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The map label for this gene is recN

Identifier: 15672842

GI number: 15672842

Start: 883095

End: 884762

Strand: Direct

Name: recN

Synonym: L0264

Alternate gene names: 15672842

Gene position: 883095-884762 (Clockwise)

Preceding gene: 15672841

Following gene: 15672843

Centisome position: 37.33

GC content: 34.59

Gene sequence:

>1668_bases
ATGTTACAAGAGATTTCAATCAAAAATTTTGCAATTATTGAAGAAATTCATCTATCCTTTGAGAGTGGAATGACGATTTT
AACTGGGGAAACTGGTGCGGGAAAATCAATAATTATTGATGCAATGTCTTTATTATTAGGTGGGCGTGCTTCGAGTGATT
TTGTGCGGCATGGGGCAAACAAAGCCGAAATAGAAGGTCTCTTTTTCTTTGATAAAACTCCAGAATTGAAGGCGGTATTA
TTAGAACTTGGCTTTGAAGAAGTAGATTCTGAAATTATTTTAAGACGTGAGATTTTTGCTAATGGTCGTTCCGTTTGTCG
AATCAATGGTCAAATGGTTAATTTAGCTACGCTAAGACAAGTGGGTGAATTATTAGTTGATATTCATGGTCAACACGATA
GCCAAGAATTGATGAATCCGAAAAGCCATCTTCGTTTACTTGATGAATTTGGTGACGAAGCCTTTGATCAGATAAAAAAT
AATTACAAACTCAAATTTGAAAACTTCAAAAATTTGCGTCAACAGCTCAATTTACGACAAAAAAATGAGCAAGAATTTGC
TCAAAGAATTGAAATTCTTCAATTTCAGGCTGAAGAAATTGAAGCGGCGGAAATTAATCTTGAAGAAGATGAACAATTAG
TTAATCGCCGAGAAAAGTTAAATAATATTAAAAATATTGCTGATTCGCTTAGTAGTGCTTATCTAGCGCTTGATGATGAA
GATAATGATTATTCAAGTCTTAATAATATCCGAACAACAATGACAGAATTGGATAAAATTAGTAACTTTGATAATGACTA
TCAAGAACTTGCTGACAAGACAGCTGAAAGTTATTATGTATTAGAAGAAGTTGCTAATCAAATTCAAAGAATCATGAGTG
ATTTAGAATTTAATCCAGCTGAACTTTTACAAATTGAAGACCGGATTATGACCTTGACCACTTTGAAGAAAAAATATGGG
CCAGAACTGTCCGATGTGATGAACTATTTAGAGAAAGTCCAACTTGAACTCTCAGAACTGACGGGTTCGGAAAATGACTC
AGAAAATCTAGAAAATTCAGTTAAAGTTGCACAAGTTCAACTCATTGAAGCAAGTAAAAAACTTAATGAAGCGCGCCATA
AAATTGCCAAAGAAGTTGAAAAAGATATTAAACACGAACTTTCTGAGCTTTATATGGAAAAAGCTGATTTCAAAGTTAGT
TTTGAAGGGGCAAAGTTTTCGGCAAACGGGAATGAGCATGTTGAATTTTTTATTCAAACCAATCCGGGAGAAGGATTTAA
ACCGCTGGCAAAAACAGCTTCGGGTGGTGAACTTTCACGTTTAATGCTCGCTATAAAATCAAGCTTTTCAAGACGTGAAA
ATAAAACAGCCATTGTTTTTGATGAGGTCGATACTGGTGTTTCTGGTCGTGTTGCTCAAGCGATTGCTAATAAAATATAT
AAAATTGCTAGACATGGGCAAGTTCTTGCAATTTCTCACCTTCCACAAGTTGTGGCCATTGCCGATAGACAATTTTATAT
TGAAAAAGCTTCAACCGATGACATTACTACTTCCACAGTTAGAAAATTAAACGCTGAAGAAAGAGTAAATGAAATTGCGA
AAATGCTTGCTGGTGAAGATTTAACTAAAGAAGCCTTAGCTCAGGCTAAACGTTTATTGAGCAAATAA

Upstream 100 bases:

>100_bases
CGAATTAATAGCTGTCAGTAAAAAAAGAATCAAAGTAGGAAAGATAAGGTTATTGGGTTACTAATAGCCTTGTCATTTAT
TTTTGAGAAAGGAAATTTTG

Downstream 100 bases:

>100_bases
ATCGAATATTATTAAGAAAAAGTCTTGATTTTTAATCAGGGCTTTTTATCTTTTAAATGACGAAATCGTAATTAGTGTAA
AATTGCAACCACAAATGTGT

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGANKAEIEGLFFFDKTPELKAVL
LELGFEEVDSEIILRREIFANGRSVCRINGQMVNLATLRQVGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDQIKN
NYKLKFENFKNLRQQLNLRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNNIKNIADSLSSAYLALDDE
DNDYSSLNNIRTTMTELDKISNFDNDYQELADKTAESYYVLEEVANQIQRIMSDLEFNPAELLQIEDRIMTLTTLKKKYG
PELSDVMNYLEKVQLELSELTGSENDSENLENSVKVAQVQLIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKVS
FEGAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIY
KIARHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNAEERVNEIAKMLAGEDLTKEALAQAKRLLSK

Sequences:

>Translated_555_residues
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGANKAEIEGLFFFDKTPELKAVL
LELGFEEVDSEIILRREIFANGRSVCRINGQMVNLATLRQVGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDQIKN
NYKLKFENFKNLRQQLNLRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNNIKNIADSLSSAYLALDDE
DNDYSSLNNIRTTMTELDKISNFDNDYQELADKTAESYYVLEEVANQIQRIMSDLEFNPAELLQIEDRIMTLTTLKKKYG
PELSDVMNYLEKVQLELSELTGSENDSENLENSVKVAQVQLIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKVS
FEGAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIY
KIARHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNAEERVNEIAKMLAGEDLTKEALAQAKRLLSK
>Mature_555_residues
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGANKAEIEGLFFFDKTPELKAVL
LELGFEEVDSEIILRREIFANGRSVCRINGQMVNLATLRQVGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDQIKN
NYKLKFENFKNLRQQLNLRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNNIKNIADSLSSAYLALDDE
DNDYSSLNNIRTTMTELDKISNFDNDYQELADKTAESYYVLEEVANQIQRIMSDLEFNPAELLQIEDRIMTLTTLKKKYG
PELSDVMNYLEKVQLELSELTGSENDSENLENSVKVAQVQLIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKVS
FEGAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIY
KIARHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNAEERVNEIAKMLAGEDLTKEALAQAKRLLSK

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=558, Percent_Identity=32.9749103942652, Blast_Score=310, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62797; Mature: 62797

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGAN
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFFFDKTPELKAVLLELGFEEVDSEIILRREIFANGRSVCRINGQMVNLATLRQ
HHHEEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCEEHHHHHHH
VGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDQIKNNYKLKFENFKNLRQQLNLRQ
HHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCHHHHHHHCCCCEEEHHHHHHHHHHHCCCC
KNEQEFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNNIKNIADSLSSAYLALDDE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
DNDYSSLNNIRTTMTELDKISNFDNDYQELADKTAESYYVLEEVANQIQRIMSDLEFNPA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ELLQIEDRIMTLTTLKKKYGPELSDVMNYLEKVQLELSELTGSENDSENLENSVKVAQVQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKVSFEGAKFSANGNEHVEFFIQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCCEEEEEEEE
NPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIY
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
KIARHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNAEERVNEIAKMLAGED
HHHHCCCEEEEHHCCHHHHHHCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCC
LTKEALAQAKRLLSK
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGAN
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFFFDKTPELKAVLLELGFEEVDSEIILRREIFANGRSVCRINGQMVNLATLRQ
HHHEEEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCEEHHHHHHH
VGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDQIKNNYKLKFENFKNLRQQLNLRQ
HHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCHHHHHHHCCCCEEEHHHHHHHHHHHCCCC
KNEQEFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNNIKNIADSLSSAYLALDDE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
DNDYSSLNNIRTTMTELDKISNFDNDYQELADKTAESYYVLEEVANQIQRIMSDLEFNPA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ELLQIEDRIMTLTTLKKKYGPELSDVMNYLEKVQLELSELTGSENDSENLENSVKVAQVQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKVSFEGAKFSANGNEHVEFFIQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCCEEEEEEEE
NPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIY
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
KIARHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNAEERVNEIAKMLAGED
HHHHCCCEEEEHHCCHHHHHHCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCC
LTKEALAQAKRLLSK
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]