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The map label for this gene is aspS

Identifier: 15673947

GI number: 15673947

Start: 2040170

End: 2041942

Strand: Reverse

Name: aspS

Synonym: L0346

Alternate gene names: 15673947

Gene position: 2041942-2040170 (Counterclockwise)

Preceding gene: 15673948

Following gene: 15673946

Centisome position: 86.32

GC content: 37.68

Gene sequence:

>1773_bases
ATGAAACGTACAAATTACGCGGGAAATATTACAGAAGAATACTTAAATCAAACGGTTACGGTTAAAGGCTGGGTAGCCAA
ACGCCGTAATCTTGGTGGATTAATTTTCATTGATTTACGTGACCGAGAAGGGATTGTCCAAATCGTTGTCAATCCTGAAA
CAGCAGCGGCTGATGTGGCAGAAGCAGCTGATAAAGCACGCAATGAATTTGTTCTTGAAGTGACAGGTAAAGTTGTTGAG
CGCGCAAGCAAGAATGACAAAATTAAAACTGGTGGTATTGAAATTGAAGCGACTGCCATTGAAATTTTGTCTACTTCTAA
AACAACACCTTTTGAAATTAAAGATGATGTTGAAGTTCTTGATGATACTCGTTTGAAATATCGTTACCTTGATTTACGTC
GTCCAGAAATGCTTAAAAATATCACAATGCGTCATGCGACGACTCGTTCAATTCGTGAATATTTGGATGGAGCAGGATTC
ATTGATGTTGAAACACCATTTTTAAATAAATCAACACCAGAAGGAGCGCGTGATTATCTTGTTCCATCTCGTGTTAATAA
AGGTGAATTTTATGCTCTTCCACAATCACCACAATTAATGAAACAGCTTTTAATGACTGCTGGACTTGATCGTTATTATC
AAATTGTTAAATGTTTCCGTGATGAAGATTTACGTGGTGACCGTCAGCCAGAATTCACACAAGTCGATTTAGAAACATCA
TTTTTGTCAGAAGAAGAAATTCAAGATTTGACAGAAGAGTTAATTGCTAAAGTCATGAAAGATGTGAAAGGAATTGATGT
CACTTTACCTTTCCCACGTATGAAATATGATGATGCCATGAACTTTTATGGTTCTGACAAACCTGATACTCGTTTTGAAT
TGTTACTGACAGACTTGTCAGCACTTGCTAAAACAATTGATTTCAAAGTTTTCCAAGAAGCAGAAGTTGTTAAAGCGATT
GTTGTCAAAGATGCTGCTGACAAATATTCTCGTAAGTCAATTGATAAATTGACAGAACAAGCCAAACAAAATGGGGCCAA
AGGCCTTGCATGGGTTAAATTTGAAAAAGGTGAATTTGCTGGTGGCGTTTCTAAATTCTTAGCAGAATCTACTGACAGCT
TTGTTAATGAATTGAAATTGACTGACAATGACTTGGTACTCTTCGTTGCTGACAGTCTTGATGTAGCAAATTCAGCTCTT
GGTGCGCTTCGTTTAACGATTGGTAAACAACAAGGATTGATTGATTTCCGTCAGTTCAATTTCCTTTGGGTCATTGATTG
GCCTATGTTTGAGTGGTCAGATGAAGAAGAACGTTATATGTCTGCTCATCATCCGTTTACTTTACCAACGAAAGAAACAC
AAGCCTTTTTATCTGCTGACGGACATAGCAAAGATTCTGATTTGAAAAAAGTTCGTGCCCATGCTTATGATATTGTTTTA
AATGGTTATGAACTTGGTGGAGGGTCACTAAGAATTAATACCCGCCAACTTCAAGAAGAAATGTTGAGCGCTCTTGGCTT
TAAATTAGAAGATGCAAACGAACAATTTGGTTTCTTGCTTGAAGCACTTGATTATGGTTTCCCACCACATGGCGGTTTGG
CACTCGGTCTTGACCGTTTCGTAATGTTGCTTGCTGGAAAAGACAATATCCGTGAAGTAATTGCTTTCCCTAAAAATAAC
AAAGCCTCTGACCCAATGACACAGGCTCCATCCATAGTTGCTGAAAAACAACTGGAAGAATTGAGTATTAAACTTGCAAA
CAAAGATCAATAA

Upstream 100 bases:

>100_bases
TGTTCGTGGTGTCATACCTGAAAAACAAAACTATAGATAATAAAAAGAAAGAGGAGTAAAATTTTACTTTGTGTACTAGA
AGAGTAAAATTTACATTTAT

Downstream 100 bases:

>100_bases
ATTCTCGGCGCCCTTCTAGATAGAATTGGACTTTATCAAGTTTTAAAATCTATTGGCGCTGAAAAAATGGCAAATAAATT
TTCGGCAGCAGTTGTTTATG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MKRTNYAGNITEEYLNQTVTVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAAADVAEAADKARNEFVLEVTGKVVE
RASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVLDDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGF
IDVETPFLNKSTPEGARDYLVPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEEEIQDLTEELIAKVMKDVKGIDVTLPFPRMKYDDAMNFYGSDKPDTRFELLLTDLSALAKTIDFKVFQEAEVVKAI
VVKDAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFAGGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSAL
GALRLTIGKQQGLIDFRQFNFLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHSKDSDLKKVRAHAYDIVL
NGYELGGGSLRINTRQLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRFVMLLAGKDNIREVIAFPKNN
KASDPMTQAPSIVAEKQLEELSIKLANKDQ

Sequences:

>Translated_590_residues
MKRTNYAGNITEEYLNQTVTVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAAADVAEAADKARNEFVLEVTGKVVE
RASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVLDDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGF
IDVETPFLNKSTPEGARDYLVPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEEEIQDLTEELIAKVMKDVKGIDVTLPFPRMKYDDAMNFYGSDKPDTRFELLLTDLSALAKTIDFKVFQEAEVVKAI
VVKDAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFAGGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSAL
GALRLTIGKQQGLIDFRQFNFLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHSKDSDLKKVRAHAYDIVL
NGYELGGGSLRINTRQLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRFVMLLAGKDNIREVIAFPKNN
KASDPMTQAPSIVAEKQLEELSIKLANKDQ
>Mature_590_residues
MKRTNYAGNITEEYLNQTVTVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAAADVAEAADKARNEFVLEVTGKVVE
RASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVLDDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGF
IDVETPFLNKSTPEGARDYLVPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEEEIQDLTEELIAKVMKDVKGIDVTLPFPRMKYDDAMNFYGSDKPDTRFELLLTDLSALAKTIDFKVFQEAEVVKAI
VVKDAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFAGGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSAL
GALRLTIGKQQGLIDFRQFNFLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHSKDSDLKKVRAHAYDIVL
NGYELGGGSLRINTRQLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRFVMLLAGKDNIREVIAFPKNN
KASDPMTQAPSIVAEKQLEELSIKLANKDQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=40.1666666666667, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI194272210, Length=284, Percent_Identity=26.7605633802817, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI5031815, Length=284, Percent_Identity=26.7605633802817, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI45439306, Length=272, Percent_Identity=25, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI4758762, Length=294, Percent_Identity=26.8707482993197, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788173, Length=599, Percent_Identity=48.2470784641068, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1789256, Length=293, Percent_Identity=29.0102389078498, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=26.2798634812287, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=597, Percent_Identity=35.3433835845896, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=309, Percent_Identity=24.9190938511327, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=309, Percent_Identity=24.9190938511327, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=309, Percent_Identity=24.9190938511327, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71984122, Length=304, Percent_Identity=25.6578947368421, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551876, Length=299, Percent_Identity=24.0802675585284, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=636, Percent_Identity=31.9182389937107, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=25.7839721254355, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=145, Percent_Identity=30.3448275862069, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323011, Length=267, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=37.4587458745875, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24640849, Length=262, Percent_Identity=28.2442748091603, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640851, Length=262, Percent_Identity=28.2442748091603, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=28.0334728033473, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921528, Length=280, Percent_Identity=27.8571428571429, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_LACLA (Q9CE80)

Other databases:

- EMBL:   AE005176
- PIR:   E86870
- RefSeq:   NP_268122.1
- ProteinModelPortal:   Q9CE80
- SMR:   Q9CE80
- GeneID:   1115639
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0346
- NMPDR:   fig|272623.1.peg.2018
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   LLAC272623:L0346-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66545; Mature: 66545

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTNYAGNITEEYLNQTVTVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAAADVA
CCCCCCCCCCHHHHCCCEEEEEEHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
EAADKARNEFVLEVTGKVVERASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVL
HHHHHHHCCEEEEEHHHHHHHCCCCCCEEECCEEEEEEEEEEEECCCCCCEEECCCHHHH
DDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGFIDVETPFLNKSTPEGARDYL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLSEEEIQDLTEELIAKVMKDVKGIDVTLPFPRMKYDDAMNFYGSDKPDTRFELLLTDLS
HCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCCCCCHHHHHHHHHH
ALAKTIDFKVFQEAEVVKAIVVKDAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSALGALRLTIGKQQGLIDFRQFN
CHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHEEEEECCCCCCEEEEECC
FLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHSKDSDLKKVRAHAYDIVL
EEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHEEEEE
NGYELGGGSLRINTRQLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRF
ECEEECCCEEEEEHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
VMLLAGKDNIREVIAFPKNNKASDPMTQAPSIVAEKQLEELSIKLANKDQ
HEECCCCCCHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHEEECCCCC
>Mature Secondary Structure
MKRTNYAGNITEEYLNQTVTVKGWVAKRRNLGGLIFIDLRDREGIVQIVVNPETAAADVA
CCCCCCCCCCHHHHCCCEEEEEEHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
EAADKARNEFVLEVTGKVVERASKNDKIKTGGIEIEATAIEILSTSKTTPFEIKDDVEVL
HHHHHHHCCEEEEEHHHHHHHCCCCCCEEECCEEEEEEEEEEEECCCCCCEEECCCHHHH
DDTRLKYRYLDLRRPEMLKNITMRHATTRSIREYLDGAGFIDVETPFLNKSTPEGARDYL
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVNKGEFYALPQSPQLMKQLLMTAGLDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLSEEEIQDLTEELIAKVMKDVKGIDVTLPFPRMKYDDAMNFYGSDKPDTRFELLLTDLS
HCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCCCCCHHHHHHHHHH
ALAKTIDFKVFQEAEVVKAIVVKDAADKYSRKSIDKLTEQAKQNGAKGLAWVKFEKGEFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GGVSKFLAESTDSFVNELKLTDNDLVLFVADSLDVANSALGALRLTIGKQQGLIDFRQFN
CHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHEEEEECCCCCCEEEEECC
FLWVIDWPMFEWSDEEERYMSAHHPFTLPTKETQAFLSADGHSKDSDLKKVRAHAYDIVL
EEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHEEEEE
NGYELGGGSLRINTRQLQEEMLSALGFKLEDANEQFGFLLEALDYGFPPHGGLALGLDRF
ECEEECCCEEEEEHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
VMLLAGKDNIREVIAFPKNNKASDPMTQAPSIVAEKQLEELSIKLANKDQ
HEECCCCCCHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471