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Identifier: 15678361

GI number: 15678361

Start: 268722

End: 269768

Strand: Direct

Name: Not Available

Synonym: MTH333

Alternate gene names: 15678361

Gene position: 268722-269768 (Clockwise)

Preceding gene: 15678360

Following gene: 15678362

Centisome position: 15.34

GC content: 41.45

Gene sequence:

>1047_bases
ATGAAGAGTGCTTTGATTACAGGAATAACTGGTCAGGATGGGGCTTATCTGGCAAAATTTCTCTTGGAAAAGGGCTATGA
GGTTTATGGAATTTACAGAAGGCTTTCAACTCCTAATTTCTGGAGACTTCAGTACCTTGAAATATTTGATAGAATTAATC
TCGTACCCGCAGATCTGACTGATGAGTTCTCACTTCTAGAATCACTTAAAATATCTGATGCAGATGAAGTATACCATCTT
GCTGCACAGAGCTTTGTTGGAACATCATTCGAACAGCCTACGAGTACAGCACATGTTACCGGTGTTGCTGTTACAAGCAT
GCTTGAAGCAATAAGACATTATAATCCCCATATAAGATTCTATCAAGCCTCAAGCAGTGAGATATATGGGGATGGTCACA
CCACAATCCTTAATGAAAACTCTCCATTTAAGCCATCGAGTCCCTATGCAGCTGCAAAACTTTATGGGTACTGGATGACC
AGAATCTACAGAGAAGGATACGACATTTTTGCTTGTAATGGAATACTCTTTAACCATGAAAGTCCGCTACGTGGCCTTGA
GTTTGTCACAAGGAAAATTTCGAATACCGCTGCAAAAATAGCCCTTGGACTTGAAGATGAGCTCCTTCTCGGAAATCTTG
ATGCGAAGAGGGACTGGGGTTATGCACCTGATTATGTTGAGGCTATGCACATGATGCTCCAGCACAAAGAGCCTGATGAC
TTCGTAATAGCTACAGCAGAGACTCATACTGTTAGGGAGTTCTGTGAAAAAAGTTTCGAGGAACTTGGTCTCGACTGGCA
GGATTATGTTAAAGTGGACAAGAGATTTTTCAGACCTCTTGATGTTAATTATCTCTGTGGTGATTACTCAAAGGCCAGAG
AAAACCTTGGTTGGCAACCTAAAACCAAATTTGAAGAACTTGTGAAGATCATGGTTAGAGAGGATCTTGAAAGATGGGAA
AGGTGGCAGAACGGAGAAAAATTCCCCTGGGACGCGTCAAATTATCCTGATGAAACAAAATTGATAACGAGGGCACTGAG
ATCATGA

Upstream 100 bases:

>100_bases
AACATGAAAAATTAAGAAAAGACTGTATAAAATTCGCAGAGAGTTATAGCTGGGATAAAATTGCTGATTTAACTGAAAAT
GTTTATTTAGAGGTTTTAAA

Downstream 100 bases:

>100_bases
TACCTATTCTAGAACCATCTATTGGAAATGAAGAGTTAGAAAATGTCCTGGAAGCTCTAAAGAGCGGATGGATAAGTTCG
AGGGGAGAATTTATAACTAA

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKSALITGITGQDGAYLAKFLLEKGYEVYGIYRRLSTPNFWRLQYLEIFDRINLVPADLTDEFSLLESLKISDADEVYHL
AAQSFVGTSFEQPTSTAHVTGVAVTSMLEAIRHYNPHIRFYQASSSEIYGDGHTTILNENSPFKPSSPYAAAKLYGYWMT
RIYREGYDIFACNGILFNHESPLRGLEFVTRKISNTAAKIALGLEDELLLGNLDAKRDWGYAPDYVEAMHMMLQHKEPDD
FVIATAETHTVREFCEKSFEELGLDWQDYVKVDKRFFRPLDVNYLCGDYSKARENLGWQPKTKFEELVKIMVREDLERWE
RWQNGEKFPWDASNYPDETKLITRALRS

