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The map label for this gene is metS

Identifier: 15672774

GI number: 15672774

Start: 799071

End: 801059

Strand: Direct

Name: metS

Synonym: L0353

Alternate gene names: 15672774

Gene position: 799071-801059 (Clockwise)

Preceding gene: 15672770

Following gene: 15672775

Centisome position: 33.78

GC content: 35.8

Gene sequence:

>1989_bases
ATGACAGAAAACAAAACTTTTTACATTACAACCCCAATTTATTATCCATCAGGCAAGTTGCATCTTGGTAGTTCTTACAC
AACTATTGCTTGCGACGTGCTTGCTCGTTACAAACGTTTGATGGGCTTTGATACTTTTTATTTAACAGGGCTTGATGAAC
ACGGAATGAAAATTCAACGAAAAGCCGAAGAATTGGGAATGACTCCTAAAGAATACTTGGACCCAATGGCGGCTGATGTT
CAAGAATTATGGAAAAAATTGGATATTTCTTATGATAAATTTATCCGTACTACAGACACTTATCATGAAGAAGCCGTAGC
GAAGGCTTTTGAACAGTTATTGGAACAAGATGATATTTATTTGGGAAAATATGCGGGTTGGTATTCTGTTTCTGATGAAG
AGTTTTTCACTGAAACACAACTTGAAGAAATTTTCCGAGATGAATCTGGAAATATTACTGGTGGCATTGCACCTTCTGGT
CATGAAGTGGAATGGGTTGAAGAAGAAACTTATTTCTTCCGCATGGGAAAATATGCTGACTGGCTTTTACAATATTATGA
TGAACATCCAGATTTTATTCAACCTGAGGTTCGTAAAAATGAAATGGTCAATAATTTCATCAAGCCAGGTCTAGAAGATT
TGGCTTTGACACGGACAAGTTTTACTTGGGGAATTCCTGTCCCTTCTAATCCTAAGCATGTGGTCTACGTGTGGTTTGAT
GCTTTATTGAACTATATTACAGCGCTCGGCTATAATTCTGACAATGACAGTAATTTTAAAAAATACTGGCCTGGAATTAA
TATGGTTGGTAAAGAAATTGTTCGTTTCCATACGATTTACTGGCCAATTATGCTTCATGCTCTAGGTTTACCTGCTCCTA
AAAAGATTTTCGCTCATGGATGGCTCTTGATGAAAGACGGAAAAATGTCTAAATCAAAAGGAAATGTTGTTTATCCTGAG
ATGTTGATTGAACGTTATGGCCTTGATGCAGTTCGTTATTATTTGATGCGTGCGATTTCTTTTGGACAAGATGGAATTTT
CACTCCAGAAGACTTTGTTGGACGTATTAATTTTGATTTGGCCAATGATTTGGGGAATTTGTTGAATCGTACCGTTTCAA
TGATTAACAAATATAATGACGGAAAAATCGAAGCAACTGGAGTTTCGACTGAATTTGATGCAAGTTTGGAAGAAGTGGTT
GAAGAAACAATCAGTCATTTCCATAAAGCAATGGATAAATTTGAATTCAACGTAGCTTTGGCTGATGTTTGGACATTGAT
TTCAAGAACAAATAAATATATTGATGAAACAGCACCTTGGGTCCTTGCAAAATCAGAAGACGACAAAGCAAAATTGAATA
ATGTTTTGTATCATTTGGCAGAAAATTTACGGATTGCAGGTGCACTTTTACAACCATTTATGCGTGCAACATCAGGTAAA
ATCTTTGAGCAATTGGGAATGGATGAAAGATCATTTTCACTTGAAAATCTTTCTTTTGGCTATTCATTTACTCATCCTGT
AGTAGCAAAAGGTCAACCTATTTTCCCACGTCTTGATGTGGAAGAAGAGGTTGCTTATATTAAATTACAAATGGCTGGTG
GTGTATTGCCAGAAAAAGAGTGGGTCCCTGAAGAAGTTGAATTGAATTTAACTTTACCACAAATTAAATTTGATGATTTT
GAAAAAATTGAACTAAAAGTTGCCGAAGTGCTTGAAGTAGAACCTGTTGAAGGTTCTGATAAATTGTTGAGATTTAAATT
GGATGCTGGTGATTCTGAACCACGTCAAATCTTGTCAGGAATTGCACAATTCTATCCAAATGAACAAGAATTAGTAGGTA
AAAAATTACAAATCGTAGCCAATTTGAAACCACGTAAAATGATGAAAAAATACGTTTCACAAGGAATGATTTTGTCAGCC
GAATTTGATGGAAAATTGAGTGTCTTGACCGTTGATGATGATGTTCCTGCTGGTTCATTGATTGGGTAA

Upstream 100 bases:

>100_bases
AAACTTAATCAGACAATGCGATTTTAGAGGTTTTTTGGTATAATATTTAGATGAGAATCTTCTCAAATATCGAGCCTCAT
TTGCTCGGAAAGAGGAAAAT

Downstream 100 bases:

>100_bases
TAGCTAGAATAATTTACTGACAGACAATTTTGTTATGATAGTAAAAATGTTTTTTGCCGATGAGCGACAATGGTTTCTGT
CAGTAAAATGCGATTGAATA

Product: methyonyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MTENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQRKAEELGMTPKEYLDPMAADV
QELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIYLGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSG
HEVEWVEEETYFFRMGKYADWLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFAHGWLLMKDGKMSKSKGNVVYPE
MLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDLANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVV
EETISHFHKAMDKFEFNVALADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKEWVPEEVELNLTLPQIKFDDF
EKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSGIAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSA
EFDGKLSVLTVDDDVPAGSLIG

Sequences:

>Translated_662_residues
MTENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQRKAEELGMTPKEYLDPMAADV
QELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIYLGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSG
HEVEWVEEETYFFRMGKYADWLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFAHGWLLMKDGKMSKSKGNVVYPE
MLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDLANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVV
EETISHFHKAMDKFEFNVALADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKEWVPEEVELNLTLPQIKFDDF
EKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSGIAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSA
EFDGKLSVLTVDDDVPAGSLIG
>Mature_661_residues
TENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQRKAEELGMTPKEYLDPMAADVQ
ELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIYLGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSGH
EVEWVEEETYFFRMGKYADWLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFDA
LLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFAHGWLLMKDGKMSKSKGNVVYPEM
LIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDLANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVVE
ETISHFHKAMDKFEFNVALADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGKI
FEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKEWVPEEVELNLTLPQIKFDDFE
KIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSGIAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSAE
FDGKLSVLTVDDDVPAGSLIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=543, Percent_Identity=34.4383057090239, Blast_Score=298, Evalue=2e-80,
Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=25.3913043478261, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4507947, Length=132, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788432, Length=678, Percent_Identity=28.4660766961652, Blast_Score=252, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI212645096, Length=503, Percent_Identity=32.8031809145129, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=395, Percent_Identity=32.1518987341772, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17540794, Length=579, Percent_Identity=25.9067357512953, Blast_Score=157, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=31.6939890710383, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=480, Percent_Identity=25, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21358483, Length=504, Percent_Identity=31.7460317460317, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24655311, Length=556, Percent_Identity=24.8201438848921, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_LACLA (Q9CHE0)

Other databases:

- EMBL:   AE005176
- PIR:   H86723
- RefSeq:   NP_266948.1
- ProteinModelPortal:   Q9CHE0
- SMR:   Q9CHE0
- GeneID:   1114419
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0353
- NMPDR:   fig|272623.1.peg.812
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   LLAC272623:L0353-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75778; Mature: 75647

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQR
CCCCCEEEEECCEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
KAEELGMTPKEYLDPMAADVQELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIY
HHHHHCCCHHHHHCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
LGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSGHEVEWVEEETYFFRMGKYAD
EECCCCEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHEEEHHHHHH
WLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHEEECCEEECCCCCCCCCEEEEEHHH
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFAHG
HHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
WLLMKDGKMSKSKGNVVYPEMLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDL
EEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHEEECHHH
ANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVVEETISHFHKAMDKFEFNVAL
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKE
HHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHCEEEEEEEECCCCCCCCC
WVPEEVELNLTLPQIKFDDFEKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSG
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHH
IAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSAEFDGKLSVLTVDDDVPAGSL
HHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCC
IG
CC
>Mature Secondary Structure 
TENKTFYITTPIYYPSGKLHLGSSYTTIACDVLARYKRLMGFDTFYLTGLDEHGMKIQR
CCCCEEEEECCEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
KAEELGMTPKEYLDPMAADVQELWKKLDISYDKFIRTTDTYHEEAVAKAFEQLLEQDDIY
HHHHHCCCHHHHHCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
LGKYAGWYSVSDEEFFTETQLEEIFRDESGNITGGIAPSGHEVEWVEEETYFFRMGKYAD
EECCCCEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHEEEHHHHHH
WLLQYYDEHPDFIQPEVRKNEMVNNFIKPGLEDLALTRTSFTWGIPVPSNPKHVVYVWFD
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHEEECCEEECCCCCCCCCEEEEEHHH
ALLNYITALGYNSDNDSNFKKYWPGINMVGKEIVRFHTIYWPIMLHALGLPAPKKIFAHG
HHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
WLLMKDGKMSKSKGNVVYPEMLIERYGLDAVRYYLMRAISFGQDGIFTPEDFVGRINFDL
EEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHEEECHHH
ANDLGNLLNRTVSMINKYNDGKIEATGVSTEFDASLEEVVEETISHFHKAMDKFEFNVAL
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADVWTLISRTNKYIDETAPWVLAKSEDDKAKLNNVLYHLAENLRIAGALLQPFMRATSGK
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEQLGMDERSFSLENLSFGYSFTHPVVAKGQPIFPRLDVEEEVAYIKLQMAGGVLPEKE
HHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHCEEEEEEEECCCCCCCCC
WVPEEVELNLTLPQIKFDDFEKIELKVAEVLEVEPVEGSDKLLRFKLDAGDSEPRQILSG
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHH
IAQFYPNEQELVGKKLQIVANLKPRKMMKKYVSQGMILSAEFDGKLSVLTVDDDVPAGSL
HHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCC
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471