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The map label for this gene is yudI

Identifier: 15673943

GI number: 15673943

Start: 2035883

End: 2036887

Strand: Direct

Name: yudI

Synonym: L19272

Alternate gene names: 15673943

Gene position: 2035883-2036887 (Clockwise)

Preceding gene: 15673942

Following gene: 15673956

Centisome position: 86.06

GC content: 40.7

Gene sequence:

>1005_bases
ATGACTATGGACAAAATTTACAACGAATCGTGGAAAATTGGCGATATCGAAATAAAAAATAAAATTGTGTTAGCACCAAT
GGCCGGAATTACGAATAAAGCTTTTCTGACTACTGCTAAAGAATTTGGAGCTGGACTTGTTGTAACTGAAATGATTTCTG
ATAAAGGAATTGAACATCGCAACAAAAAGACTTTGGAAATGATGGATTTTGAAGGGGTCCCTCATCCACTCTCAATGCAA
ATTTTTGGGGGTGAAGTAGATACTCTGGTTGAAGCAGCCAAATTTGTTGAAGCAAATACTGTTGCTGATATTATTGATAT
CAATATGGGTTGCCCAGTACCCAAAGTCACTAAAAACGAAGCCGGTTCTCGTCTTTTGCTTGATCCTGATAAAGTTTATG
ATGTGGTCTCTGCTGTTTCTAAAGCTATTTCAAGACCACTAACAGTGAAAATCCGAATTGGTTGGGATGACGACCACCTT
TTTGCAGTTGAAAATGCTAAAGCGATTGAAGCGGGAGGCGGAGCGGCTGTTTCTATGCATGCACGAACCAAAGCACAAGC
TTATACAGGAAATGCTCAAGAAAATTGGCACTGGCTCAAAAAATTGACCGAAAATGTCAATATTCCGGTCATCGGAAATG
GCGATGTCAAGACTCCTGAAGACGCGAAACGCATGATTGATGAAACAGGCGTCACTGCGGTCATGATGGGAAGAGCAGCC
CTTGGAAACCCTTGGATTCTTCATCGTACCGAGCATTATCTACGAACAGGTGAACTATTGCCTGAACCAACAGTTGCGGG
AAAAATGGAAATTGCAAAACTTCACTTGGCACGTCTCGTCGAATTAAAAGGTGATAATCTTGCTAGCCGAGAATTTCGTC
AGCACGCAGCATATTACCTAAAAGGAGCTTCTCGTGCAGCAAAAGTAAAAGTTGCTGTCAATCAAGCAGAATCACAAGCA
GAAATTATCACTATTCTCGATAATTTTGTGAATGGAATCAAATAA

Upstream 100 bases:

>100_bases
GTCAGTAAAACTTACTGACAGAATTCACCGACAGATCTGTCAGTAACTTTTTAAGCCTTAAAACCTTGTGAAATCTCAAA
AAATTGCTATAATATTCTTT

Downstream 100 bases:

>100_bases
AACGATATGAAATCTGGAAATTTCCAGGTTTTTTAGTTATAATAAATATAGAGATAAATATTCAAACAATTCCGAAAATA
GAAAGGGTTTTCTGTATGCG

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTMDKIYNESWKIGDIEIKNKIVLAPMAGITNKAFLTTAKEFGAGLVVTEMISDKGIEHRNKKTLEMMDFEGVPHPLSMQ
IFGGEVDTLVEAAKFVEANTVADIIDINMGCPVPKVTKNEAGSRLLLDPDKVYDVVSAVSKAISRPLTVKIRIGWDDDHL
FAVENAKAIEAGGGAAVSMHARTKAQAYTGNAQENWHWLKKLTENVNIPVIGNGDVKTPEDAKRMIDETGVTAVMMGRAA
LGNPWILHRTEHYLRTGELLPEPTVAGKMEIAKLHLARLVELKGDNLASREFRQHAAYYLKGASRAAKVKVAVNQAESQA
EIITILDNFVNGIK

Sequences:

