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The map label for this gene is poxL

Identifier: 15674026

GI number: 15674026

Start: 2128720

End: 2130423

Strand: Reverse

Name: poxL

Synonym: L0199

Alternate gene names: 15674026

Gene position: 2130423-2128720 (Counterclockwise)

Preceding gene: 15674027

Following gene: 15674025

Centisome position: 90.06

GC content: 39.38

Gene sequence:

>1704_bases
ATGACAAAAATAAATGCATCAGATGCAATGTTAAAAGTTATTGAAGATTGGGGTGTCAAAAATATTTACGGTTTACCCGG
TGGGTCAATTGATTCGACAATGAACGCATTATATAACCGAAGAGACACATTAAATTACATTCAAGTTCGTCACGAAGAAG
TGGGTGCTTTAGCAGCCGTTGGGGAAGCAAAAGTAACCGGAGAAATCGGAGTAACCTTTGGTTCAGCAGGACCAGGAGCC
GTTCACTTGTTAAATGGATTATATGATGCTCAATATGACCATGTTCCAGTACTTGCCTTGGTTGGTCAAGTTCCAACGAG
TGCAATGAATACTAATTATTTCCAAGAATTAAATGAAAATCCAATGTTTGCTGACGTTAGTGTCTATAACAGAACAGTAA
TGACCGCAGAACAAATGCCAGCAGTAGTTGATGAAGCGATTCGTCAAGCCTATGCCAAACGTGGTGTTGCCGTCATTACC
ATTCCCAAAGATTTAGGTTGGCAAGAAATTGAAGATAATTATGTCTCTAGCGCTAATCTATATCAAAAACCTTTGCCACT
AGAACCTGCGGCTGAAAAAATCCAAGAAGCATGGAAAATACTAAAAGAAGCAAAACGCCCTTTGATTTATATTGGTCAAG
GAACAAGAGGAGCCCGCAAGGAAATCTTAGAACTTTCTAAAATGCTTCATGCTCCAATTTTGAGTTCTGCCTTAGGAAAA
GGAATTATTCCTGACAATGCCCCTGCTTACATGGGGTCAGCTGGTCGAGTGGCCTCTAAACCTGGGGTAGAAATTGCTTC
TAACACAGATGCTGTTTTGTTCTTAGGTTCAGACATGCCATTCCAAAGTTTCTTTATTCCTAAAGATGCTAAATACGTAC
AAGTTGATATTGACGGAACTAAATTTGGACGTCGTCATCAAGTAGATTTAGCTATTTTGGCAGATGCCAAACTGACAATT
AAGAAATTAATGGAAGTGGGAGAACAACTTCCAGAAACAAATTGGTATCGCGCAGCACTAGCAGACAAAGAAAATTGGGT
AGAGTGGCTTGAAAAACGTTCAGTCATTGACAGCTCACCTTTGGAAGTAGAACCTGTCTTTAAAGCCATTAATGACATGG
CCACTGATGACGCCATTTTCCAAGTTGATGTAGGAAATGTCACAATTGATGGAATGAGACTTCTCGATACAAGTAAGGAT
CAAAAATTCACAACAAGTGGCTGGTATGCGACAATGGGTTATGCTCTACCTGCAGCAATTGGTGCGAAAGCGCAATATCC
AGACCGTCAAGTTTGGTCAATTTCTGGTGACGGAGCTTGGGCAATGGTGATGCCTGATATTCTTACTCAGGTCAAATATA
ACTTACCAATTATCAATGTTGTTTTGACCAATAAGAGCCTTGGATTTATCGAAGCAGAACAAGATGATGCCAAACAACCA
AACTCAGGAATTGATTTGATAGGTGCTAATTTTGGCGCTTCTGCAGCGGCTTTGGGAGCTTTAGGTTTTGATGTTAATAA
TTTGTCAGAATTAAATGAAGCTTTTGCAAAAGCTAAAGAAGCGAATCGTCCAATGGTCATCAATGTCAACATCTTTGACC
ATCGTCCATTACCTGTTGAACAATTGCAACTTGATTCTGCTGAATTTGAAAAAGAATATCAAACTCAAGGATTGATTCCA
TTTAGCAAATTGATTCAAGAATAA

