACTCATCCCTGGTGGATTTGGTGAAAGGGGAATCGCAGGAAAACTTGAGGCGGTCAGGTTCGCCCTTGAAA
ACAGGGTTCCCATCTTCGGCATATGCCTGGGCATGCAGTGCATGGTCATAGAATTCGCAAGGCTCAACGGCATGGAGGGT
GCCAACAGCACAGAGTTCGACCCGGAAACCCCCTACCCTGTCATAGACATGATGGAGGAGCAGAAGAGGATAAAGAACAT
GGGGGGTACCATGCGCCTCGGCTCCTACCAGTGCAGGATAAGGGAGGGTACACTGGCACATGAGGCCTACGGCACCGAAC
TCGTGGGTGAGAGGCACAGGCACAGGTTTGAACTCAACAACGAATTCAGGGAGGAACTGGAGAGTAAGGGCCTTATAATC
TCAGGAACATCTCCGGACGACTTCCTCGTGGAGATGGTTGAGATCAAGGATCACCCATGGTTCCTCGGATGCCAGTTCCA
CCCAGAGTTCAGGTCAAGACCAAACAGGGCCCATCCACTCTTTGTATCCTTCCTCAGGGCCGCCCTTGAAAGGAGCCGTT
AA

Upstream 100 bases:

>100_bases
CAGGCTCATGGAAATCATGATGGACCCGGCTGCCCGGTTAAGGCAATGAGTTTTTAACATCCTAATCACATATATTGACC
CATCAAACTAATCTGAGGAG

Downstream 100 bases:

>100_bases
TAAAGCGGCAAAATCTTTTTTTTATCTACCGTTACATCTTTAGCAGGAAAAAAAGCTCAACTACTTTTTATCAGGTATTC
CAATCCCTGATGGGCTCCCA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MVHLAKYIVVTGGVVSSIGKGITAASIGRILRSYGLSVTAIKIDPYLNWDSGTLNPYQHGEVFVTDDGMETDLDLGHYER
FLDSDLPGEANITTGKVYMSVINKERSGDYLGSCVQIIPHITDEIKSMIRKIADKSGAEVVLVEVGGTVGDIEGQPFLEA
LRQLRNEEGHENVMFVHVTYVPYLRAAGEFKTKPTQHSTKELRSTGINPDMIICRSEMPIDSSLKRKIAHFCDVEEEAVV
NAPDASSIYEVPLVLDSERVGDYIVRRIELDVDGEADLSEWAGIVESLMIDEPVVTVGIVGKYVELEDSYISIREALKHA
AAHLRVRVDIEWISADDAVNEEDLSRLDSILIPGGFGERGIAGKLEAVRFALENRVPIFGICLGMQCMVIEFARLNGMEG
ANSTEFDPETPYPVIDMMEEQKRIKNMGGTMRLGSYQCRIREGTLAHEAYGTELVGERHRHRFELNNEFREELESKGLII
SGTSPDDFLVEMVEIKDHPWFLGCQFHPEFRSRPNRAHPLFVSFLRAALERSR

Sequences:

>Translated_533_residues
MVHLAKYIVVTGGVVSSIGKGITAASIGRILRSYGLSVTAIKIDPYLNWDSGTLNPYQHGEVFVTDDGMETDLDLGHYER
FLDSDLPGEANITTGKVYMSVINKERSGDYLGSCVQIIPHITDEIKSMIRKIADKSGAEVVLVEVGGTVGDIEGQPFLEA
LRQLRNEEGHENVMFVHVTYVPYLRAAGEFKTKPTQHSTKELRSTGINPDMIICRSEMPIDSSLKRKIAHFCDVEEEAVV
NAPDASSIYEVPLVLDSERVGDYIVRRIELDVDGEADLSEWAGIVESLMIDEPVVTVGIVGKYVELEDSYISIREALKHA
AAHLRVRVDIEWISADDAVNEEDLSRLDSILIPGGFGERGIAGKLEAVRFALENRVPIFGICLGMQCMVIEFARLNGMEG
ANSTEFDPETPYPVIDMMEEQKRIKNMGGTMRLGSYQCRIREGTLAHEAYGTELVGERHRHRFELNNEFREELESKGLII
SGTSPDDFLVEMVEIKDHPWFLGCQFHPEFRSRPNRAHPLFVSFLRAALERSR
>Mature_533_residues
MVHLAKYIVVTGGVVSSIGKGITAASIGRILRSYGLSVTAIKIDPYLNWDSGTLNPYQHGEVFVTDDGMETDLDLGHYER
FLDSDLPGEANITTGKVYMSVINKERSGDYLGSCVQIIPHITDEIKSMIRKIADKSGAEVVLVEVGGTVGDIEGQPFLEA
LRQLRNEEGHENVMFVHVTYVPYLRAAGEFKTKPTQHSTKELRSTGINPDMIICRSEMPIDSSLKRKIAHFCDVEEEAVV
NAPDASSIYEVPLVLDSERVGDYIVRRIELDVDGEADLSEWAGIVESLMIDEPVVTVGIVGKYVELEDSYISIREALKHA
AAHLRVRVDIEWISADDAVNEEDLSRLDSILIPGGFGERGIAGKLEAVRFALENRVPIFGICLGMQCMVIEFARLNGMEG
ANSTEFDPETPYPVIDMMEEQKRIKNMGGTMRLGSYQCRIREGTLAHEAYGTELVGERHRHRFELNNEFREELESKGLII
SGTSPDDFLVEMVEIKDHPWFLGCQFHPEFRSRPNRAHPLFVSFLRAALERSR

