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The map label for this gene is eutD

Identifier: 15673641

GI number: 15673641

Start: 1708048

End: 1709028

Strand: Reverse

Name: eutD

Synonym: L107797

Alternate gene names: 15673641

Gene position: 1709028-1708048 (Counterclockwise)

Preceding gene: 15673642

Following gene: 15673640

Centisome position: 72.25

GC content: 38.33

Gene sequence:

>981_bases
ATGGAACTTTTTGAATCACTGAAGCAAAAAATTAGTGGAAAAGGCTTAAAAATTGTCTTTCCTGAAGGAACTGATGCACG
TATTTTAGGTGCTGCAAATCGTCTTAACGCTGATGGACTAATTACACCTGTCTTTATTGGAAATGTAAAAGAAGTCACTG
AAACATTGATTTCACGTGGAATTAATCCTGAAGGCTTTGAGATTTATGACCCAGAAAATTGTGGACGTTTTGAAACGATG
ACGGAAACTTTTGTGGAACGTCGTAAAGGTAAAGTTACAGAAGAACAAGCAAAAGAGCTCTTGAAAGACCCAAATTATTT
TGGAACGATGCTTGTTTATATGAAAATTGCTGACGGAATGGTTTCTGGCGCAATTCATTCAACTGCTGATACTGTTCGTC
CGGCCCTTCAAATCATTAAAACGAAACCAGGAGTAAAATCTGTTTCTGGTGCTTTTATTATGGTTCGTGGTCGTGATGAC
AATGAAAAATATATTTTTGGTGACTGTGCGATTAACATCAAACCAGATGCACCAACTCTTGCCGATATTGCTGTAGCTTC
AGCCGAAACAGCTGCACTCTTTGATATTGACCCCAAAGTTGCCATGCTTTCATTCTCAACTAAAGGTTCTGGAAAATCAG
AAGATGTTGATAAAGTAGTAGAAGCGACAAAACTTGTTAAAGAAGATTACCCTGAAATTGAAATTGATGGTGAACTTCAA
TTTGACGCTGCTTTTGTTCCAGCGGTGGCTCGTCAAAAAGCACCAGATTCAACTGTTGCTGGTCGTGCCAATGTCTTTAT
CTTCCCTGATATTCAATCAGGAAATATTGGATATAAAATTGCTCAACGATTGGGTAATTTTGAAGCGATTGGGCCAATTT
TGCAAGGTTTGAACGCACCTGTTTCTGACCTTTCTCGTGGAAGTAACGAAGAAGATGTCTATAAACTTGCAATTATCACA
GCTGCCCAAGCTTTAAAATAA

Upstream 100 bases:

>100_bases
TGAAAGTATGATATACTTAATTCGTAACAATTGCAGAAAGGAAATCCGCAACTTAAAGCGGTGGGTTATGAGAAATTTGT
AACCAAGGGATTTTGTCAAA

Downstream 100 bases:

>100_bases
ATAATGATATTCTGTCAGTAAATTTGTAAATGAATAATTACTGACAAGTCTGTCATTAATCCGCCATTTGAGGCGGATTT
TTTTGTTGGTCTAAAAATGT

Product: phosphotransacetylase

Products: NA

Alternate protein names: Phosphotransacetylase; Vegetative protein 43; VEG43 [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MELFESLKQKISGKGLKIVFPEGTDARILGAANRLNADGLITPVFIGNVKEVTETLISRGINPEGFEIYDPENCGRFETM
TETFVERRKGKVTEEQAKELLKDPNYFGTMLVYMKIADGMVSGAIHSTADTVRPALQIIKTKPGVKSVSGAFIMVRGRDD
NEKYIFGDCAINIKPDAPTLADIAVASAETAALFDIDPKVAMLSFSTKGSGKSEDVDKVVEATKLVKEDYPEIEIDGELQ
FDAAFVPAVARQKAPDSTVAGRANVFIFPDIQSGNIGYKIAQRLGNFEAIGPILQGLNAPVSDLSRGSNEEDVYKLAIIT
AAQALK

Sequences:

>Translated_326_residues
MELFESLKQKISGKGLKIVFPEGTDARILGAANRLNADGLITPVFIGNVKEVTETLISRGINPEGFEIYDPENCGRFETM
TETFVERRKGKVTEEQAKELLKDPNYFGTMLVYMKIADGMVSGAIHSTADTVRPALQIIKTKPGVKSVSGAFIMVRGRDD
NEKYIFGDCAINIKPDAPTLADIAVASAETAALFDIDPKVAMLSFSTKGSGKSEDVDKVVEATKLVKEDYPEIEIDGELQ
FDAAFVPAVARQKAPDSTVAGRANVFIFPDIQSGNIGYKIAQRLGNFEAIGPILQGLNAPVSDLSRGSNEEDVYKLAIIT
AAQALK
>Mature_326_residues
MELFESLKQKISGKGLKIVFPEGTDARILGAANRLNADGLITPVFIGNVKEVTETLISRGINPEGFEIYDPENCGRFETM
TETFVERRKGKVTEEQAKELLKDPNYFGTMLVYMKIADGMVSGAIHSTADTVRPALQIIKTKPGVKSVSGAFIMVRGRDD
NEKYIFGDCAINIKPDAPTLADIAVASAETAALFDIDPKVAMLSFSTKGSGKSEDVDKVVEATKLVKEDYPEIEIDGELQ
FDAAFVPAVARQKAPDSTVAGRANVFIFPDIQSGNIGYKIAQRLGNFEAIGPILQGLNAPVSDLSRGSNEEDVYKLAIIT
AAQALK

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=312, Percent_Identity=47.1153846153846, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI1788800, Length=309, Percent_Identity=44.336569579288, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1788806, Length=298, Percent_Identity=32.5503355704698, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR004614
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 35257; Mature: 35257

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFESLKQKISGKGLKIVFPEGTDARILGAANRLNADGLITPVFIGNVKEVTETLISRG
CHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHCC
INPEGFEIYDPENCGRFETMTETFVERRKGKVTEEQAKELLKDPNYFGTMLVYMKIADGM
CCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSGAIHSTADTVRPALQIIKTKPGVKSVSGAFIMVRGRDDNEKYIFGDCAINIKPDAPTL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCH
ADIAVASAETAALFDIDPKVAMLSFSTKGSGKSEDVDKVVEATKLVKEDYPEIEIDGELQ
HHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCEEE
FDAAFVPAVARQKAPDSTVAGRANVFIFPDIQSGNIGYKIAQRLGNFEAIGPILQGLNAP
ECHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCC
VSDLSRGSNEEDVYKLAIITAAQALK
HHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELFESLKQKISGKGLKIVFPEGTDARILGAANRLNADGLITPVFIGNVKEVTETLISRG
CHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHCC
INPEGFEIYDPENCGRFETMTETFVERRKGKVTEEQAKELLKDPNYFGTMLVYMKIADGM
CCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSGAIHSTADTVRPALQIIKTKPGVKSVSGAFIMVRGRDDNEKYIFGDCAINIKPDAPTL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCH
ADIAVASAETAALFDIDPKVAMLSFSTKGSGKSEDVDKVVEATKLVKEDYPEIEIDGELQ
HHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCEEE
FDAAFVPAVARQKAPDSTVAGRANVFIFPDIQSGNIGYKIAQRLGNFEAIGPILQGLNAP
ECHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCC
VSDLSRGSNEEDVYKLAIITAAQALK
HHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9298659 [H]