Sequences:

>Translated_348_residues
MKSALITGITGQDGAYLAKFLLEKGYEVYGIYRRLSTPNFWRLQYLEIFDRINLVPADLTDEFSLLESLKISDADEVYHL
AAQSFVGTSFEQPTSTAHVTGVAVTSMLEAIRHYNPHIRFYQASSSEIYGDGHTTILNENSPFKPSSPYAAAKLYGYWMT
RIYREGYDIFACNGILFNHESPLRGLEFVTRKISNTAAKIALGLEDELLLGNLDAKRDWGYAPDYVEAMHMMLQHKEPDD
FVIATAETHTVREFCEKSFEELGLDWQDYVKVDKRFFRPLDVNYLCGDYSKARENLGWQPKTKFEELVKIMVREDLERWE
RWQNGEKFPWDASNYPDETKLITRALRS
>Mature_348_residues
MKSALITGITGQDGAYLAKFLLEKGYEVYGIYRRLSTPNFWRLQYLEIFDRINLVPADLTDEFSLLESLKISDADEVYHL
AAQSFVGTSFEQPTSTAHVTGVAVTSMLEAIRHYNPHIRFYQASSSEIYGDGHTTILNENSPFKPSSPYAAAKLYGYWMT
RIYREGYDIFACNGILFNHESPLRGLEFVTRKISNTAAKIALGLEDELLLGNLDAKRDWGYAPDYVEAMHMMLQHKEPDD
FVIATAETHTVREFCEKSFEELGLDWQDYVKVDKRFFRPLDVNYLCGDYSKARENLGWQPKTKFEELVKIMVREDLERWE
RWQNGEKFPWDASNYPDETKLITRALRS

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=49.8525073746313, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=23.6686390532544, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=47.1428571428571, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=26.4957264957265, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=48.2352941176471, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=48.2352941176471, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=48.2352941176471, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=48.2352941176471, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=48.2352941176471, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=47.9651162790698, Blast_Score=304, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI115532424, Length=282, Percent_Identity=24.113475177305, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24158427, Length=341, Percent_Identity=48.9736070381232, Blast_Score=312, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 40267; Mature: 40267

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSALITGITGQDGAYLAKFLLEKGYEVYGIYRRLSTPNFWRLQYLEIFDRINLVPADLT
CCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
DEFSLLESLKISDADEVYHLAAQSFVGTSFEQPTSTAHVTGVAVTSMLEAIRHYNPHIRF
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
YQASSSEIYGDGHTTILNENSPFKPSSPYAAAKLYGYWMTRIYREGYDIFACNGILFNHE
EECCCCCEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCC
SPLRGLEFVTRKISNTAAKIALGLEDELLLGNLDAKRDWGYAPDYVEAMHMMLQHKEPDD
CCHHHHHHHHHHHHHHHHHEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
FVIATAETHTVREFCEKSFEELGLDWQDYVKVDKRFFRPLDVNYLCGDYSKARENLGWQP
EEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCC
KTKFEELVKIMVREDLERWERWQNGEKFPWDASNYPDETKLITRALRS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKSALITGITGQDGAYLAKFLLEKGYEVYGIYRRLSTPNFWRLQYLEIFDRINLVPADLT
CCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
DEFSLLESLKISDADEVYHLAAQSFVGTSFEQPTSTAHVTGVAVTSMLEAIRHYNPHIRF
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
YQASSSEIYGDGHTTILNENSPFKPSSPYAAAKLYGYWMTRIYREGYDIFACNGILFNHE
EECCCCCEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCC
SPLRGLEFVTRKISNTAAKIALGLEDELLLGNLDAKRDWGYAPDYVEAMHMMLQHKEPDD
CCHHHHHHHHHHHHHHHHHEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
FVIATAETHTVREFCEKSFEELGLDWQDYVKVDKRFFRPLDVNYLCGDYSKARENLGWQP
EEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCC
KTKFEELVKIMVREDLERWERWQNGEKFPWDASNYPDETKLITRALRS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]