Click here to switch to the map view.

The map label for this gene is tra1077B

Identifier: 15672121

GI number: 15672121

Start: 139171

End: 139989

Strand: Direct

Name: tra1077B

Synonym: L0467

Alternate gene names: 15672121

Gene position: 139171-139989 (Clockwise)

Preceding gene: 15672120

Following gene: 15672124

Centisome position: 5.88

GC content: 34.55

Gene sequence:

>819_bases
TTGAAAGTTACAGGATTCCCTAAAGCCACTTATTATTATTGGGTTAACTGTTTTGAACGGGTAAACAAAGATGAGCTTAT
CGAAAAAGAAATGCTTAAAATACGTCAAGAACATGCCAATGCAGGTTATCGTCCAATGAGTGAATTACTTAAGCAACGTG
GCTATCACGTCAACCACAAGAAGGTACAGCGCTTAATGAAGAAGCTAGGGCTTCGTGTGACGTCTTATTGGCACAAATTA
CGTAATCATAATTCATATAAAGGAAAAGTAGGGACGGTAGCTAAAAATAAATTGCACAGACGCTTCAGAACTTCCATTCC
TCATCAAAAAATCACAACAGATACGACAGAATTCAAATATTATGAAGATGGAATTCAGAAAAAGTGTTATCTCAATCCTT
ACATTGATTTATTTAATAGCGAGGTGATTAGTTATCATATCTCTAAACACCCCTCTTATCAATCAATTGATATTGCTCTA
AATCAAGCCTTAGCTGTGACATCTGATTGTCCTTATCGACGTACTTTCCACTCAGATCAAGGATGGGGATACCAAATGAG
AGACTATGTTTCTAAATTAAAATCTCATAGAATTTTTCAGTCTATGAGCCGTAAAGGAAAATGTCATGATAATTCAGTTA
TGGAGAATTTCTTTGGGCTACTTAAACAAGAAATTTACTACGGACATATCTTCTCGTCTTTCGAAGAACTTGAACAGGTT
ATTGTAATCTGGATTAGGTACTATAATACAAAACGAATCAAACAAAAATTGAACTGGATGAGTCCAATTCAATTTCGCTT
GAATTACCAAAACAATTAA

Upstream 100 bases:

>100_bases
CTTATTTAAAAGGACTTCGGAGATTGGAGAAAAATGCTCTAATGAACAAAAATCAAGACTCATCTACAGTCTCCGTAAAA
CCTTCAAACTCAAAGAAATC

Downstream 100 bases:

>100_bases
AAAAATCCAAGTATTTGGAAAGTCAACGAAAAACTAGACACGGAGTTAAGAGAATTTAAGAATTATATTTTTCAAAGTCT
TTTGGAGACTTGTAATCAAG

Product: IS1077B transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMSELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKL
RNHNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKHPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGKCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

Sequences:

>Translated_272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMSELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKL
RNHNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKHPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGKCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN
>Mature_272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMSELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKL
RNHNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKHPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGKCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=268, Percent_Identity=41.044776119403, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1788405, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787263, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786753, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786570, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786490, Length=273, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32797; Mature: 32797

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]