Click here to switch to the map view.

The map label for this gene is pcrA

Identifier: 15673102

GI number: 15673102

Start: 1133793

End: 1136069

Strand: Direct

Name: pcrA

Synonym: L0287

Alternate gene names: 15673102

Gene position: 1133793-1136069 (Clockwise)

Preceding gene: 15673100

Following gene: 15673103

Centisome position: 47.93

GC content: 36.8

Gene sequence:

>2277_bases
ATGAATCCACTATTACAAGGAATGAACGAAAAACAAGCAGAAGCGGTCCAAACTACAGAAGGGCCTTTATTGATTATGGC
AGGAGCTGGTTCGGGTAAGACTCGTGTCTTAACTCATCGAATTGCTTACTTAATTGATGAAAAAATGGTTAATCCTTGGA
ATATTTTAGCGATTACCTTTACAAATAAAGCAGCAAAAGAAATGCGGGAGCGTGCTTTATCACTTACTCCAAGAGCACAA
GATACATTAATTGCGACCTTTCACTCAATGTGTGTAAGAATTTTGAGACGTGATGCCGATCATATTGGTTATAATCGTAA
TTTTACAATTATAGATCCAGGGGACCAAAAATCGTTGATGAAACGAATTTTGAAAGAAGCTAATTTAGATCCTAAAAAAT
GGGAACCCAAAGGGCTTTTAAATGCGATTTCAAATGCTAAAAATGATTTATTAGATGAATCAGCTTATGAAGCTCAAACA
AGCGCTAGACATCCATATGAAATGGTTGTTGCGCGTGTTTATAAGATTTACCAAAAAGAATTACGAAAAGCAGAATCAAT
GGATTTTGACGATTTAATCATGCAGACGTTGCGCCTTTTTGATGAAAACCCGGATGTCCTTTCTTACTATCAAGGAAAAT
TTCAATATATTCATGTTGATGAGTATCAAGATACCAACCATGCACAATATCAGTTGGTTAAACTTTTAGCCAGTCGTTTT
CAAAATATCTGTGTCGTTGGGGATGCTGACCAATCAATCTATGGTTGGCGCGGAGCAGATATGCAAAATATCTTAGACTT
TGAAAAAGATTATCCAAAAGCTAAAGTTGTTTTGCTTGAAGAAAATTACCGCTCAACAAAAACAATTCTTGATGCGGCAA
ACAATGTTATAAAAAATAATTTAAAACGTCGACCAAAAGAACTCTGGACGCAAAATGATGCTGGAGATAAGATTGTTTAT
TTCCGCGCTGGAAATGAACAAGAAGAAGCAAATAAGGTTTCTGAAATTATCACATCAGGCATTCGTTCAGGACGTTCTTA
TGCTGACTATGCTGTGCTTTATCGGACTAATGCTCAGTCTCGGACAATTGAAGATTCTTTTGTTAAATCTAATATTCCCT
ATACAATGGTTGCGGGAACTAAATTCTACTCACGTAAGGAAATTCGTGATGTTATTGCTTACTTAAATGTAGTAGCAAAT
CCAGCGGATAATATGAGTTTTGAACGAATTGTTAATGAACCTAAACGAGGAGTCGGTCCGGGGGCATTAGAAAAATTACG
TCGTTTTGCTGACAGTCGTGGACTTTCTTTAGCAGATTCAAGTTTTGATATTATGTTATCCGATATTCGTGGAAAAGCGG
CTGGTGAGATTTATAATTTGGCAATGGCCTTTGATTTGTTGAGAACCCATAGTGAAAGTTCAACAATCACAGAATTAGTT
GAAGAAATGTTAGAGCGTACTGGCTATGTCAAAGCTTTGCAAATGATGCCAAGTATCGAAAATCAAGCACGAATTGAAAA
TATTGAAGAATTCTTGTCAGTAACTAAAGGGTTTGATGAAAAATCAGAAATTCCTCTAGATGAAGAAACGGGAGAGCCAA
TTGTTGAAACTGGATTAGATAAGTTGAGCCGTTTCCTTAACGAAGTTAGTTTGCTTTCTGAAACAGATGCTTATGAGGAA
GAATCTGACCAAGTAACAATGATGACCCTGCATGCGGCAAAAGGTTTGGAATTTCCAATTGTTTTCTTAATTGGAATGGA
AGAAAACATTTTTCCTTTATCACGGGTAAATGAAGATATTGATGAGTTGGAAGAAGAACGTCGTTTGGCTTATGTTGGAA
TTACACGGGCAGAAGAAACGCTCTACTTAATGAATGCCAATCAACGTGTGCTTTATGGTAAAACGAATTATAATCGTCCC
AGCCGTTTTATTTCGGAAATTGATGATGAATTACTAGAATATGCTGGGATTGCTCGTAAAGCAAATTCAAGCTTCAATGC
TTCTTATAAATCTGGTGGTTTTGCCAGTGGGATCTCGATGTCAGATGCGCTTCATCAAAGAAAAGCAGTCATTAATCCAA
CTGCAGCTCAAGCGACGATTAATAATTCTGAAACAGAAGATTGGCAAATTGGCGATACAGCGATTCACCGTAAATGGGGC
GAAGGTATGGTATTAGGAATTTCTGGTTCTGGTAAAAATATGGAACTCAAGATTAATTTCCCAGAAGTCGGAATGAAACG
TTTATTAGCAGCAATGGCTCCAATTGAGAAAAAATAG

