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The map label for this gene is purA

Identifier: 15673934

GI number: 15673934

Start: 2028748

End: 2030040

Strand: Reverse

Name: purA

Synonym: L12179

Alternate gene names: 15673934

Gene position: 2030040-2028748 (Counterclockwise)

Preceding gene: 15673935

Following gene: 15673928

Centisome position: 85.82

GC content: 40.29

Gene sequence:

>1293_bases
ATGCCATCAGTAGTAGTTGTCGGAACGCAATGGGGAGACGAAGGAAAAGGTAAAATTACAGATTTCCTAAGCTCAAATGC
AGAAGTTATTGCCCGTTATCAAGGGGGAGACAATGCAGGTCATACCATCGTCATCGACGGCAAAAAGTTCAAACTTCACC
TTATTCCATCAGGAATTTTCTTCCCTGAAAAGATTTCCGTTATTGGGAATGGTGTCGTTGTTAACCCAAAATCACTGATT
GAAGAAATCGCTTATCTCGCTGAAAATGGAGTTTCTGCAGAGAGCTTACGTATTTCAGACCGTGCACATGTTATCTTGCC
TTACCATAAAAAATTAGACTTCCTTCAAGAAGAAGCCAAAGGCGATAAGAAGATTGGGACAACCATTAAAGGGATTGGCC
CAGCCTATATGGATAAAGCAGCCCGTGTCGGAATTCGTGTTGCAGACTTACTTGACAAAGAAATCTTCGAGGAACGTTTA
CGTACAAACCTTGAAGCTAAAAACCGTGAATTTGTAAAAATGTATGATTCAGAACCAATCGAATTTGAAGAAATTTTTGA
AGAATACTATGAATACGGTCAACAACTTAAAAAATATGTGACAGATACTTCAGTTATCTTGAATGATGCCCTTGATGCAG
GAAAACGTGTCCTTTTTGAAGGTGCGCAAGGGGTAATGCTTGATATTGACCAAGGAACATATCCATTTGTAACTTCATCA
AACCCAGTAGCTGGTGGGGTAACGATTGGCTCTGGTGTGGGTCCATCAAAAATTTCAAAAGTTGTTGGTGTTTGTAAAGC
CTATACTTCACGTGTGGGTGATGGTCCATTCCCAACAGAACTTTTTGATGAAGTTGGACATCAAATTCGTGAAGTAGGAC
ATGAATATGGAACAACAACAGGACGTCCACGTCGTGTTGGTTGGTTTGACTCAGTCGTAATGCGTCATGCAAAACGTGTT
TCTGGCTTGACAAATCTTAGCTTGAATTCAATTGACGTTCTCTCAGGACTTGAAACAGTAAAAATTTGTGTTGCTTACGA
ACGTAGTAATGGTGAACAAATTACTCATTATCCAGCATCACTTAAGGAATTAGCAGATTGCAAACCAATCTATGAAGAAT
TGCCAGGATGGTCTGAAGATATTACTTCATGCCGAACTTTAGAAGAGTTACCAGAAGCTGCTCGTAACTATGTTCGTCGT
GTTGGTGAACTAGTTGGCGTACGTATCTCGACTTTCTCAGTAGGTCCTGGTCGTGAACAAACTAATGTTTTAGAATCAGT
TTGGGGCGTATAA

Upstream 100 bases:

>100_bases
AGTTGAAGAAGTTTTTTAGAAATGCCAGCCAAGCGAACTTGGAGCGTGCAAAATATTTTAAGAAAAGTGGATAGTCAAAA
CTATCCAAAAGGAGAATATT

Downstream 100 bases:

>100_bases
AGTCAAAATAAAAAAGCCAGAGCATGCTCTGACTTTTTTTGTTTACAAGAAGGGTCCAAAGACTTGTGAACTAGCATTTT
TATTATTGACGTTGATATCA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGVVVNPKSLI
EEIAYLAENGVSAESLRISDRAHVILPYHKKLDFLQEEAKGDKKIGTTIKGIGPAYMDKAARVGIRVADLLDKEIFEERL
RTNLEAKNREFVKMYDSEPIEFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTTGRPRRVGWFDSVVMRHAKRV
SGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPASLKELADCKPIYEELPGWSEDITSCRTLEELPEAARNYVRR
VGELVGVRISTFSVGPGREQTNVLESVWGV

Sequences:

>Translated_430_residues
MPSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGVVVNPKSLI
EEIAYLAENGVSAESLRISDRAHVILPYHKKLDFLQEEAKGDKKIGTTIKGIGPAYMDKAARVGIRVADLLDKEIFEERL
RTNLEAKNREFVKMYDSEPIEFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTTGRPRRVGWFDSVVMRHAKRV
SGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPASLKELADCKPIYEELPGWSEDITSCRTLEELPEAARNYVRR
VGELVGVRISTFSVGPGREQTNVLESVWGV
>Mature_429_residues
PSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGVVVNPKSLIE
EIAYLAENGVSAESLRISDRAHVILPYHKKLDFLQEEAKGDKKIGTTIKGIGPAYMDKAARVGIRVADLLDKEIFEERLR
TNLEAKNREFVKMYDSEPIEFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSSN
PVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTTGRPRRVGWFDSVVMRHAKRVS
GLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPASLKELADCKPIYEELPGWSEDITSCRTLEELPEAARNYVRRV
GELVGVRISTFSVGPGREQTNVLESVWGV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=45.260663507109, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI34577063, Length=424, Percent_Identity=42.4528301886792, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI40316944, Length=393, Percent_Identity=44.0203562340967, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=49.1725768321513, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=43.4272300469484, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=43.4272300469484, Blast_Score=335, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=41.4519906323185, Blast_Score=342, Evalue=5e-95,
Organism=Drosophila melanogaster, GI21356033, Length=418, Percent_Identity=44.4976076555024, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_LACLA (Q9CE93)

Other databases:

- EMBL:   AE005176
- PIR:   H86868
- RefSeq:   NP_268109.1
- ProteinModelPortal:   Q9CE93
- SMR:   Q9CE93
- GeneID:   1115625
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L12179
- NMPDR:   fig|272623.1.peg.2004
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   LLAC272623:L12179-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47326; Mature: 47195

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIF
CCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
FPEKISVIGNGVVVNPKSLIEEIAYLAENGVSAESLRISDRAHVILPYHKKLDFLQEEAK
CCCHHHEECCCEEECHHHHHHHHHHHHHCCCCHHHEEECCCCEEEECCHHHHHHHHHHHC
GDKKIGTTIKGIGPAYMDKAARVGIRVADLLDKEIFEERLRTNLEAKNREFVKMYDSEPI
CCHHHCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
EFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVMRHAKRVSGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPAS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCEEEEEEEEECCCCCEEEECCHH
LKELADCKPIYEELPGWSEDITSCRTLEELPEAARNYVRRVGELVGVRISTFSVGPGREQ
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
TNVLESVWGV
HHHHHHHHCC
>Mature Secondary Structure 
PSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIF
CCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
FPEKISVIGNGVVVNPKSLIEEIAYLAENGVSAESLRISDRAHVILPYHKKLDFLQEEAK
CCCHHHEECCCEEECHHHHHHHHHHHHHCCCCHHHEEECCCCEEEECCHHHHHHHHHHHC
GDKKIGTTIKGIGPAYMDKAARVGIRVADLLDKEIFEERLRTNLEAKNREFVKMYDSEPI
CCHHHCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
EFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVMRHAKRVSGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPAS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCEEEEEEEEECCCCCEEEECCHH
LKELADCKPIYEELPGWSEDITSCRTLEELPEAARNYVRRVGELVGVRISTFSVGPGREQ
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
TNVLESVWGV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471