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The map label for this gene is yseB

Identifier: 15673764

GI number: 15673764

Start: 1841391

End: 1842632

Strand: Direct

Name: yseB

Synonym: L24742

Alternate gene names: 15673764

Gene position: 1841391-1842632 (Clockwise)

Preceding gene: 15673752

Following gene: 15673765

Centisome position: 77.84

GC content: 31.88

Gene sequence:

>1242_bases
ATGGAAAAAAATAAATTTAATTTTTCAAAACTGGCCGCACTTTTTACTGATTATCCAGCTCTTAAAATAGGCGAAATTTC
ATATAGCTACCAACAAATCAATGATTTAATACAAAGAAAATTAACAGATTATAGCCACTCCTCTAAAACTGTTATTACTC
TAATAAATGATAGTCCCTTGAACTTTATTATTGATTTTCTCGCAATTATCGAAAGTGGAAATTACCCACTTGTCGTGGAT
AAATCTTTTAAGAAAAAGTCTTTTAAATTCGGACTTATCCCTGAGGATACTCTGTTTTTAGCAAGTACATCTGGGACAAC
TGGACCGCCCAAAATCTATTATCGTAACTGGGAATCTTGGAAAATGGGTTTTGAAGTTTGTGATGATTTATTTCATTTGA
GCAACTCACAAGCTCTTGCAACTACTAGCCCACTAACTACTTCATTAGGACTTCATACACTCATGTGTGCTTTATATTTA
GGAAAAACCTTCCTAACAATTACAAATCCTTCACAATTTATAAATATTACAGAAAACTATGCTCTCTTTACCGTCCCAAC
CTATCTCTTAAATAACCTCAATGAATTATTTGCTTTAACTTCTCCAGAGATTATTTTTTTAGGTGGAGGAACACTGAGCC
CAGAGGCGATTAATAAAGTAAGAAAAAAACTTCCGCAAACTCAAATGATTGAGTTTTATGGAAGCTCTGAAACAAGTTTT
ATTTCATGGCAAGCAGTTAATGAGGGTAAAAAAACATCATCTGTAGGAAAACTCTTTCCACACGTAGAACTTACTTTAGG
ACCAAAATATCGTCTTACAGTTAAAAGTCCTTATTTATTTTCTGGCTATCTTAATCAACCTTATCCACAATCATGGACAA
CTGATGATTTAGGAACCTTAAAAAATAATCATCTTTATTTATTTGGACGTCGGTCTGACATTATCGAACATGGTGCAAAT
AAAATTTTTCCAGAAGAAATTGAGCGTATGGCAAAAGATTTATGTGAAGACTGCATCGCTTTTGGAGTTTTTGATGAAAG
ATATAGTCAAAAAATTGCTCTACTACTCTTAAAACCTATCGAGAAAGAGAAATTAATCAAAATACTAGCTCAAAGACTTC
CTAAATACAAACTTCCTCAAATATATTTAGAAACTCCCACTCTTAATTTTACTAAAAATCAAAAAATATCCAGAAAAGAA
TTAGAAAAAGAATATTTTAGAGGTGTCTATCATGAGCTTTAA

Upstream 100 bases:

>100_bases
ATTATACTGATATCATATCAAACAGTAACCCTAAAATCAAATTTAGGTTAACTGTTAATTTTATTATTTGCTTTTTTATT
CACACAAAGGAAAATTTTAG

Downstream 100 bases:

>100_bases
AAATCCCGTCGGTATCATAGCAGCAAACCGTTTACCTATAGGAAAAATCAATGGTTTTTATAAAAAAATTTCTCCTGAAA
CTTTATATCAAAATCTAATT

Product: hypothetical protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MEKNKFNFSKLAALFTDYPALKIGEISYSYQQINDLIQRKLTDYSHSSKTVITLINDSPLNFIIDFLAIIESGNYPLVVD
KSFKKKSFKFGLIPEDTLFLASTSGTTGPPKIYYRNWESWKMGFEVCDDLFHLSNSQALATTSPLTTSLGLHTLMCALYL
GKTFLTITNPSQFINITENYALFTVPTYLLNNLNELFALTSPEIIFLGGGTLSPEAINKVRKKLPQTQMIEFYGSSETSF
ISWQAVNEGKKTSSVGKLFPHVELTLGPKYRLTVKSPYLFSGYLNQPYPQSWTTDDLGTLKNNHLYLFGRRSDIIEHGAN
KIFPEEIERMAKDLCEDCIAFGVFDERYSQKIALLLLKPIEKEKLIKILAQRLPKYKLPQIYLETPTLNFTKNQKISRKE
LEKEYFRGVYHEL

Sequences:

>Translated_413_residues
MEKNKFNFSKLAALFTDYPALKIGEISYSYQQINDLIQRKLTDYSHSSKTVITLINDSPLNFIIDFLAIIESGNYPLVVD
KSFKKKSFKFGLIPEDTLFLASTSGTTGPPKIYYRNWESWKMGFEVCDDLFHLSNSQALATTSPLTTSLGLHTLMCALYL
GKTFLTITNPSQFINITENYALFTVPTYLLNNLNELFALTSPEIIFLGGGTLSPEAINKVRKKLPQTQMIEFYGSSETSF
ISWQAVNEGKKTSSVGKLFPHVELTLGPKYRLTVKSPYLFSGYLNQPYPQSWTTDDLGTLKNNHLYLFGRRSDIIEHGAN
KIFPEEIERMAKDLCEDCIAFGVFDERYSQKIALLLLKPIEKEKLIKILAQRLPKYKLPQIYLETPTLNFTKNQKISRKE
LEKEYFRGVYHEL
>Mature_413_residues
MEKNKFNFSKLAALFTDYPALKIGEISYSYQQINDLIQRKLTDYSHSSKTVITLINDSPLNFIIDFLAIIESGNYPLVVD
KSFKKKSFKFGLIPEDTLFLASTSGTTGPPKIYYRNWESWKMGFEVCDDLFHLSNSQALATTSPLTTSLGLHTLMCALYL
GKTFLTITNPSQFINITENYALFTVPTYLLNNLNELFALTSPEIIFLGGGTLSPEAINKVRKKLPQTQMIEFYGSSETSF
ISWQAVNEGKKTSSVGKLFPHVELTLGPKYRLTVKSPYLFSGYLNQPYPQSWTTDDLGTLKNNHLYLFGRRSDIIEHGAN
KIFPEEIERMAKDLCEDCIAFGVFDERYSQKIALLLLKPIEKEKLIKILAQRLPKYKLPQIYLETPTLNFTKNQKISRKE
LEKEYFRGVYHEL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=420, Percent_Identity=22.8571428571429, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25141359, Length=298, Percent_Identity=25.8389261744966, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531443, Length=358, Percent_Identity=22.6256983240223, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130357, Length=221, Percent_Identity=27.6018099547511, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 47267; Mature: 47267

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNKFNFSKLAALFTDYPALKIGEISYSYQQINDLIQRKLTDYSHSSKTVITLINDSPL
CCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
NFIIDFLAIIESGNYPLVVDKSFKKKSFKFGLIPEDTLFLASTSGTTGPPKIYYRNWESW
HHHHHHHHHHHCCCCCEEEECCCCCCCCEECCCCCCCEEEEECCCCCCCCEEEEECCHHH
KMGFEVCDDLFHLSNSQALATTSPLTTSLGLHTLMCALYLGKTFLTITNPSQFINITENY
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCHHEEEEECCE
ALFTVPTYLLNNLNELFALTSPEIIFLGGGTLSPEAINKVRKKLPQTQMIEFYGSSETSF
EEEEEHHHHHHCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCHHEEHHEECCCCCCE
ISWQAVNEGKKTSSVGKLFPHVELTLGPKYRLTVKSPYLFSGYLNQPYPQSWTTDDLGTL
EEEEECCCCCCHHHHHHHCCEEEEEECCEEEEEECCCEEEECCCCCCCCCCCCCCCHHCC
KNNHLYLFGRRSDIIEHGANKIFPEEIERMAKDLCEDCIAFGVFDERYSQKIALLLLKPI
CCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEHCCCC
EKEKLIKILAQRLPKYKLPQIYLETPTLNFTKNQKISRKELEKEYFRGVYHEL
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKNKFNFSKLAALFTDYPALKIGEISYSYQQINDLIQRKLTDYSHSSKTVITLINDSPL
CCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
NFIIDFLAIIESGNYPLVVDKSFKKKSFKFGLIPEDTLFLASTSGTTGPPKIYYRNWESW
HHHHHHHHHHHCCCCCEEEECCCCCCCCEECCCCCCCEEEEECCCCCCCCEEEEECCHHH
KMGFEVCDDLFHLSNSQALATTSPLTTSLGLHTLMCALYLGKTFLTITNPSQFINITENY
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCHHEEEEECCE
ALFTVPTYLLNNLNELFALTSPEIIFLGGGTLSPEAINKVRKKLPQTQMIEFYGSSETSF
EEEEEHHHHHHCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCHHEEHHEECCCCCCE
ISWQAVNEGKKTSSVGKLFPHVELTLGPKYRLTVKSPYLFSGYLNQPYPQSWTTDDLGTL
EEEEECCCCCCHHHHHHHCCEEEEEECCEEEEEECCCEEEECCCCCCCCCCCCCCCHHCC
KNNHLYLFGRRSDIIEHGANKIFPEEIERMAKDLCEDCIAFGVFDERYSQKIALLLLKPI
CCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEHCCCC
EKEKLIKILAQRLPKYKLPQIYLETPTLNFTKNQKISRKELEKEYFRGVYHEL
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA