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The map label for this gene is gatA

Identifier: 15672145

GI number: 15672145

Start: 165635

End: 167095

Strand: Direct

Name: gatA

Synonym: L0473

Alternate gene names: 15672145

Gene position: 165635-167095 (Clockwise)

Preceding gene: 15672144

Following gene: 15672146

Centisome position: 7.0

GC content: 37.78

Gene sequence:

>1461_bases
ATGTCATACAATAACAAATCAATCAAAGAGCTTCATGAGCTACTTGTAAATAAAGAAATTTCTGCTCTTGAACTGACAAA
AGCAACGCTTTCTGATATTTCTGCACGTGAACCACAAATTGATGCTTTTTTGAAAATTACAGAAGAAAAAGCTTTGCAAG
ATGCCGCCGCAATTGATGCTCGTGGAATTAATCCAGACGTTGTGACTGACGGAATCTCAATTGCTGTCAAAGATAATATT
GTTACAGAAGGAATTGAAACAACAGCTGCTTCAAAAATTCTTGGAGGTTGGATTCCACCTTATAATGCAACGGTTGCGAA
TAAATTGAGCGAATCTGGGTTAATTACAATTGGTAAACTCAATATGGATGAGTTTGCGATGGGTGGTTCTGGTGAAAATT
CTTCAGTTAAGCCAACCAAAAACGCTTGGGATCAAACAAAAGTACCTGGTGGTTCTTCATCTGGTTCTGCGGCTTCAGTT
GCTTCTGGTCAAGTTCGTTTATCATTGGGTTCAGATACTGGTGGCTCAATTCGCCAACCTGCCGCATTCAATGGGATTGT
TGGTTTGAAACCAACTTATGGTCGTGTGAGTCGTTTTGGTTTAATTGCTTTTGCTTCATCATTAGACCAAATCGGTCCAT
TGGCGCCAACAGTCGAAGAAAATGCTCAACTTTTGAATGTCATTTCTGGCTTTGATAAAAATGATAGCACATCATCAAAT
GTTGCAGTTCCTGACTTTACAAGTAAAATTGGTCAAGACATTAAAGGAATGAAGATTGCTTTGCCTAAAGAATATTTTGG
TGAAGGAATTGATGAAAAAGTAAAAGAACAAATTTTGGCAGCAGCTAAACATTTGGAAAAATTGGGTGCCATTGTTGAAG
AAGTTAGTCTTCCTCACAGTAAATATGGTGTTGCAGTTTATTATATTATTGCCTCTTCTGAAGCCAGCTCAAATCTTCAA
CGTTTTGATGGTATTCGGTATGGTTATCGTGCACAAGACATCAAAAATCTTGAAGATTTGTATGTGAAATCACGTTCTGA
AGGATTTGGTCCAGAAGTTCAACGTCGAATTATGTTAGGAACTTTCAGTCTTTCAGCAGGTTCTTATGATAAACATTTCA
AAAAAGCAGGTCAAGTTCGTACTTTAATCATCAATGATTTTGCAAAAGTTTTTGAAAAATATGATTTAATCTTAGGCCCA
ACAACTCCGACACCAGCTTGGGACTTGGGAGCACGTGTTGATGATCCACTTTCAATGTATTTGGCGGATTTATTGACAAT
TCCAGTCAACCTAGCTGGCCTTCCAGGAATTTCAATTCCTGCTGGTTTTGCCGATGGACTACCTGTGGGAATGCAATTGA
TTGGTAAACGTTACGATGAAGAAACGATTTATAAAGTAGCGGCTGCTTTTGAAGCAACTACTGATTTCCACAAAAAACAA
CCGATTATTTTTGGAAAATAA

Upstream 100 bases:

>100_bases
AAAAAGGACTTGACCGTGAAAAACTCATGGCAGCCGTTCCTGAAAAGGAAGACGGCTTTATCAAAGTTCCAGCAATGCTT
TCTGATGGAGGAGATGCTTA

Downstream 100 bases:

>100_bases
AAAATGGAATTTGACGAATTAATCAAGCGATTAAATGCAGTTGCTGATGCAGGACTTTTTTACGGGAAAGATGTCTTTGA
TATTGAACGTTATCATGAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSYNNKSIKELHELLVNKEISALELTKATLSDISAREPQIDAFLKITEEKALQDAAAIDARGINPDVVTDGISIAVKDNI
VTEGIETTAASKILGGWIPPYNATVANKLSESGLITIGKLNMDEFAMGGSGENSSVKPTKNAWDQTKVPGGSSSGSAASV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLAPTVEENAQLLNVISGFDKNDSTSSN
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGP
TTPTPAWDLGARVDDPLSMYLADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYKVAAAFEATTDFHKKQ
PIIFGK

Sequences:

>Translated_486_residues
MSYNNKSIKELHELLVNKEISALELTKATLSDISAREPQIDAFLKITEEKALQDAAAIDARGINPDVVTDGISIAVKDNI
VTEGIETTAASKILGGWIPPYNATVANKLSESGLITIGKLNMDEFAMGGSGENSSVKPTKNAWDQTKVPGGSSSGSAASV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLAPTVEENAQLLNVISGFDKNDSTSSN
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGP
TTPTPAWDLGARVDDPLSMYLADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYKVAAAFEATTDFHKKQ
PIIFGK
>Mature_485_residues
SYNNKSIKELHELLVNKEISALELTKATLSDISAREPQIDAFLKITEEKALQDAAAIDARGINPDVVTDGISIAVKDNIV
TEGIETTAASKILGGWIPPYNATVANKLSESGLITIGKLNMDEFAMGGSGENSSVKPTKNAWDQTKVPGGSSSGSAASVA
SGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLAPTVEENAQLLNVISGFDKNDSTSSNV
AVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPT
TPTPAWDLGARVDDPLSMYLADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYKVAAAFEATTDFHKKQP
IIFGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=37.7510040160643, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI195972892, Length=484, Percent_Identity=23.1404958677686, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=38.1609195402299, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=478, Percent_Identity=23.6401673640167, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=31.0160427807487, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=208, Percent_Identity=29.8076923076923, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=231, Percent_Identity=24.6753246753247, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=35.6626506024096, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=30.8045977011494, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=39.9201596806387, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652985, Length=484, Percent_Identity=24.7933884297521, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19922090, Length=484, Percent_Identity=24.7933884297521, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652981, Length=484, Percent_Identity=24.7933884297521, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652983, Length=484, Percent_Identity=24.7933884297521, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=23.8747553816047, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=34.375, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=34.375, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=34.375, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=23.4939759036145, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648441, Length=127, Percent_Identity=39.3700787401575, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648439, Length=127, Percent_Identity=39.3700787401575, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356731, Length=340, Percent_Identity=24.7058823529412, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_LACLA (Q9CJ43)

Other databases:

- EMBL:   AE005176
- PIR:   C86645
- RefSeq:   NP_266319.1
- ProteinModelPortal:   Q9CJ43
- SMR:   Q9CJ43
- GeneID:   1113771
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0473
- NMPDR:   fig|272623.1.peg.170
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   LLAC272623:L0473-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52072; Mature: 51941

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNNKSIKELHELLVNKEISALELTKATLSDISAREPQIDAFLKITEEKALQDAAAIDA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
RGINPDVVTDGISIAVKDNIVTEGIETTAASKILGGWIPPYNATVANKLSESGLITIGKL
CCCCCCCEECCEEEEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEC
NMDEFAMGGSGENSSVKPTKNAWDQTKVPGGSSSGSAASVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLAPTVEENAQLLNVISGFDKNDSTSSN
CHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHS
EECCCHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEEEE
TFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPTTPTPAWDLGARVDDPLSMY
EEECCCCCHHHHHHHCCCEEEHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYKVAAAFEATTDFHKKQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PIIFGK
CCCCCC
>Mature Secondary Structure 
SYNNKSIKELHELLVNKEISALELTKATLSDISAREPQIDAFLKITEEKALQDAAAIDA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
RGINPDVVTDGISIAVKDNIVTEGIETTAASKILGGWIPPYNATVANKLSESGLITIGKL
CCCCCCCEECCEEEEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEC
NMDEFAMGGSGENSSVKPTKNAWDQTKVPGGSSSGSAASVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLAPTVEENAQLLNVISGFDKNDSTSSN
CHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHS
EECCCHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEEEE
TFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPTTPTPAWDLGARVDDPLSMY
EEECCCCCHHHHHHHCCCEEEHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYKVAAAFEATTDFHKKQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PIIFGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471