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The map label for this gene is gyrB

Identifier: 15672887

GI number: 15672887

Start: 927935

End: 929890

Strand: Direct

Name: gyrB

Synonym: L0283

Alternate gene names: 15672887

Gene position: 927935-929890 (Clockwise)

Preceding gene: 15672886

Following gene: 15672888

Centisome position: 39.23

GC content: 38.34

Gene sequence:

>1956_bases
TTGAACGAAGAAAATAAAAATTTAGAAATGCTTGCAGACGAATATGACGCCAGTCAAATTCAAGTACTTGAAGGTCTTGA
AGCAGTACGTATGCGTCCAGGGATGTATATTGGTTCAACCTCAAAAGAAGGGTTGCACCATTTAGTCTGGGAAATTGTTG
ACAATTCAATTGATGAAGCACTTGCAGGATTTGCAAGCCATATTGAGGTTTTTATTGAGCCTGATAATTCAATTACTGTT
GTTGATGATGGACGTGGAATTCCGGTTGATATTCAAGAAAAAACGGGTCGTCCAGCCGTGGAAACAGTCTTCACAGTGTT
GCATGCTGGAGGTAAATTTGGTGGAGGCGGCTATAAAGTTTCTGGTGGATTGCACGGTGTTGGTTCATCTGTTGTTAATG
CTTTGTCAACAGTACTAGATGTTACTGTTCATAAAGATGGCCAAAAATATTATCAAGAATATCATCGTGGTCTCGTTGGA
GATGATTTAAAAATTATTGGTGAAACTGACCGTAGAGGGACAACTGTTCACTTTGTACCAGACCCAGAAATTTTTACAGA
AACTGTTGAATTTGATTTTGATAAGCTTGCAACACGTGTTCGTGAGTTAGCCTTTTTGAATCGCGGTCTTCGCATTTCCT
TAACAGACAAACGTGAAGGAAATGAAAAAAATGAAGTTCACTTCCATTATGAAGGTGGAATTCAGTCTTATGTTGAATAT
TTAAATGAAAATAAGACAGTCATTTTTGAACCAGCAATTTTTACTGAAGGTGAAATGGATGGAATTGCTGTTGAAGTAGC
AATGCAATATACTGATACTTATCATTCAGTAGTGATGTCCTTTGCTAATAACATCAACACTCATGAAGGTGGAACACATG
AGCAAGGTTTTAGAACAGCCCTTACTCGTGTTATTAATAATTACGCAAAACAAAATAAAATTCTTAAAGAAAACGAAGAT
AATTTAACTGGGGATGATGTCCGCGAAGGATTGACAGCAGTTATTTCGGTTAAGCATCCTAATCCACAATTTGAAGGGCA
AACCAAAACAAAACTTGGGAACTCAGAAGTAACAGGAATTGTTAACAAACTCTTTGCGGAAGCTTTGACAACATTTATGT
TAGAAAATCCACAAGTTGCTCGTAAAATCGTTGAAAAAGGGATTTTGGCAAGTAAAGCAAGGATTGCAGCAAAACGTGCG
CGTGAAGTGACACGTAAAAAATCAGGGCTTGAAATTTCCAACCTTCCTGGTAAATTAGCTGACTGTTCATCAAATAATCC
AGAACAAACAGAATTATTCATCGTCGAAGGAGATTCGGCTGGAGGTTCGGCTAAATCAGGACGTAATCGTGAATTCCAAG
CAATTTTACCTATTCGTGGTAAGATTTTGAATGTGGAAAAAGCAACGATGGATAAAATTTTGGCCAATGAAGAAATTCGT
TCTCTATTCACTGCAATGGGAACTGGTTTTGGAGCAGATTATGATTTGTCGAAAGCACGTTATCACAAACTTGTTATCAT
GACCGATGCCGATGTCGACGGAGCACACATCCGTACGCTTTTACTAACACTCTTCTACCGTTATATGCGCCCAGTTGTTG
AAGCAGGGTATGTTTATATTGCTCAGCCACCAATTTACGGTATCAAAGTTGGTTCAGAAACAAAAGAATACATTCAACCA
GGTGAAAATCAAGAAAAAGATTTACAAGAAGCTTTAGCACGTTGGGCACAAGGAAGAACAAAGCCTACTGTACAACGTTA
TAAAGGTTTAGGGGAAATGGATGACCATCAACTTTGGGATACAACAATGGATCCTGAACACCGTTTAATGGCACGTGTAT
CTGTTGAAGACGCAGCAGAAGCTGACAAAATCTTTGATATGCTCATGGGAGACAGAGTTGAACCTCGTCGTGAATTTATT
GAAAATAATGCTCAGTACTCGACAATTGATGCATAA

