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Identifier: 15678256

GI number: 15678256

Start: 170626

End: 171888

Strand: Direct

Name: Not Available

Synonym: MTH228

Alternate gene names: 15678256

Gene position: 170626-171888 (Clockwise)

Preceding gene: 15678255

Following gene: 15678259

Centisome position: 9.74

GC content: 53.76

Gene sequence:

>1263_bases
ATGAAGTCAGAGGAACTCTTCAGAAGGGCCAGTAACGTCCTCCCGGGGGGTGTGAGTTCACCTGTGAGGAGGTTCGACCC
CCACCCATTCTTTGCAGCGGGGGGCAGCGGATGCCTGCTTGAAAGCGTGGATGGTGAAAGCTACATTGACTACTGTCTGG
CCTATGGCCCCCTCATACTGGGACACGCCCACCCACGTGTAGTTGAGGCTGTGAATGAGCAGATAAAAAGGGGGACAACC
TACGGTGTTCCCACAGAGGGGGAGATAGAACTTGCAGAGGCCATCATCGAGAGGGTGCCATGCGCTGAGATGGTGAGGTT
CACAAATTCAGGGACTGAGGCCACCATGGCCGCTGTGAGGCTTGCCAGGGCCTTCACAGGGAGGGACAGGATAGTTAAAT
TTGAGGGATCCTATCACGGTGCCCACGACTACGTCCTTGTGAGGCCGGGTTCAGGGGCGGCCACAGCACCTGATTCACCT
GGAATACCAGTGGATACTGTCAGAAACACCCTGACGGTACCATTCAACCATGAGGAGGCAATGGCTGAACTCATCGAGGG
GGCGGGTGAGGATATAGCGTGCATACTGGTGGAACCTGTCATGGGAAACATAGGGTGCATAGAACCTGAAAATGGTTACC
TGCAGTTCCTGAGGGACATAACACGTGAAAATGATATCATCCTCATATTTGATGAGGTGATAACCGGATTCAGGCTTGCA
CCAGGGGGTGCCCAGGAGTACTACAGGGTTGAACCCGACCTTGTAACCCTTGGAAAGATAGTTGGAGGCGGCTTCCCAAT
GGGGGCACTGGCAGGCAGAAGGGAGATAATGGAAAATATATCACCTGCAGGTAACGTCTACCAGGCAGGTACCTTCAACG
GGAACCCTGTATCTGTAACTGCAGGTCGTGAGACCCTCAGACTCCTTGATGGGAGGATGTATTCTGACCTTGAGAGGAAG
GGGTCGACGCTGAGGGCTGGTCTCAGGGACCTCCTCTCTGACCTGGACCTTGAATACCAGGTCACCGGGCCAGCATCAAT
GTTCCAGTTATACTTCACAGGGGAGGAGGTCAGGAACTATGGGGATGCTAAAAAATCCGATACAGTGCTCTTCATGGAGT
ACTTCCATGGCCTGCTTGAGAGGGGGGTATTCATACCACCGTCCCAGTTTGAGTGCTGCTTCATATCGGCTGCCCATGAA
TCAGAGCACATAGAGGCGACCCTTGAAGCTGCCGAGGAGGTTCTCAGCGGCCTTAAAAATTAA

Upstream 100 bases:

>100_bases
TGATAACCAGGGGTCCCAAGGGGGGCACACCAGTCGCAGTGGCGGCTGCAAACGCACTCATAGCATTATCCAAGGATAAA
GAGGTTTAGAGGTTTTAAAA

Downstream 100 bases:

>100_bases
TTAATTTTTTTGACTGAAATTTTCAGAATCAGGTGAATTTTACTGCTATGAAGAAGTAGAGGAAGCATATCACAACAAAT
ATCAGGAGTGCCATGACAGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKSEELFRRASNVLPGGVSSPVRRFDPHPFFAAGGSGCLLESVDGESYIDYCLAYGPLILGHAHPRVVEAVNEQIKRGTT
YGVPTEGEIELAEAIIERVPCAEMVRFTNSGTEATMAAVRLARAFTGRDRIVKFEGSYHGAHDYVLVRPGSGAATAPDSP
GIPVDTVRNTLTVPFNHEEAMAELIEGAGEDIACILVEPVMGNIGCIEPENGYLQFLRDITRENDIILIFDEVITGFRLA
PGGAQEYYRVEPDLVTLGKIVGGGFPMGALAGRREIMENISPAGNVYQAGTFNGNPVSVTAGRETLRLLDGRMYSDLERK
GSTLRAGLRDLLSDLDLEYQVTGPASMFQLYFTGEEVRNYGDAKKSDTVLFMEYFHGLLERGVFIPPSQFECCFISAAHE
SEHIEATLEAAEEVLSGLKN

Sequences:

>Translated_420_residues
MKSEELFRRASNVLPGGVSSPVRRFDPHPFFAAGGSGCLLESVDGESYIDYCLAYGPLILGHAHPRVVEAVNEQIKRGTT
YGVPTEGEIELAEAIIERVPCAEMVRFTNSGTEATMAAVRLARAFTGRDRIVKFEGSYHGAHDYVLVRPGSGAATAPDSP
GIPVDTVRNTLTVPFNHEEAMAELIEGAGEDIACILVEPVMGNIGCIEPENGYLQFLRDITRENDIILIFDEVITGFRLA
PGGAQEYYRVEPDLVTLGKIVGGGFPMGALAGRREIMENISPAGNVYQAGTFNGNPVSVTAGRETLRLLDGRMYSDLERK
GSTLRAGLRDLLSDLDLEYQVTGPASMFQLYFTGEEVRNYGDAKKSDTVLFMEYFHGLLERGVFIPPSQFECCFISAAHE
SEHIEATLEAAEEVLSGLKN
>Mature_420_residues
MKSEELFRRASNVLPGGVSSPVRRFDPHPFFAAGGSGCLLESVDGESYIDYCLAYGPLILGHAHPRVVEAVNEQIKRGTT
YGVPTEGEIELAEAIIERVPCAEMVRFTNSGTEATMAAVRLARAFTGRDRIVKFEGSYHGAHDYVLVRPGSGAATAPDSP
GIPVDTVRNTLTVPFNHEEAMAELIEGAGEDIACILVEPVMGNIGCIEPENGYLQFLRDITRENDIILIFDEVITGFRLA
PGGAQEYYRVEPDLVTLGKIVGGGFPMGALAGRREIMENISPAGNVYQAGTFNGNPVSVTAGRETLRLLDGRMYSDLERK
GSTLRAGLRDLLSDLDLEYQVTGPASMFQLYFTGEEVRNYGDAKKSDTVLFMEYFHGLLERGVFIPPSQFECCFISAAHE
SEHIEATLEAAEEVLSGLKN

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=326, Percent_Identity=27.3006134969325, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI13994255, Length=342, Percent_Identity=26.9005847953216, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI37574042, Length=353, Percent_Identity=26.3456090651558, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI24119277, Length=349, Percent_Identity=26.0744985673352, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI226442709, Length=337, Percent_Identity=26.1127596439169, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI226442705, Length=352, Percent_Identity=25.2840909090909, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI284507298, Length=241, Percent_Identity=27.8008298755187, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=45.2830188679245, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789016, Length=404, Percent_Identity=31.6831683168317, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1788044, Length=295, Percent_Identity=33.2203389830508, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787560, Length=372, Percent_Identity=28.494623655914, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693181, Length=340, Percent_Identity=29.1176470588235, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789759, Length=329, Percent_Identity=27.355623100304, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1786991, Length=331, Percent_Identity=26.2839879154079, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=326, Percent_Identity=28.5276073619632, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25144274, Length=186, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324432, Length=317, Percent_Identity=28.7066246056782, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323470, Length=333, Percent_Identity=25.8258258258258, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357415, Length=329, Percent_Identity=24.9240121580547, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356575, Length=339, Percent_Identity=28.6135693215339, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_METTH (O26330)

Other databases:

- EMBL:   AE000666
- PIR:   C69128
- RefSeq:   NP_275371.1
- ProteinModelPortal:   O26330
- SMR:   O26330
- STRING:   O26330
- GeneID:   1470189
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH228
- NMPDR:   fig|187420.1.peg.226
- eggNOG:   arNOG04972
- HOGENOM:   HBG725944
- OMA:   DITREND
- ProtClustDB:   PRK00062
- BioCyc:   MTHE187420:MTH228-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45727; Mature: 45727

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEELFRRASNVLPGGVSSPVRRFDPHPFFAAGGSGCLLESVDGESYIDYCLAYGPLIL
CCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCCCEEECCCCHHHHHHHHHHCCEEE
GHAHPRVVEAVNEQIKRGTTYGVPTEGEIELAEAIIERVPCAEMVRFTNSGTEATMAAVR
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
LARAFTGRDRIVKFEGSYHGAHDYVLVRPGSGAATAPDSPGIPVDTVRNTLTVPFNHEEA
HHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCEEEECCCCHHH
MAELIEGAGEDIACILVEPVMGNIGCIEPENGYLQFLRDITRENDIILIFDEVITGFRLA
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCEEEC
PGGAQEYYRVEPDLVTLGKIVGGGFPMGALAGRREIMENISPAGNVYQAGTFNGNPVSVT
CCCCCCCEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEE
AGRETLRLLDGRMYSDLERKGSTLRAGLRDLLSDLDLEYQVTGPASMFQLYFTGEEVRNY
CCHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCHHEEEEEEEHHHHHHC
GDAKKSDTVLFMEYFHGLLERGVFIPPSQFECCFISAAHESEHIEATLEAAEEVLSGLKN
CCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSEELFRRASNVLPGGVSSPVRRFDPHPFFAAGGSGCLLESVDGESYIDYCLAYGPLIL
CCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCCCEEECCCCHHHHHHHHHHCCEEE
GHAHPRVVEAVNEQIKRGTTYGVPTEGEIELAEAIIERVPCAEMVRFTNSGTEATMAAVR
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
LARAFTGRDRIVKFEGSYHGAHDYVLVRPGSGAATAPDSPGIPVDTVRNTLTVPFNHEEA
HHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCEEEECCCCHHH
MAELIEGAGEDIACILVEPVMGNIGCIEPENGYLQFLRDITRENDIILIFDEVITGFRLA
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCEEEC
PGGAQEYYRVEPDLVTLGKIVGGGFPMGALAGRREIMENISPAGNVYQAGTFNGNPVSVT
CCCCCCCEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEE
AGRETLRLLDGRMYSDLERKGSTLRAGLRDLLSDLDLEYQVTGPASMFQLYFTGEEVRNY
CCHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCHHEEEEEEEHHHHHHC
GDAKKSDTVLFMEYFHGLLERGVFIPPSQFECCFISAAHESEHIEATLEAAEEVLSGLKN
CCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463