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The map label for this gene is metK

Identifier: 15673884

GI number: 15673884

Start: 1969978

End: 1971177

Strand: Reverse

Name: metK

Synonym: L153408

Alternate gene names: 15673884

Gene position: 1971177-1969978 (Counterclockwise)

Preceding gene: 15673886

Following gene: 30024061

Centisome position: 83.33

GC content: 40.17

Gene sequence:

>1200_bases
ATGTCAGAAAAACATTTATTTACATCAGAATCAGTATCAGAAGGCCATCCAGATAAGGTTGCAGACCAAATCTCAGATGC
CATTCTTGATGCCATTCTCGCACAAGACCCACATGCACACGTTGCTTGTGAAACGGTCGTATATACAGGGACGGTCAATG
TCTTTGGTGAAATCTCAACATCAGCTTATGTTGATATTGCTCATGTCGTTCGTGAAACAATCAAAAAAATTGGCTATACA
GATAGCGAAAATGGATTTGATTATAAATCAGTGGGCGTTCACGTCTCACTTGTTGAGCAATCAAGCGATATTGCACAAGG
GGTAAATGAAGCCGAAGAAGTTCGCGATAAAAATGGCCAATTAGTTGACCCGCTTGATTTGATTGGTGCTGGTGACCAAG
GAATGATGTTCGGTTTTGCGACTAACGAAACTGCCGAATATATGCCACTCGCCATCTCACTTTCACATAAATTAGTTAAA
AAATTAGCTGATTTACGTAAATCAGGTGAAATTTCATACTTGCGTCCTGATGCAAAATCACAAGTAACTGTAGAATATGA
TGATAATGGCAAAGCAAAACGCATTGACACAGTCGTTATTTCTACTCAACATGCTGCGTCAGCAAGAAACGAAGAAATTC
ACGATGATGTGATTAATAAAGTCATCAAGGCTGTTATTCCAGCTGAACTTTTAGATGATGAAACTAAATACTTCATCAAT
CCAACAGGACGTTTTGTTATTGGTGGTCCTCAAGGGGATTCAGGCTTGACTGGACGTAAAATTATTGTTGATACTTATGG
TGGGTATGCTCCCCACGGTGGTGGAGCTTTCTCTGGTAAGGATGCAACAAAAGTTGACCGTTCAGCTTCTTACGCAGCAC
GTTATGTAGCCAAAAATATTGTGGCAGCTGGACTTGCAGAAAAAGCGCAAATTCAACTTTCATACGCTATTGGTGTTGCG
ACACCAACGTCAATCAATGTTGATACTTTTGGCACAGGTAAAGTCTCAGATGATGAACTTTTAGCAGCAATTCGTAAAGT
ATTTGATCTTCGCCCAGCAGGAATTATTCAAATGCTTGACTTACTCCGTCCTATTTACGGACAAACAGCAGCTTACGGGC
ATTTCGGCCGTACAGATGTAGAATTACCATGGGAACAAACAGATAAGGTTGAAGAACTTAAATCCGTTTTAGGTAAATAA

Upstream 100 bases:

>100_bases
TGAAGCGCCAACGGCTATCCTATGAAGGTTTTTCGTCAGCAAATATTAGATAAGTTTAAAGACGCCTTGTAACCAAAATT
TAGATTGGGGGACATTTTTA

Downstream 100 bases:

>100_bases
TCGGCCTCGCCGACCTTCGTTCGGTCTACGTCGCCATTGTCTCCTCCGCTGTCCTTTCTCGCTAATGGGCTAAAGCCCAA
AGTCGAAAGGCACTTCGGCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEISTSAYVDIAHVVRETIKKIGYT
DSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQLVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVK
KLADLRKSGEISYLRPDAKSQVTVEYDDNGKAKRIDTVVISTQHAASARNEEIHDDVINKVIKAVIPAELLDDETKYFIN
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNIVAAGLAEKAQIQLSYAIGVA
TPTSINVDTFGTGKVSDDELLAAIRKVFDLRPAGIIQMLDLLRPIYGQTAAYGHFGRTDVELPWEQTDKVEELKSVLGK

Sequences:

>Translated_399_residues
MSEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEISTSAYVDIAHVVRETIKKIGYT
DSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQLVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVK
KLADLRKSGEISYLRPDAKSQVTVEYDDNGKAKRIDTVVISTQHAASARNEEIHDDVINKVIKAVIPAELLDDETKYFIN
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNIVAAGLAEKAQIQLSYAIGVA
TPTSINVDTFGTGKVSDDELLAAIRKVFDLRPAGIIQMLDLLRPIYGQTAAYGHFGRTDVELPWEQTDKVEELKSVLGK
>Mature_398_residues
SEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEISTSAYVDIAHVVRETIKKIGYTD
SENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQLVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVKK
LADLRKSGEISYLRPDAKSQVTVEYDDNGKAKRIDTVVISTQHAASARNEEIHDDVINKVIKAVIPAELLDDETKYFINP
TGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNIVAAGLAEKAQIQLSYAIGVAT
PTSINVDTFGTGKVSDDELLAAIRKVFDLRPAGIIQMLDLLRPIYGQTAAYGHFGRTDVELPWEQTDKVEELKSVLGK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=58.2474226804124, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=57.840616966581, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=62.2784810126582, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17538498, Length=399, Percent_Identity=57.8947368421053, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=407, Percent_Identity=57.985257985258, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=57.8005115089514, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=402, Percent_Identity=54.4776119402985, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565905, Length=407, Percent_Identity=52.8255528255528, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=351, Percent_Identity=54.7008547008547, Blast_Score=353, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=54.5189504373178, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=329, Percent_Identity=55.0151975683891, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=66.6666666666667, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=85, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=58.6118251928021, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=57.7608142493639, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=56.0102301790281, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=56.0102301790281, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=56.0102301790281, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=56.0102301790281, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=56.0102301790281, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=56.0102301790281, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=55.2699228791774, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=55.2699228791774, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=391, Percent_Identity=52.9411764705882, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=128, Percent_Identity=54.6875, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_LACLA (Q9CEE0)

Other databases:

- EMBL:   AE005176
- PIR:   F86862
- RefSeq:   NP_268059.1
- ProteinModelPortal:   Q9CEE0
- GeneID:   1115568
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L153408
- NMPDR:   fig|272623.1.peg.1949
- HOGENOM:   HBG443662
- OMA:   ENHEPLE
- ProtClustDB:   PRK05250
- BioCyc:   LLAC272623:L153408-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43018; Mature: 42887

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEECCC
SAYVDIAHVVRETIKKIGYTDSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCEEEEEECCCHHHHHCCHHHHHHHHCCCC
LVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVKKLADLRKSGEISYLRPDAKS
EECCHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCC
QVTVEYDDNGKAKRIDTVVISTQHAASARNEEIHDDVINKVIKAVIPAELLDDETKYFIN
EEEEEECCCCCCEEEEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHCHHHHCCCCEEEEC
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAQIQLSYAIGVATPTSINVDTFGTGKVSDDELLAAIRKVFDLRPAGIIQMLD
HHHCCCCCCEEEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LLRPIYGQTAAYGHFGRTDVELPWEQTDKVEELKSVLGK
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEIST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEECCC
SAYVDIAHVVRETIKKIGYTDSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCEEEEEECCCHHHHHCCHHHHHHHHCCCC
LVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVKKLADLRKSGEISYLRPDAKS
EECCHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCC
QVTVEYDDNGKAKRIDTVVISTQHAASARNEEIHDDVINKVIKAVIPAELLDDETKYFIN
EEEEEECCCCCCEEEEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHCHHHHCCCCEEEEC
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAQIQLSYAIGVATPTSINVDTFGTGKVSDDELLAAIRKVFDLRPAGIIQMLD
HHHCCCCCCEEEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LLRPIYGQTAAYGHFGRTDVELPWEQTDKVEELKSVLGK
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471