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The map label for this gene is folE

Identifier: 15673136

GI number: 15673136

Start: 1166187

End: 1167236

Strand: Direct

Name: folE

Synonym: L0175

Alternate gene names: 15673136

Gene position: 1166187-1167236 (Clockwise)

Preceding gene: 15673135

Following gene: 15673137

Centisome position: 49.3

GC content: 32.1

Gene sequence:

>1050_bases
ATGCAAACAACTTACTTAAGCATGGGAAGTAATATTGGTGACCGTCAGTATTATTTACATGAAGCCATTCGTTTATTGGG
AAAACACCCTAAAATTATGATTGAAAAAGTATCAAATTTTTATGAAAGTACTCCAGTCGGCGGCGTCAAACAAGATGATT
TTACTAATTTGGCATTAAAGGTGGCAACGCTACTTGAACCTTTGGAATTATTATCTTTTATTCATGAAGTTGAGTTATCT
TTGAACCGTGAGCGAAAAATTCATTGGGGGCCAAGAACAATTGATATTGATATTATTTTCTATGACGACTTAGAAATGCA
AGTAGAAAACTTGGTTATTCCACATAAAGAAGCTTTTAATCGTCTTTTTGTCTTGAAACCTATTTTTGAACTTATTGATA
AAGACTTTAAATATTATGCGTCAATAGAAAAAGCAATAGCCGAACTTTCAGTAAGTGAACAAGAGCTCCATGTAATAAAA
GAAGAAAAAACACCGAGAAATCGTATTGAAGATGCCGTTAAAGAGATTCTCTTTGCAGTAGGTGAAAATCCAAATCGAGA
AGGATTACTTGAAACTCCAGCAAGAGTAGCTAAAATGTATGAAGAAATTCTTTCGTCACAACGCTTAAGCAAGTTTAATG
AGTATAAACTTTTTGAAATTGATTCTTCTAAAACGGATTCAATCGTGTTGATTAAAGATATTCCTTTTTATTCAATGTGT
GAGCATCATATGTTACCATTTTTTGGGAAAGCTCATGTTGCATATATTCCAGCTGATGGAAAAATTATTGGCTTGTCAAA
AATTCCCCGTTTAGTTGATTATGTTTCGCGCAAACTCTCGGTTCAAGAAAATATCACTCATGATATTGGAGATATTTTGA
CTGATATTTTGAATCCTAAAGGAGTGGCAGTTCTTGTTGAAGGACGTCATATGTGCGTTGAAATGCGTGGAGTAAAAAAA
GTAAATTCTATTACTAAAACTTCTTATTTTTTAGGTGAATTTAAAGAAAATAATGAAAAAAGAATGGAATTTTTAGAAAG
TCTTTTATGA

Upstream 100 bases:

>100_bases
GAAATTGAGATGAGAGGCTAAAGCTGGTTTTTAAGATAAATATTTTAAAGAGATAGAAGAGAAACAAAATCATAAAAGAT
TATGTCTAAATGGAGGACTT

Downstream 100 bases:

>100_bases
AAATCTTAGAACTTAATCAAGAATCTTTTTCTCTTAAAAATATTATCCTAAAATTTGATGAGTTAAATCACAATGAAATG
ATTTCTCTTCAAAAAAAACT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase; 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8 dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK; GTP cyclohydrolase 1; GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESTPVGGVKQDDFTNLALKVATLLEPLELLSFIHEVELS
LNRERKIHWGPRTIDIDIIFYDDLEMQVENLVIPHKEAFNRLFVLKPIFELIDKDFKYYASIEKAIAELSVSEQELHVIK
EEKTPRNRIEDAVKEILFAVGENPNREGLLETPARVAKMYEEILSSQRLSKFNEYKLFEIDSSKTDSIVLIKDIPFYSMC
EHHMLPFFGKAHVAYIPADGKIIGLSKIPRLVDYVSRKLSVQENITHDIGDILTDILNPKGVAVLVEGRHMCVEMRGVKK
VNSITKTSYFLGEFKENNEKRMEFLESLL

Sequences:

>Translated_349_residues
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESTPVGGVKQDDFTNLALKVATLLEPLELLSFIHEVELS
LNRERKIHWGPRTIDIDIIFYDDLEMQVENLVIPHKEAFNRLFVLKPIFELIDKDFKYYASIEKAIAELSVSEQELHVIK
EEKTPRNRIEDAVKEILFAVGENPNREGLLETPARVAKMYEEILSSQRLSKFNEYKLFEIDSSKTDSIVLIKDIPFYSMC
EHHMLPFFGKAHVAYIPADGKIIGLSKIPRLVDYVSRKLSVQENITHDIGDILTDILNPKGVAVLVEGRHMCVEMRGVKK
VNSITKTSYFLGEFKENNEKRMEFLESLL
>Mature_349_residues
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESTPVGGVKQDDFTNLALKVATLLEPLELLSFIHEVELS
LNRERKIHWGPRTIDIDIIFYDDLEMQVENLVIPHKEAFNRLFVLKPIFELIDKDFKYYASIEKAIAELSVSEQELHVIK
EEKTPRNRIEDAVKEILFAVGENPNREGLLETPARVAKMYEEILSSQRLSKFNEYKLFEIDSSKTDSIVLIKDIPFYSMC
EHHMLPFFGKAHVAYIPADGKIIGLSKIPRLVDYVSRKLSVQENITHDIGDILTDILNPKGVAVLVEGRHMCVEMRGVKK
VNSITKTSYFLGEFKENNEKRMEFLESLL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=181, Percent_Identity=45.8563535911602, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503949, Length=181, Percent_Identity=45.8563535911602, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI66932970, Length=144, Percent_Identity=43.0555555555556, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI66932972, Length=144, Percent_Identity=43.0555555555556, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788476, Length=189, Percent_Identity=37.5661375661376, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786335, Length=132, Percent_Identity=37.8787878787879, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=42.9378531073446, Blast_Score=154, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321706, Length=185, Percent_Identity=45.4054054054054, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324073, Length=135, Percent_Identity=37.037037037037, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24656782, Length=172, Percent_Identity=46.5116279069767, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24656791, Length=172, Percent_Identity=46.5116279069767, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24656796, Length=172, Percent_Identity=46.5116279069767, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): FOLKE_LACLA (Q9CGE3)

Other databases:

- EMBL:   AE005176
- PIR:   B86769
- RefSeq:   NP_267310.1
- ProteinModelPortal:   Q9CGE3
- GeneID:   1114796
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0175
- NMPDR:   fig|272623.1.peg.1188
- HOGENOM:   HBG516998
- OMA:   VEMRGVK
- ProtClustDB:   CLSK876844
- BioCyc:   LLAC272623:L0175-MONOMER
- BRENDA:   2.7.6.3
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- InterPro:   IPR000550
- Gene3D:   G3DSA:3.30.70.560
- TIGRFAMs:   TIGR00063
- TIGRFAMs:   TIGR01498

Pfam domain/function: PF01227 GTP_cyclohydroI; PF01288 HPPK; SSF55083 Hppk

EC number: =2.7.6.3; =3.5.4.16

Molecular weight: Translated: 40268; Mature: 40268

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2; PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESTPVGGVKQDDFTNLALK
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VATLLEPLELLSFIHEVELSLNRERKIHWGPRTIDIDIIFYDDLEMQVENLVIPHKEAFN
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEEEEECCHHHHHHHCCCCCHHHHH
RLFVLKPIFELIDKDFKYYASIEKAIAELSVSEQELHVIKEEKTPRNRIEDAVKEILFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GENPNREGLLETPARVAKMYEEILSSQRLSKFNEYKLFEIDSSKTDSIVLIKDIPFYSMC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCHHHHH
EHHMLPFFGKAHVAYIPADGKIIGLSKIPRLVDYVSRKLSVQENITHDIGDILTDILNPK
HHCCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GVAVLVEGRHMCVEMRGVKKVNSITKTSYFLGEFKENNEKRMEFLESLL
CEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESTPVGGVKQDDFTNLALK
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VATLLEPLELLSFIHEVELSLNRERKIHWGPRTIDIDIIFYDDLEMQVENLVIPHKEAFN
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEEEEECCHHHHHHHCCCCCHHHHH
RLFVLKPIFELIDKDFKYYASIEKAIAELSVSEQELHVIKEEKTPRNRIEDAVKEILFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GENPNREGLLETPARVAKMYEEILSSQRLSKFNEYKLFEIDSSKTDSIVLIKDIPFYSMC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCHHHHH
EHHMLPFFGKAHVAYIPADGKIIGLSKIPRLVDYVSRKLSVQENITHDIGDILTDILNPK
HHCCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GVAVLVEGRHMCVEMRGVKKVNSITKTSYFLGEFKENNEKRMEFLESLL
CEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471