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The map label for this gene is icaA

Identifier: 15672662

GI number: 15672662

Start: 680427

End: 681491

Strand: Direct

Name: icaA

Synonym: L80399

Alternate gene names: 15672662

Gene position: 680427-681491 (Clockwise)

Preceding gene: 15672661

Following gene: 15672663

Centisome position: 28.76

GC content: 33.15

Gene sequence:

>1065_bases
ATGTTAATCTCAACAATGGTAGATGTTTATGTTGTTTATTATCCGATTATCTTGTCCTTCATTTGGGCAAGTGGTGCTTT
TTTATCTCGGTGGAAAGAAAGAGATAAGAAAGCAATTGATGAAACAAAAGAATTTGAAAAAATTAGTATTGTTATTTCTG
CGTACAATGAAGAAGAAACGATTGAGGAAGTTTTATTATCACTACGTAATCTAAATTACCCTTCCCTAGAGATCTTTATT
GTTGATGATAAGAGTAGTGACCGCACTTTAGAGAAACTTTATGAAGTAAAAAAATCTTTCGACGATTGGGAAACATTAAC
TATTCTTGAGCAAAAGGAAAATAAGGGAAAAGCCACGGCTTTAAATGTTGCTTTGCAACAAGTTAACAGTAAGTATATGC
TTGTTATTGATGCGGACTCATATCTTTCTAAAGATGCTCTATCTTATTTATTGGCCGAATTAACTTCTAGTTCAGATATT
GGGGCTGTAACTGGACGACCTATTGTTAGAAACAGAACAACTCTATTGGGGAAATTACAAACCTTAGAGTATTTAAGTGT
GATTGATGCGATTAAGAGAGCACAAAGTCTTTTTCTAGGTGCAATAATGACCGTATCAGGTGTGATTGTTATGTATCGTG
TAGAGGCTTTGAAAGAAATTGGGGGCTTTAATACCGAAGTGATGACGGAAGACATTGATGTCACTTGGCGACTTCATCGT
AATCAATGGAAAGTCAAATATGTCCCTAAAGCCTTAAGCTATATTCTCGTTCCTGAAAATTTAAAATCTCTTTTAAAGCA
ACGGAGAAGATGGGCAGTTGGTGGAGTAGAAGTATTAATTTCAAATTTATCATGGGTTTTTAAAAGAGGAGAATTTAAAC
ATCGGTTTCTTTTGATTGAAATGATTTGTAGTCATATTTGGAGTTGGTTTTTCTTAATAGAATCTTATCAGTATTTTCTC
CAAATGCTGTTAAATCAAGAATTCAAAGTATCGGGTCAAATTATCGTGATTTTTGTTCTTATTTCATTTTTTGTATTCTT
TTTAGGACCGCAAATTGTAAAATGA

Upstream 100 bases:

>100_bases
CCAGTAGCCTTTACTAGTGCGGCATTACTAGGAATTTTTGTGGGAAAAGTTTTAAATATCCCTTTTAAGAGAAAAAAAGA
AAGAAAGGAAAATTAAAACT

Downstream 100 bases:

>100_bases
AGTTAGCCACCTTGAGTCAAGCAAAAAACATCTGAATCTCTTATACTAGAAGTTCGACCAAATAGTATAAAAAGGAAATT
CAGATGCAAGACTATTCTAT

Product: glycosyl transferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLISTMVDVYVVYYPIILSFIWASGAFLSRWKERDKKAIDETKEFEKISIVISAYNEEETIEEVLLSLRNLNYPSLEIFI
VDDKSSDRTLEKLYEVKKSFDDWETLTILEQKENKGKATALNVALQQVNSKYMLVIDADSYLSKDALSYLLAELTSSSDI
GAVTGRPIVRNRTTLLGKLQTLEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFNTEVMTEDIDVTWRLHR
NQWKVKYVPKALSYILVPENLKSLLKQRRRWAVGGVEVLISNLSWVFKRGEFKHRFLLIEMICSHIWSWFFLIESYQYFL
QMLLNQEFKVSGQIIVIFVLISFFVFFLGPQIVK

Sequences:

>Translated_354_residues
MLISTMVDVYVVYYPIILSFIWASGAFLSRWKERDKKAIDETKEFEKISIVISAYNEEETIEEVLLSLRNLNYPSLEIFI
VDDKSSDRTLEKLYEVKKSFDDWETLTILEQKENKGKATALNVALQQVNSKYMLVIDADSYLSKDALSYLLAELTSSSDI
GAVTGRPIVRNRTTLLGKLQTLEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFNTEVMTEDIDVTWRLHR
NQWKVKYVPKALSYILVPENLKSLLKQRRRWAVGGVEVLISNLSWVFKRGEFKHRFLLIEMICSHIWSWFFLIESYQYFL
QMLLNQEFKVSGQIIVIFVLISFFVFFLGPQIVK
>Mature_354_residues
MLISTMVDVYVVYYPIILSFIWASGAFLSRWKERDKKAIDETKEFEKISIVISAYNEEETIEEVLLSLRNLNYPSLEIFI
VDDKSSDRTLEKLYEVKKSFDDWETLTILEQKENKGKATALNVALQQVNSKYMLVIDADSYLSKDALSYLLAELTSSSDI
GAVTGRPIVRNRTTLLGKLQTLEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFNTEVMTEDIDVTWRLHR
NQWKVKYVPKALSYILVPENLKSLLKQRRRWAVGGVEVLISNLSWVFKRGEFKHRFLLIEMICSHIWSWFFLIESYQYFL
QMLLNQEFKVSGQIIVIFVLISFFVFFLGPQIVK

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=351, Percent_Identity=34.4729344729345, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1786560, Length=243, Percent_Identity=30.0411522633745, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 41045; Mature: 41045

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISTMVDVYVVYYPIILSFIWASGAFLSRWKERDKKAIDETKEFEKISIVISAYNEEET
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHH
IEEVLLSLRNLNYPSLEIFIVDDKSSDRTLEKLYEVKKSFDDWETLTILEQKENKGKATA
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHEEEHHCCCCCCHHH
LNVALQQVNSKYMLVIDADSYLSKDALSYLLAELTSSSDIGAVTGRPIVRNRTTLLGKLQ
HHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
TLEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFNTEVMTEDIDVTWRLHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEC
NQWKVKYVPKALSYILVPENLKSLLKQRRRWAVGGVEVLISNLSWVFKRGEFKHRFLLIE
CCEEEEEHHHHHHHEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
MICSHIWSWFFLIESYQYFLQMLLNQEFKVSGQIIVIFVLISFFVFFLGPQIVK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHCC
>Mature Secondary Structure
MLISTMVDVYVVYYPIILSFIWASGAFLSRWKERDKKAIDETKEFEKISIVISAYNEEET
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHH
IEEVLLSLRNLNYPSLEIFIVDDKSSDRTLEKLYEVKKSFDDWETLTILEQKENKGKATA
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHEEEHHCCCCCCHHH
LNVALQQVNSKYMLVIDADSYLSKDALSYLLAELTSSSDIGAVTGRPIVRNRTTLLGKLQ
HHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
TLEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFNTEVMTEDIDVTWRLHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEC
NQWKVKYVPKALSYILVPENLKSLLKQRRRWAVGGVEVLISNLSWVFKRGEFKHRFLLIE
CCEEEEEHHHHHHHEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
MICSHIWSWFFLIESYQYFLQMLLNQEFKVSGQIIVIFVLISFFVFFLGPQIVK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA