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The map label for this gene is polA

Identifier: 15674124

GI number: 15674124

Start: 2210775

End: 2213408

Strand: Reverse

Name: polA

Synonym: L0270

Alternate gene names: 15674124

Gene position: 2213408-2210775 (Counterclockwise)

Preceding gene: 15674127

Following gene: 15674123

Centisome position: 93.57

GC content: 35.46

Gene sequence:

>2634_bases
ATGGAGAACAAGGACAGACTTTTACTAATTGATGGGTCATCGGTTGCCTTTCGGGCCTTTTTTGCGCTATATAATCAGAT
AGATCGCTTTAAAGCGCCAAATGGGTTACATACTAATGCTATTTTTGCATTTCATACAATGCTTTCTTCATTGATGGAAC
GGATTGAGCCCACACATGTTTTGATTGCATTTGATGCAGGAAAAACAACTTTTCGTACGGAAATGTTTGCTGACTATAAA
GGTGGACGTTCAAAAACACCAGATGAATTTCGTGAGCAATTACCTTTTATCAAGGAAATGATTGAGAAATTGGGAATTCG
TCATTATGAGTTAGCTAACTATGAGGCTGATGATATTATTGGGACTTTGGACAAAATGGCGGAAGCCCCAAATGTTAATT
TTGATGTTACTATTGTGACGGGTGATAAGGATATGATTCAGCTTGTGGATGGAAATACGCGAGTTGAAATTTCTAAGAAG
GGTGTTGCTGAGTTTGAGGAGTTTACACCTGATTATCTTTTAGAAAAAATGGGATTGACTCCAGCCCAATTTATTGATTT
AAAAGCATTAATGGGCGATTCTTCTGATAATTACCCTGGCGTGACAAAAGTTGGCGAAAAAACTGGCCTTAAACTTTTAC
AAGAGTTTGGGTCGCTTGAAAATCTTTATGAAAATGTTGATTCTTTGAAAGCCAGCAAAATGAAAGAAAATCTAATTGCT
GACAAAGAAATGGCTTTTTTGTCACAACAATTGGCAACGATAAATACGAAAGCTCCAATTGAAATTGGCTTAGATGATAC
GCTTTTAAAAGGTAAAAAGGTTGATGAACTTAGTCAATTTTATGATGAAATGGGATTTGCTCAATTCAAAAGTAAATTAT
TGGCTGAGGCTGGTGGAGAAGTTACTGACGAAAAAGTTGTTGACGAAATTGATTTTGAAATAGTTACTGATGGCTCTATC
AGTGAAAAGGTAAATGCTGATGATTTCTTCTATTTGGAAACTTTAGGGGAAAATTATCATCGTGAGCAAATTGTGGCTTT
TGCCTGGGGAAATGCTGAGAAAATTTATGTTTCTAAAAATATAGATTTACTGACAAAAATGAAGTTCCCTGAAAATACTT
ATGATTTCAAGAAAAATCGGGTACTTTTGAGTCACTTGGATATCGAATTACCTTTGGTCAAATTTGATGCAATGCTTGCA
AAATATCTAATTTCAACAACAGAAGATAATAAAATTTCGACGATTGCAAGACTTTTCAATAGCGGACATTTGGCAACGGA
TGAAGAAATTTTTGGCAAAGGGACAAAAATTGCTTTACCTGATGATGCGGTTTTATTTGAACATTTAGCTCGTAAAATTA
AAGTGCTTGCCTTGGCCAAAGAAAAAATGATGGCTGAATTGCTTGAAAATGAACAAGAGCATCTTTTGTCTGACATGGAA
TTACCATTAGCAGAAGTTTTAGCAAAAATGGAAATTACAGGGATTGCTGTCAGCCAAAATACACTGGAAGAAATTGGTGC
GGAAAATGAAGAAAAATTGGCTTCTTTGACGCGTGAAATTTACGACTTGGCTGGTGAAGAATTTAATATTAATTCACCAA
AACAGTTGGGTGTGATTTTATTTGAAAAATTACAGCTACCGGTTGGAAAGAAAACAAAAACAGGCTATTCAACGGCAGTG
GATGTTTTAGAAGACTTAGCTGCTTTATCTCCGGTTGTAGCAAAAATTTTGGAATATCGTCAAATCAATAAAGTACAATC
AACTTATGTGAAAGGTCTGATTCCTCAAATTGCTGATGATGGAAAAATTCATACACGTTATGTTCAAGATTTGACTCAAA
CTGGACGTTTGAGCTCAGTTGACCCGAATTTACAAAATATTCCTGTTCGACTTGAGGAGGGACGAAAAATTCGTAAAGCA
TTTGTCCCAAGCAAAGATTCGCTTTTATTGAGTTCTGACTATTCACAAATTGAATTGCGGGTTTTGGCACATATTTCTGG
GGATGAACATTTGATTGATGCCTTTAAACATGGAGCTGATATCCATACTTCAACGGCTATGCGTGTTTTCGGCATTGAAA
AAGCAGAAGATGTGACTGCTAATGATCGTAGAAATGCTAAAGCAGTCAATTTTGGAGTAGTCTATGGAATTTCCGATTTT
GGTTTAGCAAGAAATCTTGGAATTACAAGAAAAGATGCTAAAAATTACATTGAAACTTATTTTGAACGTTATCCTGGGAT
TAAAACCTATATGGAAAATATTGTTCGCGAGGCACGAGACAAAGGTTTTGTTGAAACAATGAGTCACCGTCGTCGTAAGA
TTCCAGATATTAATGCTCGTAATTTTAATGTTCGTGGTTTTGCAGAACGGACGGCTATTAATTCACCAATTCAAGGTTCG
GCAGCTGATATCTTAAAAATTGCGATGATTAATTTGGATAAAGCTTTGAGTGCTCGTGATTTTAAATCAAAATTATTATT
GCAAGTACACGATGAAATTATCCTTGATGTTCCTTTAGAGGAATTAGATGAAATTAAGGTCTTGGTAAAACAAACGATGG
AAGAAGCGATTGAACTTGCTGTCCCATTAAAAGTGGATGAAAATACTGGTAAGACTTGGTATGAAGCTAAATAA