>Translated_334_residues
MTMDKIYNESWKIGDIEIKNKIVLAPMAGITNKAFLTTAKEFGAGLVVTEMISDKGIEHRNKKTLEMMDFEGVPHPLSMQ
IFGGEVDTLVEAAKFVEANTVADIIDINMGCPVPKVTKNEAGSRLLLDPDKVYDVVSAVSKAISRPLTVKIRIGWDDDHL
FAVENAKAIEAGGGAAVSMHARTKAQAYTGNAQENWHWLKKLTENVNIPVIGNGDVKTPEDAKRMIDETGVTAVMMGRAA
LGNPWILHRTEHYLRTGELLPEPTVAGKMEIAKLHLARLVELKGDNLASREFRQHAAYYLKGASRAAKVKVAVNQAESQA
EIITILDNFVNGIK
>Mature_333_residues
TMDKIYNESWKIGDIEIKNKIVLAPMAGITNKAFLTTAKEFGAGLVVTEMISDKGIEHRNKKTLEMMDFEGVPHPLSMQI
FGGEVDTLVEAAKFVEANTVADIIDINMGCPVPKVTKNEAGSRLLLDPDKVYDVVSAVSKAISRPLTVKIRIGWDDDHLF
AVENAKAIEAGGGAAVSMHARTKAQAYTGNAQENWHWLKKLTENVNIPVIGNGDVKTPEDAKRMIDETGVTAVMMGRAAL
GNPWILHRTEHYLRTGELLPEPTVAGKMEIAKLHLARLVELKGDNLASREFRQHAAYYLKGASRAAKVKVAVNQAESQAE
IITILDNFVNGIK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=29.4871794871795, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=29.6137339055794, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI40807366, Length=224, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI8923374, Length=248, Percent_Identity=31.4516129032258, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=31.2775330396476, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789660, Length=319, Percent_Identity=36.3636363636364, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1788462, Length=236, Percent_Identity=30.0847457627119, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693211, Length=247, Percent_Identity=28.7449392712551, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=232, Percent_Identity=31.8965517241379, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=30.5084745762712, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=310, Percent_Identity=26.7741935483871, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=249, Percent_Identity=26.5060240963855, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=271, Percent_Identity=26.1992619926199, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=25.6637168141593, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24585320, Length=242, Percent_Identity=31.8181818181818, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24580595, Length=285, Percent_Identity=26.3157894736842, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920448, Length=285, Percent_Identity=26.3157894736842, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921524, Length=312, Percent_Identity=23.7179487179487, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45549423, Length=266, Percent_Identity=29.3233082706767, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36597; Mature: 36465

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDKIYNESWKIGDIEIKNKIVLAPMAGITNKAFLTTAKEFGAGLVVTEMISDKGIEHR
CCHHHHHCCCCCCCEEEECCEEEEEECCCCCCCHHEEEHHHHCCCEEEEHHHHHCCCCCC
NKKTLEMMDFEGVPHPLSMQIFGGEVDTLVEAAKFVEANTVADIIDINMGCPVPKVTKNE
CCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCC
AGSRLLLDPDKVYDVVSAVSKAISRPLTVKIRIGWDDDHLFAVENAKAIEAGGGAAVSMH
CCCEEEECHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCEEECCCCCEEEEE
ARTKAQAYTGNAQENWHWLKKLTENVNIPVIGNGDVKTPEDAKRMIDETGVTAVMMGRAA
CCCCHHEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEECCHH
LGNPWILHRTEHYLRTGELLPEPTVAGKMEIAKLHLARLVELKGDNLASREFRQHAAYYL
CCCCEEEECCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KGASRAAKVKVAVNQAESQAEIITILDNFVNGIK
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMDKIYNESWKIGDIEIKNKIVLAPMAGITNKAFLTTAKEFGAGLVVTEMISDKGIEHR
CHHHHHCCCCCCCEEEECCEEEEEECCCCCCCHHEEEHHHHCCCEEEEHHHHHCCCCCC
NKKTLEMMDFEGVPHPLSMQIFGGEVDTLVEAAKFVEANTVADIIDINMGCPVPKVTKNE
CCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCC
AGSRLLLDPDKVYDVVSAVSKAISRPLTVKIRIGWDDDHLFAVENAKAIEAGGGAAVSMH
CCCEEEECHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCEEECCCCCEEEEE
ARTKAQAYTGNAQENWHWLKKLTENVNIPVIGNGDVKTPEDAKRMIDETGVTAVMMGRAA
CCCCHHEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEECCHH
LGNPWILHRTEHYLRTGELLPEPTVAGKMEIAKLHLARLVELKGDNLASREFRQHAAYYL
CCCCEEEECCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KGASRAAKVKVAVNQAESQAEIITILDNFVNGIK
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]