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The map label for this gene is yrfB

Identifier: 15673677

GI number: 15673677

Start: 1750521

End: 1751696

Strand: Direct

Name: yrfB

Synonym: L133858

Alternate gene names: 15673677

Gene position: 1750521-1751696 (Clockwise)

Preceding gene: 15673676

Following gene: 15673680

Centisome position: 74.0

GC content: 37.76

Gene sequence:

>1176_bases
ATGCCAAATCAATTAACTGATCATGTTACCTTACGTCATGGGGCAACTTTAAACAACCGTATTGTAATGTCTCCAATGCA
AACTCATTCTGGTTTAAAAGGTGGTTTCGCTTCTGATGATACTATTCAATATTACTCTGCCCGTTCACGAGCGGCAGGTA
TGTTAATTACAGAATTCCATTATGTAAGTGAAAATGGTGGACCAGCATATGTGCCAGGATATCCTGAACAATTAGGAGCT
TATTCTGACGCTCATCTTGAAGGACTACGAAAAACTGTACAGGCTCTAAAAAAAGATGGAAATAAAGCTATTCTTCAAAT
TCATCATGCTGGGCGAGCCGCTGTAGGACGTCATGTTAATGGATTAGATGTGGTGGCTCCTTCTGCTATCGATTTTTCTT
TCTTAGATTACCCTGTTCGTGAACTCACAATAACAGAGATTGATGATATTATTCTTGATTTTGGTAAAGCCACAAAACGG
GCAATTGATGCCGGCTTTGACGGTGTTGAAATTCATGGTGCCAATCATTATTTAATTCAACAATTTTTCTCAAAACTTTC
TAATCTTCGCACAGATAAATGGGGTGGTTCACTGAATAAACGAATGGCCTTTCCGCTAGCTGTTGTTAAAGAAGTCAAAG
CAATCGTCGATGAATACGCACCTAAAGGCTTTATCATTGGTTACCGTATTTCTCCTGAAGAACTTCATGGAAAAAATGTT
GGCTATGATTATACAGAAGCTCTAACACTTATCTCTGAAGTAATTAAATATGAACTTGATTATATCCATCTTTCATTATG
GGCAGGATACAATTCTAAACCTGAAAATAGCGAAAAATCTTATGCTGAACTTTTTAAGGAAATTTTAGATGATAAAACAA
AACTTCTTATTGTCGGTGGTATTTTTGGGGAGGATACAGCGACTGATGCGATTGAAAACTATGCTGATTTAATTGCCGTT
GCTCGTGGAACTTTGATTGATCCCAACTTTGCTAAAAAAGTTACTGAAGGAAAAGGGGACACAATTCTTCATAAGATTAG
TCCGGAAACAGTTGAGTATTCTCACTTAACTCCTGGATTACTTGAAGCCTTCAGCCGTGAAGATTCTCTTGGATTACCTC
CTTTACCCGGAGGCGAAACTATTAGACACTTGCATACAGGAAAGTATGATATCTAG

Upstream 100 bases:

>100_bases
GAGTTATAATTAACTTTCTTTTTACTTCTTCATATCGATATTTCTCGAATTATAGTTTTAATAATCTACTTTCCAAATAT
AATAATAACAGGATAAAAAT

Downstream 100 bases:

>100_bases
TTTTTTGATGACTAATATATATTAAAAGCTATTGTAACTTTACAGTAGTTTTTTCTTTTAAAATAAAAAAGTTCCTGACT
TAACAGGAACTTTATTTGGA

Product: NADH-dependent oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPNQLTDHVTLRHGATLNNRIVMSPMQTHSGLKGGFASDDTIQYYSARSRAAGMLITEFHYVSENGGPAYVPGYPEQLGA
YSDAHLEGLRKTVQALKKDGNKAILQIHHAGRAAVGRHVNGLDVVAPSAIDFSFLDYPVRELTITEIDDIILDFGKATKR
AIDAGFDGVEIHGANHYLIQQFFSKLSNLRTDKWGGSLNKRMAFPLAVVKEVKAIVDEYAPKGFIIGYRISPEELHGKNV
GYDYTEALTLISEVIKYELDYIHLSLWAGYNSKPENSEKSYAELFKEILDDKTKLLIVGGIFGEDTATDAIENYADLIAV
ARGTLIDPNFAKKVTEGKGDTILHKISPETVEYSHLTPGLLEAFSREDSLGLPPLPGGETIRHLHTGKYDI

Sequences:

>Translated_391_residues
MPNQLTDHVTLRHGATLNNRIVMSPMQTHSGLKGGFASDDTIQYYSARSRAAGMLITEFHYVSENGGPAYVPGYPEQLGA
YSDAHLEGLRKTVQALKKDGNKAILQIHHAGRAAVGRHVNGLDVVAPSAIDFSFLDYPVRELTITEIDDIILDFGKATKR
AIDAGFDGVEIHGANHYLIQQFFSKLSNLRTDKWGGSLNKRMAFPLAVVKEVKAIVDEYAPKGFIIGYRISPEELHGKNV
GYDYTEALTLISEVIKYELDYIHLSLWAGYNSKPENSEKSYAELFKEILDDKTKLLIVGGIFGEDTATDAIENYADLIAV
ARGTLIDPNFAKKVTEGKGDTILHKISPETVEYSHLTPGLLEAFSREDSLGLPPLPGGETIRHLHTGKYDI
>Mature_390_residues
PNQLTDHVTLRHGATLNNRIVMSPMQTHSGLKGGFASDDTIQYYSARSRAAGMLITEFHYVSENGGPAYVPGYPEQLGAY
SDAHLEGLRKTVQALKKDGNKAILQIHHAGRAAVGRHVNGLDVVAPSAIDFSFLDYPVRELTITEIDDIILDFGKATKRA
IDAGFDGVEIHGANHYLIQQFFSKLSNLRTDKWGGSLNKRMAFPLAVVKEVKAIVDEYAPKGFIIGYRISPEELHGKNVG
YDYTEALTLISEVIKYELDYIHLSLWAGYNSKPENSEKSYAELFKEILDDKTKLLIVGGIFGEDTATDAIENYADLIAVA
RGTLIDPNFAKKVTEGKGDTILHKISPETVEYSHLTPGLLEAFSREDSLGLPPLPGGETIRHLHTGKYDI

Specific function: Unknown

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=348, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789463, Length=335, Percent_Identity=27.4626865671642, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=417, Percent_Identity=26.8585131894484, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17564188, Length=367, Percent_Identity=26.9754768392371, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=262, Percent_Identity=30.1526717557252, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=259, Percent_Identity=29.7297297297297, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=310, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=365, Percent_Identity=23.013698630137, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=206, Percent_Identity=32.0388349514563, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6325086, Length=230, Percent_Identity=30, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 43102; Mature: 42971

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQLTDHVTLRHGATLNNRIVMSPMQTHSGLKGGFASDDTIQYYSARSRAAGMLITEFH
CCCCCCCCEEEECCCCCCCEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEE
YVSENGGPAYVPGYPEQLGAYSDAHLEGLRKTVQALKKDGNKAILQIHHAGRAAVGRHVN
EEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCC
GLDVVAPSAIDFSFLDYPVRELTITEIDDIILDFGKATKRAIDAGFDGVEIHGANHYLIQ
CCEEECCCCCCHHHHCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
QFFSKLSNLRTDKWGGSLNKRMAFPLAVVKEVKAIVDEYAPKGFIIGYRISPEELHGKNV
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCC
GYDYTEALTLISEVIKYELDYIHLSLWAGYNSKPENSEKSYAELFKEILDDKTKLLIVGG
CCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
IFGEDTATDAIENYADLIAVARGTLIDPNFAKKVTEGKGDTILHKISPETVEYSHLTPGL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCCEECCCCCHHH
LEAFSREDSLGLPPLPGGETIRHLHTGKYDI
HHHHCCCCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
PNQLTDHVTLRHGATLNNRIVMSPMQTHSGLKGGFASDDTIQYYSARSRAAGMLITEFH
CCCCCCCEEEECCCCCCCEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEE
YVSENGGPAYVPGYPEQLGAYSDAHLEGLRKTVQALKKDGNKAILQIHHAGRAAVGRHVN
EEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCC
GLDVVAPSAIDFSFLDYPVRELTITEIDDIILDFGKATKRAIDAGFDGVEIHGANHYLIQ
CCEEECCCCCCHHHHCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
QFFSKLSNLRTDKWGGSLNKRMAFPLAVVKEVKAIVDEYAPKGFIIGYRISPEELHGKNV
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCC
GYDYTEALTLISEVIKYELDYIHLSLWAGYNSKPENSEKSYAELFKEILDDKTKLLIVGG
CCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
IFGEDTATDAIENYADLIAVARGTLIDPNFAKKVTEGKGDTILHKISPETVEYSHLTPGL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCCEECCCCCHHH
LEAFSREDSLGLPPLPGGETIRHLHTGKYDI
HHHHCCCCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]