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The map label for this gene is nadE

Identifier: 15673084

GI number: 15673084

Start: 1109757

End: 1110581

Strand: Direct

Name: nadE

Synonym: L0203

Alternate gene names: 15673084

Gene position: 1109757-1110581 (Clockwise)

Preceding gene: 15673083

Following gene: 15673085

Centisome position: 46.91

GC content: 39.15

Gene sequence:

>825_bases
ATGACATTACAAGATGAAATTATTAAAGAACTTGGTGTGAAACCAGTCATTGATCCTAAAGAAGAAATTAGGGTGTCAGT
TGATTTTTTGAAAGATTATCTAAAAAAATATCCTTTCATTAAGTCTTTTGTATTAGGGATTTCTGGTGGTCAAGATTCTA
GTTTAGCAGGTCGTCTGGCTCAAATTGCTATCGAAGAAATGCGTCAAGAAACTGCTGATGAGACTTATAAATTTGTAGCA
ATTCGCCTGCCATATGGTGTTCAAGCTGATGAAGAAGATGCCCAACGTGCGCTTGCTTTCATTCAACCCGATGTCAGTTT
GACAGTAAATATTAAAGCTGCTGTTGAAGGACAAGTGGCAGCCCTCAATGAAGCTGGAATTGAAGTATCAGATTTCAACA
AAGGAAATATTAAGGCACGGCAACGTATGATTACGCAATATGCTGTGGCTGGTCAATATCAAGGAGCTGTTCTTGGAACA
GACCATGCAGCAGAAAATATTACTGGATTTTTCACAAAATTTGGTGATGGTGGAGCAGACTTGCTCCCACTTTTCCGCTT
AAATAAACGCCAAGGAAAAGCACTCTTGGCTGAACTTGGCGCTGACCCAGCCATTTATGAGAAAGTTCCGACTGCAGACC
TTGAAGAAGGAAAACCAGGACTTGCTGACGAGATTGCATTAGGTGTGACTTACAATGATATTGATGACTACACAGAAGGT
AAAGTGATTTCTGAGGATGCTAAGGCTAAAATCGAAGCATGGTGGAAGAAAACACAACATAAACGTCACTTACCAATTAG
TGTTTTTGATGATTTTTGGAAATAA

Upstream 100 bases:

>100_bases
ATACATTGATGAATTCTATATGAGCAAAATTTTAGATTAAAAATAGTTAATGACAGAGCTGTCAGTAAAATATAGAAACT
GTCAGTAAAAGGAAGATAAA

Downstream 100 bases:

>100_bases
ACAAAAAATTACTGACAGGATTTCTGTCAGTAATTTTTTTATTAAAAATGATGAGGACTTGAATAAGAAGTAAAATGTAA
TTATGATATAATTAACTAAA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLQDEIIKELGVKPVIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLAQIAIEEMRQETADETYKFVA
IRLPYGVQADEEDAQRALAFIQPDVSLTVNIKAAVEGQVAALNEAGIEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGT
DHAAENITGFFTKFGDGGADLLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
KVISEDAKAKIEAWWKKTQHKRHLPISVFDDFWK

Sequences:

>Translated_274_residues
MTLQDEIIKELGVKPVIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLAQIAIEEMRQETADETYKFVA
IRLPYGVQADEEDAQRALAFIQPDVSLTVNIKAAVEGQVAALNEAGIEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGT
DHAAENITGFFTKFGDGGADLLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
KVISEDAKAKIEAWWKKTQHKRHLPISVFDDFWK
>Mature_273_residues
TLQDEIIKELGVKPVIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLAQIAIEEMRQETADETYKFVAI
RLPYGVQADEEDAQRALAFIQPDVSLTVNIKAAVEGQVAALNEAGIEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGTD
HAAENITGFFTKFGDGGADLLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEGK
VISEDAKAKIEAWWKKTQHKRHLPISVFDDFWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=65.3284671532847, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_LACLA (Q9CGJ4)

Other databases:

- EMBL:   AE005176
- PIR:   F86762
- RefSeq:   NP_267258.1
- ProteinModelPortal:   Q9CGJ4
- SMR:   Q9CGJ4
- GeneID:   1114742
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0203
- NMPDR:   fig|272623.1.peg.1134
- HOGENOM:   HBG351567
- OMA:   GLNKRRV
- ProtClustDB:   PRK00768
- BioCyc:   LLAC272623:L0203-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30282; Mature: 30150

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQDEIIKELGVKPVIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLA
CCCHHHHHHHCCCCCCCCCHHHHEEEHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHH
QIAIEEMRQETADETYKFVAIRLPYGVQADEEDAQRALAFIQPDVSLTVNIKAAVEGQVA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCEE
ALNEAGIEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGTDHAAENITGFFTKFGDGGAD
EHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
LLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
HHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCC
KVISEDAKAKIEAWWKKTQHKRHLPISVFDDFWK
CEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TLQDEIIKELGVKPVIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLA
CCHHHHHHHCCCCCCCCCHHHHEEEHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHH
QIAIEEMRQETADETYKFVAIRLPYGVQADEEDAQRALAFIQPDVSLTVNIKAAVEGQVA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCEE
ALNEAGIEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGTDHAAENITGFFTKFGDGGAD
EHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
LLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
HHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCC
KVISEDAKAKIEAWWKKTQHKRHLPISVFDDFWK
CEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471