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The map label for this gene is yijG

Identifier: 15672858

GI number: 15672858

Start: 898813

End: 900141

Strand: Direct

Name: yijG

Synonym: L98542

Alternate gene names: 15672858

Gene position: 898813-900141 (Clockwise)

Preceding gene: 15672857

Following gene: 15672859

Centisome position: 38.0

GC content: 33.94

Gene sequence:

>1329_bases
ATGATTAGTTTTGTTCAAACGGGAAATGGCATTGTTGACTTTATTCTGACACTGATATTTCTGGTGTTAATTACTTACCC
AATTTTAGGCGGATTCGCCTGGTTTATTGGAGTCTTATGTTATAGCTTTTTGTTCAAATATAAGCAAAAGAATTGGGATG
ATATTCCAGTAGAAGTTGAACCTTTTGTTACTATAATGGTTCCAGCTCATAATGAAGAAATAGTCATTGAAGATACACTT
AATTATTTAATGACAAAAATTAATTATGAAAATTATGAGGTTCTAGTTACTGATGATGGTTCAACTGATAGAACTCCCGA
AATTTTAAAAGAATTACAAGAAAAATATGATAAATTGCGCGTGATTAGAATTGAAAAAAATAAAGGAAAAGCGCATGCCT
TTAATATTGGTTTAGGATTTGCAAAAGGAGAACTAGTTTTAAGCAACGATGCAGATACTGTTCCAGAACCTGACTCTTTA
AATCGCTATATCAATTATTTTATTCGACAAGATTCTACTAACATCTCAGCAGTAACAGCTAATATGGATGTTCAGAATCG
TGCAAAATTGATTGCGAAATCTCAAACAGTTGAATTTTCTAGTATTGTAGGAATTATTAAACGGACTCAACTTGGTGTGC
TAGGGAGTATTTATGCCTATAGTGGTGCAAATACGATGTATCGAAAAGATGCCTTAATTGATGTCGGTTTGTTTCGACAA
GATAGAGCCACAGAAGATATTAGTATTGCTTGGGACCATCAAATGGACGGTTGGTTATCATTGTTTGCGCCTCGTATTAT
GTTTTTTATGGAGGTTCCAGAATCTCTGAAAATGCTCTATCGTCAAAGGAAACGGTGGGCCAAAGGTGGAACAGAGGTTT
GGCTGACTAATTTTAAAAAAGTATTTTTACACCCTTTTGACAACGTTGGCCGAACAATTATGTTTATTGACCAAAGTTTC
AGCATTATCTGGTCATTCTTTTTCTGGATTTCGTCAGTAATTTTTATCTGGTCTTTATTTCAATTTGCCATTACCGGAAA
CTTAGAACGGATTTATCACATGTTAACGATGGCCTTTCTCTTTGTTTGCTTTGAAATGATTTCGGGAACTTTTCAATTAG
TCGCTTCATTAATAGTTGATGACCGTGGAAGAAAAGTAAAATATCTATTATTTGCTCCACTTTATATGCTTTTTTACTGG
GTAATGAATGCATTAACCATTGTAACGACTTTTATTCCAGCAGTAAAAACTATTCTAGGTTTAGGGTCTGGAACCTGGAA
AAGTCCGGAGCGTAAAGGGATAAAAGTGACAAATAAAAAAGATAATTGA

Upstream 100 bases:

>100_bases
ACAAAAGATTTTCGCCATAATGTTTGTTTTTATTCGGTCAAAGAAGAGCAAAATTTGGAAACAGATTTTGTGAAAAAACT
TTATCAGAAGGGAGAAAACA

Downstream 100 bases:

>100_bases
AATTACTAACGATAAAAGTTTGATTTTATATCTTATCATTTGAAAATTTTTGAGGAGTTAAAGTACTCCTTTTTTTGAAT
TAACTTTTTTTAAGAAGACT

Product: glycosyl transferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MISFVQTGNGIVDFILTLIFLVLITYPILGGFAWFIGVLCYSFLFKYKQKNWDDIPVEVEPFVTIMVPAHNEEIVIEDTL
NYLMTKINYENYEVLVTDDGSTDRTPEILKELQEKYDKLRVIRIEKNKGKAHAFNIGLGFAKGELVLSNDADTVPEPDSL
NRYINYFIRQDSTNISAVTANMDVQNRAKLIAKSQTVEFSSIVGIIKRTQLGVLGSIYAYSGANTMYRKDALIDVGLFRQ
DRATEDISIAWDHQMDGWLSLFAPRIMFFMEVPESLKMLYRQRKRWAKGGTEVWLTNFKKVFLHPFDNVGRTIMFIDQSF
SIIWSFFFWISSVIFIWSLFQFAITGNLERIYHMLTMAFLFVCFEMISGTFQLVASLIVDDRGRKVKYLLFAPLYMLFYW
VMNALTIVTTFIPAVKTILGLGSGTWKSPERKGIKVTNKKDN

Sequences:

>Translated_442_residues
MISFVQTGNGIVDFILTLIFLVLITYPILGGFAWFIGVLCYSFLFKYKQKNWDDIPVEVEPFVTIMVPAHNEEIVIEDTL
NYLMTKINYENYEVLVTDDGSTDRTPEILKELQEKYDKLRVIRIEKNKGKAHAFNIGLGFAKGELVLSNDADTVPEPDSL
NRYINYFIRQDSTNISAVTANMDVQNRAKLIAKSQTVEFSSIVGIIKRTQLGVLGSIYAYSGANTMYRKDALIDVGLFRQ
DRATEDISIAWDHQMDGWLSLFAPRIMFFMEVPESLKMLYRQRKRWAKGGTEVWLTNFKKVFLHPFDNVGRTIMFIDQSF
SIIWSFFFWISSVIFIWSLFQFAITGNLERIYHMLTMAFLFVCFEMISGTFQLVASLIVDDRGRKVKYLLFAPLYMLFYW
VMNALTIVTTFIPAVKTILGLGSGTWKSPERKGIKVTNKKDN
>Mature_442_residues
MISFVQTGNGIVDFILTLIFLVLITYPILGGFAWFIGVLCYSFLFKYKQKNWDDIPVEVEPFVTIMVPAHNEEIVIEDTL
NYLMTKINYENYEVLVTDDGSTDRTPEILKELQEKYDKLRVIRIEKNKGKAHAFNIGLGFAKGELVLSNDADTVPEPDSL
NRYINYFIRQDSTNISAVTANMDVQNRAKLIAKSQTVEFSSIVGIIKRTQLGVLGSIYAYSGANTMYRKDALIDVGLFRQ
DRATEDISIAWDHQMDGWLSLFAPRIMFFMEVPESLKMLYRQRKRWAKGGTEVWLTNFKKVFLHPFDNVGRTIMFIDQSF
SIIWSFFFWISSVIFIWSLFQFAITGNLERIYHMLTMAFLFVCFEMISGTFQLVASLIVDDRGRKVKYLLFAPLYMLFYW
VMNALTIVTTFIPAVKTILGLGSGTWKSPERKGIKVTNKKDN

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Homo sapiens, GI7019323, Length=115, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787259, Length=414, Percent_Identity=28.9855072463768, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1786560, Length=219, Percent_Identity=27.8538812785388, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25146207, Length=109, Percent_Identity=33.9449541284404, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 50962; Mature: 50962

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFVQTGNGIVDFILTLIFLVLITYPILGGFAWFIGVLCYSFLFKYKQKNWDDIPVEVE
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
PFVTIMVPAHNEEIVIEDTLNYLMTKINYENYEVLVTDDGSTDRTPEILKELQEKYDKLR
CEEEEEEECCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCEE
VIRIEKNKGKAHAFNIGLGFAKGELVLSNDADTVPEPDSLNRYINYFIRQDSTNISAVTA
EEEEECCCCCEEEEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
NMDVQNRAKLIAKSQTVEFSSIVGIIKRTQLGVLGSIYAYSGANTMYRKDALIDVGLFRQ
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCC
DRATEDISIAWDHQMDGWLSLFAPRIMFFMEVPESLKMLYRQRKRWAKGGTEVWLTNFKK
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHH
VFLHPFDNVGRTIMFIDQSFSIIWSFFFWISSVIFIWSLFQFAITGNLERIYHMLTMAFL
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVCFEMISGTFQLVASLIVDDRGRKVKYLLFAPLYMLFYWVMNALTIVTTFIPAVKTILG
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSGTWKSPERKGIKVTNKKDN
CCCCCCCCCCCCCCEECCCCCC
>Mature Secondary Structure
MISFVQTGNGIVDFILTLIFLVLITYPILGGFAWFIGVLCYSFLFKYKQKNWDDIPVEVE
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
PFVTIMVPAHNEEIVIEDTLNYLMTKINYENYEVLVTDDGSTDRTPEILKELQEKYDKLR
CEEEEEEECCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCEE
VIRIEKNKGKAHAFNIGLGFAKGELVLSNDADTVPEPDSLNRYINYFIRQDSTNISAVTA
EEEEECCCCCEEEEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
NMDVQNRAKLIAKSQTVEFSSIVGIIKRTQLGVLGSIYAYSGANTMYRKDALIDVGLFRQ
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCC
DRATEDISIAWDHQMDGWLSLFAPRIMFFMEVPESLKMLYRQRKRWAKGGTEVWLTNFKK
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHH
VFLHPFDNVGRTIMFIDQSFSIIWSFFFWISSVIFIWSLFQFAITGNLERIYHMLTMAFL
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVCFEMISGTFQLVASLIVDDRGRKVKYLLFAPLYMLFYWVMNALTIVTTFIPAVKTILG
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSGTWKSPERKGIKVTNKKDN
CCCCCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]