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The map label for this gene is asnH

Identifier: 15674210

GI number: 15674210

Start: 2311209

End: 2313086

Strand: Reverse

Name: asnH

Synonym: L0095

Alternate gene names: 15674210

Gene position: 2313086-2311209 (Counterclockwise)

Preceding gene: 15674211

Following gene: 15674209

Centisome position: 97.78

GC content: 35.52

Gene sequence:

>1878_bases
ATGTGTGGAATTGTCGGCTTTATTGACCGGATGAATCAAAATGATAAATCAAAAACTTTAGAAGAAATGATGAATACTAT
TGGTCACCGTGGTCCAAGTAGTTCAGGTGAATTTATTGATGAAGGTGCCGCAATTGGCTTTCGTCGTTTGAGTATTATTG
ACCTTGAAGGTGGAGACCAACCCATTTTCAATGAAGATAAAAGTAAATTGATTACTTTTAACGGTGAAATTTATAACTTC
CGCGAATTACGTGAGGACCTTATCGCTAAAGGTCATGATTTTACAACTCATGCTGATACAGAAGTTCTTTTGCATGGTTA
TGAAGAATATGGTGTAGAACTTCTTCAAAAGCTTCGTGGAATGTTCGCTTTTGTTATTTGGGATAGAGAAAAACAAGAAT
TGTTTGGTGCTCGTGATCATTTTGGTATCAAACCACTTTACTATACTCAAATGGGTGGAACTTTCATGTATGGTTCAGAA
ATTAAAAGTTTTTTGAAACATCCAAACTTCAAAAAAGAATTAAATAAAGAAGCTTTAAAACCTTACATGACTTTCCAATA
CTCTGCTCTTGATGAAACTTTCTTTAAAAATGTTTTTCGTATCAAAGAAGGTCATTACTTTACTTACAAAGATGGGGTCT
TGGATATCAAACCTTATTGGGATATGAAAGTTGATGAAAAAGATATGAGCTTGGAAGAATCAATTGATTTGATTGATAAG
ACTGTTTTGGGTTCTGTTGACGCTCATAAATTTGCTGACGTAGAAGTTGGAGCTTTTGTATCAAGTGGTGTCGATTCTAG
TTATGTTGCTTCAACGCTTCGTCCTGAACATACTTATTCTATTGGTTTTGGTCAAGGAACTTTCAATGAATCTAAACAAG
CCAAACAATTGACTGATATGATTGGTTTGAACAATACTTCTCGTGAAATCCATGGAGATGAAGCGTTCAAGTATTTCCCA
CAAATTCAATATTATTTGGATGAACCAGATTCAAATCCTTCTTGTGTGCCACTCTTCTTCCTCGCAGAATTAGCAGCACG
TGATGTACGTGTTGTAATGAGCGGTGAAGGTGCCGATGAGCTTTTTGCTGGTTATGAGTCTTATGGATTTAGTACAAATT
CTCGTGCGGTTCGTGTTTTCGCAGAAGGTTTGAAGAAATTACCAAAATCTTTACGTTTCTCAATTGCTCGTGGTATTGAA
AATAAACATTTCAAAGGTCAAAAACATCTTTATGAATCACTTGCGCCTGCTGAAGATTTCTTTATCGGTCAAGCGAAAGT
GTTTGATGAGTCTGAATCAGTAGAGCTTTTGAAAGAAGAATTTAAAGCAGCTCCAACTGTTAAAGATATTGTTGGAAAAA
CTTACAAAAAGGCCCCAGAAGGTTCTGAACTTCGTAAAATGCAATATCTTGATATGCACCAATGGATGCCAGGAGATATC
CTGCTTAAAGCTGATAAAATGGCAATGGCACACTCACTTGAATTGCGTGTTCCACTACTTGATATTGAATTGATGCATAC
AGCGGAACAAATTCCTACAAAATATCTTTTGAATGAGCATAATACAAAGTATGCTTTCCGCCAAGCAGCTCGTCGTCATT
TACCAGAAGAATGGTCAAATCGTAAAAAATTAGGCTTCCCAGTGCCAATTAAAGACTGGTTGCGAGAAGAAAAATATTAT
AAAGTTGTTCGTCAATTATTTGAACAAGATTTTGTTGCTGAATTCTTTGATCAAGAAAAACTCTTGAAGATGTTGGATGA
TAACTTTGCAGACAAAAATGATGCACGTCGTAAACTCTGGACAATTTATTCATTCTTGACTTGGTATGATGTTTACTTCA
TCAATAATGGTGAAAAACCTGCTGTGGTTAATTTTTAA

Upstream 100 bases:

>100_bases
AAATAGTGATTTGATAGTGGGGAATAATTTTCCTTCTGTCAAATCATTTTTTATTATTGTGGTATAATAATAAGGAAAAA
TGATAAGGGGATAGATACAA

Downstream 100 bases:

>100_bases
GGGAGGTTTTTGTGTCACAAACAAATGAATTTCAACCTATCTTGTTAGGTTCTGATATCAATGTCTATGGAATGGCTCGT
TCATTTCATGAAGAATTTGG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MCGIVGFIDRMNQNDKSKTLEEMMNTIGHRGPSSSGEFIDEGAAIGFRRLSIIDLEGGDQPIFNEDKSKLITFNGEIYNF
RELREDLIAKGHDFTTHADTEVLLHGYEEYGVELLQKLRGMFAFVIWDREKQELFGARDHFGIKPLYYTQMGGTFMYGSE
IKSFLKHPNFKKELNKEALKPYMTFQYSALDETFFKNVFRIKEGHYFTYKDGVLDIKPYWDMKVDEKDMSLEESIDLIDK
TVLGSVDAHKFADVEVGAFVSSGVDSSYVASTLRPEHTYSIGFGQGTFNESKQAKQLTDMIGLNNTSREIHGDEAFKYFP
QIQYYLDEPDSNPSCVPLFFLAELAARDVRVVMSGEGADELFAGYESYGFSTNSRAVRVFAEGLKKLPKSLRFSIARGIE
NKHFKGQKHLYESLAPAEDFFIGQAKVFDESESVELLKEEFKAAPTVKDIVGKTYKKAPEGSELRKMQYLDMHQWMPGDI
LLKADKMAMAHSLELRVPLLDIELMHTAEQIPTKYLLNEHNTKYAFRQAARRHLPEEWSNRKKLGFPVPIKDWLREEKYY
KVVRQLFEQDFVAEFFDQEKLLKMLDDNFADKNDARRKLWTIYSFLTWYDVYFINNGEKPAVVNF

Sequences:

>Translated_625_residues
MCGIVGFIDRMNQNDKSKTLEEMMNTIGHRGPSSSGEFIDEGAAIGFRRLSIIDLEGGDQPIFNEDKSKLITFNGEIYNF
RELREDLIAKGHDFTTHADTEVLLHGYEEYGVELLQKLRGMFAFVIWDREKQELFGARDHFGIKPLYYTQMGGTFMYGSE
IKSFLKHPNFKKELNKEALKPYMTFQYSALDETFFKNVFRIKEGHYFTYKDGVLDIKPYWDMKVDEKDMSLEESIDLIDK
TVLGSVDAHKFADVEVGAFVSSGVDSSYVASTLRPEHTYSIGFGQGTFNESKQAKQLTDMIGLNNTSREIHGDEAFKYFP
QIQYYLDEPDSNPSCVPLFFLAELAARDVRVVMSGEGADELFAGYESYGFSTNSRAVRVFAEGLKKLPKSLRFSIARGIE
NKHFKGQKHLYESLAPAEDFFIGQAKVFDESESVELLKEEFKAAPTVKDIVGKTYKKAPEGSELRKMQYLDMHQWMPGDI
LLKADKMAMAHSLELRVPLLDIELMHTAEQIPTKYLLNEHNTKYAFRQAARRHLPEEWSNRKKLGFPVPIKDWLREEKYY
KVVRQLFEQDFVAEFFDQEKLLKMLDDNFADKNDARRKLWTIYSFLTWYDVYFINNGEKPAVVNF
>Mature_625_residues
MCGIVGFIDRMNQNDKSKTLEEMMNTIGHRGPSSSGEFIDEGAAIGFRRLSIIDLEGGDQPIFNEDKSKLITFNGEIYNF
RELREDLIAKGHDFTTHADTEVLLHGYEEYGVELLQKLRGMFAFVIWDREKQELFGARDHFGIKPLYYTQMGGTFMYGSE
IKSFLKHPNFKKELNKEALKPYMTFQYSALDETFFKNVFRIKEGHYFTYKDGVLDIKPYWDMKVDEKDMSLEESIDLIDK
TVLGSVDAHKFADVEVGAFVSSGVDSSYVASTLRPEHTYSIGFGQGTFNESKQAKQLTDMIGLNNTSREIHGDEAFKYFP
QIQYYLDEPDSNPSCVPLFFLAELAARDVRVVMSGEGADELFAGYESYGFSTNSRAVRVFAEGLKKLPKSLRFSIARGIE
NKHFKGQKHLYESLAPAEDFFIGQAKVFDESESVELLKEEFKAAPTVKDIVGKTYKKAPEGSELRKMQYLDMHQWMPGDI
LLKADKMAMAHSLELRVPLLDIELMHTAEQIPTKYLLNEHNTKYAFRQAARRHLPEEWSNRKKLGFPVPIKDWLREEKYY
KVVRQLFEQDFVAEFFDQEKLLKMLDDNFADKNDARRKLWTIYSFLTWYDVYFINNGEKPAVVNF

