Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 15678272

GI number: 15678272

Start: 187995

End: 189329

Strand: Direct

Name: hisS

Synonym: MTH244

Alternate gene names: 15678272

Gene position: 187995-189329 (Clockwise)

Preceding gene: 15678271

Following gene: 15678273

Centisome position: 10.73

GC content: 52.88

Gene sequence:

>1335_bases
ATGAATGATGAACCTCTCATCATCCCCTTTATCATTTTTACTGGAGGAATTGCTGTCATGGATATATCAAGACCGAGAGG
AACAAGGGACTTCCTTTTCGCTGAAATGAGGAACAGGAAAGAGGTTGAAGGTGTACTCAGAAGGACATTCGAGACCTATG
GCTACCATGAGATCAAGACACCAATATTTGAGGAACTCAAACTCTTCACAGTCAAATCCGGTGAGGAGGTGGTGAACCAG
ATCTACCACTTCACCGATAAGGGCGGCCGGGAACTGGCCCTGAGACCTGAACTCACAGCACCCGTGGCAAGGCTCTACAT
GAATGAGATGCAGAAAAAACCGAAGCCAATAAAGATGTACTACTTCGGGAGCTGCTTCAGGTATGAGAGGCCGCAGGCCG
GGCGCTTCAGACAGTTCTGGCAGTTCGGATGCGAACTCATAGGTGGAAGATCGCCCCTGGCAGAGGCTGAGGTGATATCC
CTAGCATCTGAATCCCTCCATGAACTCGGCCTTGACGGATTCGAGGTCCACATAGGCCACCTGGGCATACTCCGCGGGAT
CCTCGGCAGGGAGGAAATCCCATCTGAACTCCAGGACAGGATAATGGGTGTCATAGACAAGGGCGACGTGGAAGAACTTG
AAGCACTCCTAGGGGAGGTTGAGGTATCTGGAGAAAGCCGGGAACTCCTCATGAAGCTCATAGGTACACAGGGGGGCCCG
GAGGTTCTGGACGATGTTGAAGAATACCTGGGGGGCTACAGTGAATCCCTGGAGGCCCTTGAAGAGTTCAGGGAACTCGT
TGAGACCCTCCACTACTTTGGCGTGGATGACTACCATGTGAACCTGGGGATAGCAAGGGGCCTTGACTACTACACAGGGA
TAGTATTCGAGATCTACGTGCCGGAACTGGGGGCCCAGAAACAGATATGTGGCGGAGGGACCTACAACCTGGTTGAAACA
TTCGGTGGGGAGCGGGTCGAATCAACGGGATTCGCCTTCGGCTTTGACAGGCTCATGAACGCCCTGGGGATGGATGAGGA
GAACATGAGGATGGTTGATGTCTTCGTGATACCCATACAGGACTCCACTGTCCCTGAGGCCATAAGGATCACCCAGGAGC
TCAGGGCTGCAGGGGTATCCGCTGACCTCGAAGTCTCTGGCAGGAAGCTCAGGAAGGCCCTCTCACACGCTGACAACCTT
GGAGCCAGGTTCGCAGTTCTGACAGGTGAGAGGGAAATCCAGGATGGTAAGGTGACGGTAAAGGATATGGAGAGGAACTC
CCAGGAGACCCTGCCAAGGGATGAGATGGTTGATAAAATCAAAGGGAGATGTTAA

Upstream 100 bases:

>100_bases
GCGGCCGATGATGGTATAAGGGTCTGGGCTGAGCGACTGGGTCTGAGGTTCCTCAATGCCACATCCTTCCCCAAGATGCT
CAAGGAATACCTTAAATATT

Downstream 100 bases:

>100_bases
CATTTTACTTAATTTCAGACATAATATTAATGGCGAAGACCTCATAATTGCAGTTGCACAGGACCACGAGACAGGTGAGG
TCCTCATGGTGGCCTACATG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNDEPLIIPFIIFTGGIAVMDISRPRGTRDFLFAEMRNRKEVEGVLRRTFETYGYHEIKTPIFEELKLFTVKSGEEVVNQ
IYHFTDKGGRELALRPELTAPVARLYMNEMQKKPKPIKMYYFGSCFRYERPQAGRFRQFWQFGCELIGGRSPLAEAEVIS
LASESLHELGLDGFEVHIGHLGILRGILGREEIPSELQDRIMGVIDKGDVEELEALLGEVEVSGESRELLMKLIGTQGGP
EVLDDVEEYLGGYSESLEALEEFRELVETLHYFGVDDYHVNLGIARGLDYYTGIVFEIYVPELGAQKQICGGGTYNLVET
FGGERVESTGFAFGFDRLMNALGMDEENMRMVDVFVIPIQDSTVPEAIRITQELRAAGVSADLEVSGRKLRKALSHADNL
GARFAVLTGEREIQDGKVTVKDMERNSQETLPRDEMVDKIKGRC

Sequences:

>Translated_444_residues
MNDEPLIIPFIIFTGGIAVMDISRPRGTRDFLFAEMRNRKEVEGVLRRTFETYGYHEIKTPIFEELKLFTVKSGEEVVNQ
IYHFTDKGGRELALRPELTAPVARLYMNEMQKKPKPIKMYYFGSCFRYERPQAGRFRQFWQFGCELIGGRSPLAEAEVIS
LASESLHELGLDGFEVHIGHLGILRGILGREEIPSELQDRIMGVIDKGDVEELEALLGEVEVSGESRELLMKLIGTQGGP
EVLDDVEEYLGGYSESLEALEEFRELVETLHYFGVDDYHVNLGIARGLDYYTGIVFEIYVPELGAQKQICGGGTYNLVET
FGGERVESTGFAFGFDRLMNALGMDEENMRMVDVFVIPIQDSTVPEAIRITQELRAAGVSADLEVSGRKLRKALSHADNL
GARFAVLTGEREIQDGKVTVKDMERNSQETLPRDEMVDKIKGRC
>Mature_444_residues
MNDEPLIIPFIIFTGGIAVMDISRPRGTRDFLFAEMRNRKEVEGVLRRTFETYGYHEIKTPIFEELKLFTVKSGEEVVNQ
IYHFTDKGGRELALRPELTAPVARLYMNEMQKKPKPIKMYYFGSCFRYERPQAGRFRQFWQFGCELIGGRSPLAEAEVIS
LASESLHELGLDGFEVHIGHLGILRGILGREEIPSELQDRIMGVIDKGDVEELEALLGEVEVSGESRELLMKLIGTQGGP
EVLDDVEEYLGGYSESLEALEEFRELVETLHYFGVDDYHVNLGIARGLDYYTGIVFEIYVPELGAQKQICGGGTYNLVET
FGGERVESTGFAFGFDRLMNALGMDEENMRMVDVFVIPIQDSTVPEAIRITQELRAAGVSADLEVSGRKLRKALSHADNL
GARFAVLTGEREIQDGKVTVKDMERNSQETLPRDEMVDKIKGRC

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=464, Percent_Identity=29.7413793103448, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI15029520, Length=451, Percent_Identity=27.7161862527716, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788861, Length=433, Percent_Identity=32.3325635103926, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71993693, Length=454, Percent_Identity=27.5330396475771, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71993686, Length=454, Percent_Identity=27.5330396475771, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325290, Length=454, Percent_Identity=27.3127753303965, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24643061, Length=464, Percent_Identity=25.8620689655172, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24643059, Length=483, Percent_Identity=24.8447204968944, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50063; Mature: 50063

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEPLIIPFIIFTGGIAVMDISRPRGTRDFLFAEMRNRKEVEGVLRRTFETYGYHEIKT
CCCCCEEEEHHHHHCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHCC
PIFEELKLFTVKSGEEVVNQIYHFTDKGGRELALRPELTAPVARLYMNEMQKKPKPIKMY
HHHHHHEEEEECCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
YFGSCFRYERPQAGRFRQFWQFGCELIGGRSPLAEAEVISLASESLHELGLDGFEVHIGH
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHH
LGILRGILGREEIPSELQDRIMGVIDKGDVEELEALLGEVEVSGESRELLMKLIGTQGGP
HHHHHHHHCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCH
EVLDDVEEYLGGYSESLEALEEFRELVETLHYFGVDDYHVNLGIARGLDYYTGIVFEIYV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHCEEEEEEC
PELGAQKQICGGGTYNLVETFGGERVESTGFAFGFDRLMNALGMDEENMRMVDVFVIPIQ
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
DSTVPEAIRITQELRAAGVSADLEVSGRKLRKALSHADNLGARFAVLTGEREIQDGKVTV
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEE
KDMERNSQETLPRDEMVDKIKGRC
EHHHCCHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNDEPLIIPFIIFTGGIAVMDISRPRGTRDFLFAEMRNRKEVEGVLRRTFETYGYHEIKT
CCCCCEEEEHHHHHCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHCC
PIFEELKLFTVKSGEEVVNQIYHFTDKGGRELALRPELTAPVARLYMNEMQKKPKPIKMY
HHHHHHEEEEECCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
YFGSCFRYERPQAGRFRQFWQFGCELIGGRSPLAEAEVISLASESLHELGLDGFEVHIGH
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHH
LGILRGILGREEIPSELQDRIMGVIDKGDVEELEALLGEVEVSGESRELLMKLIGTQGGP
HHHHHHHHCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCH
EVLDDVEEYLGGYSESLEALEEFRELVETLHYFGVDDYHVNLGIARGLDYYTGIVFEIYV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHCEEEEEEC
PELGAQKQICGGGTYNLVETFGGERVESTGFAFGFDRLMNALGMDEENMRMVDVFVIPIQ
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
DSTVPEAIRITQELRAAGVSADLEVSGRKLRKALSHADNLGARFAVLTGEREIQDGKVTV
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEE
KDMERNSQETLPRDEMVDKIKGRC
EHHHCCHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]