Upstream 100 bases:

>100_bases
ATGTTATAATTTAGTAAAGCTCGTTTTTATGTGTTTTAAGATAATATTTACTTAGATGAATGGATAAAAGTATAGAAATA
TGGAAGAGATAGGAAATAAA

Downstream 100 bases:

>100_bases
TAAAGATTATTGATAGAATTAAACTGCTCACTTTTTCACGTTTATTGACAAAATAAATAAAAACGATAATTAGCTGGGGC
TGATTATCGTTTTTTAACGG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I

Number of amino acids: Translated: 877; Mature: 877

Protein sequence:

>877_residues
MENKDRLLLIDGSSVAFRAFFALYNQIDRFKAPNGLHTNAIFAFHTMLSSLMERIEPTHVLIAFDAGKTTFRTEMFADYK
GGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDIIGTLDKMAEAPNVNFDVTIVTGDKDMIQLVDGNTRVEISKK
GVAEFEEFTPDYLLEKMGLTPAQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVDSLKASKMKENLIA
DKEMAFLSQQLATINTKAPIEIGLDDTLLKGKKVDELSQFYDEMGFAQFKSKLLAEAGGEVTDEKVVDEIDFEIVTDGSI
SEKVNADDFFYLETLGENYHREQIVAFAWGNAEKIYVSKNIDLLTKMKFPENTYDFKKNRVLLSHLDIELPLVKFDAMLA
KYLISTTEDNKISTIARLFNSGHLATDEEIFGKGTKIALPDDAVLFEHLARKIKVLALAKEKMMAELLENEQEHLLSDME
LPLAEVLAKMEITGIAVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVILFEKLQLPVGKKTKTGYSTAV
DVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADDGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKA
FVPSKDSLLLSSDYSQIELRVLAHISGDEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGS
AADILKIAMINLDKALSARDFKSKLLLQVHDEIILDVPLEELDEIKVLVKQTMEEAIELAVPLKVDENTGKTWYEAK

Sequences:

>Translated_877_residues
MENKDRLLLIDGSSVAFRAFFALYNQIDRFKAPNGLHTNAIFAFHTMLSSLMERIEPTHVLIAFDAGKTTFRTEMFADYK
GGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDIIGTLDKMAEAPNVNFDVTIVTGDKDMIQLVDGNTRVEISKK
GVAEFEEFTPDYLLEKMGLTPAQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVDSLKASKMKENLIA
DKEMAFLSQQLATINTKAPIEIGLDDTLLKGKKVDELSQFYDEMGFAQFKSKLLAEAGGEVTDEKVVDEIDFEIVTDGSI
SEKVNADDFFYLETLGENYHREQIVAFAWGNAEKIYVSKNIDLLTKMKFPENTYDFKKNRVLLSHLDIELPLVKFDAMLA
KYLISTTEDNKISTIARLFNSGHLATDEEIFGKGTKIALPDDAVLFEHLARKIKVLALAKEKMMAELLENEQEHLLSDME
LPLAEVLAKMEITGIAVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVILFEKLQLPVGKKTKTGYSTAV
DVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADDGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKA
FVPSKDSLLLSSDYSQIELRVLAHISGDEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGS
AADILKIAMINLDKALSARDFKSKLLLQVHDEIILDVPLEELDEIKVLVKQTMEEAIELAVPLKVDENTGKTWYEAK
>Mature_877_residues
MENKDRLLLIDGSSVAFRAFFALYNQIDRFKAPNGLHTNAIFAFHTMLSSLMERIEPTHVLIAFDAGKTTFRTEMFADYK
GGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDIIGTLDKMAEAPNVNFDVTIVTGDKDMIQLVDGNTRVEISKK
GVAEFEEFTPDYLLEKMGLTPAQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVDSLKASKMKENLIA
DKEMAFLSQQLATINTKAPIEIGLDDTLLKGKKVDELSQFYDEMGFAQFKSKLLAEAGGEVTDEKVVDEIDFEIVTDGSI
SEKVNADDFFYLETLGENYHREQIVAFAWGNAEKIYVSKNIDLLTKMKFPENTYDFKKNRVLLSHLDIELPLVKFDAMLA
KYLISTTEDNKISTIARLFNSGHLATDEEIFGKGTKIALPDDAVLFEHLARKIKVLALAKEKMMAELLENEQEHLLSDME
LPLAEVLAKMEITGIAVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVILFEKLQLPVGKKTKTGYSTAV
DVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADDGKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKA
FVPSKDSLLLSSDYSQIELRVLAHISGDEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINARNFNVRGFAERTAINSPIQGS
AADILKIAMINLDKALSARDFKSKLLLQVHDEIILDVPLEELDEIKVLVKQTMEEAIELAVPLKVDENTGKTWYEAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain

Homologues:

Organism=Homo sapiens, GI112420958, Length=432, Percent_Identity=36.1111111111111, Blast_Score=237, Evalue=3e-62,
Organism=Homo sapiens, GI139394648, Length=539, Percent_Identity=30.0556586270872, Blast_Score=214, Evalue=4e-55,
Organism=Escherichia coli, GI1790294, Length=510, Percent_Identity=42.7450980392157, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI226510970, Length=216, Percent_Identity=31.4814814814815, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=410, Percent_Identity=28.5365853658537, Blast_Score=177, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17933644, Length=415, Percent_Identity=33.0120481927711, Blast_Score=220, Evalue=3e-57,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DPO1_LACLA (Q9CDS1)

Other databases:

- EMBL:   AE005176
- PIR:   F86892
- RefSeq:   NP_268299.1
- ProteinModelPortal:   Q9CDS1
- SMR:   Q9CDS1
- GeneID:   1115819
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0270
- NMPDR:   fig|272623.1.peg.2197
- HOGENOM:   HBG284758
- OMA:   IPIRTEE
- ProtClustDB:   PRK05755
- BioCyc:   LLAC272623:L0270-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005622
- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337
- PRINTS:   PR00868
- SMART:   SM00474
- SMART:   SM00475
- SMART:   SM00279
- SMART:   SM00482
- TIGRFAMs:   TIGR00593

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A; SSF47807 5_3_exo_C; SSF53098 RNaseH_fold

EC number: =2.7.7.7

Molecular weight: Translated: 98734; Mature: 98734

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKDRLLLIDGSSVAFRAFFALYNQIDRFKAPNGLHTNAIFAFHTMLSSLMERIEPTHV
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
LIAFDAGKTTFRTEMFADYKGGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDII
EEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
GTLDKMAEAPNVNFDVTIVTGDKDMIQLVDGNTRVEISKKGVAEFEEFTPDYLLEKMGLT
HHHHHHHCCCCCCEEEEEEECCCCEEEEECCCEEEEEECCCHHHHHHCCHHHHHHHHCCC
PAQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVDSLKASKMKENLIA
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKEMAFLSQQLATINTKAPIEIGLDDTLLKGKKVDELSQFYDEMGFAQFKSKLLAEAGGE
HHHHHHHHHHHHHCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCC
VTDEKVVDEIDFEIVTDGSISEKVNADDFFYLETLGENYHREQIVAFAWGNAEKIYVSKN
CCHHHHHHHCCEEEEECCCCCCCCCCCCEEEHHHHCCCCCCCEEEEEEECCCEEEEEECC
IDLLTKMKFPENTYDFKKNRVLLSHLDIELPLVKFDAMLAKYLISTTEDNKISTIARLFN
CCEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SGHLATDEEIFGKGTKIALPDDAVLFEHLARKIKVLALAKEKMMAELLENEQEHLLSDME
CCCCCCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
LPLAEVLAKMEITGIAVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVIL
CCHHHHHHHHHHEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FEKLQLPVGKKTKTGYSTAVDVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKAFVPSKDSLLLSSDYSQIELR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEECCCCCEEEE
VLAHISGDEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
EEEECCCCHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEEEEEEECCCHH
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINAR
CHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
NFNVRGFAERTAINSPIQGSAADILKIAMINLDKALSARDFKSKLLLQVHDEIILDVPLE
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHH
ELDEIKVLVKQTMEEAIELAVPLKVDENTGKTWYEAK
HHHHHHHHHHHHHHHHHHEECCEEEECCCCCEEECCC
>Mature Secondary Structure
MENKDRLLLIDGSSVAFRAFFALYNQIDRFKAPNGLHTNAIFAFHTMLSSLMERIEPTHV
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
LIAFDAGKTTFRTEMFADYKGGRSKTPDEFREQLPFIKEMIEKLGIRHYELANYEADDII
EEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
GTLDKMAEAPNVNFDVTIVTGDKDMIQLVDGNTRVEISKKGVAEFEEFTPDYLLEKMGLT
HHHHHHHCCCCCCEEEEEEECCCCEEEEECCCEEEEEECCCHHHHHHCCHHHHHHHHCCC
PAQFIDLKALMGDSSDNYPGVTKVGEKTGLKLLQEFGSLENLYENVDSLKASKMKENLIA
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKEMAFLSQQLATINTKAPIEIGLDDTLLKGKKVDELSQFYDEMGFAQFKSKLLAEAGGE
HHHHHHHHHHHHHCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCC
VTDEKVVDEIDFEIVTDGSISEKVNADDFFYLETLGENYHREQIVAFAWGNAEKIYVSKN
CCHHHHHHHCCEEEEECCCCCCCCCCCCEEEHHHHCCCCCCCEEEEEEECCCEEEEEECC
IDLLTKMKFPENTYDFKKNRVLLSHLDIELPLVKFDAMLAKYLISTTEDNKISTIARLFN
CCEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SGHLATDEEIFGKGTKIALPDDAVLFEHLARKIKVLALAKEKMMAELLENEQEHLLSDME
CCCCCCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
LPLAEVLAKMEITGIAVSQNTLEEIGAENEEKLASLTREIYDLAGEEFNINSPKQLGVIL
CCHHHHHHHHHHEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FEKLQLPVGKKTKTGYSTAVDVLEDLAALSPVVAKILEYRQINKVQSTYVKGLIPQIADD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKIHTRYVQDLTQTGRLSSVDPNLQNIPVRLEEGRKIRKAFVPSKDSLLLSSDYSQIELR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEECCCCCEEEE
VLAHISGDEHLIDAFKHGADIHTSTAMRVFGIEKAEDVTANDRRNAKAVNFGVVYGISDF
EEEECCCCHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEEEEEEECCCHH
GLARNLGITRKDAKNYIETYFERYPGIKTYMENIVREARDKGFVETMSHRRRKIPDINAR
CHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
NFNVRGFAERTAINSPIQGSAADILKIAMINLDKALSARDFKSKLLLQVHDEIILDVPLE
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHH
ELDEIKVLVKQTMEEAIELAVPLKVDENTGKTWYEAK
HHHHHHHHHHHHHHHHHHEECCEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471