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The map label for this gene is ywfF

Identifier: 15674161

GI number: 15674161

Start: 2254433

End: 2255974

Strand: Reverse

Name: ywfF

Synonym: L37880

Alternate gene names: 15674161

Gene position: 2255974-2254433 (Counterclockwise)

Preceding gene: 15674162

Following gene: 15674160

Centisome position: 95.37

GC content: 36.71

Gene sequence:

>1542_bases
ATGATAAATTTAAAAATAGGACAGAAACTTCAACTCGAAATCGAGCGAATGGGAATAAATGGGGAAGGAATTGGCGTCAT
TTCAGGACGACTTGTTTTTATTCCTTATGCACTTCCAGGTGAAGAAGTTTTAGTTGAAATTACTGAAAATGCTAGAAATT
TTAGTCGTGCAAAATTGGTTAAGATTATTGAAAAATCACCAAACCGTGTGAAACCACAGGATAGAGCTTATCATGAAATG
AGTCAATCTCACATCATGCATCTTTCTTATCCAATGCAATTGGAATTTAAGCGTGATGTTATGCGTCAGGCCTTAGAAAA
ATATAAACCAGCAGGTTGGCGTAATTATGAATTGCGTGCAACTTTGGGAATGGAAAATACACTTGGTTATCGTAACAAAT
TGCAGTTCCAAGTGCGTCGTTTGAATGACGGAACAGTGATTGCGGGACTTTATCAAGAAGGAACACATCATTTGGTTAAC
CTAGATAACTGTTTGGTTCAAGATCCCAAAACCCAAGAAATTATCAATCACATTTGCCGTTTGATTGAAAAATTTGATTT
ACCTGTATATGATGAACGTAAAATCGGCGGAATTCGTACGGTCATGGTTCGTCGTTCACAAAAAACAGGTGAAGTACAAA
TTATTTTTGTGACTTCAACACCAATTATTTTGGACGGACAAGTTTGGCCTGAATTACGTGAAGAACCTTCAAAACGTCAA
AAAAATTCTTTTGTTAAATTTGATAAAATGTTGTCAGCACTGACAGAAGAATTTGAAGAAATTGTGACGGTTGCGGTCAA
TTTCCACCCAAGAAAAACCAGTGAAATCTATGGCGAACGCACACAAATTTTGTTCAATGAAAAAGAAACAATTACCGAAG
GCGTTTTGGATTATGAATTTGAATTAAGTCCTCGTGCTTTCTACCAATTGAACTCTGAGCAAGCCAATGTACTTTATGGT
GAAGCAGTTAAGGCTTTGAATCCTAAAAAAGATGACCGTGTCATTGATGCATATTGTGGTGTAGGTACGATTGGTTTTGC
GGTGGCTAAAAAAGTAAAATCGGTTCATGGAATGGATATTACACCAGAATCAATTTTTGATGCGCGTGAAAATGCCAAAC
GTTTGGGGCTTAAAAATTGCCACTATGAAATTGGTAAAGCTGAGCGAATTATTCCAAATTGGAACAAATCTGGTCATAGA
GCAACTGCAATGATTGTTGACCCACCACGTACGGGGCTTGATGATGCATTGCTTGAAACAATTACTAAATTCCCACCGGA
AAAAATGGTTTATGTCAGCTGTAACGTTTCAACTTTGGCAAAAGATTTAGTCGTTTTGGCCAATTTTTACAAAGTAGAAT
ACATCCAATCAGTGGATATGTTCCCACATACAGCAAGAACTGAAGCAGTTGTTAAATTGACAAAACGTGATGAACCTTTG
AAAATTGTTAAATTTGAACATTTGGAAGAAGAACGTAAAGCTGAAGCTTTTGCTAAAGGTAAAAAACGTGGACGCCGTCC
GCAAAAATATGGTAAATATTGA

Upstream 100 bases:

>100_bases
TGTTATAATAGTGTATCACACAATCCTTTATTAGTGATTTTTTTATAATGGATTGAAAATGGTCATTTAATTGACGAACT
AGAGGGAAAATAATTAACAA

Downstream 100 bases:

>100_bases
GAAATTTTGCTGACAGAATTATTAGTTGCTAGCAGCTAAAGTTTAAGTGAAAATTTACTGACAGAATGGATATTTTAGTT
GTTCATTCTTTTAATTACGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLVKIIEKSPNRVKPQDRAYHEM
SQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRATLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVN
LDNCLVQDPKTQEIINHICRLIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEPSKRQ
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPRKTSEIYGERTQILFNEKETITEGVLDYEFELSPRAFYQLNSEQANVLYG
EAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDITPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHR
ATAMIVDPPRTGLDDALLETITKFPPEKMVYVSCNVSTLAKDLVVLANFYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY

Sequences:

>Translated_513_residues
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLVKIIEKSPNRVKPQDRAYHEM
SQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRATLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVN
LDNCLVQDPKTQEIINHICRLIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEPSKRQ
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPRKTSEIYGERTQILFNEKETITEGVLDYEFELSPRAFYQLNSEQANVLYG
EAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDITPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHR
ATAMIVDPPRTGLDDALLETITKFPPEKMVYVSCNVSTLAKDLVVLANFYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY
>Mature_513_residues
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLVKIIEKSPNRVKPQDRAYHEM
SQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRATLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVN
LDNCLVQDPKTQEIINHICRLIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEPSKRQ
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPRKTSEIYGERTQILFNEKETITEGVLDYEFELSPRAFYQLNSEQANVLYG
EAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDITPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHR
ATAMIVDPPRTGLDDALLETITKFPPEKMVYVSCNVSTLAKDLVVLANFYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=368, Percent_Identity=27.445652173913, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI51173878, Length=368, Percent_Identity=27.445652173913, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI269784680, Length=528, Percent_Identity=25, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI269784676, Length=528, Percent_Identity=25, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI20149688, Length=528, Percent_Identity=25, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI269784678, Length=374, Percent_Identity=25.668449197861, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1787083, Length=349, Percent_Identity=25.7879656160458, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1789148, Length=476, Percent_Identity=23.5294117647059, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1790403, Length=155, Percent_Identity=28.3870967741935, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=26.0606060606061, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322909, Length=145, Percent_Identity=31.7241379310345, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24666579, Length=446, Percent_Identity=29.1479820627803, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWFF_LACLA (Q9CDP0)

Other databases:

- EMBL:   AE005176
- PIR:   C86897
- RefSeq:   NP_268336.1
- ProteinModelPortal:   Q9CDP0
- SMR:   Q9CDP0
- GeneID:   1115856
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L37880
- NMPDR:   fig|272623.1.peg.2234
- HOGENOM:   HBG690163
- OMA:   FDARENA
- ProtClustDB:   CLSK698154
- BioCyc:   LLAC272623:L37880-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: 2.1.1.- [C]

Molecular weight: Translated: 58920; Mature: 58920

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 434-434 BINDING 309-309 BINDING 338-338 BINDING 359-359 BINDING 407-407

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLV
CEECCCCCEEEEEEEEECCCCCCEEEEECEEEEEEECCCCCCEEEEEHHCCCCHHHHHHH
KIIEKSPNRVKPQDRAYHEMSQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRA
HHHHCCCCCCCCHHHHHHHHHHHCEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
TLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVNLDNCLVQDPKTQEIINHICR
EECCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCEEEECCHHHCCCCCHHHHHHHHHH
LIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEPSKRQ
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEECCCCCHHHHCCHHHHH
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPRKTSEIYGERTQILFNEKETITEGVLDYEF
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHCCCEEEEECCHHHHHHCCCCEEE
ELSPRAFYQLNSEQANVLYGEAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDI
ECCCCEEEEECCCCCCEEHHHHHHHCCCCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCC
TPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHRATAMIVDPPRTGLDDALLET
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCHHHHHHH
ITKFPPEKMVYVSCNVSTLAKDLVVLANFYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
HHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCE
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY
EEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLV
CEECCCCCEEEEEEEEECCCCCCEEEEECEEEEEEECCCCCCEEEEEHHCCCCHHHHHHH
KIIEKSPNRVKPQDRAYHEMSQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRA
HHHHCCCCCCCCHHHHHHHHHHHCEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
TLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVNLDNCLVQDPKTQEIINHICR
EECCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCEEEECCHHHCCCCCHHHHHHHHHH
LIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEPSKRQ
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEECCCCCHHHHCCHHHHH
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPRKTSEIYGERTQILFNEKETITEGVLDYEF
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHCCCEEEEECCHHHHHHCCCCEEE
ELSPRAFYQLNSEQANVLYGEAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDI
ECCCCEEEEECCCCCCEEHHHHHHHCCCCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCC
TPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHRATAMIVDPPRTGLDDALLET
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCHHHHHHH
ITKFPPEKMVYVSCNVSTLAKDLVVLANFYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
HHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCE
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY
EEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471