Upstream 100 bases:

>100_bases
TGAAAAGTAATTTATTTACTTACGGCGAAAGGAGAAATTAGTATATAAAGTAGTTTCAAAGTAGTTAGTAGTTGATAGAT
TTAAGGTCAAGGAGAATATC

Downstream 100 bases:

>100_bases
AAATAAATAATATTAGAATAAAAAGACATTAGAAGAGAGCTCTAATGTCTTTTTTAGATAGATTAAAATAAAAGAATAAA
TTAGAATAAAATTAGTTTTT

Product: pyruvate oxidase

Products: NA

Alternate protein names: Pyruvic oxidase; POX [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MTKINASDAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRRDTLNYIQVRHEEVGALAAVGEAKVTGEIGVTFGSAGPGA
VHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNENPMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVIT
IPKDLGWQEIEDNYVSSANLYQKPLPLEPAAEKIQEAWKILKEAKRPLIYIGQGTRGARKEILELSKMLHAPILSSALGK
GIIPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMPFQSFFIPKDAKYVQVDIDGTKFGRRHQVDLAILADAKLTI
KKLMEVGEQLPETNWYRAALADKENWVEWLEKRSVIDSSPLEVEPVFKAINDMATDDAIFQVDVGNVTIDGMRLLDTSKD
QKFTTSGWYATMGYALPAAIGAKAQYPDRQVWSISGDGAWAMVMPDILTQVKYNLPIINVVLTNKSLGFIEAEQDDAKQP
NSGIDLIGANFGASAAALGALGFDVNNLSELNEAFAKAKEANRPMVINVNIFDHRPLPVEQLQLDSAEFEKEYQTQGLIP
FSKLIQE

Sequences:

>Translated_567_residues
MTKINASDAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRRDTLNYIQVRHEEVGALAAVGEAKVTGEIGVTFGSAGPGA
VHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNENPMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVIT
IPKDLGWQEIEDNYVSSANLYQKPLPLEPAAEKIQEAWKILKEAKRPLIYIGQGTRGARKEILELSKMLHAPILSSALGK
GIIPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMPFQSFFIPKDAKYVQVDIDGTKFGRRHQVDLAILADAKLTI
KKLMEVGEQLPETNWYRAALADKENWVEWLEKRSVIDSSPLEVEPVFKAINDMATDDAIFQVDVGNVTIDGMRLLDTSKD
QKFTTSGWYATMGYALPAAIGAKAQYPDRQVWSISGDGAWAMVMPDILTQVKYNLPIINVVLTNKSLGFIEAEQDDAKQP
NSGIDLIGANFGASAAALGALGFDVNNLSELNEAFAKAKEANRPMVINVNIFDHRPLPVEQLQLDSAEFEKEYQTQGLIP
FSKLIQE
>Mature_566_residues
TKINASDAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRRDTLNYIQVRHEEVGALAAVGEAKVTGEIGVTFGSAGPGAV
HLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNENPMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVITI
PKDLGWQEIEDNYVSSANLYQKPLPLEPAAEKIQEAWKILKEAKRPLIYIGQGTRGARKEILELSKMLHAPILSSALGKG
IIPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMPFQSFFIPKDAKYVQVDIDGTKFGRRHQVDLAILADAKLTIK
KLMEVGEQLPETNWYRAALADKENWVEWLEKRSVIDSSPLEVEPVFKAINDMATDDAIFQVDVGNVTIDGMRLLDTSKDQ
KFTTSGWYATMGYALPAAIGAKAQYPDRQVWSISGDGAWAMVMPDILTQVKYNLPIINVVLTNKSLGFIEAEQDDAKQPN
SGIDLIGANFGASAAALGALGFDVNNLSELNEAFAKAKEANRPMVINVNIFDHRPLPVEQLQLDSAEFEKEYQTQGLIPF
SKLIQE

