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The map label for this gene is ypfE

Identifier: 15673498

GI number: 15673498

Start: 1556660

End: 1558048

Strand: Direct

Name: ypfE

Synonym: L156385

Alternate gene names: 15673498

Gene position: 1556660-1558048 (Clockwise)

Preceding gene: 15673497

Following gene: 15673503

Centisome position: 65.8

GC content: 37.15

Gene sequence:

>1389_bases
ATGAATAATACTAATGATTTTAATTTAAAAAAAGTCTTACCTGCGGTTCTAACAATTGCAATTGGAATGCTTTTAGTAAT
GATGGATACAACCATTATGAATGTCAGTCTTCCGCATATCCAGTCTGCTTTCCATCAAAATCTCTCAAATAGTCAGTGGG
TCATTACTGCTTATACTTTAGCTATGGCCACAGTTATTCCATTTGCTGGATTTTTGGGAGACCGCTTTTCAGATAAAAAA
GTTTTTGCTTTAGCAATCATTTTTTTCACTATCGCTTCATTTCTTGCAGCTAACGCTCACTCACTGCAAAGTCTTATTAT
TTGGCGAATTGCTCAAGGCTTAACTGGTGGCGTTGTTGCTCCAATCGGAATTGGGATGTCTTTTAAAATTATCCCAATGG
AAAAACGAGGTTCAATGATGGGCCTTCTAGGGTTACCAATGTTGCTCGCTCCAACAATAGGACCTGCACTTTCGGGTTTC
TTAGTTAAACACTTCAATTGGTCAACTGTCTTTTTGATTAATCTTCCGGTTGGAGTTCTAGCTCTAATCTTTGTTCTCCT
CTTTCTCCCCAATTTTCCTGCAAATAAAGCAAGTAAAATTGACTTAAAAGGGACTCTTCTTTCTCCATTTGCCTTTCCAA
TTTTAATTTATGGTGTACACGTTGGGACAGATAAAGGCTGGTCAAATCCTACTGCCCTCTCTTTTGTAGGCCTTGGTATA
ATCATGTTGCTTTTATTCATTATTGTTGAACTACGTGTTGAAAATCCTCTTTTACACCTTCGAGCTTTTGCAATTCCTGA
ATTTACAAAAGGAATTTCGCTCATGTGGCTCAATCAAATCGTTGTTTTCGGGGCCATGCTTCTTGTCCCACTTTACTTAC
AAAATGTCGTTGGACTTTCTTCTGAAAATACAGGGCTAATCATGGTTCCGCAGGCAATTGCTAGTTTTTTAGGAATGACA
ATCGGTGGACGTATTTTTGATAAATTTGGGACGAAAGCCGCAGTTCTGCCGGGCTTTATTCTGGGAGCTCTAAGTCTTAG
TCTTTTTAGTCAAATTAAACCTAGTTCTTCTTTGGCTTTCACACTTTGTGCTATTGTTTTACTTGGTTTAAGTCAAGGTT
TAGTCAATATGCAAGTTAATAATCACGCCCTTCAATCTGTTCCAATGAAAAATATCAGTCGAGTTACTCCACTAACAAAT
GAAATGATGCAAGTAGTCAACTCATTTGCCATTGCCTTTTTGACAGCATTTTTAAGTGGTCAAATCAAAAAACAGACTGG
ACTTTCTCCTTTACAAGCGAATCTAACTGCCTTCCATCATACTTTCTACTTACTTCTTATTTTTGTAAGCTTCGGTTTCA
TTTTAAGTCTATTTTTGAGAAAAAAGTGA

Upstream 100 bases:

>100_bases
ATAAAAAACTTCCTGAGGAAGTTTTTTTCTTGACTTTATTTCAGAACAATGGTATTATTTTTAACGAACAGTGTTCTAAA
AATATAAAAGAAGATTCTTT

Downstream 100 bases:

>100_bases
AGGCATAATAAAAAAACACTGTTAAAATAAACAGTGTTTTTTATTACCAATTTTTAACCAAAGCTGGAATTTCTAATAGG
TCTTGGATTTGCACATCTGG

Product: transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNNTNDFNLKKVLPAVLTIAIGMLLVMMDTTIMNVSLPHIQSAFHQNLSNSQWVITAYTLAMATVIPFAGFLGDRFSDKK
VFALAIIFFTIASFLAANAHSLQSLIIWRIAQGLTGGVVAPIGIGMSFKIIPMEKRGSMMGLLGLPMLLAPTIGPALSGF
LVKHFNWSTVFLINLPVGVLALIFVLLFLPNFPANKASKIDLKGTLLSPFAFPILIYGVHVGTDKGWSNPTALSFVGLGI
IMLLLFIIVELRVENPLLHLRAFAIPEFTKGISLMWLNQIVVFGAMLLVPLYLQNVVGLSSENTGLIMVPQAIASFLGMT
IGGRIFDKFGTKAAVLPGFILGALSLSLFSQIKPSSSLAFTLCAIVLLGLSQGLVNMQVNNHALQSVPMKNISRVTPLTN
EMMQVVNSFAIAFLTAFLSGQIKKQTGLSPLQANLTAFHHTFYLLLIFVSFGFILSLFLRKK

Sequences:

>Translated_462_residues
MNNTNDFNLKKVLPAVLTIAIGMLLVMMDTTIMNVSLPHIQSAFHQNLSNSQWVITAYTLAMATVIPFAGFLGDRFSDKK
VFALAIIFFTIASFLAANAHSLQSLIIWRIAQGLTGGVVAPIGIGMSFKIIPMEKRGSMMGLLGLPMLLAPTIGPALSGF
LVKHFNWSTVFLINLPVGVLALIFVLLFLPNFPANKASKIDLKGTLLSPFAFPILIYGVHVGTDKGWSNPTALSFVGLGI
IMLLLFIIVELRVENPLLHLRAFAIPEFTKGISLMWLNQIVVFGAMLLVPLYLQNVVGLSSENTGLIMVPQAIASFLGMT
IGGRIFDKFGTKAAVLPGFILGALSLSLFSQIKPSSSLAFTLCAIVLLGLSQGLVNMQVNNHALQSVPMKNISRVTPLTN
EMMQVVNSFAIAFLTAFLSGQIKKQTGLSPLQANLTAFHHTFYLLLIFVSFGFILSLFLRKK
>Mature_462_residues
MNNTNDFNLKKVLPAVLTIAIGMLLVMMDTTIMNVSLPHIQSAFHQNLSNSQWVITAYTLAMATVIPFAGFLGDRFSDKK
VFALAIIFFTIASFLAANAHSLQSLIIWRIAQGLTGGVVAPIGIGMSFKIIPMEKRGSMMGLLGLPMLLAPTIGPALSGF
LVKHFNWSTVFLINLPVGVLALIFVLLFLPNFPANKASKIDLKGTLLSPFAFPILIYGVHVGTDKGWSNPTALSFVGLGI
IMLLLFIIVELRVENPLLHLRAFAIPEFTKGISLMWLNQIVVFGAMLLVPLYLQNVVGLSSENTGLIMVPQAIASFLGMT
IGGRIFDKFGTKAAVLPGFILGALSLSLFSQIKPSSSLAFTLCAIVLLGLSQGLVNMQVNNHALQSVPMKNISRVTPLTN
EMMQVVNSFAIAFLTAFLSGQIKKQTGLSPLQANLTAFHHTFYLLLIFVSFGFILSLFLRKK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=26.0047281323877, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788710, Length=331, Percent_Identity=29.0030211480363, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789042, Length=411, Percent_Identity=26.2773722627737, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081983, Length=396, Percent_Identity=26.2626262626263, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788392, Length=397, Percent_Identity=27.7078085642317, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325455, Length=242, Percent_Identity=26.4462809917355, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=376, Percent_Identity=21.8085106382979, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=334, Percent_Identity=24.251497005988, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50142; Mature: 50142

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTNDFNLKKVLPAVLTIAIGMLLVMMDTTIMNVSLPHIQSAFHQNLSNSQWVITAYTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHH
AMATVIPFAGFLGDRFSDKKVFALAIIFFTIASFLAANAHSLQSLIIWRIAQGLTGGVVA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCH
PIGIGMSFKIIPMEKRGSMMGLLGLPMLLAPTIGPALSGFLVKHFNWSTVFLINLPVGVL
HCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
ALIFVLLFLPNFPANKASKIDLKGTLLSPFAFPILIYGVHVGTDKGWSNPTALSFVGLGI
HHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHH
IMLLLFIIVELRVENPLLHLRAFAIPEFTKGISLMWLNQIVVFGAMLLVPLYLQNVVGLS
HHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SENTGLIMVPQAIASFLGMTIGGRIFDKFGTKAAVLPGFILGALSLSLFSQIKPSSSLAF
CCCCCEEEEHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TLCAIVLLGLSQGLVNMQVNNHALQSVPMKNISRVTPLTNEMMQVVNSFAIAFLTAFLSG
HHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
QIKKQTGLSPLQANLTAFHHTFYLLLIFVSFGFILSLFLRKK
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNTNDFNLKKVLPAVLTIAIGMLLVMMDTTIMNVSLPHIQSAFHQNLSNSQWVITAYTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHH
AMATVIPFAGFLGDRFSDKKVFALAIIFFTIASFLAANAHSLQSLIIWRIAQGLTGGVVA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCH
PIGIGMSFKIIPMEKRGSMMGLLGLPMLLAPTIGPALSGFLVKHFNWSTVFLINLPVGVL
HCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
ALIFVLLFLPNFPANKASKIDLKGTLLSPFAFPILIYGVHVGTDKGWSNPTALSFVGLGI
HHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHH
IMLLLFIIVELRVENPLLHLRAFAIPEFTKGISLMWLNQIVVFGAMLLVPLYLQNVVGLS
HHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SENTGLIMVPQAIASFLGMTIGGRIFDKFGTKAAVLPGFILGALSLSLFSQIKPSSSLAF
CCCCCEEEEHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TLCAIVLLGLSQGLVNMQVNNHALQSVPMKNISRVTPLTNEMMQVVNSFAIAFLTAFLSG
HHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
QIKKQTGLSPLQANLTAFHHTFYLLLIFVSFGFILSLFLRKK
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]