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=407, Percent_Identity=26.5356265356265, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI168229252, Length=407, Percent_Identity=26.5356265356265, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI168229250, Length=407, Percent_Identity=26.5356265356265, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI296010848, Length=381, Percent_Identity=26.7716535433071, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI296010852, Length=311, Percent_Identity=25.7234726688103, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI296010850, Length=311, Percent_Identity=25.7234726688103, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1786889, Length=424, Percent_Identity=28.0660377358491, Blast_Score=151, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=429, Percent_Identity=27.972027972028, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=429, Percent_Identity=27.972027972028, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=319, Percent_Identity=28.5266457680251, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=409, Percent_Identity=26.161369193154, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6325403, Length=410, Percent_Identity=25.609756097561, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45553209, Length=433, Percent_Identity=28.6374133949192, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72235; Mature: 72235

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGFIDRMNQNDKSKTLEEMMNTIGHRGPSSSGEFIDEGAAIGFRRLSIIDLEGGDQ
CCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEEEEEEECCCCC
PIFNEDKSKLITFNGEIYNFRELREDLIAKGHDFTTHADTEVLLHGYEEYGVELLQKLRG
CCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHH
MFAFVIWDREKQELFGARDHFGIKPLYYTQMGGTFMYGSEIKSFLKHPNFKKELNKEALK
HEEEEEECCCHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHHCCCHHHHHCHHHHC
PYMTFQYSALDETFFKNVFRIKEGHYFTYKDGVLDIKPYWDMKVDEKDMSLEESIDLIDK
HHHEEEHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCEECCCCCCHHHHHHHHHH
TVLGSVDAHKFADVEVGAFVSSGVDSSYVASTLRPEHTYSIGFGQGTFNESKQAKQLTDM
HHHCCCCCHHCCCCCHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH
IGLNNTSREIHGDEAFKYFPQIQYYLDEPDSNPSCVPLFFLAELAARDVRVVMSGEGADE
HCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHH
LFAGYESYGFSTNSRAVRVFAEGLKKLPKSLRFSIARGIENKHFKGQKHLYESLAPAEDF
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHH
FIGQAKVFDESESVELLKEEFKAAPTVKDIVGKTYKKAPEGSELRKMQYLDMHQWMPGDI
CCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCE
LLKADKMAMAHSLELRVPLLDIELMHTAEQIPTKYLLNEHNTKYAFRQAARRHLPEEWSN
EEEHHHHHHHHHCEEECEEEHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCHHHCC
RKKLGFPVPIKDWLREEKYYKVVRQLFEQDFVAEFFDQEKLLKMLDDNFADKNDARRKLW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHH
TIYSFLTWYDVYFINNGEKPAVVNF
HHHHHHHHHEEEEEECCCCCCEECC
>Mature Secondary Structure
MCGIVGFIDRMNQNDKSKTLEEMMNTIGHRGPSSSGEFIDEGAAIGFRRLSIIDLEGGDQ
CCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEEEEEEECCCCC
PIFNEDKSKLITFNGEIYNFRELREDLIAKGHDFTTHADTEVLLHGYEEYGVELLQKLRG
CCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHH
MFAFVIWDREKQELFGARDHFGIKPLYYTQMGGTFMYGSEIKSFLKHPNFKKELNKEALK
HEEEEEECCCHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHHCCCHHHHHCHHHHC
PYMTFQYSALDETFFKNVFRIKEGHYFTYKDGVLDIKPYWDMKVDEKDMSLEESIDLIDK
HHHEEEHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCEECCCCCCHHHHHHHHHH
TVLGSVDAHKFADVEVGAFVSSGVDSSYVASTLRPEHTYSIGFGQGTFNESKQAKQLTDM
HHHCCCCCHHCCCCCHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH
IGLNNTSREIHGDEAFKYFPQIQYYLDEPDSNPSCVPLFFLAELAARDVRVVMSGEGADE
HCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHH
LFAGYESYGFSTNSRAVRVFAEGLKKLPKSLRFSIARGIENKHFKGQKHLYESLAPAEDF
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHH
FIGQAKVFDESESVELLKEEFKAAPTVKDIVGKTYKKAPEGSELRKMQYLDMHQWMPGDI
CCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCE
LLKADKMAMAHSLELRVPLLDIELMHTAEQIPTKYLLNEHNTKYAFRQAARRHLPEEWSN
EEEHHHHHHHHHCEEECEEEHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCHHHCC
RKKLGFPVPIKDWLREEKYYKVVRQLFEQDFVAEFFDQEKLLKMLDDNFADKNDARRKLW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHH
TIYSFLTWYDVYFINNGEKPAVVNF
HHHHHHHHHEEEEEECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]