Click here to switch to the map view.

The map label for this gene is ywjF

Identifier: 15674197

GI number: 15674197

Start: 2298168

End: 2299049

Strand: Reverse

Name: ywjF

Synonym: L81616

Alternate gene names: 15674197

Gene position: 2299049-2298168 (Counterclockwise)

Preceding gene: 15674198

Following gene: 15674195

Centisome position: 97.19

GC content: 40.25

Gene sequence:

>882_bases
ATGTCAAAAATAGCATTTATTGGTACGGGTGTGATGGGAGCTGCAATGGCTGGCCATCTTATGGACGCTGGTCATGATTT
AATTGTTTATAATCGGACAAAATCAAAAACGGACGGTCTTGTGGCACGTGGTGCAACTTATGCTGAAAATCCACAAAAGG
CTGCGGCTCAAGCTGAGTTTATGATTACAATTGTTGGTTATCCTAAGGACGTCCGCGAAGTTTATTTTGGTGCAGAAGGA
ATTTTCAAAAATGATTGTTCAGGAAAAGTTCTTATTGATATGACGACGACTGAGCCAACTTTAGCACAAGAAATTTATCA
AGAAGCTAAAAAAGTTGGAGCTTTCGCACTTGATGCCCCTGTTTCTGGAGGAGATTTGGGTGCAAAAAATGCGACACTAA
CCATTATGGTTGGTGGTGATTCAGAAACTTATGAGAAAGCTTTGCCTTTATTTGAAAAAATGGGAAAAACTATTACTCAT
CAAGGGGCGGTTGGAAGTGGACAACACACTAAAATGGCTAACCAAATTTGTATTGCGGGTACAATGACTGGAATGACAGA
GCTTTTAGCTTATGCCCAAAAGGCTGGTTTGGATTTGGATAAAGTTTTAAATACTGTTGGGGGAGGTTCTGGTGCCACTT
GGTCTTTGGCTAATTACGGTCCTCGGATTCTCCGTGAAGATTATAGTGCAGGATTTTTTGTTAAGCATTTCATCAAAGAC
TTAGGGATTGCACTTTCTGAGGCAGAAAAAATGAATCTTAAGCTTCCTGCAACTAAAGGGGCAAAGGAACTCTATGAGCA
ATTAGCTGATAAAGGCTTTGAAAATGATGGGACTCAAGCTTTGATTAAACTTTGGTGGCCAAATGGACATCGTCCAGAAT
AA

Upstream 100 bases:

>100_bases
GAAAAATCAAACGGTTGCGGGGAGATAAAGCCCGTAAACGTCGTGATTACCAAAATTAGACGAAACAGATTTTATAAATC
TGCTAAATAAAGGAGAAGAA

Downstream 100 bases:

>100_bases
TAATTTCAAAAAAATCCAATAGTTGGTTAAACTATTGGATTTTATATTAAGTTTCATTTCTTACCTTGCTTAGGATTGGG
AGTTGAACGTCTATGCGTTT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGATYAENPQKAAAQAEFMITIVGYPKDVREVYFGAEG
IFKNDCSGKVLIDMTTTEPTLAQEIYQEAKKVGAFALDAPVSGGDLGAKNATLTIMVGGDSETYEKALPLFEKMGKTITH
QGAVGSGQHTKMANQICIAGTMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
LGIALSEAEKMNLKLPATKGAKELYEQLADKGFENDGTQALIKLWWPNGHRPE

Sequences:

>Translated_293_residues
MSKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGATYAENPQKAAAQAEFMITIVGYPKDVREVYFGAEG
IFKNDCSGKVLIDMTTTEPTLAQEIYQEAKKVGAFALDAPVSGGDLGAKNATLTIMVGGDSETYEKALPLFEKMGKTITH
QGAVGSGQHTKMANQICIAGTMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
LGIALSEAEKMNLKLPATKGAKELYEQLADKGFENDGTQALIKLWWPNGHRPE
>Mature_292_residues
SKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGATYAENPQKAAAQAEFMITIVGYPKDVREVYFGAEGI
FKNDCSGKVLIDMTTTEPTLAQEIYQEAKKVGAFALDAPVSGGDLGAKNATLTIMVGGDSETYEKALPLFEKMGKTITHQ
GAVGSGQHTKMANQICIAGTMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKDL
GIALSEAEKMNLKLPATKGAKELYEQLADKGFENDGTQALIKLWWPNGHRPE

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=283, Percent_Identity=33.5689045936396, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI40556376, Length=265, Percent_Identity=28.3018867924528, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1786719, Length=281, Percent_Identity=36.2989323843416, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI145693186, Length=271, Percent_Identity=35.0553505535055, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1790315, Length=268, Percent_Identity=30.9701492537313, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789092, Length=284, Percent_Identity=25.7042253521127, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=277, Percent_Identity=33.5740072202166, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24655230, Length=282, Percent_Identity=35.8156028368794, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19922568, Length=282, Percent_Identity=35.8156028368794, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=23.9285714285714, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31322; Mature: 31191

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGATYAENPQKAAAQAEF
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHCCEEE
MITIVGYPKDVREVYFGAEGIFKNDCSGKVLIDMTTTEPTLAQEIYQEAKKVGAFALDAP
EEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEECCC
VSGGDLGAKNATLTIMVGGDSETYEKALPLFEKMGKTITHQGAVGSGQHTKMANQICIAG
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHCCEEEEH
TMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHCCHHHHHHCCCCCHHHHHHHHH
LGIALSEAEKMNLKLPATKGAKELYEQLADKGFENDGTQALIKLWWPNGHRPE
HHHHHHHHHHCEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
SKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGATYAENPQKAAAQAEF
CCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHCCEEE
MITIVGYPKDVREVYFGAEGIFKNDCSGKVLIDMTTTEPTLAQEIYQEAKKVGAFALDAP
EEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEECCC
VSGGDLGAKNATLTIMVGGDSETYEKALPLFEKMGKTITHQGAVGSGQHTKMANQICIAG
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHCCEEEEH
TMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHCCHHHHHHCCCCCHHHHHHHHH
LGIALSEAEKMNLKLPATKGAKELYEQLADKGFENDGTQALIKLWWPNGHRPE
HHHHHHHHHHCEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]