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=550, Percent_Identity=46.1818181818182, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI28559085, Length=549, Percent_Identity=45.9016393442623, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI28559083, Length=549, Percent_Identity=45.9016393442623, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI221316689, Length=549, Percent_Identity=45.9016393442623, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI1789142, Length=541, Percent_Identity=51.3863216266174, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25148299, Length=600, Percent_Identity=40, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=559, Percent_Identity=43.8282647584973, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322563, Length=561, Percent_Identity=44.0285204991087, Blast_Score=435, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=551, Percent_Identity=45.0090744101633, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI21357815, Length=508, Percent_Identity=42.3228346456693, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_METTH (O26519)

Other databases:

- EMBL:   AE000666
- PIR:   E69154
- RefSeq:   NP_275562.1
- ProteinModelPortal:   O26519
- SMR:   O26519
- STRING:   O26519
- GeneID:   1470380
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH419
- NMPDR:   fig|187420.1.peg.417
- eggNOG:   arNOG04675
- HOGENOM:   HBG597806
- OMA:   KIAHFCD
- ProtClustDB:   PRK05380
- BioCyc:   MTHE187420:MTH419-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59565; Mature: 59565

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 382-382 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHLAKYIVVTGGVVSSIGKGITAASIGRILRSYGLSVTAIKIDPYLNWDSGTLNPYQHG
CCEEEEEHEEECHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCEECCCCCCCCCCCCC
EVFVTDDGMETDLDLGHYERFLDSDLPGEANITTGKVYMSVINKERSGDYLGSCVQIIPH
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITDEIKSMIRKIADKSGAEVVLVEVGGTVGDIEGQPFLEALRQLRNEEGHENVMFVHVTY
HHHHHHHHHHHHHCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHCCCCCCCEEEEEEEE
VPYLRAAGEFKTKPTQHSTKELRSTGINPDMIICRSEMPIDSSLKRKIAHFCDVEEEAVV
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHEE
NAPDASSIYEVPLVLDSERVGDYIVRRIELDVDGEADLSEWAGIVESLMIDEPVVTVGIV
CCCCCCCEEEEEEEECCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
GKYVELEDSYISIREALKHAAAHLRVRVDIEWISADDAVNEEDLSRLDSILIPGGFGERG
EEEEEECHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHEECCCCCCCCC
IAGKLEAVRFALENRVPIFGICLGMQCMVIEFARLNGMEGANSTEFDPETPYPVIDMMEE
CCCHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QKRIKNMGGTMRLGSYQCRIREGTLAHEAYGTELVGERHRHRFELNNEFREELESKGLII
HHHHHHCCCCEEECCEEEEEECCCCHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHCCEEE
SGTSPDDFLVEMVEIKDHPWFLGCQFHPEFRSRPNRAHPLFVSFLRAALERSR
ECCCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVHLAKYIVVTGGVVSSIGKGITAASIGRILRSYGLSVTAIKIDPYLNWDSGTLNPYQHG
CCEEEEEHEEECHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCEECCCCCCCCCCCCC
EVFVTDDGMETDLDLGHYERFLDSDLPGEANITTGKVYMSVINKERSGDYLGSCVQIIPH
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITDEIKSMIRKIADKSGAEVVLVEVGGTVGDIEGQPFLEALRQLRNEEGHENVMFVHVTY
HHHHHHHHHHHHHCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHCCCCCCCEEEEEEEE
VPYLRAAGEFKTKPTQHSTKELRSTGINPDMIICRSEMPIDSSLKRKIAHFCDVEEEAVV
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHEE
NAPDASSIYEVPLVLDSERVGDYIVRRIELDVDGEADLSEWAGIVESLMIDEPVVTVGIV
CCCCCCCEEEEEEEECCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
GKYVELEDSYISIREALKHAAAHLRVRVDIEWISADDAVNEEDLSRLDSILIPGGFGERG
EEEEEECHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHEECCCCCCCCC
IAGKLEAVRFALENRVPIFGICLGMQCMVIEFARLNGMEGANSTEFDPETPYPVIDMMEE
CCCHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QKRIKNMGGTMRLGSYQCRIREGTLAHEAYGTELVGERHRHRFELNNEFREELESKGLII
HHHHHHCCCCEEECCEEEEEECCCCHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHCCEEE
SGTSPDDFLVEMVEIKDHPWFLGCQFHPEFRSRPNRAHPLFVSFLRAALERSR
ECCCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463