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Identifier: 15678216

GI number: 15678216

Start: 133127

End: 134104

Strand: Direct

Name: Not Available

Synonym: MTH188

Alternate gene names: 15678216

Gene position: 133127-134104 (Clockwise)

Preceding gene: 15678215

Following gene: 15678217

Centisome position: 7.6

GC content: 50.72

Gene sequence:

>978_bases
TTGAAGGTAAGTATAATTGGGTCAACGGGACGTGTTGGAAGGGCAACGGCCCTCTGTCTTGCAGAGGAGGAGGCTGTGAA
GACCCTCCACCTCATCTCAAGGAAGGAGAGTCTTGAACAGAACCTCGGCGAGGTCCTTGATATGAGCGACGCCCTCGCAG
CCAAGGGTGTTTCAGTCAAGCTGGAAAACTCTGCAGACATTGAAAACGTCTACGGTTCAAGGATAGTGGTGATAACTGCG
GGTGTCCCCAGAACGGCTGATATGGACAGGGATGATCTTGCATTTAAGAACGGCAGGATCGTTGCAGATTATGCAAGGCA
GATCGCAAGATTCGCCCCGGATTCAATTATACTGGTTGTTACAAACCCTGTGGATGTGATGACCTATGTTGCCCTCAGGT
ATTCGGGTTTTCACCCCAGCAGGGTCTTCGGCCTTGGAAACCACCTGGACTCCCTGAGGCTCAAGAACTACATGGCAAGG
CACTTCAATGTCCATGTGAGTGAGGTTCACACCAGGGTTATAGGTCAGCACGGTCCCTACATGGTCCCACTCATAAGTTC
AACCTCCATAGGGGGCATACCCATAGAGCACTATGCCCGGAGGGACTACTTCTCAGGTTACAAGAAGTTTGACCTCAAAA
AGACCATAGACAAGGTCATCCATGCAGGGAGCAACATCATAAGCAGGAAGGGGGCCACCGAGTATGGACCGGCCTTTGCA
ATATCCAACATAGTCACCACCATCCTGAATGATGAACGCAGGATACTCACGGTTTCAACCCTCATGGAGGGTGAAATCGA
TGGTATAAGGGATGTTTGCCTCGGAGTACCTGTTAAACTGGGTAAGAACGGCATTGAGGGGGTTGTACCTGTGCTGATGG
ATCGTGATGAAAGGGAGGCATTCAGGGAAGCCGCCAACCATGTTAGAGACTCCACCAGGAGGGTTATGGAGTTTCTTGAT
GAGGAACTTCCACTATAG

Upstream 100 bases:

>100_bases
AAAGGGTCAGGGCTGCAATGGAGAAACTTGCAGAGACAGGTACCGGTTTTGCAAGGAAGGTGGCAGTTAACTACCTTGAA
ACCCACTAGGAGGAACCCCC

Downstream 100 bases:

>100_bases
TCCGGTTCAGTGTGATGGGGTGAAGGGTTTACCATGGCCGGACCTTCAGTGTGCATTCAACCTCCCTGAGGTCATTTCCT
TCCATCTTTCAGTAACCCCA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKVSIIGSTGRVGRATALCLAEEEAVKTLHLISRKESLEQNLGEVLDMSDALAAKGVSVKLENSADIENVYGSRIVVITA
GVPRTADMDRDDLAFKNGRIVADYARQIARFAPDSIILVVTNPVDVMTYVALRYSGFHPSRVFGLGNHLDSLRLKNYMAR
HFNVHVSEVHTRVIGQHGPYMVPLISSTSIGGIPIEHYARRDYFSGYKKFDLKKTIDKVIHAGSNIISRKGATEYGPAFA
ISNIVTTILNDERRILTVSTLMEGEIDGIRDVCLGVPVKLGKNGIEGVVPVLMDRDEREAFREAANHVRDSTRRVMEFLD
EELPL

Sequences:

>Translated_325_residues
MKVSIIGSTGRVGRATALCLAEEEAVKTLHLISRKESLEQNLGEVLDMSDALAAKGVSVKLENSADIENVYGSRIVVITA
GVPRTADMDRDDLAFKNGRIVADYARQIARFAPDSIILVVTNPVDVMTYVALRYSGFHPSRVFGLGNHLDSLRLKNYMAR
HFNVHVSEVHTRVIGQHGPYMVPLISSTSIGGIPIEHYARRDYFSGYKKFDLKKTIDKVIHAGSNIISRKGATEYGPAFA
ISNIVTTILNDERRILTVSTLMEGEIDGIRDVCLGVPVKLGKNGIEGVVPVLMDRDEREAFREAANHVRDSTRRVMEFLD
EELPL
>Mature_325_residues
MKVSIIGSTGRVGRATALCLAEEEAVKTLHLISRKESLEQNLGEVLDMSDALAAKGVSVKLENSADIENVYGSRIVVITA
GVPRTADMDRDDLAFKNGRIVADYARQIARFAPDSIILVVTNPVDVMTYVALRYSGFHPSRVFGLGNHLDSLRLKNYMAR
HFNVHVSEVHTRVIGQHGPYMVPLISSTSIGGIPIEHYARRDYFSGYKKFDLKKTIDKVIHAGSNIISRKGATEYGPAFA
ISNIVTTILNDERRILTVSTLMEGEIDGIRDVCLGVPVKLGKNGIEGVVPVLMDRDEREAFREAANHVRDSTRRVMEFLD
EELPL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=30.2839116719243, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=30.2839116719243, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=31.1475409836066, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=31.1475409836066, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=29.8701298701299, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=29.8701298701299, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI291575128, Length=311, Percent_Identity=28.2958199356913, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557032, Length=311, Percent_Identity=28.2958199356913, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI15082234, Length=312, Percent_Identity=28.525641025641, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI207028494, Length=189, Percent_Identity=32.2751322751323, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI260099725, Length=197, Percent_Identity=30.9644670050761, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI260099727, Length=197, Percent_Identity=30.9644670050761, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI21735621, Length=296, Percent_Identity=26.0135135135135, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI103472011, Length=315, Percent_Identity=21.5873015873016, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789632, Length=302, Percent_Identity=28.1456953642384, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535107, Length=266, Percent_Identity=31.5789473684211, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17554310, Length=303, Percent_Identity=26.4026402640264, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322765, Length=310, Percent_Identity=30.3225806451613, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=227, Percent_Identity=28.6343612334802, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17136226, Length=312, Percent_Identity=29.8076923076923, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550422, Length=308, Percent_Identity=29.8701298701299, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24647881, Length=334, Percent_Identity=25.748502994012, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663599, Length=258, Percent_Identity=25.968992248062, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24663595, Length=261, Percent_Identity=27.2030651340996, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_METTH (O26290)

Other databases:

- EMBL:   AE000666
- PIR:   E69118
- RefSeq:   NP_275331.1
- ProteinModelPortal:   O26290
- STRING:   O26290
- GeneID:   1470149
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH188
- NMPDR:   fig|187420.1.peg.186
- eggNOG:   arNOG04956
- HOGENOM:   HBG566126
- OMA:   DAMTYVM
- PhylomeDB:   O26290
- ProtClustDB:   PRK06223
- BioCyc:   MetaCyc:MONOMER-14545
- BioCyc:   MTHE187420:MTH188-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35934; Mature: 35934

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: ACT_SITE 177-177 BINDING 84-84 BINDING 90-90 BINDING 97-97 BINDING 122-122 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSIIGSTGRVGRATALCLAEEEAVKTLHLISRKESLEQNLGEVLDMSDALAAKGVSVK
CEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEE
LENSADIENVYGSRIVVITAGVPRTADMDRDDLAFKNGRIVADYARQIARFAPDSIILVV
ECCCCCHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCEEEHHHHHHHHHHCCCCEEEEE
TNPVDVMTYVALRYSGFHPSRVFGLGNHLDSLRLKNYMARHFNVHVSEVHTRVIGQHGPY
ECCHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCE
MVPLISSTSIGGIPIEHYARRDYFSGYKKFDLKKTIDKVIHAGSNIISRKGATEYGPAFA
EEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
ISNIVTTILNDERRILTVSTLMEGEIDGIRDVCLGVPVKLGKNGIEGVVPVLMDRDEREA
HHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHHHCCCHHHCCCCCCHHHHHHCCCCHHHH
FREAANHVRDSTRRVMEFLDEELPL
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSIIGSTGRVGRATALCLAEEEAVKTLHLISRKESLEQNLGEVLDMSDALAAKGVSVK
CEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEE
LENSADIENVYGSRIVVITAGVPRTADMDRDDLAFKNGRIVADYARQIARFAPDSIILVV
ECCCCCHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCEEEHHHHHHHHHHCCCCEEEEE
TNPVDVMTYVALRYSGFHPSRVFGLGNHLDSLRLKNYMARHFNVHVSEVHTRVIGQHGPY
ECCHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCE
MVPLISSTSIGGIPIEHYARRDYFSGYKKFDLKKTIDKVIHAGSNIISRKGATEYGPAFA
EEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
ISNIVTTILNDERRILTVSTLMEGEIDGIRDVCLGVPVKLGKNGIEGVVPVLMDRDEREA
HHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHHHCCCHHHCCCCCCHHHHHHCCCCHHHH
FREAANHVRDSTRRVMEFLDEELPL
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463