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The map label for this gene is rcfB

Identifier: 15674216

GI number: 15674216

Start: 2317694

End: 2318380

Strand: Reverse

Name: rcfB

Synonym: L0245

Alternate gene names: 15674216

Gene position: 2318380-2317694 (Counterclockwise)

Preceding gene: 15674217

Following gene: 15674215

Centisome position: 98.0

GC content: 31.3

Gene sequence:

>687_bases
ATGAGTTTTAATAAGATGGAAGAAATGGCAAACGTAAACATTGAATATCTAATCAATACACTAGAGCAAAAAAAAGTCAG
TGTCGTAACACGTAAAAGGCATAGCTATATTATGTATCAAGGAATTGAGTCAGAATATATCTATGTCCTTAAAGATGGTG
TAGCGAAGATTAGCAATATTTTAAGAGATGGTCGTGAATTTAATATCGCGTATGTTGCGCAGCCAGATTTTGTTTCTTTA
TTGGAAGAAAAACAAAATGATGGAATCTCAGCATTATTTAATGTCAGAGTGGAATCTCCAACGGCTAATTTTTATAAAAT
TTCGCGGAGCGATTTTTGGGATTGGGTGCGCGAAGATTTAAATTTATTTAGAGTTGTTGATGATTTTTATAAAAGAAGAT
TGGCACTTAATTTAGAAATTCTTCAAAAAATGACAATTAATGGTAAAAAAGGAGCAGTTTGTGCTTGTCTCCATAGTTTA
ATTGATGATTTTGGCATTCGTAAGAAAGATGGAATTTTGATTGATTTCACAGTTACTAATGAGGATATTGCAGGCTTCTG
TGGTATTTCTACAAGAAACAGTGTCAATCGTATTCTCCACGATTTAAAGGATGAGAAAGTAATTGATGTGATTGATAATA
AGATCATGATTTATAATCCTCAATATTTGGAAGAATATATTAGTTAA

Upstream 100 bases:

>100_bases
TCAAGTTCTAAAGTTTGATTTTAATGATTTACATAAAAAATGATATAATGAAATGGCTACAGCCTTATATAGGCGAATAA
ATGAACAAATTAGCGAGTAG

Downstream 100 bases:

>100_bases
TATAAACAGATAAAAAAGCTACTTTAGGTAGCTTTTTTGTTATAATTAGGTCAGCATGATATCAAAGTATTCAAGGAGGT
AAAATGGATAATTTAAAAAA

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSFNKMEEMANVNIEYLINTLEQKKVSVVTRKRHSYIMYQGIESEYIYVLKDGVAKISNILRDGREFNIAYVAQPDFVSL
LEEKQNDGISALFNVRVESPTANFYKISRSDFWDWVREDLNLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSL
IDDFGIRKKDGILIDFTVTNEDIAGFCGISTRNSVNRILHDLKDEKVIDVIDNKIMIYNPQYLEEYIS

Sequences:

>Translated_228_residues
MSFNKMEEMANVNIEYLINTLEQKKVSVVTRKRHSYIMYQGIESEYIYVLKDGVAKISNILRDGREFNIAYVAQPDFVSL
LEEKQNDGISALFNVRVESPTANFYKISRSDFWDWVREDLNLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSL
IDDFGIRKKDGILIDFTVTNEDIAGFCGISTRNSVNRILHDLKDEKVIDVIDNKIMIYNPQYLEEYIS
>Mature_227_residues
SFNKMEEMANVNIEYLINTLEQKKVSVVTRKRHSYIMYQGIESEYIYVLKDGVAKISNILRDGREFNIAYVAQPDFVSLL
EEKQNDGISALFNVRVESPTANFYKISRSDFWDWVREDLNLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLI
DDFGIRKKDGILIDFTVTNEDIAGFCGISTRNSVNRILHDLKDEKVIDVIDNKIMIYNPQYLEEYIS

Specific function: It is involved in the activation of genes necessary for anaerobic respiration [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26518; Mature: 26387

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNKMEEMANVNIEYLINTLEQKKVSVVTRKRHSYIMYQGIESEYIYVLKDGVAKISNI
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHHH
LRDGREFNIAYVAQPDFVSLLEEKQNDGISALFNVRVESPTANFYKISRSDFWDWVREDL
HHCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEECHHHHHHHHHHHH
NLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLIDDFGIRKKDGILIDFTVTN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECC
EDIAGFCGISTRNSVNRILHDLKDEKVIDVIDNKIMIYNPQYLEEYIS
CCCCEECCCCCHHHHHHHHHHHCCCHHHHHHCCEEEEECHHHHHHHCC
>Mature Secondary Structure 
SFNKMEEMANVNIEYLINTLEQKKVSVVTRKRHSYIMYQGIESEYIYVLKDGVAKISNI
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHHH
LRDGREFNIAYVAQPDFVSLLEEKQNDGISALFNVRVESPTANFYKISRSDFWDWVREDL
HHCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEECHHHHHHHHHHHH
NLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLIDDFGIRKKDGILIDFTVTN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECC
EDIAGFCGISTRNSVNRILHDLKDEKVIDVIDNKIMIYNPQYLEEYIS
CCCCEECCCCCHHHHHHHHHHHCCCHHHHHHCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377 [H]