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The map label for this gene is yugD

Identifier: 15673971

GI number: 15673971

Start: 2068776

End: 2070068

Strand: Reverse

Name: yugD

Synonym: L52207

Alternate gene names: 15673971

Gene position: 2070068-2068776 (Counterclockwise)

Preceding gene: 15673972

Following gene: 15673970

Centisome position: 87.51

GC content: 39.68

Gene sequence:

>1293_bases
ATGTCAATTACAAAAACACGTCCAGAGGTGCTTGCACCAGCAGGAACACTCGAAAAACTAAAAGTCGCTATTGATTATGG
CGCAGACGCAGTATATATTGGTGGACAGGCCTATGGCTTACGTTCACGCGCAGGTAATTTTACCTTTGAAGAAATGGCTG
AAGGGGTCGCTTATGCAAGTGCCCACAACGCTAAAGTTTATGTAGCAGCCAATATGGTGACACATGAAGGGAATGAAAAA
GGGGCGGGCGAATGGTTCCGTACATTACGTGATTTAGGAATTTCAGCGGTTATCGTTTCTGACCCTGCTTTGATTGCCAT
CTGCGCTGCTGAAGCACCAGGTTTGGCCATTCACTTGTCAACCCAAGCGTCTGCTACTAACTATGAAACCTTAGAATTCT
GGAAAAATTTAGGACTTGAACGCGTAGTTTTAGCGCGTGAAGTTTCTGTAGAAGAGTTAAGAGAAATTCGTCAACATACT
GATGTTGAAATTGAAGCTTTTGTTCACGGAGCCATGTGTATTTCATACTCAGGACGTTGTGTCTTGTCAAATTATATGAG
TATGCGTGATGCTAACCGCGGTGGATGTTCACAATCTTGCCGTTGGAAATATGATTTATATGATATGCCATTTGCTGGCG
AGAGAAAATCGATTACTGGTGAAGTCACTGAAGAATTTTCAATGTCAGCCGTTGATATGTCAATGATTGAACATATTCCT
GATATGATTGAAAATGGAGTTAATTCGCTTAAAATCGAAGGAAGAATGAAATCAATTCACTATGTTTCAACAGTTTCTAA
CGTTTATAAAGCAGCTATTGATGCCTATTTGGAATCACCAGAACGTTTTGAAGAAATAAAACCAGCCTTAGTTGATGAAT
TATGGAAAGTAGCTCAACGTGAATTAGATACTGGCTTTTATTATGGAACGCCTGATGAAAATAAACAACTCTTTGGGGCT
CGTCGTAAAATTCCTGAGTACAAATTCGTCGGAGAAGTTGTTGACTTTGATGCTGAAACAATGGTAGCTACAATTCGTCA
AAGAAATGTTATTACAGAAGGTGACCAAATTGAATTTTATGGTCCAGGATTCCGCCACGTGGAAACAATGGTCAAAAATC
TTCGTTTGGCAGAAACAGATGAACATATTGACCGTGCACCAAACCCAATGACCCTTTTACATATTGATGTTCCTTTTGCG
GTTAAAGCAGGGGATATGATTCGTAAAAATGGCTCAGGTTTGATTAATCTTTATGATAAAAAAACAGGTCTTGCTAAAAC
AGTACGAGCTTAA

Upstream 100 bases:

>100_bases
GTTTTGGCTCGCAAATTTACTGACAATAAATTTGACTAAGATTTTTCTTTTTGGAGCCTTTTCAAAATACTTACAATCTT
GAAAATTGGAGAATTGAAAC

Downstream 100 bases:

>100_bases
TAAAAATAAAAAGAGAATTTTTTCTGAAATTCTCTTTTATGTCATTTTAGGACTGATAATTTCAGAATTGTCAAACTTCT
ATTTTTTATAAAATTAAGGC

Product: protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSITKTRPEVLAPAGTLEKLKVAIDYGADAVYIGGQAYGLRSRAGNFTFEEMAEGVAYASAHNAKVYVAANMVTHEGNEK
GAGEWFRTLRDLGISAVIVSDPALIAICAAEAPGLAIHLSTQASATNYETLEFWKNLGLERVVLAREVSVEELREIRQHT
DVEIEAFVHGAMCISYSGRCVLSNYMSMRDANRGGCSQSCRWKYDLYDMPFAGERKSITGEVTEEFSMSAVDMSMIEHIP
DMIENGVNSLKIEGRMKSIHYVSTVSNVYKAAIDAYLESPERFEEIKPALVDELWKVAQRELDTGFYYGTPDENKQLFGA
RRKIPEYKFVGEVVDFDAETMVATIRQRNVITEGDQIEFYGPGFRHVETMVKNLRLAETDEHIDRAPNPMTLLHIDVPFA
VKAGDMIRKNGSGLINLYDKKTGLAKTVRA

Sequences:

>Translated_430_residues
MSITKTRPEVLAPAGTLEKLKVAIDYGADAVYIGGQAYGLRSRAGNFTFEEMAEGVAYASAHNAKVYVAANMVTHEGNEK
GAGEWFRTLRDLGISAVIVSDPALIAICAAEAPGLAIHLSTQASATNYETLEFWKNLGLERVVLAREVSVEELREIRQHT
DVEIEAFVHGAMCISYSGRCVLSNYMSMRDANRGGCSQSCRWKYDLYDMPFAGERKSITGEVTEEFSMSAVDMSMIEHIP
DMIENGVNSLKIEGRMKSIHYVSTVSNVYKAAIDAYLESPERFEEIKPALVDELWKVAQRELDTGFYYGTPDENKQLFGA
RRKIPEYKFVGEVVDFDAETMVATIRQRNVITEGDQIEFYGPGFRHVETMVKNLRLAETDEHIDRAPNPMTLLHIDVPFA
VKAGDMIRKNGSGLINLYDKKTGLAKTVRA
>Mature_429_residues
SITKTRPEVLAPAGTLEKLKVAIDYGADAVYIGGQAYGLRSRAGNFTFEEMAEGVAYASAHNAKVYVAANMVTHEGNEKG
AGEWFRTLRDLGISAVIVSDPALIAICAAEAPGLAIHLSTQASATNYETLEFWKNLGLERVVLAREVSVEELREIRQHTD
VEIEAFVHGAMCISYSGRCVLSNYMSMRDANRGGCSQSCRWKYDLYDMPFAGERKSITGEVTEEFSMSAVDMSMIEHIPD
MIENGVNSLKIEGRMKSIHYVSTVSNVYKAAIDAYLESPERFEEIKPALVDELWKVAQRELDTGFYYGTPDENKQLFGAR
RKIPEYKFVGEVVDFDAETMVATIRQRNVITEGDQIEFYGPGFRHVETMVKNLRLAETDEHIDRAPNPMTLLHIDVPFAV
KAGDMIRKNGSGLINLYDKKTGLAKTVRA

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1788397, Length=397, Percent_Identity=34.5088161209068, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87081910, Length=280, Percent_Identity=36.4285714285714, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789548, Length=305, Percent_Identity=33.7704918032787, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 47872; Mature: 47741

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00099 THIOLASE_3 ; PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITKTRPEVLAPAGTLEKLKVAIDYGADAVYIGGQAYGLRSRAGNFTFEEMAEGVAYAS
CCCCCCCCCCCCCCCCHHHEEEEEECCCCEEEECCEEEECCCCCCCCCHHHHHHHHEEEC
AHNAKVYVAANMVTHEGNEKGAGEWFRTLRDLGISAVIVSDPALIAICAAEAPGLAIHLS
CCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEE
TQASATNYETLEFWKNLGLERVVLAREVSVEELREIRQHTDVEIEAFVHGAMCISYSGRC
CCCCCCCHHHHHHHHHCCHHEEHHHHHCCHHHHHHHHHHCCCEEEEEEEEEEEEECCCCC
VLSNYMSMRDANRGGCSQSCRWKYDLYDMPFAGERKSITGEVTEEFSMSAVDMSMIEHIP
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DMIENGVNSLKIEGRMKSIHYVSTVSNVYKAAIDAYLESPERFEEIKPALVDELWKVAQR
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELDTGFYYGTPDENKQLFGARRKIPEYKFVGEVVDFDAETMVATIRQRNVITEGDQIEFY
HHCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEE
GPGFRHVETMVKNLRLAETDEHIDRAPNPMTLLHIDVPFAVKAGDMIRKNGSGLINLYDK
CCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCEECCCHHHHCCCCCEEEEEEC
KTGLAKTVRA
CCCCHHHHCC
>Mature Secondary Structure 
SITKTRPEVLAPAGTLEKLKVAIDYGADAVYIGGQAYGLRSRAGNFTFEEMAEGVAYAS
CCCCCCCCCCCCCCCHHHEEEEEECCCCEEEECCEEEECCCCCCCCCHHHHHHHHEEEC
AHNAKVYVAANMVTHEGNEKGAGEWFRTLRDLGISAVIVSDPALIAICAAEAPGLAIHLS
CCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEE
TQASATNYETLEFWKNLGLERVVLAREVSVEELREIRQHTDVEIEAFVHGAMCISYSGRC
CCCCCCCHHHHHHHHHCCHHEEHHHHHCCHHHHHHHHHHCCCEEEEEEEEEEEEECCCCC
VLSNYMSMRDANRGGCSQSCRWKYDLYDMPFAGERKSITGEVTEEFSMSAVDMSMIEHIP
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DMIENGVNSLKIEGRMKSIHYVSTVSNVYKAAIDAYLESPERFEEIKPALVDELWKVAQR
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELDTGFYYGTPDENKQLFGARRKIPEYKFVGEVVDFDAETMVATIRQRNVITEGDQIEFY
HHCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEE
GPGFRHVETMVKNLRLAETDEHIDRAPNPMTLLHIDVPFAVKAGDMIRKNGSGLINLYDK
CCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCEECCCHHHHCCCCCEEEEEEC
KTGLAKTVRA
CCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]