Upstream 100 bases:

>100_bases
TTTTTTACAAGTTTGAGTGACAAAATACCATGCACTAGAGCTAAAATCGCTGATTTATGGTATAATAGTAGTAAGTTTTT
CATAGAAATGAGAGTTAACT

Downstream 100 bases:

>100_bases
TATAGTAAAAACAACTCATTTTTGTTTGAGTTGTTTTTTTATTGAACATAATGTTAGCGCTTTATCGAAAAGTGTAATAT
AATGAAGTCAAGAAGGAGGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEPDNSITV
VDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTVLDVTVHKDGQKYYQEYHRGLVG
DDLKIIGETDRRGTTVHFVPDPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKILKENED
NLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRA
REVTRKKSGLEISNLPGKLADCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
SLFTAMGTGFGADYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSETKEYIQP
GENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWDTTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFI
ENNAQYSTIDA

Sequences:

>Translated_651_residues
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEPDNSITV
VDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTVLDVTVHKDGQKYYQEYHRGLVG
DDLKIIGETDRRGTTVHFVPDPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKILKENED
NLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRA
REVTRKKSGLEISNLPGKLADCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
SLFTAMGTGFGADYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSETKEYIQP
GENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWDTTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFI
ENNAQYSTIDA
>Mature_651_residues
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEPDNSITV
VDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTVLDVTVHKDGQKYYQEYHRGLVG
DDLKIIGETDRRGTTVHFVPDPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKILKENED
NLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRA
REVTRKKSGLEISNLPGKLADCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
SLFTAMGTGFGADYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSETKEYIQP
GENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWDTTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFI
ENNAQYSTIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=690, Percent_Identity=25.6521739130435, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI19913406, Length=679, Percent_Identity=25.6259204712813, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=53.9426523297491, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789408, Length=645, Percent_Identity=40.3100775193798, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI212645845, Length=604, Percent_Identity=24.5033112582781, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17535065, Length=677, Percent_Identity=24.6676514032496, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=229, Percent_Identity=25.764192139738, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=645, Percent_Identity=26.046511627907, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=688, Percent_Identity=25.7267441860465, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72551; Mature: 72551

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEA
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LAGFASHIEVFIEPDNSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKV
HHHHHHCEEEEEECCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTVLDVTVHKDGQKYYQEYHRGLVGDDLKIIGETDRRGTTVHFVP
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECC
DPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHH
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTA
HCCCCEEEECCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LTRVINNYAKQNKILKENEDNLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHH
VNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
DCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHH
SLFTAMGTGFGADYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYI
HHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPIYGIKVGSETKEYIQPGENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWD
ECCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCC
TTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFIENNAQYSTIDA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEA
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LAGFASHIEVFIEPDNSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKV
HHHHHHCEEEEEECCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTVLDVTVHKDGQKYYQEYHRGLVGDDLKIIGETDRRGTTVHFVP
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECC
DPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHH
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTA
HCCCCEEEECCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LTRVINNYAKQNKILKENEDNLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHH
VNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
DCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHH
SLFTAMGTGFGADYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYI
HHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPIYGIKVGSETKEYIQPGENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWD
ECCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCC
TTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFIENNAQYSTIDA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7608096; 11463916 [H]