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The map label for this gene is ribH

Identifier: 15672978

GI number: 15672978

Start: 1024999

End: 1025463

Strand: Direct

Name: ribH

Synonym: L0166

Alternate gene names: 15672978

Gene position: 1024999-1025463 (Clockwise)

Preceding gene: 15672977

Following gene: 15672979

Centisome position: 43.33

GC content: 35.27

Gene sequence:

>465_bases
ATGACAATTATTGAAGGAAATTTAAGAACAGAGAATGCAAAATATGGGATTGTAGTTGCTCAATTTAATGAATTTATCAA
TGCAAAACTTTTGTCAGGAGCATTAGATGCACTCAAAAGACATGGAGTCCAGGAAGAAGAAATTGATATAGCATGGGTTC
CAGGTGCATTTGAAATTCCCATTATTGCCCAAAAAATGGCGACTTCCAATAAATATGAAGCAATTATATGTTTAGGGACT
GTCATCAGAGGCTCAACTTCTCATTATGATTTTGTTTGTAATGAAGTTTCTAAGGGGATTGCTCATGTTTCCTTAAACTC
TAATATTCCGGTCATGTTTGGTGTACTAACAACTGAAAATATAGAACAAGCCATTGAGCGTGCAGGCACAAAAGCTGGAA
ATAAAGGTTTTGAAGTAGCGGTTGGAGCAATTGAAATGGTTGATTTAATGAGAAAGATTGGCTAA

Upstream 100 bases:

>100_bases
AATCAAAGATAACCCCTTTGACCTTGATTATTTAAAAGTAAAACAAAATAAAATGGGACATTTGTTTGACTAAAGTAAAA
TAGAAAGTAAGGATAGAAAT

Downstream 100 bases:

>100_bases
TTTATCTATAAGTGCATTTATTTAAATGCGCTTTTTATTTTGAAATAGGATAAAAACTGTTCTAGCGAGCTATCAGATTG
CGAAATATGCTATAATTAAA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTIIEGNLRTENAKYGIVVAQFNEFINAKLLSGALDALKRHGVQEEEIDIAWVPGAFEIPIIAQKMATSNKYEAIICLGT
VIRGSTSHYDFVCNEVSKGIAHVSLNSNIPVMFGVLTTENIEQAIERAGTKAGNKGFEVAVGAIEMVDLMRKIG

Sequences:

>Translated_154_residues
MTIIEGNLRTENAKYGIVVAQFNEFINAKLLSGALDALKRHGVQEEEIDIAWVPGAFEIPIIAQKMATSNKYEAIICLGT
VIRGSTSHYDFVCNEVSKGIAHVSLNSNIPVMFGVLTTENIEQAIERAGTKAGNKGFEVAVGAIEMVDLMRKIG
>Mature_153_residues
TIIEGNLRTENAKYGIVVAQFNEFINAKLLSGALDALKRHGVQEEEIDIAWVPGAFEIPIIAQKMATSNKYEAIICLGTV
IRGSTSHYDFVCNEVSKGIAHVSLNSNIPVMFGVLTTENIEQAIERAGTKAGNKGFEVAVGAIEMVDLMRKIG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=57.1428571428571, Blast_Score=186, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6324429, Length=142, Percent_Identity=29.5774647887324, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_LACLA (Q9CGU6)

Other databases:

- EMBL:   AE005176
- PIR:   D86749
- RefSeq:   NP_267152.1
- ProteinModelPortal:   Q9CGU6
- SMR:   Q9CGU6
- GeneID:   1114627
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0166
- NMPDR:   fig|272623.1.peg.1019
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   LLAC272623:L0166-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16697; Mature: 16565

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIEGNLRTENAKYGIVVAQFNEFINAKLLSGALDALKRHGVQEEEIDIAWVPGAFEIP
CEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECC
IIAQKMATSNKYEAIICLGTVIRGSTSHYDFVCNEVSKGIAHVSLNSNIPVMFGVLTTEN
HHHHHHHCCCCCEEEEEEEEHHCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEECHHH
IEQAIERAGTKAGNKGFEVAVGAIEMVDLMRKIG
HHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIIEGNLRTENAKYGIVVAQFNEFINAKLLSGALDALKRHGVQEEEIDIAWVPGAFEIP
EEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECC
IIAQKMATSNKYEAIICLGTVIRGSTSHYDFVCNEVSKGIAHVSLNSNIPVMFGVLTTEN
HHHHHHHCCCCCEEEEEEEEHHCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEECHHH
IEQAIERAGTKAGNKGFEVAVGAIEMVDLMRKIG
HHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471