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The map label for this gene is rpoA

Identifier: 15674050

GI number: 15674050

Start: 2152087

End: 2153025

Strand: Reverse

Name: rpoA

Synonym: L0136

Alternate gene names: 15674050

Gene position: 2153025-2152087 (Counterclockwise)

Preceding gene: 15674051

Following gene: 15674049

Centisome position: 91.01

GC content: 38.23

Gene sequence:

>939_bases
ATGATTGAGTTTGAAAAACCAAAAATTACTAAATTTGACGAATCAGAAAATTACGGTAAATTCGTTGTTGAACCACTTGA
ACGTGGTTATGGTACAACTTTAGGTAACTCTCTGCGTCGCGTCCTTTTGTCATCACTTCCTGGTGCTGCTGTGACTTCAA
TTCAAATTGAAGGTGTTCAACACGAATTCGCAACAATCCCTGGCGTACGTGAAGATGTCATCCAAATCGTTCTTGCGGTT
AAAGGAATTGCCATCAAATCATACGTAGAGTCTGAAAAACAAATCGAACTTGACGTAACTGGTCCTATGGATGTTACTGC
TGGTGATATCTTAACTGATAGCGACATTGAAATTGTCAACAAAGACCATTACTTGTTCTCTATTGCTGAAGGTCACTCAA
TGCGTGCTGTAATGACTGTTAAAAAAGGATACGGCTATGTTCCGGATGACGAAAATAAAGTCGATGGAGCGCCAATTGGC
ACAATTGCTGTAGACTCAATTTACACACCAGTAAGTAAAGTAAATTATCAAGTAGAACCTGCCCGTGTTGGTGGAGATTC
TAGCTATGATAAATTAACACTTGAAATTACTACAAACGGTACTATCGTTTCTGACGAAGCTTTGTCACTCTCAGCTAAAA
TCTTGACAGACCACTTGAACTTGTTCGTTGATCTTTCAGAAGTTGCAGCTGAAGCCGAAACACTTGTTGTTAAAGATGAA
GTTAAAACAGAACGTGTGCTCGATAAAATTATTGAAGAAATGGACTTCTCAGTTCGTGCTTACAATGGTTTGAAACGCGC
AGGTATTAACACTGTTGCTGATATTGTTGAAATGAGCGAAGCTGACATGATTAAAGTCAAAAACCTCGGTCACAAATCAG
TAGAAGAAGTCAAAGTTAAATTGACTGAATTGGGACTTTCCCTTAAAAAAAGAAAATAA

Upstream 100 bases:

>100_bases
TGACTCCTGTACCTCACAATGGTGCACGTCCTCCAAAACGTCGTCGTGTATAATTAGTTGCTCTTGTCACTAATCACTTT
TCGCTTAGAGGAGAACATAC

Downstream 100 bases:

>100_bases
TAATATAGGAGGAAACAAATGAGCAATCGTAAACTCGGACGTACATCTTCACAACGTAAAGCTATGCTTCGTGATTTGAC
TACAGATTTGATCATCAACG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIEFEKPKITKFDESENYGKFVVEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIQIEGVQHEFATIPGVREDVIQIVLAV
KGIAIKSYVESEKQIELDVTGPMDVTAGDILTDSDIEIVNKDHYLFSIAEGHSMRAVMTVKKGYGYVPDDENKVDGAPIG
TIAVDSIYTPVSKVNYQVEPARVGGDSSYDKLTLEITTNGTIVSDEALSLSAKILTDHLNLFVDLSEVAAEAETLVVKDE
VKTERVLDKIIEEMDFSVRAYNGLKRAGINTVADIVEMSEADMIKVKNLGHKSVEEVKVKLTELGLSLKKRK

Sequences:

>Translated_312_residues
MIEFEKPKITKFDESENYGKFVVEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIQIEGVQHEFATIPGVREDVIQIVLAV
KGIAIKSYVESEKQIELDVTGPMDVTAGDILTDSDIEIVNKDHYLFSIAEGHSMRAVMTVKKGYGYVPDDENKVDGAPIG
TIAVDSIYTPVSKVNYQVEPARVGGDSSYDKLTLEITTNGTIVSDEALSLSAKILTDHLNLFVDLSEVAAEAETLVVKDE
VKTERVLDKIIEEMDFSVRAYNGLKRAGINTVADIVEMSEADMIKVKNLGHKSVEEVKVKLTELGLSLKKRK
>Mature_312_residues
MIEFEKPKITKFDESENYGKFVVEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIQIEGVQHEFATIPGVREDVIQIVLAV
KGIAIKSYVESEKQIELDVTGPMDVTAGDILTDSDIEIVNKDHYLFSIAEGHSMRAVMTVKKGYGYVPDDENKVDGAPIG
TIAVDSIYTPVSKVNYQVEPARVGGDSSYDKLTLEITTNGTIVSDEALSLSAKILTDHLNLFVDLSEVAAEAETLVVKDE
VKTERVLDKIIEEMDFSVRAYNGLKRAGINTVADIVEMSEADMIKVKNLGHKSVEEVKVKLTELGLSLKKRK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=319, Percent_Identity=40.4388714733542, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_LACLA (Q9CDY3)

Other databases:

- EMBL:   AE005176
- PIR:   D86883
- RefSeq:   NP_268225.1
- ProteinModelPortal:   Q9CDY3
- GeneID:   1115745
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0136
- NMPDR:   fig|272623.1.peg.2123
- HOGENOM:   HBG430844
- OMA:   EKPIATI
- ProtClustDB:   PRK05182
- BioCyc:   LLAC272623:L0136-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34194; Mature: 34194

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFEKPKITKFDESENYGKFVVEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIQIEGVQ
CCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCC
HEFATIPGVREDVIQIVLAVKGIAIKSYVESEKQIELDVTGPMDVTAGDILTDSDIEIVN
HHHHCCCCHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCEEEE
KDHYLFSIAEGHSMRAVMTVKKGYGYVPDDENKVDGAPIGTIAVDSIYTPVSKVNYQVEP
CCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHCCHHHCCEEECC
ARVGGDSSYDKLTLEITTNGTIVSDEALSLSAKILTDHLNLFVDLSEVAAEAETLVVKDE
EECCCCCCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHHHEEEHHHHHCCHHEEEEEHH
VKTERVLDKIIEEMDFSVRAYNGLKRAGINTVADIVEMSEADMIKVKNLGHKSVEEVKVK
HHHHHHHHHHHHHHCCCHHHHCCHHHCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
LTELGLSLKKRK
HHHHCCHHCCCC
>Mature Secondary Structure
MIEFEKPKITKFDESENYGKFVVEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIQIEGVQ
CCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCC
HEFATIPGVREDVIQIVLAVKGIAIKSYVESEKQIELDVTGPMDVTAGDILTDSDIEIVN
HHHHCCCCHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCEEEE
KDHYLFSIAEGHSMRAVMTVKKGYGYVPDDENKVDGAPIGTIAVDSIYTPVSKVNYQVEP
CCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHCCHHHCCEEECC
ARVGGDSSYDKLTLEITTNGTIVSDEALSLSAKILTDHLNLFVDLSEVAAEAETLVVKDE
EECCCCCCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHHHEEEHHHHHCCHHEEEEEHH
VKTERVLDKIIEEMDFSVRAYNGLKRAGINTVADIVEMSEADMIKVKNLGHKSVEEVKVK
HHHHHHHHHHHHHHCCCHHHHCCHHHCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
LTELGLSLKKRK
HHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471