Upstream 100 bases:

>100_bases
TTATTAGGCTTAGACCATAAAAATAGAAGATTATAAGAGGCTCGCATTAAGTGATTTACTTATGATATAATTAATAATGA
TTTGCAGCTAGGAGAATAAA

Downstream 100 bases:

>100_bases
AAAAAGTAGTGAAAAAATAATGACTTATCATGAGTTAGAACAAGTCGAATTGACAAATATGTGTGCCATTATTGACGAAA
AAACCCATAAAGTTCTTGTT

Product: ATP-dependent helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MNPLLQGMNEKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITFTNKAAKEMRERALSLTPRAQ
DTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLMKRILKEANLDPKKWEPKGLLNAISNAKNDLLDESAYEAQT
SARHPYEMVVARVYKIYQKELRKAESMDFDDLIMQTLRLFDENPDVLSYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNNLKRRPKELWTQNDAGDKIVY
FRAGNEQEEANKVSEIITSGIRSGRSYADYAVLYRTNAQSRTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVAN
PADNMSFERIVNEPKRGVGPGALEKLRRFADSRGLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
EEMLERTGYVKALQMMPSIENQARIENIEEFLSVTKGFDEKSEIPLDEETGEPIVETGLDKLSRFLNEVSLLSETDAYEE
ESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDIDELEEERRLAYVGITRAEETLYLMNANQRVLYGKTNYNRP
SRFISEIDDELLEYAGIARKANSSFNASYKSGGFASGISMSDALHQRKAVINPTAAQATINNSETEDWQIGDTAIHRKWG
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK

Sequences:

>Translated_758_residues
MNPLLQGMNEKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITFTNKAAKEMRERALSLTPRAQ
DTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLMKRILKEANLDPKKWEPKGLLNAISNAKNDLLDESAYEAQT
SARHPYEMVVARVYKIYQKELRKAESMDFDDLIMQTLRLFDENPDVLSYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNNLKRRPKELWTQNDAGDKIVY
FRAGNEQEEANKVSEIITSGIRSGRSYADYAVLYRTNAQSRTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVAN
PADNMSFERIVNEPKRGVGPGALEKLRRFADSRGLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
EEMLERTGYVKALQMMPSIENQARIENIEEFLSVTKGFDEKSEIPLDEETGEPIVETGLDKLSRFLNEVSLLSETDAYEE
ESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDIDELEEERRLAYVGITRAEETLYLMNANQRVLYGKTNYNRP
SRFISEIDDELLEYAGIARKANSSFNASYKSGGFASGISMSDALHQRKAVINPTAAQATINNSETEDWQIGDTAIHRKWG
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK
>Mature_758_residues
MNPLLQGMNEKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITFTNKAAKEMRERALSLTPRAQ
DTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLMKRILKEANLDPKKWEPKGLLNAISNAKNDLLDESAYEAQT
SARHPYEMVVARVYKIYQKELRKAESMDFDDLIMQTLRLFDENPDVLSYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNNLKRRPKELWTQNDAGDKIVY
FRAGNEQEEANKVSEIITSGIRSGRSYADYAVLYRTNAQSRTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVAN
PADNMSFERIVNEPKRGVGPGALEKLRRFADSRGLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
EEMLERTGYVKALQMMPSIENQARIENIEEFLSVTKGFDEKSEIPLDEETGEPIVETGLDKLSRFLNEVSLLSETDAYEE
ESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDIDELEEERRLAYVGITRAEETLYLMNANQRVLYGKTNYNRP
SRFISEIDDELLEYAGIARKANSSFNASYKSGGFASGISMSDALHQRKAVINPTAAQATINNSETEDWQIGDTAIHRKWG
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=757, Percent_Identity=38.0449141347424, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI48994965, Length=654, Percent_Identity=39.9082568807339, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787196, Length=332, Percent_Identity=25.9036144578313, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322369, Length=735, Percent_Identity=30.4761904761905, Blast_Score=237, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324477, Length=675, Percent_Identity=23.2592592592593, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85834; Mature: 85834

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLQGMNEKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITF
CCHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAKEMRERALSLTPRAQDTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLM
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEECCCCHHHHH
KRILKEANLDPKKWEPKGLLNAISNAKNDLLDESAYEAQTSARHPYEMVVARVYKIYQKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LRKAESMDFDDLIMQTLRLFDENPDVLSYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHH
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNN
CCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LKRRPKELWTQNDAGDKIVYFRAGNEQEEANKVSEIITSGIRSGRSYADYAVLYRTNAQS
HHHCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVANPADNMSFERIVNEPKRGVGP
CCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCH
GALEKLRRFADSRGLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
HHHHHHHHHHHHCCCEECCCCHHHHEEHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EEMLERTGYVKALQMMPSIENQARIENIEEFLSVTKGFDEKSEIPLDEETGEPIVETGLD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
KLSRFLNEVSLLSETDAYEEESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDI
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCHHHHCHHH
DELEEERRLAYVGITRAEETLYLMNANQRVLYGKTNYNRPSRFISEIDDELLEYAGIARK
HHHHHHCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ANSSFNASYKSGGFASGISMSDALHQRKAVINPTAAQATINNSETEDWQIGDTAIHRKWG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHCC
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK
CEEEEEEECCCCCEEEEEECCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNPLLQGMNEKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITF
CCHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAKEMRERALSLTPRAQDTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLM
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEECCCCHHHHH
KRILKEANLDPKKWEPKGLLNAISNAKNDLLDESAYEAQTSARHPYEMVVARVYKIYQKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LRKAESMDFDDLIMQTLRLFDENPDVLSYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHH
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNN
CCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LKRRPKELWTQNDAGDKIVYFRAGNEQEEANKVSEIITSGIRSGRSYADYAVLYRTNAQS
HHHCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVANPADNMSFERIVNEPKRGVGP
CCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCH
GALEKLRRFADSRGLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
HHHHHHHHHHHHCCCEECCCCHHHHEEHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EEMLERTGYVKALQMMPSIENQARIENIEEFLSVTKGFDEKSEIPLDEETGEPIVETGLD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
KLSRFLNEVSLLSETDAYEEESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDI
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCHHHHCHHH
DELEEERRLAYVGITRAEETLYLMNANQRVLYGKTNYNRPSRFISEIDDELLEYAGIARK
HHHHHHCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ANSSFNASYKSGGFASGISMSDALHQRKAVINPTAAQATINNSETEDWQIGDTAIHRKWG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHCC
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK
CEEEEEEECCCCCEEEEEECCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA