Click here to switch to the map view.

The map label for this gene is vacB2

Identifier: 15673187

GI number: 15673187

Start: 1226204

End: 1228201

Strand: Direct

Name: vacB2

Synonym: L25961

Alternate gene names: 15673187

Gene position: 1226204-1228201 (Clockwise)

Preceding gene: 15673186

Following gene: 15673188

Centisome position: 51.84

GC content: 35.09

Gene sequence:

>1998_bases
ATGGTTCAGCTATCAGAATTAGCATCAGCACTTAATCAAACAGAAAGTAAGGGAGTATTTTCTAAACATCCTAAAGGTTT
CGGATTTGTTCATCCAGAAGACGCAACAGATAAAACAAATGATATCTATATCGGTAAAAATGATACTAAATTTGCGATGG
ATGGGGATAAAGTTACCGTTAAAGTTACCTATCCTAAAACTGAAAAACGCGGTGCAAGTGGACAAATTACTAAAATAAAT
GAACGAGCCGTAGTTGATACGGTCGGGACTTACCGTTCTTTGTCAAATCGTCAAGTCAAAGCATTAGGATACAAAGGAAG
AATAGAACTCTATAATGATCGAATTTCTGATACTTTATATATCAAACAACCTTTATCAGGGGTTCAAGAAGAAGATGTAG
TAAGTCTTAAAATAACCCAATATCCAACCAATACCAAGACATTTGAAGGAAAAATAACAGGTATTATTGGACATAAAGGC
GAAGTAGGACTAGATATTTTAGAAGTGCTTTGTGCTATGAAAATTCCACAAGAATTCTCTTCTGAAACTTTAGCTGAGGC
TGAGGCATTTTCCGAAAAATTAACTGATTCAGATTTGCAAGATCGTGAAGATTATCGGAATGAAATTACCTATACCATTG
ATGGAGATGATTCTAAGGATTTGGATGATGCCATCCACGTTAAAAAATTATCAAATTGGCATTTTGAATTAGGAGTCCAT
ATCGCTGACGTCAGTCATTATGTGACTGAGGGTTCTTCTTTAGATGAAGAAGCCTACTCACGCGCAACATCAGTCTATGT
AACTGACCGAGTTGTTCCGATGTTGCCTGTTAAGTTATCCAATAATTTGTGTTCACTTAATGAAGCTCAAGAACGACTGA
CAATGTCTTGTCTAATGGAAATTGATGACAAAGGTAAGATTGTCAGTTATAAAATATCACCATCAGTCATAAAAACAACT
TATCGAATGACTTATAATAATGTCAATAAAATGATTCATCAAGGACAAGAAGGTCATAGGGAAGCCCTTGAAAACTTCTT
TAAAATTACCGATTCGATTAAAGTGGCCGTTGAACTTCATGAAATTCTTGAAACAATGCGAAAAGACAGAGGAATGATTG
AATTTGACGAGAGTGAAGCAAAAATTATTTTAGATGAAAAAGGTCATCCAATCGAAATTGTAAAACGTGATCGTGATACT
GCAGAACGAATGATTGAATCCTTTATGTTGATGGCAAATGAAACTGTTGCCCTTGATTTTCAAAAGAAAAAACTACCTTC
CTTATATCGAGTTCACGATAACCCTAAAGAGAAATCTTTTGCTAAATTGATGGAAGCTGCTGCTAATGCTGGTTTTTCCT
TGAATTCAGATTCTCATCAAGCCATTAATTTCTTTGCTGATGAAATTAAAGGAACAAGTTCTGAAAAGGCATTGACTTAT
CAACTTCGACATACGATGTCAACAGCGGTTTACTCTGAAAAAAATACAAAACATTTTGGGCTCGCAGCGACTAACTATAC
GCATTTCACAAGCCCAATTAGACGTTATCCTGATTTAATCATCCATCGTTTGTTGCATTTATATCCTTCTGACCATTCTA
ACCATACTAAAGACGAGTGGAAAGAACGTTTACCTGAAATTGCTAGCCATTCTTCTGATATGGAACACCGTGCGGTCGTC
ACTGAACGAATTATTGATGCAATGAAAAAAGCAGAATATATGTCAGAGCGGATTGGAGAAGTTTACACTGGTACAATTAC
AGGCCTGCAAAAATTTGGAATCTTTGTTGCATTAGATAATACAGTTGAAGGTTTAATTCGTGTACCTAATTTACATACTG
GGACTACGGAAGAACTTGAATTTGATGAAGAAGCAAGTATTTTTAAAGGAAAAAAATCAGAAACAGTCTATCAAATAGGA
CAAGAAATAAAAATTCGTGTTATTGCTGCTAATAAACGAAAAGGAACAGTTGATTTTGAACAAATTGCTCCTGAATAA

Upstream 100 bases:

>100_bases
ATAAAAGGCTATCAAAGGCCTTTTTATTATTAATAGTCGAATATTGCTTACTGATTATGTTATGATAGAAAGATACAATT
GGAGGAAGAAATAAATATAT

Downstream 100 bases:

>100_bases
AGCCAGTCTACTATTATGTCGAATAAATAAAGGACTAATAAGATAAATTAAACTATTGAAATTTAAATATTTTCGAGTAA
AATACCAGTATTTACTGGTA

Product: ribonuclease

Products: NA

Alternate protein names: RNase R 2; VacB protein homolog 2

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MVQLSELASALNQTESKGVFSKHPKGFGFVHPEDATDKTNDIYIGKNDTKFAMDGDKVTVKVTYPKTEKRGASGQITKIN
ERAVVDTVGTYRSLSNRQVKALGYKGRIELYNDRISDTLYIKQPLSGVQEEDVVSLKITQYPTNTKTFEGKITGIIGHKG
EVGLDILEVLCAMKIPQEFSSETLAEAEAFSEKLTDSDLQDREDYRNEITYTIDGDDSKDLDDAIHVKKLSNWHFELGVH
IADVSHYVTEGSSLDEEAYSRATSVYVTDRVVPMLPVKLSNNLCSLNEAQERLTMSCLMEIDDKGKIVSYKISPSVIKTT
YRMTYNNVNKMIHQGQEGHREALENFFKITDSIKVAVELHEILETMRKDRGMIEFDESEAKIILDEKGHPIEIVKRDRDT
AERMIESFMLMANETVALDFQKKKLPSLYRVHDNPKEKSFAKLMEAAANAGFSLNSDSHQAINFFADEIKGTSSEKALTY
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNHTKDEWKERLPEIASHSSDMEHRAVV
TERIIDAMKKAEYMSERIGEVYTGTITGLQKFGIFVALDNTVEGLIRVPNLHTGTTEELEFDEEASIFKGKKSETVYQIG
QEIKIRVIAANKRKGTVDFEQIAPE

Sequences:

>Translated_665_residues
MVQLSELASALNQTESKGVFSKHPKGFGFVHPEDATDKTNDIYIGKNDTKFAMDGDKVTVKVTYPKTEKRGASGQITKIN
ERAVVDTVGTYRSLSNRQVKALGYKGRIELYNDRISDTLYIKQPLSGVQEEDVVSLKITQYPTNTKTFEGKITGIIGHKG
EVGLDILEVLCAMKIPQEFSSETLAEAEAFSEKLTDSDLQDREDYRNEITYTIDGDDSKDLDDAIHVKKLSNWHFELGVH
IADVSHYVTEGSSLDEEAYSRATSVYVTDRVVPMLPVKLSNNLCSLNEAQERLTMSCLMEIDDKGKIVSYKISPSVIKTT
YRMTYNNVNKMIHQGQEGHREALENFFKITDSIKVAVELHEILETMRKDRGMIEFDESEAKIILDEKGHPIEIVKRDRDT
AERMIESFMLMANETVALDFQKKKLPSLYRVHDNPKEKSFAKLMEAAANAGFSLNSDSHQAINFFADEIKGTSSEKALTY
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNHTKDEWKERLPEIASHSSDMEHRAVV
TERIIDAMKKAEYMSERIGEVYTGTITGLQKFGIFVALDNTVEGLIRVPNLHTGTTEELEFDEEASIFKGKKSETVYQIG
QEIKIRVIAANKRKGTVDFEQIAPE
>Mature_665_residues
MVQLSELASALNQTESKGVFSKHPKGFGFVHPEDATDKTNDIYIGKNDTKFAMDGDKVTVKVTYPKTEKRGASGQITKIN
ERAVVDTVGTYRSLSNRQVKALGYKGRIELYNDRISDTLYIKQPLSGVQEEDVVSLKITQYPTNTKTFEGKITGIIGHKG
EVGLDILEVLCAMKIPQEFSSETLAEAEAFSEKLTDSDLQDREDYRNEITYTIDGDDSKDLDDAIHVKKLSNWHFELGVH
IADVSHYVTEGSSLDEEAYSRATSVYVTDRVVPMLPVKLSNNLCSLNEAQERLTMSCLMEIDDKGKIVSYKISPSVIKTT
YRMTYNNVNKMIHQGQEGHREALENFFKITDSIKVAVELHEILETMRKDRGMIEFDESEAKIILDEKGHPIEIVKRDRDT
AERMIESFMLMANETVALDFQKKKLPSLYRVHDNPKEKSFAKLMEAAANAGFSLNSDSHQAINFFADEIKGTSSEKALTY
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNHTKDEWKERLPEIASHSSDMEHRAVV
TERIIDAMKKAEYMSERIGEVYTGTITGLQKFGIFVALDNTVEGLIRVPNLHTGTTEELEFDEEASIFKGKKSETVYQIG
QEIKIRVIAANKRKGTVDFEQIAPE

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI190014625, Length=403, Percent_Identity=31.2655086848635, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI190014623, Length=403, Percent_Identity=31.2655086848635, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI134288890, Length=416, Percent_Identity=30.0480769230769, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI19115966, Length=557, Percent_Identity=26.5709156193896, Blast_Score=166, Evalue=9e-41,
Organism=Homo sapiens, GI219521928, Length=557, Percent_Identity=26.5709156193896, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI156105695, Length=183, Percent_Identity=29.5081967213115, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI156105693, Length=183, Percent_Identity=29.5081967213115, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI87082383, Length=662, Percent_Identity=29.607250755287, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787542, Length=666, Percent_Identity=23.5735735735736, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI212645896, Length=410, Percent_Identity=32.9268292682927, Blast_Score=195, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17553506, Length=454, Percent_Identity=27.9735682819383, Blast_Score=185, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6324552, Length=505, Percent_Identity=29.3069306930693, Blast_Score=174, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320499, Length=317, Percent_Identity=24.9211356466877, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323943, Length=375, Percent_Identity=22.9333333333333, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24649634, Length=432, Percent_Identity=31.712962962963, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24654592, Length=356, Percent_Identity=32.5842696629214, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI19922976, Length=356, Percent_Identity=32.5842696629214, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24654597, Length=356, Percent_Identity=32.5842696629214, Blast_Score=176, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNR2_LACLA (Q02146)

Other databases:

- EMBL:   AE005176
- EMBL:   U92974
- PIR:   A45734
- PIR:   E86775
- RefSeq:   NP_267361.1
- ProteinModelPortal:   Q02146
- SMR:   Q02146
- GeneID:   1114852
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L25961
- NMPDR:   fig|272623.1.peg.1240
- HOGENOM:   HBG690243
- OMA:   INERAVV
- ProtClustDB:   CLSK697627
- BioCyc:   LLAC272623:L25961-MONOMER
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR23355:SF9
- SMART:   SM00357
- SMART:   SM00955
- SMART:   SM00316
- TIGRFAMs:   TIGR00358
- TIGRFAMs:   TIGR02063

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 3.1.-.- [C]

Molecular weight: Translated: 75275; Mature: 75275

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01175 RIBONUCLEASE_II; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQLSELASALNQTESKGVFSKHPKGFGFVHPEDATDKTNDIYIGKNDTKFAMDGDKVTV
CCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCEEEE
KVTYPKTEKRGASGQITKINERAVVDTVGTYRSLSNRQVKALGYKGRIELYNDRISDTLY
EEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEE
IKQPLSGVQEEDVVSLKITQYPTNTKTFEGKITGIIGHKGEVGLDILEVLCAMKIPQEFS
EECCCCCCCCCCEEEEEEEECCCCCCEECCEEEEEECCCCCCCHHHHHHHHHHHCCHHHH
SETLAEAEAFSEKLTDSDLQDREDYRNEITYTIDGDDSKDLDDAIHVKKLSNWHFELGVH
HHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEE
IADVSHYVTEGSSLDEEAYSRATSVYVTDRVVPMLPVKLSNNLCSLNEAQERLTMSCLME
EEHHHHHHHCCCCCCHHHHHHHEEEEEECCCCEEECEECCCCCCCHHHHHHHHHHHHHHH
IDDKGKIVSYKISPSVIKTTYRMTYNNVNKMIHQGQEGHREALENFFKITDSIKVAVELH
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EILETMRKDRGMIEFDESEAKIILDEKGHPIEIVKRDRDTAERMIESFMLMANETVALDF
HHHHHHHHCCCCEEECCCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
QKKKLPSLYRVHDNPKEKSFAKLMEAAANAGFSLNSDSHQAINFFADEIKGTSSEKALTY
HHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNHTKDEW
HHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KERLPEIASHSSDMEHRAVVTERIIDAMKKAEYMSERIGEVYTGTITGLQKFGIFVALDN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEECC
TVEGLIRVPNLHTGTTEELEFDEEASIFKGKKSETVYQIGQEIKIRVIAANKRKGTVDFE
CHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCEEEEEEEEECCCCCCCCHH
QIAPE
HCCCC
>Mature Secondary Structure
MVQLSELASALNQTESKGVFSKHPKGFGFVHPEDATDKTNDIYIGKNDTKFAMDGDKVTV
CCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCEEEE
KVTYPKTEKRGASGQITKINERAVVDTVGTYRSLSNRQVKALGYKGRIELYNDRISDTLY
EEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEE
IKQPLSGVQEEDVVSLKITQYPTNTKTFEGKITGIIGHKGEVGLDILEVLCAMKIPQEFS
EECCCCCCCCCCEEEEEEEECCCCCCEECCEEEEEECCCCCCCHHHHHHHHHHHCCHHHH
SETLAEAEAFSEKLTDSDLQDREDYRNEITYTIDGDDSKDLDDAIHVKKLSNWHFELGVH
HHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEE
IADVSHYVTEGSSLDEEAYSRATSVYVTDRVVPMLPVKLSNNLCSLNEAQERLTMSCLME
EEHHHHHHHCCCCCCHHHHHHHEEEEEECCCCEEECEECCCCCCCHHHHHHHHHHHHHHH
IDDKGKIVSYKISPSVIKTTYRMTYNNVNKMIHQGQEGHREALENFFKITDSIKVAVELH
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EILETMRKDRGMIEFDESEAKIILDEKGHPIEIVKRDRDTAERMIESFMLMANETVALDF
HHHHHHHHCCCCEEECCCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
QKKKLPSLYRVHDNPKEKSFAKLMEAAANAGFSLNSDSHQAINFFADEIKGTSSEKALTY
HHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
QLRHTMSTAVYSEKNTKHFGLAATNYTHFTSPIRRYPDLIIHRLLHLYPSDHSNHTKDEW
HHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KERLPEIASHSSDMEHRAVVTERIIDAMKKAEYMSERIGEVYTGTITGLQKFGIFVALDN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEECC
TVEGLIRVPNLHTGTTEELEFDEEASIFKGKKSETVYQIGQEIKIRVIAANKRKGTVDFE
CHHHHEECCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCEEEEEEEEECCCCCCCCHH
QIAPE
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 1400209