Specific function: Important for the aerobic growth. Decarboxylates pyruvate in four steps. The energy released is partially stored in acetyl phosphate [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=24.5080500894454, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=23.2394366197183, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=32.2404371584699, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1790104, Length=545, Percent_Identity=27.5229357798165, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=27.2727272727273, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=26.173285198556, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=22.8675136116152, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=549, Percent_Identity=25.5009107468124, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=25.4083484573503, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=585, Percent_Identity=22.5641025641026, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=551, Percent_Identity=23.7749546279492, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=560, Percent_Identity=25.5357142857143, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320123, Length=501, Percent_Identity=22.3552894211577, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=25.3996447602131, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014092
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.3.3 [H]

Molecular weight: Translated: 62089; Mature: 61958

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKINASDAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRRDTLNYIQVRHEEVGALAAV
CCCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GEAKVTGEIGVTFGSAGPGAVHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNEN
CCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCHHHHHCCCC
PMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVITIPKDLGWQEIEDNYVSSANL
CCEEEHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCHH
YQKPLPLEPAAEKIQEAWKILKEAKRPLIYIGQGTRGARKEILELSKMLHAPILSSALGK
HCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GIIPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMPFQSFFIPKDAKYVQVDIDGT
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCEEECCCCCEEEEEEECCC
KFGRRHQVDLAILADAKLTIKKLMEVGEQLPETNWYRAALADKENWVEWLEKRSVIDSSP
CCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCEEHHHCCHHHHHHHHHHHCCCCCCC
LEVEPVFKAINDMATDDAIFQVDVGNVTIDGMRLLDTSKDQKFTTSGWYATMGYALPAAI
CCHHHHHHHHHHHCCCCEEEEEECCCEEECCEEEECCCCCCCEECCCCEEHHHHHHHHHH
GAKAQYPDRQVWSISGDGAWAMVMPDILTQVKYNLPIINVVLTNKSLGFIEAEQDDAKQP
CCCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCCC
NSGIDLIGANFGASAAALGALGFDVNNLSELNEAFAKAKEANRPMVINVNIFDHRPLPVE
CCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
QLQLDSAEFEKEYQTQGLIPFSKLIQE
HHHCCHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure 
TKINASDAMLKVIEDWGVKNIYGLPGGSIDSTMNALYNRRDTLNYIQVRHEEVGALAAV
CCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GEAKVTGEIGVTFGSAGPGAVHLLNGLYDAQYDHVPVLALVGQVPTSAMNTNYFQELNEN
CCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHCCHHHHHCCCC
PMFADVSVYNRTVMTAEQMPAVVDEAIRQAYAKRGVAVITIPKDLGWQEIEDNYVSSANL
CCEEEHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCHH
YQKPLPLEPAAEKIQEAWKILKEAKRPLIYIGQGTRGARKEILELSKMLHAPILSSALGK
HCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
GIIPDNAPAYMGSAGRVASKPGVEIASNTDAVLFLGSDMPFQSFFIPKDAKYVQVDIDGT
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCEEECCCCCEEEEEEECCC
KFGRRHQVDLAILADAKLTIKKLMEVGEQLPETNWYRAALADKENWVEWLEKRSVIDSSP
CCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCEEHHHCCHHHHHHHHHHHCCCCCCC
LEVEPVFKAINDMATDDAIFQVDVGNVTIDGMRLLDTSKDQKFTTSGWYATMGYALPAAI
CCHHHHHHHHHHHCCCCEEEEEECCCEEECCEEEECCCCCCCEECCCCEEHHHHHHHHHH
GAKAQYPDRQVWSISGDGAWAMVMPDILTQVKYNLPIINVVLTNKSLGFIEAEQDDAKQP
CCCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCCC
NSGIDLIGANFGASAAALGALGFDVNNLSELNEAFAKAKEANRPMVINVNIFDHRPLPVE
CCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
QLQLDSAEFEKEYQTQGLIPFSKLIQE
HHHCCHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12566566; 8145244; 8438155 [H]