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The map label for this gene is rbsB

Identifier: 15673617

GI number: 15673617

Start: 1682562

End: 1683536

Strand: Reverse

Name: rbsB

Synonym: L82310

Alternate gene names: 15673617

Gene position: 1683536-1682562 (Counterclockwise)

Preceding gene: 15673618

Following gene: 15673616

Centisome position: 71.17

GC content: 37.13

Gene sequence:

>975_bases
ATGAAATTAGTAAAAAAATTAACTTTTGCTTTTGTAGCTACTTTAGCAGTCTCAACTTTGACCGCATGTAGTCTTTATAC
CGGTATTCCAGGCCGTGATGGAAAGGAAAAAGTAGAAGAGGTAACGCATAAGAAACCAAATGAATTAAATATTGGTGTTT
CTATTTCGACAACAAACAATCCTTATTTTGTTTCAATGAACAATGCGATTAAATCAATGGCCAAAGAAAAAAATACAAAA
TTAACGGTTTCTGATGCCCAAAATGATTCTGCAACACAATTAAATAATGTTCAAAATTTCATCAGCCAAAATGTGGATGC
GATTTTAATTAATCCTGTAGATTCTGATGCCATTGTTCCAGCCATTAAAGCAGCCAATAATGCCCATATCCCAGTGATTG
CAATGGATAGAGGATCAAATGGTGGAACAGTTTTAACAACCGTTGCCTCCGATAATGTGGCAGCTGGGAAAATGGCGGCT
CAAGCAGTTGAAAAATTGGTTGGGAAAAACGTAAAAGTTCTGGAACTTTCTGGAACGCCAGGGGCTTCGGCAACAATAGA
CCGTGGTAAAGGGTTTAATAGTGAAGCTAAGACCTTAGGTTTAAATATATTATCCAGTCAATCAGCTAATTTTGACAGAA
CAACTGGTTTAAATACGACTCAAAATATGCTCCAATCTCATAAAGACGTCCAAGCAATCTTTGCGCAAAATGATGAAATG
GCACTGGGAGCAGCTAAAGCTGTGCAAGCCACTGGACAAAAAATAGCTATTTTTGGAATTGATGGTGAGTCTGAAACACA
TGATGCCATAAAAGCAGGGACAATGACCGCAACGATTGCTCAACAACCAGCTAAAATTGGTGAAATGGCTTTGCAAGCTA
CCTATGATTATTATCAAGGTAAAAAAATTGCTCATATGATAAATTCGCCAATCTATCTTGTGGATAAAGATAATGTTGAC
CAACATCAAAGGTAG

Upstream 100 bases:

>100_bases
GTGTGTCAGCCTTTTGGCAACAAGTCATCCAAGGATTAGTTATTCTCGTTGCAGTTCTTATCGATGTCATTCGTAGCAAA
CGGAAATAGGAGAGTTGAAC

Downstream 100 bases:

>100_bases
TATAAAAATATAAATTCTGTCGCTAGGACAGAGTTTTTTTATTTTTAAAAATAAAGATGAATAATGAATAAAAATATAAA
TTTGTCCAAATGACATTTTT

Product: ribose ABC transporter substrate binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLVKKLTFAFVATLAVSTLTACSLYTGIPGRDGKEKVEEVTHKKPNELNIGVSISTTNNPYFVSMNNAIKSMAKEKNTK
LTVSDAQNDSATQLNNVQNFISQNVDAILINPVDSDAIVPAIKAANNAHIPVIAMDRGSNGGTVLTTVASDNVAAGKMAA
QAVEKLVGKNVKVLELSGTPGASATIDRGKGFNSEAKTLGLNILSSQSANFDRTTGLNTTQNMLQSHKDVQAIFAQNDEM
ALGAAKAVQATGQKIAIFGIDGESETHDAIKAGTMTATIAQQPAKIGEMALQATYDYYQGKKIAHMINSPIYLVDKDNVD
QHQR

Sequences:

>Translated_324_residues
MKLVKKLTFAFVATLAVSTLTACSLYTGIPGRDGKEKVEEVTHKKPNELNIGVSISTTNNPYFVSMNNAIKSMAKEKNTK
LTVSDAQNDSATQLNNVQNFISQNVDAILINPVDSDAIVPAIKAANNAHIPVIAMDRGSNGGTVLTTVASDNVAAGKMAA
QAVEKLVGKNVKVLELSGTPGASATIDRGKGFNSEAKTLGLNILSSQSANFDRTTGLNTTQNMLQSHKDVQAIFAQNDEM
ALGAAKAVQATGQKIAIFGIDGESETHDAIKAGTMTATIAQQPAKIGEMALQATYDYYQGKKIAHMINSPIYLVDKDNVD
QHQR
>Mature_324_residues
MKLVKKLTFAFVATLAVSTLTACSLYTGIPGRDGKEKVEEVTHKKPNELNIGVSISTTNNPYFVSMNNAIKSMAKEKNTK
LTVSDAQNDSATQLNNVQNFISQNVDAILINPVDSDAIVPAIKAANNAHIPVIAMDRGSNGGTVLTTVASDNVAAGKMAA
QAVEKLVGKNVKVLELSGTPGASATIDRGKGFNSEAKTLGLNILSSQSANFDRTTGLNTTQNMLQSHKDVQAIFAQNDEM
ALGAAKAVQATGQKIAIFGIDGESETHDAIKAGTMTATIAQQPAKIGEMALQATYDYYQGKKIAHMINSPIYLVDKDNVD
QHQR

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=254, Percent_Identity=43.7007874015748, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1790674, Length=282, Percent_Identity=31.5602836879433, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790526, Length=283, Percent_Identity=30.7420494699647, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34380; Mature: 34380

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVKKLTFAFVATLAVSTLTACSLYTGIPGRDGKEKVEEVTHKKPNELNIGVSISTTNN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCC
PYFVSMNNAIKSMAKEKNTKLTVSDAQNDSATQLNNVQNFISQNVDAILINPVDSDAIVP
CEEEEECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
AIKAANNAHIPVIAMDRGSNGGTVLTTVASDNVAAGKMAAQAVEKLVGKNVKVLELSGTP
HHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
GASATIDRGKGFNSEAKTLGLNILSSQSANFDRTTGLNTTQNMLQSHKDVQAIFAQNDEM
CCCCEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
ALGAAKAVQATGQKIAIFGIDGESETHDAIKAGTMTATIAQQPAKIGEMALQATYDYYQG
HHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCEEEHHHHCCHHHHHHHHHHHHHHHCC
KKIAHMINSPIYLVDKDNVDQHQR
CHHHHHHCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKLVKKLTFAFVATLAVSTLTACSLYTGIPGRDGKEKVEEVTHKKPNELNIGVSISTTNN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCC
PYFVSMNNAIKSMAKEKNTKLTVSDAQNDSATQLNNVQNFISQNVDAILINPVDSDAIVP
CEEEEECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
AIKAANNAHIPVIAMDRGSNGGTVLTTVASDNVAAGKMAAQAVEKLVGKNVKVLELSGTP
HHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
GASATIDRGKGFNSEAKTLGLNILSSQSANFDRTTGLNTTQNMLQSHKDVQAIFAQNDEM
CCCCEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
ALGAAKAVQATGQKIAIFGIDGESETHDAIKAGTMTATIAQQPAKIGEMALQATYDYYQG
HHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCEEEHHHHCCHHHHHHHHHHHHHHHCC
KKIAHMINSPIYLVDKDNVDQHQR
CHHHHHHCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]